| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 450,290 | G→A | 100% | intergenic (+45/‑299) | bolA → / → tig | stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 450,290 | 0 | G | A | 92.1% | 653.0 / 33.7 | 202 | intergenic (+45/‑299) | bolA/tig | stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor) |
| Reads supporting (aligned to +/- strand): ref base G (7/9); new base A (80/106); total (87/115) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
Only 100 of 203 total aligned reads displayed.
TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG > CP009273/450143‑450436 | tCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt < 2:941252/150‑1 (MQ=255)tCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt > 2:3604342/1‑150 (MQ=255) cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt < 1:4615736/150‑1 (MQ=255) cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt < 1:1653530/150‑1 (MQ=255) aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGTAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt < 1:3499888/150‑1 (MQ=255) aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt > 1:3725863/1‑150 (MQ=255) cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt < 2:4106413/150‑1 (MQ=255) gcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCTAAAAATTTTATg > 1:3299533/1‑150 (MQ=255) ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATTTTGCTAAAAATTTTATGaaaaa > 2:2970491/1‑150 (MQ=255) ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa > 1:2023939/1‑150 (MQ=255) ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa > 1:2024142/1‑150 (MQ=255) tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc < 2:4769517/150‑1 (MQ=255) tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTGTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc < 2:4478301/150‑1 (MQ=255) tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACgg < 2:4314253/150‑1 (MQ=255) gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc > 1:4412604/1‑150 (MQ=255) cATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc > 1:2515875/1‑150 (MQ=255) aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCt < 2:456725/150‑1 (MQ=255) aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCt < 2:1230726/150‑1 (MQ=255) aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt > 1:1534996/1‑150 (MQ=255) aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt > 1:67758/1‑150 (MQ=255) aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt > 1:4071828/1‑150 (MQ=255) aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt > 1:3945525/1‑150 (MQ=255) aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGc > 1:3680096/1‑150 (MQ=255) cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt > 1:1962179/1‑150 (MQ=255) tATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt > 2:2611897/1‑150 (MQ=255) aTTATGGAGTGGGACGGGTTGCAGGACACCGTATTTGCCTCTCCTCCCTGTCGTGGAGCCGGAAGCATCGCTTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTTTGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCgttt < 1:846968/150‑1 (MQ=255) aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCggtt > 2:3819734/1‑150 (MQ=255) aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCggtt > 1:2647455/1‑150 (MQ=255) aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt < 1:1834217/150‑1 (MQ=255) tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg > 2:1518563/1‑150 (MQ=255) aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg < 2:2167975/150‑1 (MQ=255) aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg < 2:1224173/150‑1 (MQ=255) gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTtttgttttgt < 2:2100379/150‑1 (MQ=255) tGGGAAGGGTGGCAGGACACCGTCTGGGCCGCTCCGCCCTGTCGCGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACGGTCGGCGCTTTTCCAGCAGGTTGCTAAAAATTTTATGACAAACGGCCTGCGGGCCGTTTTGTTTTGTc < 2:4723970/150‑1 (MQ=255) gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTtgtgttttgtgt > 1:723497/1‑148 (MQ=255) gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCt > 2:947162/1‑150 (MQ=255) aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGa > 2:2620470/1‑150 (MQ=255) ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTTGAttt > 2:637839/1‑150 (MQ=255) gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt < 1:2899836/150‑1 (MQ=255) tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTGGTGGAGCAGGAAGCATCGAGTAAAAACGCATTTGCAACGGTCGGCGATTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc < 1:4478009/150‑1 (MQ=255) tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc > 2:2786348/1‑150 (MQ=255) tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTgc > 2:394702/1‑150 (MQ=255) cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt < 2:3010696/150‑1 (MQ=255) cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGATTTTGCGCttttt > 2:4236289/1‑150 (MQ=255) acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg < 2:3478039/150‑1 (MQ=255) ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGc > 2:1944203/1‑150 (MQ=255) ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTTCGCTTTTTGc > 2:2455632/1‑150 (MQ=255) tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt < 1:1982955/150‑1 (MQ=255) tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt < 2:4443472/150‑1 (MQ=255) tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt < 2:3592977/150‑1 (MQ=255) tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt < 2:2040276/150‑1 (MQ=255) tcgcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATGTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg < 1:4458186/147‑1 (MQ=255) cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg < 2:1427624/150‑1 (MQ=255) cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg < 1:4733996/150‑1 (MQ=255) cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg < 1:4731786/150‑1 (MQ=255) ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTATGGATTTTGCGCTTTTTTCCCAGCAATCAGAcca > 2:3275319/1‑148 (MQ=255) ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑anaa < 2:25567/150‑1 (MQ=255) ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGTGGATTTTTCGGTTTTTGCCCAGCAAACACACG‑aaaa > 1:3902510/1‑150 (MQ=255) ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTTGTTTTTGGGGGTTTTGGACACCCATCACACG‑aaaa > 1:3828294/1‑150 (MQ=255) gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTg < 1:2874971/150‑1 (MQ=255) tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg < 2:64850/150‑1 (MQ=255) tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg < 1:2897933/150‑1 (MQ=255) tGGAACAGGAAGCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCTGTTTGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg < 1:589647/150‑1 (MQ=255) gAGCAGGAAGCATCGCATAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTGCGCTTTTTGCCCAGCCTTAAGACG‑AAAAATGCCCggg > 1:3049896/1‑150 (MQ=255) tggAAGCATCGCTTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCATTTTGTTGCTAAAATTTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt < 1:4014447/149‑1 (MQ=255) agtaaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt < 1:4278957/147‑1 (MQ=255) aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTTCGGATTTTTACCAGCAATAAGACG‑AAAAATTCCCggggaat > 1:4345199/1‑146 (MQ=255) aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt < 1:2161800/150‑1 (MQ=255) aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt < 1:2161669/150‑1 (MQ=255) aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTGGGGTTTTTTCCCAGCATTCAGACG‑AAAAATTCCCGGGAATtgtg > 1:3971626/1‑150 (MQ=255) aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtgtg < 1:2786348/150‑1 (MQ=255) aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTGTGTGGTTTTTGGGCTTTTTTCACAGCAATGAGACG‑CAAATTGCCCGGGACTtgtt > 2:2138961/1‑149 (MQ=255) aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGaaaa < 2:3521248/150‑1 (MQ=255) gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTAAGACG‑AAAATTTCCCGGGAAATGTGTAAAAAt > 2:2267352/1‑150 (MQ=255) cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTTGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATa < 2:464160/150‑1 (MQ=255) gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAc < 2:3725863/150‑1 (MQ=255) aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc < 1:4659885/150‑1 (MQ=255) aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgcg < 1:2402124/150‑1 (MQ=255) aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATGGATTTTTGGCTTTTTGGCCACCAATAAGAAG‑AAAAATGCCCGGGCAATGTGGAAAAAAAagcg > 1:4311329/1‑150 (MQ=255) aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACa < 2:3469260/150‑1 (MQ=255) aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACa < 1:3181027/150‑1 (MQ=255) gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCGGGATTTTTGGCGTTTTTCCCAGCCCTCAGACG‑AAAATTGGCCGGGAATGTGGTAAAAATAACCGACaac > 1:1887135/1‑148 (MQ=255) cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTTGATTTTTGGCATTTTGGCCAGCCATAAGGCGAAAAATTGCCC‑GGAATTGTGAAAAAATACGCGACcgcg > 2:63575/1‑150 (MQ=255) gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa < 2:4468038/150‑1 (MQ=255) cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGGTTTGTTTTTTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGTCG‑AAAAATGCCCGGGACTTGTTAAAAAATACGCGCCCGCGCGCAAt > 2:3889014/1‑150 (MQ=255) aagtGTCGGCGCTTTTCCAGTATTTTGCTAAAGATTTTATGAAAAACGGCGTGCGGGCCTTTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCTTTCAGACG‑AAAATTGCCCGTGAATTGTGAAAAAATACGCGACAGCGCGCAATa < 1:2713366/147‑1 (MQ=255) aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa < 2:470767/150‑1 (MQ=255) aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTTTGTTGGTTTTTGGCTTTTTGTCCCGCAATAACAAGAAAAAATCCCCGGGAATT‑TGTAAAAAAAAGCGACACCGCCCaaaa > 1:4175596/1‑148 (MQ=255) aaCTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa < 1:3148869/150‑1 (MQ=255) cTGTCGGCGCTTTTCCATTTTGTTGCTAAAATTTTTAGCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc < 1:524236/150‑1 (MQ=255) cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc < 2:1882334/150‑1 (MQ=255) gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct < 2:1710488/150‑1 (MQ=255) tttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCAATCAGACG‑AAAAATTCCCGGGAATTTTGAAAAAATAAGCGACAGCGCGCAATAAACGTTCTCGACt > 2:4364701/1‑150 (MQ=255) tCCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa < 2:2484384/150‑1 (MQ=255) cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa < 2:1383743/150‑1 (MQ=255) aGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa < 2:4514366/150‑1 (MQ=255) gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa < 1:105151/150‑1 (MQ=255) gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTTAGCTTTTTGCCCCGCATTCAGACG‑AAAATTGCCCGGGGATTGTGAAAAAATAAGAGACAGCGCGCAATAAACGGTCTAGACTCCAAAAAgag > 2:3392552/1‑148 (MQ=255) cTAAAAATTTCATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc < 2:3298205/150‑1 (MQ=255) tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTGGTTGATTTTTAGCTTTTTTCCCAGCAGTCAGACG‑AAAAATGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc > 2:4194858/1‑150 (MQ=255) | TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG > CP009273/450143‑450436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |