Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 360,752 A→G 100% V331A (GTG→GCG)  lacI ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273360,7520AG96.8% 35050.7 / inf 9043V331A (GTG→GCG) lacIDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (130/163);  new base G (4165/4577);  total (4299/4744)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 9046 total aligned reads displayed.

GCTGATGCTCGGCGAAGCCAAGCCCAGCGCAGATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATA  >  CP009273/360603‑360900
                                                                                                                                                     |                                                                                                                                                    
   tgataagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCa                                                                                                                                                   <  1:1541232‑M1/104‑1 (MQ=255)
     ataagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTGGGCGCCAgg                                                                                                                                                 <  2:3549508‑M1/106‑1 (MQ=255)
        agctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACTTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGggtg                                                                                                                                              >  2:3469953‑M1/42‑150 (MQ=255)
        agctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGggtg                                                                                                                                              >  2:2277277‑M1/42‑150 (MQ=255)
              caaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGttttt                                                                                                                                        >  2:2202751‑M1/36‑150 (MQ=255)
                aaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTGGGCGCCAGGGTGGTttttct                                                                                                                                      <  1:3939288‑M1/117‑1 (MQ=255)
                aaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTGGGCGCCAGGGTGGTttttct                                                                                                                                      <  2:3408911‑M1/117‑1 (MQ=255)
                aaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttct                                                                                                                                      >  1:1385616‑M1/34‑150 (MQ=255)
                aaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttct                                                                                                                                      <  2:1257446‑M1/117‑1 (MQ=255)
                 accagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctt                                                                                                                                     >  1:3774660‑M1/33‑150 (MQ=255)
                   cagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                   <  2:1115676‑M1/120‑1 (MQ=255)
                      atcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAc                                                                                                                                <  1:1698085‑M1/123‑1 (MQ=255)
                      atcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAc                                                                                                                                <  1:1770937‑M1/123‑1 (MQ=255)
                        caattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCa                                                                                                                              >  1:47370‑M1/26‑150 (MQ=255)
                        caattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCa                                                                                                                              <  1:3626035‑M1/125‑1 (MQ=255)
                        caattcgcgctaacttacattaagtGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCa                                                                                                                              >  2:4303924‑M1/26‑150 (MQ=255)
                         aattcgcgctaacttacattaagtGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAg                                                                                                                             >  1:2178917‑M1/25‑150 (MQ=255)
                          attcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGt                                                                                                                            <  2:690385‑M1/127‑1 (MQ=255)
                           ttcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTg                                                                                                                           >  2:2353828‑M1/23‑150 (MQ=255)
                            tcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTga                                                                                                                          <  2:2430497‑M1/129‑1 (MQ=255)
                             cgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgag                                                                                                                         <  1:3857920‑M1/130‑1 (MQ=255)
                              gcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgaga                                                                                                                        <  1:635969‑M1/131‑1 (MQ=255)
                               cgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGAc                                                                                                                       >  1:1229440‑M1/19‑150 (MQ=255)
                               cgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGAc                                                                                                                       <  1:3132241‑M1/132‑1 (MQ=255)
                                gctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACg                                                                                                                      >  1:2835732‑M1/18‑150 (MQ=255)
                                  taacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACggg                                                                                                                    >  2:148692‑M1/16‑150 (MQ=255)
                                      ttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAc                                                                                                                >  2:2866623‑M1/12‑150 (MQ=255)
                                      ttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTAAACAGTGAGACGGGCAAc                                                                                                                <  1:3005217‑M1/139‑1 (MQ=255)
                                       tacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTGTTTTAACCAGTGTGACGGGCAACa                                                                                                               <  1:1457322‑M1/140‑1 (MQ=255)
                                       tacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACa                                                                                                               >  1:3548059‑M1/11‑150 (MQ=255)
                                       tacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACa                                                                                                               >  1:1157487‑M1/11‑150 (MQ=255)
                                        acattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAg                                                                                                              >  1:2925379‑M1/10‑150 (MQ=255)
                                         cattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGc                                                                                                             <  2:843824‑M1/142‑1 (MQ=255)
                                         cattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGc                                                                                                             <  2:1028965‑M1/142‑1 (MQ=255)
                                            taattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGa                                                                                                          >  2:621189‑M1/6‑150 (MQ=255)
                                             aattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAacagcggaa                                                                                                         <  2:52084‑M1/146‑5 (MQ=255)
                                             aattGCGTTGCGCTCACTGCCCGCTTACCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCCACAGCTGAt                                                                                                         <  1:161552‑M1/146‑1 (MQ=255)
                                                    ttGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCATTTCACCAGTGAGACGGGCAACAGCAGAATGCCCtt                                                                                                  >  2:165481/1‑150 (MQ=255)
                                                        gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAcc                                                                                              <  1:702234/150‑1 (MQ=255)
                                                         cTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTGTTTTCACCAGTGAGAAGGGGAACAGCTGATTTCCCTTCACCg                                                                                             >  1:423322/1‑150 (MQ=255)
                                                             cTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGATGATTGCCCTTCACCGCCTg                                                                                         <  1:782753/150‑1 (MQ=255)
                                                                   gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTg                                                                                   >  1:2430474/1‑150 (MQ=255)
                                                                       tCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCGgagag                                                                               >  2:3030826/1‑150 (MQ=255)
                                                                       tCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTgagag                                                                               >  2:3971627/1‑150 (MQ=255)
                                                                             cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGGGAAGGGCAACAGCTGATTTCCCGTCACCCCCTGGCCCTGGGAGCGTTgc                                                                         >  1:1868587/1‑150 (MQ=255)
                                                                                gAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgcagc                                                                      >  2:3254669/1‑150 (MQ=255)
                                                                                 aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCATCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgcagca                                                                     <  1:92952/150‑1 (MQ=255)
                                                                                 aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTAACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTtcagca                                                                     >  2:761800/1‑150 (MQ=255)
                                                                                  aaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCaa                                                                    <  2:4224310/150‑1 (MQ=255)
                                                                                   aCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAg                                                                   >  2:2057624/1‑150 (MQ=255)
                                                                                    ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGc                                                                  <  2:3313187/150‑1 (MQ=255)
                                                                                            gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACg                                                          <  1:3206678/150‑1 (MQ=255)
                                                                                             ccAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGc                                                         >  2:1573030/1‑150 (MQ=255)
                                                                                              cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt                                                        <  2:3761630/150‑1 (MQ=255)
                                                                                              cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt                                                        <  1:2685543/150‑1 (MQ=255)
                                                                                              cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt                                                        <  2:1896243/150‑1 (MQ=255)
                                                                                              cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCAGATTGCCCATCACCCCCAGGCCCCGAGAGAGTTGCAGCCAGCGGTCCAccca                                                        >  1:2347111/1‑147 (MQ=255)
                                                                                               aGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTg                                                       >  2:1415947/1‑150 (MQ=255)
                                                                                               aGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTg                                                       <  1:2765575/150‑1 (MQ=255)
                                                                                                gCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCAGGCCCGGAGAGAGTTGCAGCAAGCGGTCCACGCTgg                                                      >  1:2107138/1‑150 (MQ=255)
                                                                                                   gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGttt                                                   <  2:1184359/150‑1 (MQ=255)
                                                                                                    cATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGTGTATTGGGCGCCAGGGTGGTTTTTCTTTTAACCAGTGAGACGGGCCACAGCAGATTTCCCATCACCCCCAGGCCCAGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTg                                                  >  1:4168531/1‑150 (MQ=255)
                                                                                                     aTTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCAGATTGCCCATCACCGCCAGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGGTTGc                                                 >  1:1001231/1‑150 (MQ=255)
                                                                                                      ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGcc                                                <  1:2262685/150‑1 (MQ=255)
                                                                                                      ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGcc                                                <  1:859774/150‑1 (MQ=255)
                                                                                                         aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCca                                             <  2:1737501/150‑1 (MQ=255)
                                                                                                         aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCca                                             <  1:346961/150‑1 (MQ=255)
                                                                                                          tGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcag                                            <  2:1822552/150‑1 (MQ=255)
                                                                                                          tGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTAACCAGTGAGACCGGCCCCACCAGATTTCCCCTCACCCCCAGGCCCAGCGAGAGTTTCCGCCAGCGGTCCACGCAGGGTTGcccccc                                            >  1:3381416/1‑148 (MQ=255)
                                                                                                               cGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGc                                       >  2:3621113/1‑150 (MQ=255)
                                                                                                                ggCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCg                                      <  1:4007946/150‑1 (MQ=255)
                                                                                                                 gCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTAACCAGTGAGAAGGGCAACACCTGATTGCCCATCACCGCCAGGCCCCGAGAGAGTTTCAGCAACCGGTCCACGCTGGTTTGCCCCAGCAGGCGa                                     >  1:3705141/1‑150 (MQ=255)
                                                                                                                  ccAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGaa                                    <  2:3177737/150‑1 (MQ=255)
                                                                                                                      cgcgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATc                                <  1:3072298/150‑1 (MQ=255)
                                                                                                                       gcgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATcc                               <  1:2513234/150‑1 (MQ=255)
                                                                                                                        cgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCt                              <  2:4147501/150‑1 (MQ=255)
                                                                                                                        cgcgGGGAGAGGCGGTTTGAGTAGTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCt                              <  1:2594744/150‑1 (MQ=255)
                                                                                                                          cgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGt                            <  1:2035869/150‑1 (MQ=255)
                                                                                                                           ggggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGtt                           <  2:4055558/150‑1 (MQ=255)
                                                                                                                           ggggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTCAACACTGATAAGGGCAACAGCTGATTTCCCATAAACCCCAGGCCCAGCGAGAGTTTCAGCAAGCGGTACACCCTGGTTTTCCCCACCACGCGAAAATACAGtt                           >  1:4078593/1‑150 (MQ=255)
                                                                                                                                gagGCGGTTTGCGTATTGGGCGCCAGGGTGGTGTTTATTTTAACCAGTGAGAAGGGCAACAACAGTTTTCCCATAAACCCCAGGCCCAGGGGGAGTTTCAGCAAACCGTACACGCTGGTTTTCCCCACCAGGCGAAAAAACAGTTTGAtt                      >  1:3289009/1‑149 (MQ=255)
                                                                                                                                 agGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCATCACCGCCTGGCCCGGGGAGAGTTGCAGCAACCGGTCCACCCTGGTTTGCCCCAGCAGGCGAAAATACTGTTTGTtgg                     >  1:1311067/1‑150 (MQ=255)
                                                                                                                                 agGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAACACTGAGAAGGGCAACAGCTGAATTCCCATCACCCCCAGGCCCGGAGCGAGTTGCAGCAAGCGGTCCACCCTGGTTTGCCCCACCAGGCGAAAATACAGTTTGAtgg                     >  1:107649/1‑150 (MQ=255)
                                                                                                                                    cGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg                  >  2:3695678/1‑150 (MQ=255)
                                                                                                                                     ggTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATggtggt                 >  2:3897619/1‑150 (MQ=255)
                                                                                                                                      gTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCAGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt                >  2:3824548/1‑150 (MQ=255)
                                                                                                                                      gTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAACAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCGGAGAGAGTTGCAGCCAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAAACTGTTTGATGgttgtt                >  1:543630/1‑150 (MQ=255)
                                                                                                                                        ttGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTaa              <  2:448982/150‑1 (MQ=255)
                                                                                                                                          gCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcg            <  1:4313042/150‑1 (MQ=255)
                                                                                                                                           cGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg           <  2:909130/150‑1 (MQ=255)
                                                                                                                                           cGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg           >  2:2499727/1‑150 (MQ=255)
                                                                                                                                           cGTATTGGGCGCCAGGGTGGTTTTTATTTTCACCACTGAGACGGGCAACAGCTGATTTCCCATCACCCCCAGGCCCAGAGAGAGTTGCAGCAAGCGGTCCACCCTGGTTTGCCCCACCACGCGAAAATACTGTTTGATTGTGGTTAAcgg           >  1:1951904/1‑150 (MQ=255)
                                                                                                                                           cGTATTGGGCACCAGGGTGGTTTTTATTTTAACCAATGAGACGGGCAACAGCAGATTGCCCTTCACCGCCTTGCCCTGAGATAGTTGCAGCAAGCGGTCCACCCTGGTTTTCCCCAGCAGGCGAAAATCCAGTTTTAAGGTGGTTAAcgg           >  2:2771693/1‑150 (MQ=255)
                                                                                                                                            gTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggc          >  2:1970819/1‑150 (MQ=255)
                                                                                                                                               ttGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCCACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCGGTTTGAGTGTGGTTAACGGCggg       >  2:1336989/1‑150 (MQ=255)
                                                                                                                                               ttGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg       <  2:267992/150‑1 (MQ=255)
                                                                                                                                               ttGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg       <  2:2945868/150‑1 (MQ=255)
                                                                                                                                                tGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGa      <  2:2126940/150‑1 (MQ=255)
                                                                                                                                                  ggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGata    <  2:2596824/150‑1 (MQ=255)
                                                                                                                                                  ggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGata    >  2:3108036/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCTGATGCTCGGCGAAGCCAAGCCCAGCGCAGATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATA  >  CP009273/360603‑360900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑