Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 402,340 G→T 93.8% V160V (GTG→GTT)  aroL → shikimate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273402,3400GT93.8% 726.2 / 34.5 226V160V (GTG→GTT) aroLshikimate kinase II
Reads supporting (aligned to +/- strand):  ref base G (6/8);  new base T (107/105);  total (113/113)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 226 total aligned reads displayed.

AGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAA  >  CP009273/402196‑402486
                                                                                                                                                |                                                                                                                                                  
  cTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTg                                                                                                                                             <  1:2424585/150‑1 (MQ=255)
   tGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGa                                                                                                                                            <  2:2253651/150‑1 (MQ=255)
   tGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGa                                                                                                                                            >  2:1498251/1‑150 (MQ=255)
      aCCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAAt                                                                                                                                         <  2:1202121/150‑1 (MQ=255)
         ggAAGAAGATTTACGGCCAACCTAAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCg                                                                                                                                      <  1:1160650/150‑1 (MQ=255)
          gaagaaGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGc                                                                                                                                     >  1:1971585/1‑150 (MQ=255)
           aagaagATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCa                                                                                                                                    >  2:1004637/1‑150 (MQ=255)
            ggaagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAg                                                                                                                                   >  1:357473/2‑150 (MQ=255)
             gaagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgc                                                                                                                                  >  1:4147375/1‑150 (MQ=255)
             gaagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgc                                                                                                                                  <  2:2594509/150‑1 (MQ=255)
             gaagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgc                                                                                                                                  <  1:2727902/150‑1 (MQ=255)
             gaagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgc                                                                                                                                  <  1:2720890/150‑1 (MQ=255)
             gaagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgc                                                                                                                                  <  1:2645785/150‑1 (MQ=255)
                   ttACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTg                                                                                                                            <  2:4111052/150‑1 (MQ=255)
                    tACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTgg                                                                                                                           <  2:629106/150‑1 (MQ=255)
                         ccAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAg                                                                                                                      <  1:3272386/150‑1 (MQ=255)
                          cAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGa                                                                                                                     <  2:39677/150‑1 (MQ=255)
                            aCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACg                                                                                                                   >  1:530863/1‑150 (MQ=255)
                            aCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACg                                                                                                                   >  1:530532/1‑150 (MQ=255)
                             ccTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGa                                                                                                                  >  2:1398692/1‑150 (MQ=255)
                                tAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                               >  1:713972/1‑150 (MQ=255)
                                 aaCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAGCac                                                                                                              >  1:1520348/1‑149 (MQ=255)
                                     ggAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgt                                                                                                          >  1:595018/1‑150 (MQ=255)
                                     ggAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgt                                                                                                          <  1:2662416/150‑1 (MQ=255)
                                      gAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgtt                                                                                                         >  1:4349390/1‑150 (MQ=255)
                                       aaaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgttg                                                                                                        <  1:3100048/150‑1 (MQ=255)
                                        aaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGa                                                                                                       >  1:2313841/1‑150 (MQ=255)
                                              cTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCg                                                                                                 >  2:1171512/1‑150 (MQ=255)
                                               tGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGa                                                                                                >  2:1321684/1‑150 (MQ=255)
                                               tGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGa                                                                                                >  1:3619878/1‑150 (MQ=255)
                                               tGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGa                                                                                                >  1:3109655/1‑150 (MQ=255)
                                               tGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGa                                                                                                >  1:3370247/1‑150 (MQ=255)
                                                gAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAg                                                                                               <  1:3368598/150‑1 (MQ=255)
                                                  gCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgcg                                                                                             <  1:3707104/150‑1 (MQ=255)
                                                      agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                         <  2:3083826/150‑1 (MQ=255)
                                                      agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                         >  2:4291962/1‑150 (MQ=255)
                                                       gaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCtat                                                                                        <  2:766925/150‑1 (MQ=255)
                                                        aagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCtata                                                                                       <  1:98638/150‑1 (MQ=255)
                                                         agTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATAc                                                                                      <  2:373703/150‑1 (MQ=255)
                                                         agTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATAc                                                                                      >  2:3404012/1‑150 (MQ=255)
                                                            tCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                   <  1:1631745/150‑1 (MQ=255)
                                                             cAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTaa                                                                                  >  2:3699575/1‑150 (MQ=255)
                                                             cAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTaa                                                                                  <  1:4095042/150‑1 (MQ=255)
                                                              aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAc                                                                                 >  1:3380084/1‑150 (MQ=255)
                                                              aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAc                                                                                 <  1:2441431/150‑1 (MQ=255)
                                                               ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACg                                                                                <  1:198713/150‑1 (MQ=255)
                                                               ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACg                                                                                <  1:3063703/150‑1 (MQ=255)
                                                               ggAAGTGCTGGAAGAACGCGATGAGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACg                                                                                >  2:1886948/1‑150 (MQ=255)
                                                                    tGCTGGAAGAACTCGATGCGCTATTTCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAGCAATTGTTGATTTTCGAGCGAATATACTTAACGTTCAt                                                                           <  2:2862934/150‑1 (MQ=255)
                                                                      cTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATcc                                                                         >  1:2864524/1‑150 (MQ=255)
                                                                         gaagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGt                                                                      >  2:3071271/1‑150 (MQ=255)
                                                                          aagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTg                                                                     <  2:491306/150‑1 (MQ=255)
                                                                           agaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGa                                                                    <  2:1341568/150‑1 (MQ=255)
                                                                              aCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAAt                                                                 >  2:2041043/1‑150 (MQ=255)
                                                                                gcgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATaa                                                               <  2:3791757/150‑1 (MQ=255)
                                                                                 cgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAg                                                              <  1:1292275/150‑1 (MQ=255)
                                                                                  gATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAgg                                                             >  2:1387549/1‑150 (MQ=255)
                                                                                  gATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAgg                                                             <  2:3853948/150‑1 (MQ=255)
                                                                                   aTGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGga                                                            >  1:386051/1‑150 (MQ=255)
                                                                                        cTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAAc                                                       <  1:1950394/150‑1 (MQ=255)
                                                                                         tataTCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACg                                                      >  2:4188243/1‑150 (MQ=255)
                                                                                          atatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGa                                                     >  2:3553606/1‑150 (MQ=255)
                                                                                          atatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGa                                                     >  2:3208945/1‑150 (MQ=255)
                                                                                          atatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGa                                                     >  2:2366441/1‑150 (MQ=255)
                                                                                           tatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGAt                                                    <  2:357473/150‑1 (MQ=255)
                                                                                           tatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGAt                                                    >  1:4250635/1‑150 (MQ=255)
                                                                                           tatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGAt                                                    >  1:1778877/1‑150 (MQ=255)
                                                                                            atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATg                                                   <  1:2008398/150‑1 (MQ=255)
                                                                                              cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGcc                                                 >  1:3838713/1‑150 (MQ=255)
                                                                                                cgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCaa                                               >  1:2527798/1‑150 (MQ=255)
                                                                                                   aGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGa                                            >  1:2533450/1‑150 (MQ=255)
                                                                                                    gTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaa                                           >  2:1732158/1‑150 (MQ=255)
                                                                                                    gTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaa                                           <  1:3989215/150‑1 (MQ=255)
                                                                                                     ttGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaaa                                          <  1:582688/150‑1 (MQ=255)
                                                                                                         gcATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGc                                      >  1:1906097/1‑150 (MQ=255)
                                                                                                           atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCt                                    >  2:1621799/1‑150 (MQ=255)
                                                                                                             attatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTa                                  <  1:2279641/150‑1 (MQ=255)
                                                                                                             attatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTa                                  >  1:2342466/1‑150 (MQ=255)
                                                                                                              ttatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAt                                 >  2:3605388/1‑150 (MQ=255)
                                                                                                                atcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATcc                               >  1:274870/1‑150 (MQ=255)
                                                                                                                atcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATcc                               <  1:1741324/150‑1 (MQ=255)
                                                                                                                atcatcGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATcc                               >  1:1645484/1‑150 (MQ=255)
                                                                                                                 tcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCt                              >  1:787750/1‑150 (MQ=255)
                                                                                                                   atcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCg                            >  1:1130958/1‑150 (MQ=255)
                                                                                                                    tcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGt                           >  1:1753595/1‑150 (MQ=255)
                                                                                                                          caacaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCa                     <  1:4214795/150‑1 (MQ=255)
                                                                                                                          caacaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCa                     >  2:2940959/1‑150 (MQ=255)
                                                                                                                           aacaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCat                    >  1:3461349/1‑150 (MQ=255)
                                                                                                                           aacaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCat                    >  1:1388170/1‑150 (MQ=255)
                                                                                                                            acaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCata                   <  2:1690163/150‑1 (MQ=255)
                                                                                                                             caaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatat                  <  1:3791701/150‑1 (MQ=255)
                                                                                                                              aaaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatata                 >  1:3719880/1‑150 (MQ=255)
                                                                                                                               aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatatat                >  2:1449726/1‑150 (MQ=255)
                                                                                                                               aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatatat                >  2:1448741/1‑150 (MQ=255)
                                                                                                                                    aCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGa           >  1:1694114/1‑150 (MQ=255)
                                                                                                                                     cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAc          <  2:1906853/150‑1 (MQ=255)
                                                                                                                                       cagccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGa        <  1:215616/150‑1 (MQ=255)
                                                                                                                                       cagccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGa        <  1:215035/150‑1 (MQ=255)
                                                                                                                                            agGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGaa   >  1:3123338/1‑150 (MQ=255)
                                                                                                                                             ggTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGaaa  >  1:3548251/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
AGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAA  >  CP009273/402196‑402486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑