Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 742,840 G→T 23.4% A129E (GCG→GAG)  abrB ← regulator of aidB expression; inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273742,8400GT23.4% 911.0 / 226.5 358A129E (GCG→GAG) abrBregulator of aidB expression; inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (132/142);  new base T (33/51);  total (165/193)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 367 total aligned reads displayed.

TGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTT  >  CP009273/742692‑742988
                                                                                                                                                    |                                                                                                                                                    
tGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTgcgc                                                                                                                                                     <  1:2954660/150‑1 (MQ=255)
tGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTgcgc                                                                                                                                                     >  1:36547/1‑150 (MQ=255)
 ggCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTcc                                                                                                                                                    >  2:767355/1‑150 (MQ=255)
    gggAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATa                                                                                                                                                 <  2:4192153/150‑1 (MQ=255)
     ggAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAg                                                                                                                                                <  2:2367576/150‑1 (MQ=255)
     ggAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAg                                                                                                                                                <  2:4294782/150‑1 (MQ=255)
     ggAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAg                                                                                                                                                <  2:1509400/150‑1 (MQ=255)
            gACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCAt                                                                                                                                         <  1:403331/150‑1 (MQ=255)
              cGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTg                                                                                                                                       <  2:2354032/150‑1 (MQ=255)
               gATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGACTATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                                                                                                                                      >  1:2760504/1‑150 (MQ=255)
               gATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgc                                                                                                                                      >  1:654221/1‑150 (MQ=255)
                   tGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTgccgcc                                                                                                                                  <  2:4515707/150‑1 (MQ=255)
                     cTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCcgccgc                                                                                                                                >  2:4708000/1‑150 (MQ=255)
                      tGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCcgccgcg                                                                                                                               <  2:2913021/150‑1 (MQ=255)
                           aaCCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCgccgcc                                                                                                                          <  1:3428080/150‑1 (MQ=255)
                             ccGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCag                                                                                                                        >  1:189716/1‑150 (MQ=255)
                             ccGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCag                                                                                                                        <  1:2333114/150‑1 (MQ=255)
                              cGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCcgg                                                                                                                       >  1:1088332/1‑150 (MQ=255)
                              cGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCagg                                                                                                                       <  2:1076231/150‑1 (MQ=255)
                                cTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATTTTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCaggag                                                                                                                     <  1:4451737/150‑1 (MQ=255)
                                    aGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCaggagagga                                                                                                                 <  1:756853/150‑1 (MQ=255)
                                      cGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGc                                                                                                               >  2:137087/1‑150 (MQ=255)
                                      cGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGc                                                                                                               <  1:3906884/150‑1 (MQ=255)
                                        ttATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGccc                                                                                                             <  2:3109828/150‑1 (MQ=255)
                                         tATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGcccc                                                                                                            <  2:2983228/150‑1 (MQ=255)
                                           tCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAg                                                                                                          >  2:864363/1‑150 (MQ=255)
                                           tCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAg                                                                                                          <  1:3153658/150‑1 (MQ=255)
                                                cAGCATCATGCTGGTTACCAGAACAGCGGCCACCGGGACGAACAGCACTCGAAGATATTGCATAAACGCCACCTGGCGAATATCTGCGCCGTTATCTTGAGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCAcc                                                                                                     <  1:2061450/150‑1 (MQ=255)
                                                 aGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCg                                                                                                    <  1:845948/150‑1 (MQ=255)
                                                 aGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCg                                                                                                    <  1:2373895/150‑1 (MQ=255)
                                                 aGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCg                                                                                                    <  1:212669/150‑1 (MQ=255)
                                                   catcatGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGt                                                                                                  <  2:3543619/150‑1 (MQ=255)
                                                    atcatGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTa                                                                                                 >  1:4403731/1‑150 (MQ=255)
                                                    atcatGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTa                                                                                                 <  2:1977300/150‑1 (MQ=255)
                                                     tcatGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTAt                                                                                                <  1:1062070/150‑1 (MQ=255)
                                                     tcatGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTAt                                                                                                >  1:254481/1‑150 (MQ=255)
                                                           gggTGACCCGCACAGCGGCCCCCGCGACGAACCGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAg                                                                                          <  1:127580/150‑1 (MQ=255)
                                                           gggTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAg                                                                                          <  1:1770148/150‑1 (MQ=255)
                                                            ggTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAgg                                                                                         >  1:1556785/1‑150 (MQ=255)
                                                                aCCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAAt                                                                                     <  2:1198290/150‑1 (MQ=255)
                                                                  cAGAACACCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGTATATCTGCGCCGTACTCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGa                                                                                   <  2:4222832/150‑1 (MQ=255)
                                                                   aGAACAGCGGCCCCCGCGTAGATCAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTATTCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGTGGAGCCCCAGGCACCGGTTTTTGCAGGCAATGAg                                                                                  <  2:4740963/150‑1 (MQ=255)
                                                                   aGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAg                                                                                  <  1:3801258/150‑1 (MQ=255)
                                                                   aGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAg                                                                                  <  2:2038667/150‑1 (MQ=255)
                                                                    gAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGc                                                                                 >  2:1781703/1‑150 (MQ=255)
                                                                    gAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGc                                                                                 >  2:2820640/1‑150 (MQ=255)
                                                                     aaCAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCt                                                                                <  2:1393541/150‑1 (MQ=255)
                                                                      aCAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCta                                                                               >  1:1563083/1‑150 (MQ=255)
                                                                       cAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCCCCGGTATTTCCAGGCAATGAGCtat                                                                              >  2:1628585/1‑150 (MQ=255)
                                                                        aGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCACTGAGCtata                                                                             >  2:4376759/1‑150 (MQ=255)
                                                                        aGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCtata                                                                             <  2:3692158/150‑1 (MQ=255)
                                                                        aGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCtata                                                                             >  2:339531/1‑150 (MQ=255)
                                                                        aGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCAGTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCtata                                                                             <  1:803178/150‑1 (MQ=255)
                                                                          cGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAgc                                                                           >  1:1074146/1‑150 (MQ=255)
                                                                           ggCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAgcg                                                                          <  1:486066/150‑1 (MQ=255)
                                                                             cccccGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCa                                                                        <  1:884617/150‑1 (MQ=255)
                                                                             cccccGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCa                                                                        <  1:2455480/150‑1 (MQ=255)
                                                                             cccccGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCa                                                                        >  2:334796/1‑150 (MQ=255)
                                                                              ccccGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCAc                                                                       >  2:385694/1‑150 (MQ=255)
                                                                                  gcgACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAAt                                                                   <  1:2587220/150‑1 (MQ=255)
                                                                                   cgacgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCGGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATa                                                                  >  2:4599470/1‑150 (MQ=255)
                                                                                   cgacgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATa                                                                  <  2:1706390/150‑1 (MQ=255)
                                                                                    gacgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATaa                                                                 >  1:1666767/1‑150 (MQ=255)
                                                                                     acgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATaac                                                                >  2:4013934/1‑150 (MQ=255)
                                                                                      cgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATaacc                                                               >  1:3251499/1‑150 (MQ=255)
                                                                                        aaCAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATaaccaa                                                             >  1:4353523/1‑150 (MQ=255)
                                                                                        aaCAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATaaccaa                                                             <  2:3454337/150‑1 (MQ=255)
                                                                                         aCAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATaaccaac                                                            >  2:1911208/1‑150 (MQ=255)
                                                                                            gCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCca                                                         >  1:3314358/1‑150 (MQ=255)
                                                                                            gCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCca                                                         <  1:4709074/150‑1 (MQ=255)
                                                                                            gCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCca                                                         <  1:4709143/150‑1 (MQ=255)
                                                                                             cACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCcac                                                        >  2:2627672/1‑150 (MQ=255)
                                                                                               cTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACaa                                                      <  1:3031393/150‑1 (MQ=255)
                                                                                                 cGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATg                                                    <  1:3143859/150‑1 (MQ=255)
                                                                                                   cAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGgc                                                  <  1:1789685/150‑1 (MQ=255)
                                                                                                       tatTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTg                                              <  1:4220397/150‑1 (MQ=255)
                                                                                                       tatTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTg                                              <  1:4291869/150‑1 (MQ=255)
                                                                                                        atTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTgg                                             <  1:3349862/150‑1 (MQ=255)
                                                                                                        atTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTgg                                             >  2:610264/1‑150 (MQ=255)
                                                                                                          tGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGaa                                           >  2:4791215/1‑150 (MQ=255)
                                                                                                           gCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGaaa                                          <  1:1324203/150‑1 (MQ=255)
                                                                                                            cATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAag                                         >  1:3197697/1‑150 (MQ=255)
                                                                                                            cATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAag                                         >  1:2992519/1‑150 (MQ=255)
                                                                                                             aTAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAagc                                        <  2:2125690/150‑1 (MQ=255)
                                                                                                               aaaCGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAagcag                                      >  2:2510832/1‑150 (MQ=255)
                                                                                                                  cGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGt                                   >  1:2713021/1‑150 (MQ=255)
                                                                                                                   gCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGtt                                  <  1:3479948/150‑1 (MQ=255)
                                                                                                                     caccaGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTAc                                >  2:4548752/1‑150 (MQ=255)
                                                                                                                        caGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAg                             >  1:2876471/1‑150 (MQ=255)
                                                                                                                          ggCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTa                           >  1:2289567/1‑150 (MQ=255)
                                                                                                                           gCGAATATCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTaa                          <  2:1062664/150‑1 (MQ=255)
                                                                                                                              aaTATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAAt                       <  2:21492/150‑1 (MQ=255)
                                                                                                                                tatCTGCGCCGTAATCTTGCTCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTg                     <  1:138583/150‑1 (MQ=255)
                                                                                                                                    tGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAg                 >  1:4601219/1‑150 (MQ=255)
                                                                                                                                         cGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTCAAATTGCAAGCACGa            >  2:4431391/1‑150 (MQ=255)
                                                                                                                                         cGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGa            >  2:2235412/1‑150 (MQ=255)
                                                                                                                                               cTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGcc      >  2:1848288/1‑150 (MQ=255)
                                                                                                                                               cTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGcc      <  2:3550308/150‑1 (MQ=255)
                                                                                                                                                 tGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAg    <  2:2760504/150‑1 (MQ=255)
                                                                                                                                                   cgcCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGtt  <  1:1997122/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTT  >  CP009273/742692‑742988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑