Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 818,362 G→T 33.8% G278G (GGC→GGA)  clsB ← cardiolipin synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273818,3620GT33.8% 462.8 / 262.0 277G278G (GGC→GGA) clsBcardiolipin synthase 2
Reads supporting (aligned to +/- strand):  ref base G (90/93);  new base T (46/48);  total (136/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

AATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGT  >  CP009273/818214‑818510
                                                                                                                                                     |                                                                                                                                                    
  tGACGATGGTGGATGATGACTTTTTCTTCGAGATTCAGTGACAAACTTAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCAATTTGCCGTGGAGGGGGCGGCGGCGTTACTC‑AAAAACCTGAAcgccg                                                                                                                                                   <  1:2803421/150‑1 (MQ=255)
  tGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAAcgccg                                                                                                                                                   <  2:1614641/150‑1 (MQ=255)
     cGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCt                                                                                                                                                <  1:4318944/150‑1 (MQ=255)
      gATCGTTGATGATGACATTTGCTTCGAGATTCAGTGACATCCTGAGCTGTTCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTTGAGCGGGGGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCtt                                                                                                                                               <  1:4604299/150‑1 (MQ=255)
      gATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCtt                                                                                                                                               >  1:3035337/1‑150 (MQ=255)
      gATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCtt                                                                                                                                               >  2:2719527/1‑150 (MQ=255)
       aTCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCttt                                                                                                                                              <  1:4068234/150‑1 (MQ=255)
       aTCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCttt                                                                                                                                              <  1:1908973/150‑1 (MQ=255)
       aTCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCttt                                                                                                                                              <  1:4253501/150‑1 (MQ=255)
        tCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTa                                                                                                                                             <  2:755329/150‑1 (MQ=255)
           tGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGAACGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAAcc                                                                                                                                          <  2:13278/150‑1 (MQ=255)
             tattatgaCATTTGCTTCGATATTAAGTGACAAACTTAGGGTATCGAGATTACTGGACCCTACTGTCTCCCAGTGATAGTCCATAAATGCCACTTTGCCGTGTAGCGGGCGTCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAg                                                                                                                                        <  1:4494291/146‑1 (MQ=255)
             gatgatGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAg                                                                                                                                        >  1:1852588/1‑150 (MQ=255)
             gatgatGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCACCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAg                                                                                                                                        <  1:2950530/150‑1 (MQ=255)
              atgatgACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGa                                                                                                                                       >  1:1136844/1‑150 (MQ=255)
              atgatgACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGa                                                                                                                                       >  2:4563486/1‑150 (MQ=255)
                  tgaCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGt                                                                                                                                   >  2:3788276/1‑150 (MQ=255)
                    acatgagCTTAGACATTCAGTGACAAACTGAGCGGATCGATATTACTGGCCCCCACGGTCGCCCAGTGAGCGTCCAGACATTCCAATTTGCCTTGTAGCGGGCGGCGGCGGTACTC‑AAAACCCTGAACGCCGCCTTTAACCAGATAGTta                                                                                                                                 <  2:169794/144‑1 (MQ=255)
                    aCATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTta                                                                                                                                 >  2:4392983/1‑150 (MQ=255)
                     cATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTtat                                                                                                                                >  1:4664317/1‑150 (MQ=255)
                     cATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTtat                                                                                                                                <  1:2378389/150‑1 (MQ=255)
                      aTTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTtata                                                                                                                               >  2:1292910/1‑150 (MQ=255)
                         tGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAg                                                                                                                            >  2:2281012/1‑150 (MQ=255)
                          gCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGc                                                                                                                           <  1:3515588/150‑1 (MQ=255)
                          gCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGc                                                                                                                           <  1:4509170/150‑1 (MQ=255)
                               gagaTTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAgcg                                                                                                                      >  1:537233/1‑150 (MQ=255)
                                   ttCAGTTACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCAACAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCAGAACGCCGCCTTTAACCAGATAGTTAGACAGCAAGCGCGCa                                                                                                                  >  2:3600198/1‑150 (MQ=255)
                                     cAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCAcc                                                                                                                >  2:4458932/1‑150 (MQ=255)
                                         gACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGAct                                                                                                            <  1:2434799/150‑1 (MQ=255)
                                               cTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACa                                                                                                      >  2:2827938/1‑150 (MQ=255)
                                               cTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACa                                                                                                      >  2:383738/1‑150 (MQ=255)
                                               cTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACa                                                                                                      >  2:3017115/1‑150 (MQ=255)
                                                 gAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAAt                                                                                                    <  1:1085715/150‑1 (MQ=255)
                                                  aGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATc                                                                                                   <  2:3829456/150‑1 (MQ=255)
                                                   gCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCg                                                                                                  <  2:3856357/150‑1 (MQ=255)
                                                         cGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCatat                                                                                            <  2:4166501/150‑1 (MQ=255)
                                                          gagaTTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATc                                                                                           >  1:4405920/1‑150 (MQ=255)
                                                             aTTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCgg                                                                                        >  2:4672270/1‑150 (MQ=255)
                                                              ttACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGt                                                                                       >  1:4079325/1‑150 (MQ=255)
                                                               tACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGt                                                                                       <  1:684742/150‑1 (MQ=255)
                                                                aCTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTc                                                                                     <  1:3919189/150‑1 (MQ=255)
                                                                  tGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGc                                                                                   >  2:1054410/1‑150 (MQ=255)
                                                                    gACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGccc                                                                                 >  2:4234068/1‑150 (MQ=255)
                                                                     aCCCTACTGTCGCCCTGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCt                                                                                <  1:1161704/150‑1 (MQ=255)
                                                                     aCCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCt                                                                                <  1:3146677/150‑1 (MQ=255)
                                                                      cccTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTg                                                                               <  2:3064078/150‑1 (MQ=255)
                                                                       ccTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGa                                                                              <  1:194050/150‑1 (MQ=255)
                                                                       ccTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGa                                                                              >  2:3993773/1‑150 (MQ=255)
                                                                       ccTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGa                                                                              >  2:4710835/1‑150 (MQ=255)
                                                                           cTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGa                                                                          <  2:1848514/150‑1 (MQ=255)
                                                                            tGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGAt                                                                         <  2:4007235/150‑1 (MQ=255)
                                                                               cGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAg                                                                      <  2:90305/150‑1 (MQ=255)
                                                                                gCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGt                                                                     >  2:2668166/1‑150 (MQ=255)
                                                                                  ccAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGttt                                                                   <  2:591042/150‑1 (MQ=255)
                                                                                    aGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGa                                                                 >  2:3691171/1‑150 (MQ=255)
                                                                                          cGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCTGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCa                                                           <  1:905888/150‑1 (MQ=255)
                                                                                           gTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCAc                                                          >  2:249984/1‑150 (MQ=255)
                                                                                            tCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCAcc                                                         >  2:1501073/1‑150 (MQ=255)
                                                                                              cATCAAGGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCAcccc                                                       <  1:2457242/150‑1 (MQ=255)
                                                                                               aTCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCcg                                                      >  1:1744998/1‑150 (MQ=255)
                                                                                               aTCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCcg                                                      >  2:2920145/1‑150 (MQ=255)
                                                                                                        aCTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGcc                                             <  2:4653496/150‑1 (MQ=255)
                                                                                                         cTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCg                                            >  1:1663420/1‑150 (MQ=255)
                                                                                                           ttGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCt                                          >  2:1166856/1‑150 (MQ=255)
                                                                                                             gCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCttt                                        <  1:2007564/150‑1 (MQ=255)
                                                                                                              ccGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTa                                       <  1:4643088/150‑1 (MQ=255)
                                                                                                              ccGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATCGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGAGCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTa                                       >  2:1144957/1‑150 (MQ=255)
                                                                                                              ccGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTa                                       >  2:4304973/1‑150 (MQ=255)
                                                                                                               cGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTAc                                      <  1:2695531/150‑1 (MQ=255)
                                                                                                                gTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACg                                     <  1:2207441/150‑1 (MQ=255)
                                                                                                                  ggAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCa                                   <  2:3327911/150‑1 (MQ=255)
                                                                                                                    aGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAg                                 <  2:3035357/150‑1 (MQ=255)
                                                                                                                      cgggcggCGGCGGTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGc                               >  2:206630/1‑150 (MQ=255)
                                                                                                                      cgggcggCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGc                               <  1:4299000/150‑1 (MQ=255)
                                                                                                                        ggcggcggcGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCgt                             <  2:590709/150‑1 (MQ=255)
                                                                                                                          cggcggcggTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCgtgt                           >  1:2101833/1‑150 (MQ=255)
                                                                                                                           ggcggcggTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTa                          >  2:3449862/1‑150 (MQ=255)
                                                                                                                           ggcggcggTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTa                          >  2:1420119/1‑150 (MQ=255)
                                                                                                                             cggcggTACTC‑AAAAACCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAAAGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTaaa                        >  1:3714425/1‑150 (MQ=255)
                                                                                                                               gcggTACTC‑AACAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTaaaaa                      <  2:2582582/150‑1 (MQ=255)
                                                                                                                                 ggTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATc                    <  1:1677/150‑1 (MQ=255)
                                                                                                                                 ggTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATc                    <  1:3402535/150‑1 (MQ=255)
                                                                                                                                 ggTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATc                    >  2:4519215/1‑150 (MQ=255)
                                                                                                                                  gTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCg                   <  2:4757241/150‑1 (MQ=255)
                                                                                                                                       c‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGc              <  1:2719527/150‑1 (MQ=255)
                                                                                                                                         aaaaaCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGcc             <  1:2615397/150‑1 (MQ=255)
                                                                                                                                          aaaaCCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCg            >  2:604676/1‑150 (MQ=255)
                                                                                                                                          aaaaCCTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCg            >  2:686067/1‑150 (MQ=255)
                                                                                                                                             aCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCgggg         >  1:1423732/1‑150 (MQ=255)
                                                                                                                                              ccTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGga        <  2:886952/150‑1 (MQ=255)
                                                                                                                                               cTGAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaa       >  2:1804951/1‑150 (MQ=255)
                                                                                                                                                 gAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaaga     <  2:2377971/150‑1 (MQ=255)
                                                                                                                                                 gAACTCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaaga     <  2:397001/150‑1 (MQ=255)
                                                                                                                                                 gAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaaga     >  2:788156/1‑150 (MQ=255)
                                                                                                                                                  aaCGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaagaa    >  2:4118990/1‑150 (MQ=255)
                                                                                                                                                  aaCGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaagaa    >  2:3944605/1‑150 (MQ=255)
                                                                                                                                                  aaCGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaagaa    <  2:3534052/150‑1 (MQ=255)
                                                                                                                                                  aaCGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaagaa    >  2:2315309/1‑150 (MQ=255)
                                                                                                                                                  aaCGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGgaagaa    <  1:259622/150‑1 (MQ=255)
                                                                                                                                                    cgccgcCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGt  >  1:4606274/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTC‑AAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGCAAGGCGTGTAAAAATCGATAGCCGGGGAAGAAGT  >  CP009273/818214‑818510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑