Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 486,912 C→A 26.8% I15I (ATC→ATA)  apt → adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273486,9120CA26.8% 485.2 / 163.6 223I15I (ATC→ATA) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (73/90);  new base A (30/30);  total (103/120)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 226 total aligned reads displayed.

CGTTTTCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCGGCACCGAAGC  >  CP009273/486765‑487061
                                                                                                                                                   |                                                                                                                                                     
cGTTTTCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCaa                                                                                                                                                     <  2:1962403/150‑1 (MQ=255)
 gTTTTCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTCTCTCAAAAATAGCATCaaa                                                                                                                                                    >  2:2052408/1‑150 (MQ=255)
   tttCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAg                                                                                                                                                  >  1:901159/1‑150 (MQ=255)
    ttCGAGCACAGGCGCTGGCGGTCAAAGTTTATACAACTTTTACTTTTTATACGTTTAAATCGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGc                                                                                                                                                 <  2:3582454/150‑1 (MQ=255)
     tCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCa                                                                                                                                                <  2:2240343/150‑1 (MQ=255)
     tCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCa                                                                                                                                                <  2:1986454/150‑1 (MQ=255)
      cGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCAt                                                                                                                                               >  2:532038/1‑150 (MQ=255)
       gAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCAtt                                                                                                                                              >  2:4703270/1‑150 (MQ=255)
       gAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCAtt                                                                                                                                              <  2:3541095/150‑1 (MQ=255)
        aGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTc                                                                                                                                             <  1:377865/150‑1 (MQ=255)
        aGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTc                                                                                                                                             >  1:518281/1‑150 (MQ=255)
        aGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGAATTc                                                                                                                                             >  2:375297/1‑150 (MQ=255)
          cacaGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAg                                                                                                                                           >  1:1917945/1‑150 (MQ=255)
            caggcgcaggctGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGa                                                                                                                                         <  2:2449641/150‑1 (MQ=255)
            caggcgcaggcgGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGa                                                                                                                                         <  2:3503402/150‑1 (MQ=255)
            caggcgcaggcgGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGa                                                                                                                                         <  1:2420921/150‑1 (MQ=255)
             aggcgcaggcgGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGAc                                                                                                                                        >  2:2835865/1‑150 (MQ=255)
             aggcgcaggcgGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGAc                                                                                                                                        <  1:1815780/150‑1 (MQ=255)
               gcgcaggcgGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTa                                                                                                                                      <  2:2573719/150‑1 (MQ=255)
                  caggcgGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTAccc                                                                                                                                   <  1:4600825/150‑1 (MQ=255)
                    ggcgGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCaa                                                                                                                                 >  2:3737966/1‑150 (MQ=255)
                          cAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCg                                                                                                                           >  2:3224103/1‑150 (MQ=255)
                               gTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCAtt                                                                                                                      <  2:1135811/150‑1 (MQ=255)
                                  aaaCAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCtt                                                                                                                   >  1:205464/1‑150 (MQ=255)
                                   aacaacTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCttt                                                                                                                  <  2:846969/150‑1 (MQ=255)
                                   aacaacTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCttt                                                                                                                  <  2:1617250/150‑1 (MQ=255)
                                     catcTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTc                                                                                                                <  2:79046/147‑1 (MQ=255)
                                     caacTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTc                                                                                                                <  2:697296/150‑1 (MQ=255)
                                      aacTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTcc                                                                                                               >  2:4515067/1‑150 (MQ=255)
                                           ttACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGAt                                                                                                          <  1:4046626/150‑1 (MQ=255)
                                           ttACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGAt                                                                                                          <  2:4673453/150‑1 (MQ=255)
                                             aCTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGt                                                                                                        >  2:207737/1‑150 (MQ=255)
                                              cTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTc                                                                                                       >  1:2843736/1‑150 (MQ=255)
                                              cTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTc                                                                                                       <  2:1298645/150‑1 (MQ=255)
                                                 ttGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCAcc                                                                                                    <  2:2532540/150‑1 (MQ=255)
                                                  tGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCa                                                                                                   <  2:1532238/150‑1 (MQ=255)
                                                  tGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCa                                                                                                   <  1:338840/150‑1 (MQ=255)
                                                    aTACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGc                                                                                                 >  2:2633104/1‑150 (MQ=255)
                                                     tACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCt                                                                                                <  2:735796/150‑1 (MQ=255)
                                                     tACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCt                                                                                                <  2:4651748/150‑1 (MQ=255)
                                                         tttAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACt                                                                                            <  1:618182/150‑1 (MQ=255)
                                                         tttAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACt                                                                                            <  1:653544/150‑1 (MQ=255)
                                                            aaaaCGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGa                                                                                         >  2:2158158/1‑150 (MQ=255)
                                                            aaaaCGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGa                                                                                         >  1:3030571/1‑150 (MQ=255)
                                                             aaaCGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGaa                                                                                        <  2:1447053/150‑1 (MQ=255)
                                                             aaaCGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGaa                                                                                        >  1:1497231/1‑150 (MQ=255)
                                                                cgcgCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGAc                                                                                     <  1:1714518/150‑1 (MQ=255)
                                                                   gcCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCg                                                                                  >  1:606406/1‑150 (MQ=255)
                                                                     cGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGaa                                                                                >  2:1577404/1‑150 (MQ=255)
                                                                       tGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAg                                                                              <  1:2418944/150‑1 (MQ=255)
                                                                          gTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCtt                                                                           <  1:3610285/150‑1 (MQ=255)
                                                                            accaccGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTAc                                                                         <  2:3657227/150‑1 (MQ=255)
                                                                             ccaccGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACg                                                                        >  2:4422157/1‑150 (MQ=255)
                                                                              caccGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGc                                                                       >  1:3148453/1‑150 (MQ=255)
                                                                               accGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGct                                                                      >  2:4439966/1‑150 (MQ=255)
                                                                                 cGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGctct                                                                    <  2:315692/150‑1 (MQ=255)
                                                                                 cGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGctct                                                                    <  1:2182528/150‑1 (MQ=255)
                                                                                   tAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCa                                                                  <  2:4698261/150‑1 (MQ=255)
                                                                                   tAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCa                                                                  >  1:266257/1‑150 (MQ=255)
                                                                                    aaCAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAg                                                                 <  1:1242734/150‑1 (MQ=255)
                                                                                          cAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGa                                                           >  2:188471/1‑150 (MQ=255)
                                                                                           aGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGAc                                                          <  2:2334936/150‑1 (MQ=255)
                                                                                           aGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGAc                                                          >  2:2181584/1‑150 (MQ=255)
                                                                                             gCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACtt                                                        <  1:1789722/150‑1 (MQ=255)
                                                                                              cATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTg                                                       <  2:3872684/150‑1 (MQ=255)
                                                                                              cATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTg                                                       <  2:4098621/150‑1 (MQ=255)
                                                                                                 acacTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCGAAAATAGCATAAAAAGCAATCAGGACTACCCAAAAACCGGCATTCTTTTACGAGATGACACCAGCTTACAGTAAGACCCGAAAGCTTACGATCACAGAATCGACTAGCTg                                                    >  2:790204/1‑150 (MQ=255)
                                                                                                 acacTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTg                                                    <  2:3954657/150‑1 (MQ=255)
                                                                                                   acTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGt                                                  >  1:211445/1‑150 (MQ=255)
                                                                                                      tATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGa                                               >  1:2370173/1‑150 (MQ=255)
                                                                                                        tGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGc                                             <  2:3510189/150‑1 (MQ=255)
                                                                                                         gACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCg                                            <  2:1516248/150‑1 (MQ=255)
                                                                                                          aCCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGt                                           >  2:368146/1‑150 (MQ=255)
                                                                                                           ccGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGtt                                          <  1:4550828/150‑1 (MQ=255)
                                                                                                            cgcgACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTa                                         >  2:2900103/1‑150 (MQ=255)
                                                                                                            cgcgACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTa                                         <  2:1515415/150‑1 (MQ=255)
                                                                                                                 cTGCACAGCAGCTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACaaaa                                    >  1:3271966/1‑150 (MQ=255)
                                                                                                                  tGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACaaaaa                                   >  2:2472247/1‑150 (MQ=255)
                                                                                                                     acaGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGc                                <  2:2657797/150‑1 (MQ=255)
                                                                                                                     acaGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGc                                <  2:2309802/150‑1 (MQ=255)
                                                                                                                           gcTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCAt                          <  2:2370173/150‑1 (MQ=255)
                                                                                                                           gcTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCAt                          >  1:1021127/1‑150 (MQ=255)
                                                                                                                            cTTGAGTATCTCAAAAATAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCAtt                         >  1:2503487/1‑150 (MQ=255)
                                                                                                                             ttGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTa                        >  2:1023529/1‑150 (MQ=255)
                                                                                                                             ttGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTa                        >  2:1020954/1‑150 (MQ=255)
                                                                                                                              tGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTAc                       <  2:2033897/150‑1 (MQ=255)
                                                                                                                              tGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTAc                       <  2:1917945/150‑1 (MQ=255)
                                                                                                                              tGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTAc                       >  2:178045/1‑150 (MQ=255)
                                                                                                                              tGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTAc                       <  2:1735027/150‑1 (MQ=255)
                                                                                                                              tGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTAc                       >  2:1606513/1‑150 (MQ=255)
                                                                                                                                 gTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCaa                    <  2:479599/150‑1 (MQ=255)
                                                                                                                                    tctcAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGt                 >  1:3504455/1‑150 (MQ=255)
                                                                                                                                        aaaaaTAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTc             <  2:4264102/150‑1 (MQ=255)
                                                                                                                                        aaaaaTAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTc             >  2:3606223/1‑150 (MQ=255)
                                                                                                                                         aaaaTAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCg            <  1:4191260/150‑1 (MQ=255)
                                                                                                                                         aaaaTAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCg            >  1:1760395/1‑150 (MQ=255)
                                                                                                                                         aaaaTAGCATAAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCg            >  1:2120995/1‑150 (MQ=255)
                                                                                                                                            aTAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCGGCa         >  1:1406914/1‑150 (MQ=255)
                                                                                                                                              aGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCGGCAcc       >  1:356454/1‑150 (MQ=255)
                                                                                                                                                   cAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCGGCACCGAAgc  >  2:3361027/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGTTTTCGAGCACAGGCGCAGGCGGTCAAAGGTTAAACAACTGTTACTTTTGATACGTTTAAAACGCGCCGTGAGTACCACCGTAACAAGCAGGCATACACTTATGACCGCGACTGCACAGCAGCTTGAGTATCTCAAAAATAGCATCAAAAGCATTCAGGACTACCCAAAACCCGGCATTCTTTTCCGCGATGTCACCAGCTTACTGGAAGACCCGAAAGCTTACGCTCTCAGCATCGACTTGCTGGTTGAGCGTTACAAAAATGCGGGCATTACCAAAGTTGTCGGCACCGAAGC  >  CP009273/486765‑487061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑