Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 623,935 C→A 6.6% I262I (ATC→ATA)  entB → isochorismatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273623,9350CA 6.6% 1235.4 / 40.4 324I262I (ATC→ATA) entBisochorismatase
Reads supporting (aligned to +/- strand):  ref base C (121/180);  new base A (10/12);  total (131/193)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

GCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTT  >  CP009273/623786‑624084
                                                                                                                                                     |                                                                                                                                                     
gCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATc                                                                                                                                                       <  1:4226330/150‑1 (MQ=255)
 ccAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCg                                                                                                                                                      <  1:34329/150‑1 (MQ=255)
  cAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGa                                                                                                                                                     >  2:3400229/1‑150 (MQ=255)
   aGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGAc                                                                                                                                                    >  2:4661094/1‑150 (MQ=255)
    gCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACt                                                                                                                                                   <  2:3245828/150‑1 (MQ=255)
     cAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACtt                                                                                                                                                  >  2:1225546/1‑150 (MQ=255)
        aGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGt                                                                                                                                               >  2:877041/1‑150 (MQ=255)
         gcggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTc                                                                                                                                              <  2:673206/150‑1 (MQ=255)
          cggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCa                                                                                                                                             <  1:1422514/150‑1 (MQ=255)
           ggcgCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCAt                                                                                                                                            <  1:3535348/150‑1 (MQ=255)
             cgcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGc                                                                                                                                          >  2:4470142/1‑150 (MQ=255)
              gcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCt                                                                                                                                         <  2:893926/150‑1 (MQ=255)
              gcTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCt                                                                                                                                         >  2:2026319/1‑150 (MQ=255)
                tGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTgg                                                                                                                                       <  1:2648608/150‑1 (MQ=255)
                tGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTgg                                                                                                                                       >  2:4072985/1‑150 (MQ=255)
                   gTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGa                                                                                                                                    >  1:3009108/1‑150 (MQ=255)
                        gTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAAc                                                                                                                               <  2:3529926/150‑1 (MQ=255)
                         tGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAAcc                                                                                                                              <  2:401044/150‑1 (MQ=255)
                             ccTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGAc                                                                                                                          <  1:2809200/150‑1 (MQ=255)
                               tGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCa                                                                                                                        <  2:1773291/150‑1 (MQ=255)
                                gCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCAt                                                                                                                       <  2:4322893/150‑1 (MQ=255)
                                 aagTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCCTGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGAAAGGTGAAATCGACTATGTCATGCTGGCGAAAAACCCGACCATc                                                                                                                      >  1:495891/3‑150 (MQ=255)
                                  cGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCg                                                                                                                     >  1:938307/1‑150 (MQ=255)
                                   gTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGATATACCCGACCATCGa                                                                                                                    >  1:1756285/1‑150 (MQ=255)
                                    ttGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGAc                                                                                                                   <  2:1652929/150‑1 (MQ=255)
                                    ttGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGAc                                                                                                                   <  2:2471235/150‑1 (MQ=255)
                                     tGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACg                                                                                                                  <  1:3991489/150‑1 (MQ=255)
                                       cTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGcc                                                                                                                >  1:2113189/1‑150 (MQ=255)
                                       cTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGcc                                                                                                                <  1:3386764/150‑1 (MQ=255)
                                        tGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCt                                                                                                               <  2:4219346/150‑1 (MQ=255)
                                        tGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCt                                                                                                               <  1:3154647/150‑1 (MQ=255)
                                        tGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCt                                                                                                               <  1:3661445/150‑1 (MQ=255)
                                            cGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCtggtg                                                                                                           <  1:3548886/150‑1 (MQ=255)
                                             gAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCtggtgg                                                                                                          >  1:3886831/1‑150 (MQ=255)
                                             gAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGATGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCtggtgg                                                                                                          <  1:2322792/150‑1 (MQ=255)
                                               gTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGaa                                                                                                        <  1:1162816/150‑1 (MQ=255)
                                                tCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAg                                                                                                       >  1:4274262/1‑150 (MQ=255)
                                                  cGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCt                                                                                                     <  2:434969/150‑1 (MQ=255)
                                                  cGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCt                                                                                                     <  1:4616320/150‑1 (MQ=255)
                                                   gATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTa                                                                                                    <  1:4426050/150‑1 (MQ=255)
                                                     tGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTAct                                                                                                  <  1:3378500/150‑1 (MQ=255)
                                                     tGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCAGGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTAct                                                                                                  >  1:2277900/1‑150 (MQ=255)
                                                      gAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTActc                                                                                                 >  1:4683489/1‑150 (MQ=255)
                                                       aaCCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATTGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTActct                                                                                                <  2:2147425/150‑1 (MQ=255)
                                                           gTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCcg                                                                                            <  2:2959681/150‑1 (MQ=255)
                                                             tCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCcgcg                                                                                          <  2:3474727/150‑1 (MQ=255)
                                                              cGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGa                                                                                         >  1:3680009/1‑150 (MQ=255)
                                                               gATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAg                                                                                        <  2:3897647/150‑1 (MQ=255)
                                                               gATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAg                                                                                        <  1:2872937/150‑1 (MQ=255)
                                                                   acgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGa                                                                                    <  1:1239409/150‑1 (MQ=255)
                                                                   acgacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGa                                                                                    >  2:1722254/1‑150 (MQ=255)
                                                                     gacAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGaaa                                                                                  <  1:2281272/150‑1 (MQ=255)
                                                                      acAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAaat                                                                                 <  1:3147335/150‑1 (MQ=255)
                                                                      aaacccTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGAAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAaat                                                                                 <  1:1602950/146‑1 (MQ=255)
                                                                            tGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGa                                                                           <  2:263819/150‑1 (MQ=255)
                                                                            tGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGa                                                                           <  2:1934019/150‑1 (MQ=255)
                                                                             gATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAt                                                                          >  2:37416/1‑150 (MQ=255)
                                                                             gATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAt                                                                          <  2:1483625/150‑1 (MQ=255)
                                                                               tCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAttt                                                                        >  2:2802193/1‑150 (MQ=255)
                                                                               tCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGAttt                                                                        <  2:3935540/150‑1 (MQ=255)
                                                                                cGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTc                                                                       >  1:1568191/1‑150 (MQ=255)
                                                                                 gACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCa                                                                      >  1:899309/1‑150 (MQ=255)
                                                                                   cTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGc                                                                    <  1:2316821/150‑1 (MQ=255)
                                                                                     aCGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCgg                                                                  <  1:3321347/150‑1 (MQ=255)
                                                                                       ggTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTa                                                                >  2:4532875/1‑150 (MQ=255)
                                                                                       ggTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTa                                                                >  1:4065358/1‑150 (MQ=255)
                                                                                            ggATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAAt                                                           <  2:3009108/150‑1 (MQ=255)
                                                                                                 cGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTg                                                      >  1:2214167/1‑150 (MQ=255)
                                                                                                   gTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTggg                                                    >  2:1065638/1‑150 (MQ=255)
                                                                                                     gcgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTa                                                  <  1:3206846/150‑1 (MQ=255)
                                                                                                          tgctgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCTAAAAACCCGACCATCGACGCCTTGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCgg                                             <  1:2950273/147‑1 (MQ=255)
                                                                                                          tgatgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCgg                                             <  1:78422/150‑1 (MQ=255)
                                                                                                          tgatgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCgg                                             <  2:4136820/150‑1 (MQ=255)
                                                                                                           gatgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGgc                                            >  2:1257526/1‑150 (MQ=255)
                                                                                                            atgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGgcg                                           >  2:3080842/1‑150 (MQ=255)
                                                                                                            atgGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCACGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGgcg                                           >  2:3667312/1‑150 (MQ=255)
                                                                                                              ggCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCa                                         <  2:3041547/150‑1 (MQ=255)
                                                                                                                cgcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAgg                                       <  1:4416560/150‑1 (MQ=255)
                                                                                                                  cTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTa                                     >  2:103788/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaa                                    >  2:623383/1‑150 (MQ=255)
                                                                                                                   tGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTaa                                    <  1:3521259/150‑1 (MQ=255)
                                                                                                                         cgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                              >  1:1347078/1‑150 (MQ=255)
                                                                                                                         cgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                              >  2:3849946/1‑150 (MQ=255)
                                                                                                                         cgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                              <  1:2497856/150‑1 (MQ=255)
                                                                                                                         cgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                              >  2:1385511/1‑150 (MQ=255)
                                                                                                                          gcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATc                             <  2:540686/150‑1 (MQ=255)
                                                                                                                            gcTGGCGCAAAGTGCATGGTGATATAGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCgg                           <  1:3585520/150‑1 (MQ=255)
                                                                                                                                     aaGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCAc                  >  1:1184447/1‑150 (MQ=255)
                                                                                                                                       gTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACgg                >  1:4569527/1‑150 (MQ=255)
                                                                                                                                         gCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGgcg              >  1:3172555/1‑150 (MQ=255)
                                                                                                                                          cATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGgcgc             >  1:2229306/1‑150 (MQ=255)
                                                                                                                                           aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCt            <  1:2026319/150‑1 (MQ=255)
                                                                                                                                           aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCt            >  1:4131726/1‑150 (MQ=255)
                                                                                                                                              gTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGc         <  2:3231333/150‑1 (MQ=255)
                                                                                                                                              gTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGc         <  1:706222/150‑1 (MQ=255)
                                                                                                                                              gTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGc         <  1:4555407/150‑1 (MQ=255)
                                                                                                                                              gTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGc         <  1:1054325/150‑1 (MQ=255)
                                                                                                                                                  tatCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGttt     <  2:1695257/150‑1 (MQ=255)
                                                                                                                                                  tatCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGttt     <  2:7117/150‑1 (MQ=255)
                                                                                                                                                     cGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttgtt  <  2:2684196/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCAGCAAAGCGGCGCTGCGTGAGGTGATCCTGCCGTTGCTGGACGAGTCCGATGAACCGTTCGATGACGACAACCTGATCGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTT  >  CP009273/623786‑624084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑