BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,541,609	G→T	5.6%	E53* (GAA→TAA)	uxuA →	mannonate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,541,609	0	G	T	5.6%	1027.9 / 27.2	269	E53* (GAA→TAA)	uxuA	mannonate hydrolase
Reads supporting (aligned to +/- strand): ref base G (116/138); new base T (9/6); total (125/144)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 272 total aligned reads displayed.

AGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCGTGTGCTAC  >  CP009273/4541460‑4541758

 gACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGa                                                                                                                                                      >  2:1835295/1‑150 (MQ=255)

 gACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGa                                                                                                                                                      <  2:1369592/150‑1 (MQ=255)

   ccTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAg                                                                                                                                                    >  1:3930799/1‑150 (MQ=255)

      ggCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACg                                                                                                                                                 <  2:3554980/150‑1 (MQ=255)

      ggCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACg                                                                                                                                                 >  2:1684957/1‑150 (MQ=255)

      ggCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACg                                                                                                                                                 <  1:3079354/150‑1 (MQ=255)

       gcgcTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGc                                                                                                                                                <  1:3715104/150‑1 (MQ=255)

       gcgcTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGAGGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATCTCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGc                                                                                                                                                <  1:3715129/150‑1 (MQ=255)

        cgcTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCa                                                                                                                                               <  2:3031856/150‑1 (MQ=255)

          cTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAgg                                                                                                                                             <  2:3889746/150‑1 (MQ=255)

           tGGTACGGCCCAAACGATCCGGTTTATTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGc                                                                                                                                            <  1:563419/150‑1 (MQ=255)

           tGGTACGGCCCAAACGATCCGGTTTATTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGc                                                                                                                                            <  1:2730976/150‑1 (MQ=255)

             gTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCt                                                                                                                                          >  1:2161901/1‑150 (MQ=255)

                cGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGgt                                                                                                                                       >  2:3498642/1‑150 (MQ=255)

                cGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGgt                                                                                                                                       >  2:431451/1‑150 (MQ=255)

                 ggCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGgtg                                                                                                                                      <  2:3117969/150‑1 (MQ=255)

                      aaaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTc                                                                                                                                 >  2:2406387/1‑150 (MQ=255)

                       aaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCt                                                                                                                                <  1:2612241/150‑1 (MQ=255)

                       aaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCt                                                                                                                                <  2:2027395/150‑1 (MQ=255)

                       aaCGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCt                                                                                                                                <  2:1948062/150‑1 (MQ=255)

                       aaCGATCCGGGTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCt                                                                                                                                <  1:2612025/150‑1 (MQ=255)

                         cGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGt                                                                                                                              <  2:2167542/150‑1 (MQ=255)

                           aTCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTTAAGACGCAGGCCTGGTGTGGTCTGTCg                                                                                                                            <  2:1879359/150‑1 (MQ=255)

                              cGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAg                                                                                                                         >  1:2719322/1‑150 (MQ=255)

                                 tttCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGaaa                                                                                                                      <  2:3969676/150‑1 (MQ=255)

                                   tCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGc                                                                                                                    <  2:3696156/150‑1 (MQ=255)

                                      ttAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTg                                                                                                                 <  1:2450504/150‑1 (MQ=255)

                                       tAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGc                                                                                                                >  1:3629051/1‑150 (MQ=255)

                                        aGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGcc                                                                                                               >  1:1399097/1‑150 (MQ=255)

                                           tGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAAt                                                                                                            >  1:1017636/1‑150 (MQ=255)

                                              tGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCa                                                                                                         >  2:578954/1‑150 (MQ=255)

                                               gTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCAc                                                                                                        >  1:4261425/1‑150 (MQ=255)

                                                tCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACg                                                                                                       <  1:4105898/150‑1 (MQ=255)

                                                  cGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGaa                                                                                                     >  2:2470047/1‑150 (MQ=255)

                                                   gTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAg                                                                                                    >  1:2422128/1‑150 (MQ=255)

                                                      aGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGata                                                                                                 >  1:724458/1‑150 (MQ=255)

                                                      aGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGata                                                                                                 <  1:1834972/150‑1 (MQ=255)

                                                      aGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGata                                                                                                 >  2:522427/1‑150 (MQ=255)

                                                         cgggcgCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCa                                                                                              <  2:3704365/150‑1 (MQ=255)

                                                         cgggcgCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCa                                                                                              >  2:82225/1‑150 (MQ=255)

                                                          gggcgCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCGAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCaa                                                                                             >  1:1437867/1‑150 (MQ=255)

                                                              gcAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAAcc                                                                                         <  1:4252751/150‑1 (MQ=255)

                                                              gcAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAAcc                                                                                         <  1:4199984/150‑1 (MQ=255)

                                                                 aCTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAATATATCAAAACCcac                                                                                      <  2:1168637/150‑1 (MQ=255)

                                                                  cTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCcaca                                                                                     <  1:3515194/150‑1 (MQ=255)

                                                                    ggCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTTAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACt                                                                                   <  2:1331983/150‑1 (MQ=255)

                                                                     gCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTg                                                                                  <  1:2020905/150‑1 (MQ=255)

                                                                        tGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCa                                                                               <  1:4163734/150‑1 (MQ=255)

                                                                           ttACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTGGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACt                                                                            <  2:1534628/150‑1 (MQ=255)

                                                                           ttACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACt                                                                            <  1:4323868/150‑1 (MQ=255)

                                                                           ttACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACt                                                                            <  2:1739160/150‑1 (MQ=255)

                                                                             aCCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTAt                                                                          >  2:1406845/1‑150 (MQ=255)

                                                                              ccGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATg                                                                         <  2:1759355/150‑1 (MQ=255)

                                                                                 cgcTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGc                                                                      >  1:93031/1‑150 (MQ=255)

                                                                                 cgcTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGc                                                                      <  2:2189595/150‑1 (MQ=255)

                                                                                   cTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAg                                                                    >  1:2263632/1‑150 (MQ=255)

                                                                                    tGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGt                                                                   >  1:437569/1‑150 (MQ=255)

                                                                                     gCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTg                                                                  <  2:1632063/150‑1 (MQ=255)

                                                                                      cACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTgg                                                                 >  2:1433369/1‑150 (MQ=255)

                                                                                           tatCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGc                                                            >  1:3359242/1‑150 (MQ=255)

                                                                                            atCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTTAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCt                                                           >  2:3504396/1‑150 (MQ=255)

                                                                                            atCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCt                                                           >  2:775129/1‑150 (MQ=255)

                                                                                            atCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCt                                                           <  1:4293918/150‑1 (MQ=255)

                                                                                            atCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCt                                                           <  1:757109/150‑1 (MQ=255)

                                                                                                 gAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTTAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTa                                                      >  2:2251605/1‑150 (MQ=255)

                                                                                                 gAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTa                                                      <  2:2098668/150‑1 (MQ=255)

                                                                                                 gAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTa                                                      <  2:4286347/150‑1 (MQ=255)

                                                                                                   aCGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATc                                                    <  2:20436/150‑1 (MQ=255)

                                                                                                    cGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTTAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATca                                                   >  2:3726048/1‑150 (MQ=255)

                                                                                                        gAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATcagcag                                               >  2:1008764/1‑150 (MQ=255)

                                                                                                          aGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGAc                                             <  2:2121815/150‑1 (MQ=255)

                                                                                                          aGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGAc                                             >  1:381580/1‑150 (MQ=255)

                                                                                                              tGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTg                                         >  2:3386532/1‑150 (MQ=255)

                                                                                                                   cGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCaa                                    >  2:2116314/1‑150 (MQ=255)

                                                                                                                   cGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCaa                                    >  2:2334745/1‑150 (MQ=255)

                                                                                                                    gTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAAc                                   >  1:3254301/1‑150 (MQ=255)

                                                                                                                     ttGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAAcc                                  <  1:852991/150‑1 (MQ=255)

                                                                                                                      tGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGATCAGTGGATAGCTAACTATCAGCAGACTCTGCGCAAcat                                 >  2:2607508/1‑148 (MQ=255)

                                                                                                                      tGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCt                                 >  1:1284490/1‑150 (MQ=255)

                                                                                                                       gAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTg                                <  2:4176501/150‑1 (MQ=255)

                                                                                                                        aaGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTgg                               <  2:3297854/150‑1 (MQ=255)

                                                                                                                         agagATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGgc                              >  2:408682/1‑150 (MQ=255)

                                                                                                                         agagATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGgc                              >  2:980344/1‑150 (MQ=255)

                                                                                                                         agagATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGgc                              >  2:2583823/1‑150 (MQ=255)

                                                                                                                         agagATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGgc                              >  1:2651725/1‑150 (MQ=255)

                                                                                                                         agagATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGgc                              <  1:492385/150‑1 (MQ=255)

                                                                                                                            gaTCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCa                           >  1:3094334/1‑150 (MQ=255)

                                                                                                                               ccTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAgtg                        <  2:2373021/150‑1 (MQ=255)

                                                                                                                                  cAAACGCAAGGCGATCATTTAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTgg                     <  1:1156199/150‑1 (MQ=255)

                                                                                                                                      cGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAATCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCAtt                 <  1:25404/150‑1 (MQ=255)

                                                                                                                                          aGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCa             >  1:3294273/1‑150 (MQ=255)

                                                                                                                                          aGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCa             >  2:2512496/1‑150 (MQ=255)

                                                                                                                                          aGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCa             >  2:757707/1‑150 (MQ=255)

                                                                                                                                            gCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCAcc           <  2:2146404/150‑1 (MQ=255)

                                                                                                                                              gATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCgt         >  1:2919635/1‑150 (MQ=255)

                                                                                                                                              gATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCgt         >  1:2336079/1‑150 (MQ=255)

                                                                                                                                              gATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCgt         >  1:165104/1‑150 (MQ=255)

                                                                                                                                               aTCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCgtg        <  1:2819980/150‑1 (MQ=255)

                                                                                                                                                  aTTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCGTGTGc     >  2:1134067/1‑150 (MQ=255)

                                                                                                                                                     gAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCGTGTGCTAc  >  2:2950631/1‑150 (MQ=255)

AGACCTGGCGCTGGTACGGCCCAAACGATCCGGTTTCTTTAGCTGATGTCCGTCAGGCGGGCGCAACTGGCGTGGTTACCGCGCTGCACCATATCCCGAACGGCGAAGTATGGTCCGTTGAAGAGATCCTCAAACGCAAGGCGATCATTGAAGACGCAGGCCTGGTGTGGTCTGTCGTAGAAAGCGTGCCAATTCACGAAGATATCAAAACCCACACTGGCAACTATGAGCAGTGGATCGCTAACTATCAGCAGACTCTGCGCAACCTGGCGCAGTGTGGCATTCGCACCGTGTGCTAC  >  CP009273/4541460‑4541758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`