Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 772,664 A→C 12.4% K289N (AAA→AAC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273772,6640AC12.4% 439.0 / 10.9 193K289N (AAA→AAC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base A (151/11);  new base C (4/26);  total (156/37)
Fisher's exact test for biased strand distribution p-value = 1.84e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

AGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  CP009273/772517‑772813
                                                                                                                                                    |                                                                                                                                                     
agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGc                                                                                                                                                     >  1:515973/1‑150 (MQ=255)
agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGc                                                                                                                                                     >  1:516202/1‑150 (MQ=255)
agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGATGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGc                                                                                                                                                     >  1:1591818/1‑150 (MQ=255)
  agaAAGCTGCAGCCGACCAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAATAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCgg                                                                                                                                                   >  2:3592957/1‑150 (MQ=255)
  agaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCgg                                                                                                                                                   >  2:2851023/1‑150 (MQ=255)
   gaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGACAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGc                                                                                                                                                  >  1:4488701/1‑150 (MQ=255)
   gaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGc                                                                                                                                                  >  1:2184876/1‑150 (MQ=255)
     aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCg                                                                                                                                                >  1:3060622/1‑150 (MQ=255)
     aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCg                                                                                                                                                >  1:4668326/1‑150 (MQ=255)
            aGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGc                                                                                                                                         >  1:3723850/1‑150 (MQ=255)
                 aCAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatg                                                                                                                                    >  2:1321196/1‑150 (MQ=255)
                 aCAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatg                                                                                                                                    >  2:2107519/1‑150 (MQ=255)
                   aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatgat                                                                                                                                  >  1:2844777/1‑150 (MQ=255)
                   aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatgat                                                                                                                                  >  1:1313408/1‑150 (MQ=255)
                      aaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGAGGATAtt                                                                                                                               >  1:2137879/1‑150 (MQ=255)
                       aaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATAttt                                                                                                                              >  1:1816729/1‑150 (MQ=255)
                        agcagcAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATAtttt                                                                                                                             >  2:3022588/1‑150 (MQ=255)
                           agcagAAAAGGCGGGGGTTGAACCGGCTGCTGCTGATAAGAACGCCGGGGATGAAACCGCCGCCGCAGCCACACACGCGGCTGCGGCAA‑CCGCTGCTGCTGAAAACGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCgg                                                                                                                          <  2:4698658/150‑1 (MQ=255)
                             cagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTg                                                                                                                        >  1:3892333/1‑150 (MQ=255)
                              agAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGa                                                                                                                       >  2:1436740/1‑150 (MQ=255)
                               gAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAg                                                                                                                      >  2:1722893/1‑150 (MQ=255)
                                  aaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTa                                                                                                                   >  1:2227175/1‑150 (MQ=255)
                                  aaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTa                                                                                                                   >  2:4508949/1‑150 (MQ=255)
                                   acgCGGCTTCTGAAACGCCACCAGTTGATAAAAAAGCTGCGTCTTAAAACCCCGCCTAACAAAAAAATGGGGAAGCGGAAA‑AAGCGGCAGCTTAAAAAGCCGATGCAGAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                  <  2:1878862/148‑1 (MQ=255)
                                    aGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                 >  1:2353015/1‑150 (MQ=255)
                                      cGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGct                                                                                                               >  2:1787229/1‑150 (MQ=255)
                                         ctgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTg                                                                                                            >  2:743418/1‑150 (MQ=255)
                                          tgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTgg                                                                                                           >  2:4142872/1‑150 (MQ=255)
                                            ctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                         >  1:1066715/1‑150 (MQ=255)
                                            ctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                         >  2:1325829/1‑150 (MQ=255)
                                             tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                        >  2:4526661/1‑150 (MQ=255)
                                             tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                        >  1:1750440/1‑150 (MQ=255)
                                             tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                        >  1:610111/1‑150 (MQ=255)
                                                aaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                     >  2:4459535/1‑150 (MQ=255)
                                                     cagcagCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAc                                                                                                >  2:1793102/1‑150 (MQ=255)
                                                         agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                            >  1:2373392/1‑150 (MQ=255)
                                                         agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                            >  2:258824/1‑150 (MQ=255)
                                                         agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                            >  2:3139630/1‑150 (MQ=255)
                                                         agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                            >  1:4590096/1‑150 (MQ=255)
                                                           cTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaaaa                                                                                          >  1:1419581/1‑150 (MQ=255)
                                                            tGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAAc                                                                                         >  2:727729/1‑150 (MQ=255)
                                                              aTAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgg                                                                                       >  1:1800422/1‑150 (MQ=255)
                                                                aaGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                     >  1:2438357/1‑150 (MQ=255)
                                                                 aGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggggg                                                                                    >  1:1646654/1‑150 (MQ=255)
                                                                  gAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggggg                                                                                   >  1:3234579/1‑150 (MQ=255)
                                                                    aaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAg                                                                                 >  1:3942028/1‑150 (MQ=255)
                                                                          cgcaataaaacGCCGCCGCACACAAAAAAGCGGCTGCGGCAA‑AAGCGGCAGCTTAAAACCCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGa                                                                           <  2:4344056/144‑1 (MQ=255)
                                                                              aGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                       >  2:3693727/1‑150 (MQ=255)
                                                                              aGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                       >  1:440164/1‑150 (MQ=255)
                                                                               gaaactgccgcgGCTCACAAAAACGCGGCTGCGGCAA‑CCGCTGCAGCTGAAAACGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGATAggg                                                                      <  2:373986/144‑1 (MQ=255)
                                                                               gAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAggg                                                                      >  1:1498919/1‑150 (MQ=255)
                                                                               gAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAggg                                                                      >  1:3510577/1‑150 (MQ=255)
                                                                                  aacgccgccCCATACAACACCGCGGCCGCGGCAA‑ACGCTGCAGCTGAAAACGCCGCGGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAAc                                                                   <  2:3942028/147‑1 (MQ=255)
                                                                                    aGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCCGAGGCAGATGATATTTTCGGTGAGCTCAGCTCTGGTCAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACaa                                                                 >  2:2294648/1‑150 (MQ=255)
                                                                                          cgcAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTc                                                           >  2:3748660/1‑150 (MQ=255)
                                                                                           gcAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCg                                                          >  2:1056810/1‑150 (MQ=255)
                                                                                             aGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGcc                                                        >  2:2124403/1‑150 (MQ=255)
                                                                                             aGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGcc                                                        >  2:1933252/1‑150 (MQ=255)
                                                                                               caaaaaaaGGGGCCGGGGAAACACGCTGCGGCTCAAACCGCCGGTCCACAAACCGCGGCCGCCGAGGCAGTTGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCt                                                       <  2:528525/148‑1 (MQ=255)
                                                                                               aCAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTg                                                      >  1:1131953/1‑150 (MQ=255)
                                                                                                 aaaaaaGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    >  2:442861/1‑150 (MQ=255)
                                                                                                 aaaaaaGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    >  1:4569034/1‑150 (MQ=255)
                                                                                                  aaaaaGCGGCTGCGGAAA‑ATGCTGCTGCTGAAAACGCCGCTGCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCg                                                   <  2:4592566/150‑1 (MQ=255)
                                                                                                    aacggggcagcggcaA‑ACGCGGCAGCTTAAAACGCCGCTGCAGCACCCGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAACACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                 <  2:3947617/145‑1 (MQ=255)
                                                                                                    aaaGCGGCAGCTGCAA‑AAGCTGCAGCTTAAAACGCCGCTGAAGCAAACGCGGCCGAAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAATAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                 <  2:2203125/150‑1 (MQ=255)
                                                                                                    aaaGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                 >  1:859569/1‑150 (MQ=255)
                                                                                                    aaaGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                 >  1:859514/1‑150 (MQ=255)
                                                                                                     acgcgccagctccaA‑AAGCTCCAGCTCAAAAACCCGCTGCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                                <  2:3982633/148‑1 (MQ=255)
                                                                                                     aaGCGGCAGCGGCAA‑ACGCTGCTGCTTAAAACGCCGCTGCTGAAAACGCGGCCGCAGAGGCAGATTATATTTTCGGTTAGATAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                                <  2:4216393/150‑1 (MQ=255)
                                                                                                      agcggcagcggcAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAg                                               >  2:2776819/1‑150 (MQ=255)
                                                                                                         ggcagcggcaA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTgg                                            >  1:2971691/1‑150 (MQ=255)
                                                                                                          gcagcggcaA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGt                                           >  1:3786044/1‑150 (MQ=255)
                                                                                                               ggcaA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATAc                                      >  1:2371691/1‑150 (MQ=255)
                                                                                                                 caA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTa                                    >  1:2533248/1‑150 (MQ=255)
                                                                                                                 caA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTa                                    >  1:4136862/1‑150 (MQ=255)
                                                                                                                  aa‑aaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAATGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaa                                   >  2:589181/1‑150 (MQ=255)
                                                                                                                  aa‑aaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaa                                   >  1:582275/1‑150 (MQ=255)
                                                                                                                   a‑aaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                  >  1:2492669/1‑150 (MQ=255)
                                                                                                                       gcgcatgCTCAAAACCCCGCTGCACCAACCGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGATAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAAc                               <  2:1337010/144‑1 (MQ=255)
                                                                                                                        cTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACa                              >  2:3243364/1‑150 (MQ=255)
                                                                                                                        cTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACa                              >  2:483914/1‑150 (MQ=255)
                                                                                                                         tGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACaa                             >  1:2064710/1‑150 (MQ=255)
                                                                                                                              cTGAAAACGCCGCTGCATAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTTAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcg                        <  2:3434804/150‑1 (MQ=255)
                                                                                                                              cTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcg                        >  2:3866324/1‑150 (MQ=255)
                                                                                                                              cTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcg                        >  2:330748/1‑150 (MQ=255)
                                                                                                                              cTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcg                        >  2:1563381/1‑150 (MQ=255)
                                                                                                                                taaaacGCCGAGGAAGAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCa                      <  2:671091/149‑1 (MQ=255)
                                                                                                                                gAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCa                      >  2:1716863/1‑150 (MQ=255)
                                                                                                                                   acccccGCTGCAGCAACAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                   <  2:1724167/146‑1 (MQ=255)
                                                                                                                                    acgCCGGTGCAGAAAACGCGGCCGCAGTGGCAGATTATATTTTCGGTGAGATAAGCTCTGGTAAGAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAg                  <  2:4076951/148‑1 (MQ=255)
                                                                                                                                        cGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGc              >  1:2667072/1‑150 (MQ=255)
                                                                                                                                        cGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGc              >  1:2667711/1‑150 (MQ=255)
                                                                                                                                          cTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCg            >  2:1303885/1‑150 (MQ=255)
                                                                                                                                           tGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGa           >  2:1568862/1‑150 (MQ=255)
                                                                                                                                                caAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCa      >  2:1959091/1‑150 (MQ=255)
                                                                                                                                                 aaaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa     >  1:2904372/1‑150 (MQ=255)
                                                                                                                                                 aaaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa     >  2:617484/1‑150 (MQ=255)
                                                                                                                                                  aaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAAt    >  2:3786647/1‑150 (MQ=255)
                                                                                                                                                    aGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATaa  <  2:2671476/150‑1 (MQ=255)
                                                                                                                                                    aGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATaa  >  1:3306773/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAA‑AAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  CP009273/772517‑772813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑