Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 362,159 G→T 91.6% I165I (ATC→ATA)  mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273362,1590GT91.6% 953.0 / 69.1 317I165I (ATC→ATA) mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (8/19);  new base T (142/148);  total (150/167)
Fisher's exact test for biased strand distribution p-value = 6.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  CP009273/362011‑362308
                                                                                                                                                    |                                                                                                                                                     
cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTAATCACCGGGGCGGGAGGCTAACAATTCGa                                                                                                                                                      >  2:3636/1‑150 (MQ=255)
 ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTAt                                                                                                                                                     <  1:1172386/150‑1 (MQ=255)
  gCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTAtt                                                                                                                                                    <  1:4048629/150‑1 (MQ=255)
       agagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTc                                                                                                                                               <  1:4028655/150‑1 (MQ=255)
       agagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTc                                                                                                                                               >  2:3867417/1‑150 (MQ=255)
       agagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTc                                                                                                                                               <  1:2671557/150‑1 (MQ=255)
         agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCt                                                                                                                                             <  2:2055062/150‑1 (MQ=255)
          ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCtt                                                                                                                                            <  2:4219325/150‑1 (MQ=255)
           gCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTg                                                                                                                                           <  2:2213055/150‑1 (MQ=255)
            cGATATTCTACTCCTGATCCCAGCCGCGGTAGTTCTGTCCGAAACCCTCTTTGCGCGCGAGAGCCAGAATGGCTTACAGCTTTAACGGTTCCCTTTCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGc                                                                                                                                          >  2:2306790/1‑150 (MQ=255)
             gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCg                                                                                                                                         >  2:3771744/1‑150 (MQ=255)
                    tcctccTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGt                                                                                                                                  >  1:969903/1‑150 (MQ=255)
                       tcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGttac                                                                                                                               >  1:3076900/1‑148 (MQ=255)
                        cctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCg                                                                                                                              <  1:1040900/150‑1 (MQ=255)
                          tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                            >  1:3898079/1‑150 (MQ=255)
                          tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                            >  2:3367781/1‑150 (MQ=255)
                          tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCggg                                                                                                                            <  2:3904595/150‑1 (MQ=255)
                              cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaa                                                                                                                        >  2:839415/1‑150 (MQ=255)
                               ccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCaaaa                                                                                                                       <  2:1526327/150‑1 (MQ=255)
                                cAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAg                                                                                                                      >  1:3346408/1‑150 (MQ=255)
                                  gCCGCGGTAGGTCTGTCCGTAACCCTCTGTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgc                                                                                                                    <  2:2692814/150‑1 (MQ=255)
                                   ccGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgcc                                                                                                                   >  2:2439579/1‑150 (MQ=255)
                                   ccGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCATTGCGGTCTTGTTCCGGGCACAAGgcc                                                                                                                   >  2:3773724/1‑150 (MQ=255)
                                    cgcgGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgcca                                                                                                                  >  1:2984288/1‑150 (MQ=255)
                                       ggTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcc                                                                                                               >  2:1619778/1‑150 (MQ=255)
                                        gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagcca                                                                                                              <  2:845352/150‑1 (MQ=255)
                                          aGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAgg                                                                                                            >  2:1824055/1‑150 (MQ=255)
                                            ttCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTc                                                                                                          >  2:3333668/1‑150 (MQ=255)
                                             tCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCa                                                                                                         <  1:2581018/150‑1 (MQ=255)
                                              cTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAg                                                                                                        <  1:4272288/150‑1 (MQ=255)
                                               tGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAgg                                                                                                       >  2:3147245/1‑150 (MQ=255)
                                                gTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGc                                                                                                      >  2:4259642/1‑150 (MQ=255)
                                                gTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGc                                                                                                      <  1:633870/150‑1 (MQ=255)
                                                  ccGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGccc                                                                                                    <  2:3596820/150‑1 (MQ=255)
                                                    gTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGa                                                                                                  <  2:1601244/150‑1 (MQ=255)
                                                       aCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGc                                                                                               >  2:1778080/1‑150 (MQ=255)
                                                       aCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGc                                                                                               <  1:1903422/150‑1 (MQ=255)
                                                       aCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAAGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGc                                                                                               <  2:2705290/150‑1 (MQ=255)
                                                          ctctTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGt                                                                                            >  1:1221019/1‑150 (MQ=255)
                                                              ttGCGCGCGCGCGCCAGAAGGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTc                                                                                        <  2:3531746/150‑1 (MQ=255)
                                                               tGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCa                                                                                       <  1:2138410/150‑1 (MQ=255)
                                                               tGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCa                                                                                       >  2:4140889/1‑150 (MQ=255)
                                                               tGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCa                                                                                       <  1:4310537/150‑1 (MQ=255)
                                                                gcgcgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAg                                                                                      <  2:2617858/150‑1 (MQ=255)
                                                                  gcgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGACTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGaa                                                                                    <  2:1121601/150‑1 (MQ=255)
                                                                  gcgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGACTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGaa                                                                                    <  2:2925603/150‑1 (MQ=255)
                                                                   cgcgcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAg                                                                                   <  1:3015200/150‑1 (MQ=255)
                                                                   cgcgcgcgcCAGAAGGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAg                                                                                   <  2:1359634/150‑1 (MQ=255)
                                                                      gcgcgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCgg                                                                                <  1:3883326/150‑1 (MQ=255)
                                                                         cgcCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCaa                                                                             >  2:959038/1‑150 (MQ=255)
                                                                           ccAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAAc                                                                           >  1:4167960/1‑150 (MQ=255)
                                                                            cAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACg                                                                          >  1:2328424/1‑150 (MQ=255)
                                                                              gAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTc                                                                        >  1:3167461/1‑150 (MQ=255)
                                                                                  ggCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGccc                                                                    <  1:4250643/150‑1 (MQ=255)
                                                                                   gCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCg                                                                   <  1:135793/150‑1 (MQ=255)
                                                                                      tCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGAcc                                                                <  1:1444989/150‑1 (MQ=255)
                                                                                       ccAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCa                                                               >  2:1006076/1‑150 (MQ=255)
                                                                                        cAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAt                                                              <  1:1509276/150‑1 (MQ=255)
                                                                                         aGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCAtt                                                             >  1:2773838/1‑150 (MQ=255)
                                                                                          gCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTg                                                            <  1:2657407/150‑1 (MQ=255)
                                                                                            tttAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGcc                                                          >  2:832263/1‑150 (MQ=255)
                                                                                             ttAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGccc                                                         >  2:889731/1‑150 (MQ=255)
                                                                                             ttAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGccc                                                         >  2:510738/1‑150 (MQ=255)
                                                                                               aaCGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCgg                                                       >  1:551273/1‑150 (MQ=255)
                                                                                                aCGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGt                                                      <  1:3749830/150‑1 (MQ=255)
                                                                                                 cGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCTGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTg                                                     <  2:1989213/150‑1 (MQ=255)
                                                                                                 cGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTg                                                     <  2:4329314/150‑1 (MQ=255)
                                                                                                 cGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTg                                                     >  1:150837/1‑150 (MQ=255)
                                                                                                    gtCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGaaa                                                  <  2:523151/149‑1 (MQ=255)
                                                                                                     tCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAg                                                 >  1:521337/1‑150 (MQ=255)
                                                                                                     tCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAg                                                 <  1:479409/150‑1 (MQ=255)
                                                                                                     tCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAg                                                 >  2:305249/1‑150 (MQ=255)
                                                                                                     tCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAg                                                 <  2:34799/150‑1 (MQ=255)
                                                                                                        cGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGAt                                              <  1:2786243/150‑1 (MQ=255)
                                                                                                         gTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATa                                             <  1:3447997/150‑1 (MQ=255)
                                                                                                             cAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCg                                         >  1:2389729/1‑150 (MQ=255)
                                                                                                              aGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCgg                                        <  1:2633979/150‑1 (MQ=255)
                                                                                                               gTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGc                                       <  1:1067317/150‑1 (MQ=255)
                                                                                                               gTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGc                                       <  1:3251850/150‑1 (MQ=255)
                                                                                                                ttGATCGTCATCACCGGGGCGGGAGGCTAACATTTATATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                      <  2:539475/150‑1 (MQ=255)
                                                                                                                ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                      <  1:2267721/150‑1 (MQ=255)
                                                                                                                ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                      <  1:1628272/150‑1 (MQ=255)
                                                                                                                ttGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCt                                      >  2:3905156/1‑150 (MQ=255)
                                                                                                                    tAGTCATCACCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGaaa                                  >  1:2236574/1‑150 (MQ=255)
                                                                                                                            aCCGGGGCGGGAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGt                          >  2:2139297/1‑150 (MQ=255)
                                                                                                                                 ggCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGttt                     <  2:3967308/150‑1 (MQ=255)
                                                                                                                                 ggCGGGAGGCTAACATGTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGttt                     <  1:8938/150‑1 (MQ=255)
                                                                                                                                  gCGGGAGGCTAACATTTCTATTAAGTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTc                    <  1:2437958/150‑1 (MQ=255)
                                                                                                                                   cGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcg                   <  1:3636/150‑1 (MQ=255)
                                                                                                                                    gggAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTcgc                  >  2:1287872/1‑150 (MQ=255)
                                                                                                                                      gAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGt                >  1:2501824/1‑150 (MQ=255)
                                                                                                                                      gAGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGt                >  1:2723158/1‑150 (MQ=255)
                                                                                                                                      gAGGCTAACATGTCGATTCATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATCAGCGGCCGAAACGGAGAGTGGTTTCGCGt                <  2:2529763/150‑1 (MQ=255)
                                                                                                                                       aGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTa               <  1:2870216/150‑1 (MQ=255)
                                                                                                                                       aGGCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTa               <  1:1778080/150‑1 (MQ=255)
                                                                                                                                         gCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTacc             >  1:2826431/1‑150 (MQ=255)
                                                                                                                                         gCTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTacc             <  2:2852041/150‑1 (MQ=255)
                                                                                                                                          cTAACATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTacca            >  2:1739771/1‑150 (MQ=255)
                                                                                                                                             aCATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTAccacca         <  2:238525/150‑1 (MQ=255)
                                                                                                                                              cATTTCTATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTAccaccaa        >  1:1128319/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATC  >  CP009273/362011‑362308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑