Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,571,233 G→A 46.7% S16F (TCT→TTT)  hsdS ← specificity determinant for hsdM and hsdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,571,2330GA46.7% 61.0 / 311.5 204S16F (TCT→TTT) hsdSspecificity determinant for hsdM and hsdR
Reads supporting (aligned to +/- strand):  ref base G (70/38);  new base A (62/33);  total (132/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

ATTTTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCT  >  CP009273/4571085‑4571382
                                                                                                                                                    |                                                                                                                                                     
   tttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATAc                                                                                                                                                   >  1:3724532/1‑150 (MQ=255)
    ttAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACt                                                                                                                                                  <  1:2503393/150‑1 (MQ=255)
     tAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTg                                                                                                                                                 >  1:4252132/1‑150 (MQ=255)
     tAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTg                                                                                                                                                 <  2:584967/150‑1 (MQ=255)
     tAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTCACTCCTCGGATTAGAGTTGTGACCGTAAATACTg                                                                                                                                                 >  1:1430860/1‑150 (MQ=255)
           cAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGa                                                                                                                                           >  1:2585960/1‑150 (MQ=255)
            aaaaaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGAt                                                                                                                                          >  1:1460442/1‑150 (MQ=255)
             aaaaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATa                                                                                                                                         <  2:2817027/150‑1 (MQ=255)
              aaaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATaa                                                                                                                                        >  1:2615336/1‑150 (MQ=255)
                        cGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTc                                                                                                                              <  2:394121/150‑1 (MQ=255)
                            gtaTCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGc                                                                                                                          >  1:2526753/1‑150 (MQ=255)
                               tCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAAt                                                                                                                       <  2:530311/150‑1 (MQ=255)
                                    cTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTccc                                                                                                                  <  1:68922/150‑1 (MQ=255)
                                    cTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTccc                                                                                                                  >  2:1726951/1‑150 (MQ=255)
                                     ttGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTcccc                                                                                                                 <  1:407166/150‑1 (MQ=255)
                                        ccATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCa                                                                                                              <  1:1528119/150‑1 (MQ=255)
                                        ccATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCa                                                                                                              >  2:2416925/1‑150 (MQ=255)
                                          aTTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACt                                                                                                            <  2:3865073/150‑1 (MQ=255)
                                             cTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATAAGTTACTACTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACACACCCCTCCGGCAATTTCCCAGAACTCAt                                                                                                         >  1:4170557/1‑150 (MQ=255)
                                               gAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTc                                                                                                       >  2:790192/1‑150 (MQ=255)
                                               gAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCACCGGCAATTTCCCCGCACTCATTc                                                                                                       >  1:1016730/1‑150 (MQ=255)
                                               gAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTGTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTc                                                                                                       >  2:791684/1‑150 (MQ=255)
                                                   atTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACACATTCCTTc                                                                                                   >  2:4013569/1‑150 (MQ=255)
                                                    ttGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTATATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCa                                                                                                  <  2:1915787/150‑1 (MQ=255)
                                                     tGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCAc                                                                                                 <  1:98138/150‑1 (MQ=255)
                                                        tCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTACTTCAcccc                                                                                              >  2:1036201/1‑150 (MQ=255)
                                                           cACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTACCCGCACACATTCCTTCAACccacc                                                                                           >  2:1108401/1‑150 (MQ=255)
                                                           cACGGATAAGAGGCAAATAATCATCTTGTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAAGTTCCCCGCACTCAGTCCTTCACCccacc                                                                                           >  1:3486055/1‑150 (MQ=255)
                                                            aCGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAGTCCTTCACCccacca                                                                                          >  1:1289069/1‑150 (MQ=255)
                                                              ggATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCaaa                                                                                        >  1:2749247/1‑150 (MQ=255)
                                                              ggATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCaaa                                                                                        >  1:626130/1‑150 (MQ=255)
                                                               gATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTACCCGCACTCATTCCTTCACCCCACCAAAc                                                                                       >  2:2122278/1‑150 (MQ=255)
                                                               gATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAAc                                                                                       >  2:1462312/1‑150 (MQ=255)
                                                                     agGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttct                                                                                 >  2:2009956/1‑150 (MQ=255)
                                                                      ggCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                                <  2:194390/150‑1 (MQ=255)
                                                                      ggCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                                <  1:1357059/150‑1 (MQ=255)
                                                                      ggCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                                <  2:710350/150‑1 (MQ=255)
                                                                      ggCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTCACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGcttctt                                                                                <  2:1430860/150‑1 (MQ=255)
                                                                       gCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCttcttc                                                                               <  2:146693/150‑1 (MQ=255)
                                                                          aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTAACCCCACCCAACGCTTCTTCCAg                                                                            >  1:785074/1‑150 (MQ=255)
                                                                           ataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAAACCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGc                                                                           >  2:1303532/1‑150 (MQ=255)
                                                                           ataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCACCGGCAAATTCCCCGCACACATTCCATCACCCCACCAAACCCTTCTTCCAGc                                                                           >  1:2892088/1‑150 (MQ=255)
                                                                            taatCATCTTTTAGAGAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCACCGGCAAATTCCCCCCACGCATTCCATCACCCCACCAAACGCGTCTTCCCGcc                                                                          >  1:3004407/1‑149 (MQ=255)
                                                                                 atcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGa                                                                     <  2:2451419/150‑1 (MQ=255)
                                                                                  tcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGAc                                                                    >  1:3896722/1‑150 (MQ=255)
                                                                                   cTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAAATTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACg                                                                   >  2:2251985/1‑150 (MQ=255)
                                                                                     tttAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCt                                                                 <  1:536591/150‑1 (MQ=255)
                                                                                     tttAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAAATTCCCCGCACGCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCt                                                                 >  2:1438927/1‑150 (MQ=255)
                                                                                       tAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCCGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGc                                                               >  1:2051645/1‑150 (MQ=255)
                                                                                        aGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCACCCGCAATTTCCCCCCACTCATTCCTTCACCCCACCCAAAGCATATTCCAGCAACTGACCCTGCa                                                              >  1:3248787/1‑150 (MQ=255)
                                                                                             aTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                                         <  2:3768663/150‑1 (MQ=255)
                                                                                             aTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                                         <  2:1250940/150‑1 (MQ=255)
                                                                                               ttATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCCGCAATTTACCCGCACTCATTCCGTCACCCCACCAAACGCTTCTTCCAGCAACAGACGCTGCAAATCGGc                                                       >  1:3603038/1‑150 (MQ=255)
                                                                                                tATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGcc                                                      >  1:4123447/1‑150 (MQ=255)
                                                                                                  ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTc                                                    <  2:2375323/150‑1 (MQ=255)
                                                                                                  ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTc                                                    <  2:296599/150‑1 (MQ=255)
                                                                                                  ttGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTc                                                    <  1:1977252/150‑1 (MQ=255)
                                                                                                   tGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCCCTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCGGCAAATCGGCCTCa                                                   >  2:1747431/1‑150 (MQ=255)
                                                                                                   tGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                                   >  2:1747410/1‑150 (MQ=255)
                                                                                                    gCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAt                                                  >  2:424218/1‑150 (MQ=255)
                                                                                                    gCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTGCCAGCAACTGACGCTGCAAATCGGCCTCAt                                                  >  2:3969382/1‑150 (MQ=255)
                                                                                                    gCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCATCTTCCAGCAACTGACGCTGCCAATCGGCCTCAt                                                  >  2:4017924/1‑150 (MQ=255)
                                                                                                     ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtc                                                 >  2:3343609/1‑150 (MQ=255)
                                                                                                     ccTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCATCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtc                                                 >  2:3599495/1‑150 (MQ=255)
                                                                                                      cTGCTCTTTTTGAGACGGTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcg                                                <  2:2707592/150‑1 (MQ=255)
                                                                                                       tGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAGTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgc                                               >  2:3027679/1‑150 (MQ=255)
                                                                                                         ctctTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgctc                                             >  1:4199962/1‑150 (MQ=255)
                                                                                                          tctTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAGATCGGCCTCAtcgctcg                                            <  2:1226692/150‑1 (MQ=255)
                                                                                                               tttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGccccc                                       >  1:1827031/1‑150 (MQ=255)
                                                                                                                ttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCa                                      <  1:4028534/150‑1 (MQ=255)
                                                                                                                ttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCa                                      <  2:2914148/150‑1 (MQ=255)
                                                                                                                  ataCGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCGCATCGCTCGCCCCCAGt                                    >  1:1197950/1‑150 (MQ=255)
                                                                                                                  ataCGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCACCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGt                                    >  1:1197940/1‑150 (MQ=255)
                                                                                                                   taCGTTACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGtt                                   <  1:1954378/150‑1 (MQ=255)
                                                                                                                   taCGTTACTCCTCGGATTAGAGGTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCGTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGtt                                   >  2:3260817/1‑150 (MQ=255)
                                                                                                                     cGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCa                                 >  2:382576/1‑150 (MQ=255)
                                                                                                                     cGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCa                                 >  1:3161826/1‑150 (MQ=255)
                                                                                                                       ttACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACg                               >  2:3696614/1‑150 (MQ=255)
                                                                                                                        tACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGc                              <  1:443648/150‑1 (MQ=255)
                                                                                                                        tACTCCTCGGATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCGTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGc                              >  2:2483472/1‑150 (MQ=255)
                                                                                                                           tcctcGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATc                           >  2:3367120/1‑150 (MQ=255)
                                                                                                                            cctcGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCATCCAGCAACAGACGCTGCAAATCGGCCACATCGCTCGCCCCCAGTTAACGCATCa                          >  2:4149971/1‑150 (MQ=255)
                                                                                                                             ctcGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg                         >  1:3387330/1‑150 (MQ=255)
                                                                                                                             ctcGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg                         <  1:1554390/150‑1 (MQ=255)
                                                                                                                             ctcGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg                         >  1:3387355/1‑150 (MQ=255)
                                                                                                                                ggATTAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAgcgc                      >  1:1835666/1‑150 (MQ=255)
                                                                                                                                   ttAGAGTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATc                   <  2:2387949/150‑1 (MQ=255)
                                                                                                                                     agagTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCAACACCGCATCCa                 >  2:2539361/1‑150 (MQ=255)
                                                                                                                                       agTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCCCTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGt               >  1:2212095/1‑150 (MQ=255)
                                                                                                                                       agTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAAATTCCCCGCACACATTCCTTCACCCCACCCAACGCTTCTTCCAGCAACAGACGCTGCAAATCGGCCACATCGCTCGCCCCCAGTTCACGCATCAGCGCAACCAGt               >  1:3355592/1‑150 (MQ=255)
                                                                                                                                       agTTGTGACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCATCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGt               >  2:3517061/1‑150 (MQ=255)
                                                                                                                                        gTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGtt              <  1:2817972/150‑1 (MQ=255)
                                                                                                                                          tgtgACCGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCCGTTCa            >  2:3443604/1‑150 (MQ=255)
                                                                                                                                           gtgACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAg           <  2:2778534/150‑1 (MQ=255)
                                                                                                                                            tgACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGa          >  2:1179280/1‑150 (MQ=255)
                                                                                                                                                cGTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCCCTCATTCCGTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAgc      >  2:3187132/1‑150 (MQ=255)
                                                                                                                                                 gTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAgcg     <  1:2123989/150‑1 (MQ=255)
                                                                                                                                                 gTAAATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTACTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCCTCAGCGCATCCAGTTCAGACAgcg     >  1:2931343/1‑150 (MQ=255)
                                                                                                                                                   aGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCATCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGcc   >  2:2605436/1‑150 (MQ=255)
                                                                                                                                                    gATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACAGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCACTTCAGACAGCGcca  >  1:2427807/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTTTTAGGAACAAAAACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCT  >  CP009273/4571085‑4571382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑