Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 743,278 G→A 24.9% intergenic (‑53/+99) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273743,2780GA24.9% 423.6 / 138.5 201intergenic (‑53/+99)abrB/ybgOregulator of aidB expression; inner membrane protein/putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (83/68);  new base A (22/28);  total (105/96)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

CCAAGTAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTC  >  CP009273/743131‑743427
                                                                                                                                                   |                                                                                                                                                     
ccAAGTAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCt                                                                                                                                                     <  2:3316936/150‑1 (MQ=255)
 cAAGTAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTCTTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTg                                                                                                                                                    >  2:547059/1‑150 (MQ=255)
  aaGTAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTgg                                                                                                                                                   <  1:4675036/150‑1 (MQ=255)
     tAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGcc                                                                                                                                                >  1:4609660/1‑150 (MQ=255)
     tAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGcc                                                                                                                                                >  2:3101095/1‑150 (MQ=255)
     tAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGcc                                                                                                                                                <  1:3122205/150‑1 (MQ=255)
     tAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACAGTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGcc                                                                                                                                                <  1:51492/150‑1 (MQ=255)
       aTAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAg                                                                                                                                              <  1:1279773/150‑1 (MQ=255)
       aTAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAg                                                                                                                                              <  1:1170250/150‑1 (MQ=255)
       aTAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACAGTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAg                                                                                                                                              <  2:3901879/150‑1 (MQ=255)
        tAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAgg                                                                                                                                             <  2:186369/150‑1 (MQ=255)
         aGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACAGTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGa                                                                                                                                            <  2:4311002/150‑1 (MQ=255)
           cgccgcCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGaaa                                                                                                                                          >  1:3234962/1‑150 (MQ=255)
           cgccgcCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGaaa                                                                                                                                          >  1:4718535/1‑150 (MQ=255)
              cgccgGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGaaaaaa                                                                                                                                       <  1:3519928/150‑1 (MQ=255)
                 cgGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATaa                                                                                                                                    <  1:4732859/150‑1 (MQ=255)
                  gggAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAAc                                                                                                                                   >  2:1517702/1‑150 (MQ=255)
                  gggAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAAc                                                                                                                                   >  1:1016194/1‑150 (MQ=255)
                   ggAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATACCCTTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACa                                                                                                                                  <  2:1436488/150‑1 (MQ=255)
                     agaTGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGc                                                                                                                                >  2:4415857/1‑150 (MQ=255)
                      gaTGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCa                                                                                                                               <  1:2078269/150‑1 (MQ=255)
                         gTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATc                                                                                                                            >  2:4736698/1‑150 (MQ=255)
                            ccGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAg                                                                                                                         <  2:166117/150‑1 (MQ=255)
                              gcgAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGa                                                                                                                       >  2:4072873/1‑150 (MQ=255)
                               cgAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGaa                                                                                                                      <  2:4388533/150‑1 (MQ=255)
                               cgAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACAGTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGaa                                                                                                                      <  2:1843774/150‑1 (MQ=255)
                                gAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGaaa                                                                                                                     <  2:1240520/150‑1 (MQ=255)
                                 aGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGaaaa                                                                                                                    >  1:4189146/1‑150 (MQ=255)
                                  ggAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAg                                                                                                                   >  1:1972957/1‑150 (MQ=255)
                                  ggAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAg                                                                                                                   <  1:1350858/150‑1 (MQ=255)
                                     aaCCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAgggg                                                                                                                <  1:785627/150‑1 (MQ=255)
                                     aaCCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAgggg                                                                                                                <  1:4759233/150‑1 (MQ=255)
                                       ccAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGaa                                                                                                              >  1:1809547/1‑150 (MQ=255)
                                        cAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGaaa                                                                                                             >  2:1409509/1‑150 (MQ=255)
                                           tAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATc                                                                                                          <  1:921911/150‑1 (MQ=255)
                                                aGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCaa                                                                                                     >  1:110239/1‑150 (MQ=255)
                                                    gaAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAAtttt                                                                                                 <  2:1287207/150‑1 (MQ=255)
                                                     aaGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTc                                                                                                >  2:3958954/1‑150 (MQ=255)
                                                     aaGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTc                                                                                                <  2:4468532/150‑1 (MQ=255)
                                                     aaGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTc                                                                                                >  2:3814544/1‑150 (MQ=255)
                                                       gTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTg                                                                                              >  2:2378625/1‑150 (MQ=255)
                                                       gTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTg                                                                                              <  2:2798032/150‑1 (MQ=255)
                                                        tGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGa                                                                                             <  1:1900034/150‑1 (MQ=255)
                                                        tGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGa                                                                                             <  1:1900001/150‑1 (MQ=255)
                                                         gATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGaa                                                                                            >  2:3765942/1‑150 (MQ=255)
                                                         gATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGaa                                                                                            >  2:842652/1‑150 (MQ=255)
                                                          aTAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGaaa                                                                                           >  1:3332861/1‑150 (MQ=255)
                                                          aTAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGaaa                                                                                           >  2:2002054/1‑150 (MQ=255)
                                                                cacaTAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATg                                                                                     <  2:4253782/150‑1 (MQ=255)
                                                                cacaTAACAGTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATg                                                                                     <  2:2179209/150‑1 (MQ=255)
                                                                     aaCATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCtt                                                                                >  2:891826/1‑150 (MQ=255)
                                                                     aaCATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCtt                                                                                >  2:894657/1‑150 (MQ=255)
                                                                      aCATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCttt                                                                               >  2:1487901/1‑150 (MQ=255)
                                                                        aTTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTcc                                                                             >  2:4040778/1‑150 (MQ=255)
                                                                              cACTGCAAAACTGGCATCCTTCGCTCCCTCATTTTTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCggcggc                                                                       <  2:833682/150‑1 (MQ=255)
                                                                                cTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCac                                                                     >  2:2932631/1‑149 (MQ=255)
                                                                                cTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCaa                                                                     >  2:3262610/1‑150 (MQ=255)
                                                                                 tGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCaaa                                                                    >  1:2118830/1‑150 (MQ=255)
                                                                                   cAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAgg                                                                  >  1:4380530/1‑150 (MQ=255)
                                                                                   cAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAgg                                                                  <  2:871532/150‑1 (MQ=255)
                                                                                     aaaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGc                                                                >  1:1258457/1‑150 (MQ=255)
                                                                                      aaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGca                                                               >  2:1741615/1‑150 (MQ=255)
                                                                                       aCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCTGGAAAAAATAACAGCAATCAAGGAAAAGTTGTAAATCAGCTATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGcat                                                              <  1:2777078/150‑1 (MQ=255)
                                                                                          ggCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGcatcat                                                           >  1:3873566/1‑150 (MQ=255)
                                                                                            cATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATCAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCAggg                                                         >  1:1718636/1‑150 (MQ=255)
                                                                                            cATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATgg                                                         >  1:2419527/1‑150 (MQ=255)
                                                                                             aTCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGt                                                        >  1:2773563/1‑150 (MQ=255)
                                                                                             aTCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGt                                                        >  2:1110853/1‑150 (MQ=255)
                                                                                              tCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTa                                                       >  2:703630/1‑150 (MQ=255)
                                                                                               ccTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTaa                                                      >  2:2144665/1‑150 (MQ=255)
                                                                                               ccTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTaa                                                      >  2:2143885/1‑150 (MQ=255)
                                                                                                   cGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCa                                                  <  1:3644830/150‑1 (MQ=255)
                                                                                                    gCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAg                                                 >  1:1928568/1‑150 (MQ=255)
                                                                                                      tCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGtt                                               >  1:2751738/1‑150 (MQ=255)
                                                                                                      tCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGtt                                               <  1:2163088/150‑1 (MQ=255)
                                                                                                      tCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGtt                                               <  1:4358333/150‑1 (MQ=255)
                                                                                                       cccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                              >  2:2748992/1‑150 (MQ=255)
                                                                                                       cccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTta                                              <  1:2517573/150‑1 (MQ=255)
                                                                                                          tCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTCAATCAGTTATAg                                           >  2:2746665/1‑150 (MQ=255)
                                                                                                           cATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGt                                          >  2:3355637/1‑150 (MQ=255)
                                                                                                               attTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAAt                                      <  1:1414548/150‑1 (MQ=255)
                                                                                                                     tctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAAGTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaa                                <  2:4158429/150‑1 (MQ=255)
                                                                                                                      ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaaa                               <  2:455674/150‑1 (MQ=255)
                                                                                                                      ctTTGGTGTATCGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaaa                               <  1:911433/150‑1 (MQ=255)
                                                                                                                         tGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGc                            >  1:4235407/1‑150 (MQ=255)
                                                                                                                             gtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcacca                        >  1:292588/1‑150 (MQ=255)
                                                                                                                              tATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGACAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCACAGGGCATCATGGTCAATCAGTTATAGTTAATCTTAAAAAGcaccac                       >  2:2832710/1‑150 (MQ=255)
                                                                                                                                 cGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGt                    >  2:3724517/1‑150 (MQ=255)
                                                                                                                                 cGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGt                    >  1:3757444/1‑150 (MQ=255)
                                                                                                                                 cGATACAGCATAAAGAAAACTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGt                    >  1:2225199/1‑150 (MQ=255)
                                                                                                                                   aTACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTAt                  <  2:1919012/150‑1 (MQ=255)
                                                                                                                                     aCAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCg                <  2:913414/150‑1 (MQ=255)
                                                                                                                                        gCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGACAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAACTCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAg             >  2:2528672/1‑150 (MQ=255)
                                                                                                                                         cATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgg            <  1:3501637/150‑1 (MQ=255)
                                                                                                                                           tAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgggg          >  1:2611396/1‑150 (MQ=255)
                                                                                                                                            aaagaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGc         >  1:3970012/1‑150 (MQ=255)
                                                                                                                                              agaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCa       <  1:3182923/150‑1 (MQ=255)
                                                                                                                                               gaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAg      >  2:3011999/1‑150 (MQ=255)
                                                                                                                                                  agCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCt   >  1:740905/1‑150 (MQ=255)
                                                                                                                                                  agCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCt   >  1:4322773/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCAAGTAATAGCGCCGCCGGGAGATGTACCGCGAGGAAACCAATAGAAAGGAGAAGTGATAGCACACATAACATTCCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTC  >  CP009273/743131‑743427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑