Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 968,415 G→T 91.9% S148* (TCG→TAG)  ycbC ← DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273968,4150GT91.9% 991.0 / 73.9 330S148* (TCG→TAG) ycbCDUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (11/16);  new base T (116/187);  total (127/203)
Fisher's exact test for biased strand distribution p-value = 8.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 331 total aligned reads displayed.

GAAAAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTA  >  CP009273/968266‑968561
                                                                                                                                                     |                                                                                                                                                  
gAAAAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCg                                                                                                                                                    <  1:1569710/150‑1 (MQ=255)
   aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAtt                                                                                                                                                 <  2:923081/150‑1 (MQ=255)
   aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAtt                                                                                                                                                 <  2:2394338/150‑1 (MQ=255)
   aaaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTAtt                                                                                                                                                 <  2:561874/150‑1 (MQ=255)
      aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgcg                                                                                                                                              <  2:3523434/150‑1 (MQ=255)
       gATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgcgc                                                                                                                                             <  1:3120816/150‑1 (MQ=255)
       gATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTgcgc                                                                                                                                             >  1:3713002/1‑150 (MQ=255)
        atcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCa                                                                                                                                            <  1:1905221/150‑1 (MQ=255)
         tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCaa                                                                                                                                           <  2:1700310/150‑1 (MQ=255)
           atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAAct                                                                                                                                         <  2:2869659/150‑1 (MQ=255)
           atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAAct                                                                                                                                         <  2:1046881/150‑1 (MQ=255)
             cGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAActct                                                                                                                                       <  2:2038056/150‑1 (MQ=255)
             cGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAActct                                                                                                                                       <  1:4491058/150‑1 (MQ=255)
                    ggCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCt                                                                                                                                <  1:2421993/150‑1 (MQ=255)
                    ggCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCt                                                                                                                                <  1:485086/150‑1 (MQ=255)
                        gATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACtt                                                                                                                            >  2:1148489/1‑150 (MQ=255)
                          tgtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTcc                                                                                                                          >  2:3885501/1‑150 (MQ=255)
                          tgtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTcc                                                                                                                          >  2:3885610/1‑150 (MQ=255)
                           gtgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCg                                                                                                                         <  1:3490955/150‑1 (MQ=255)
                            tgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGc                                                                                                                        >  2:4355104/1‑150 (MQ=255)
                            tgATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGc                                                                                                                        >  2:713155/1‑150 (MQ=255)
                             gATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCt                                                                                                                       <  2:1348122/150‑1 (MQ=255)
                             gATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCt                                                                                                                       <  1:400561/150‑1 (MQ=255)
                                 cGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTAc                                                                                                                   <  2:3643481/150‑1 (MQ=255)
                                  ggAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACt                                                                                                                  >  1:4263468/1‑150 (MQ=255)
                                        cACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGt                                                                                                            >  2:3116575/1‑150 (MQ=255)
                                          ccAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAt                                                                                                          <  1:3615437/150‑1 (MQ=255)
                                          ccAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAt                                                                                                          <  1:381102/150‑1 (MQ=255)
                                          ccAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAt                                                                                                          <  1:3857272/150‑1 (MQ=255)
                                           cagcagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAtt                                                                                                         >  2:1574654/1‑150 (MQ=255)
                                              cagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTCTTGGt                                                                                                      >  2:4406045/1‑150 (MQ=255)
                                              cagGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGt                                                                                                      <  1:4030586/150‑1 (MQ=255)
                                               agGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtt                                                                                                     <  2:3146694/150‑1 (MQ=255)
                                               agGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtt                                                                                                     <  2:3766253/150‑1 (MQ=255)
                                               agGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtt                                                                                                     <  1:2260861/150‑1 (MQ=255)
                                                ggAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTCTCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTACGCTGTACTCACCGTATTGGttt                                                                                                    <  1:1292806/150‑1 (MQ=255)
                                                 gAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                                                   <  2:1779386/150‑1 (MQ=255)
                                                 gAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                                                   >  2:3933605/1‑150 (MQ=255)
                                                       ggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTAc                                                                                             <  2:2700262/150‑1 (MQ=255)
                                                       ggCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTAc                                                                                             <  1:4730012/150‑1 (MQ=255)
                                                        gCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACg                                                                                            <  2:4548281/150‑1 (MQ=255)
                                                        gCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACg                                                                                            <  1:1095534/150‑1 (MQ=255)
                                                          gTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGcc                                                                                          <  2:2087405/150‑1 (MQ=255)
                                                          gTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGcc                                                                                          >  1:3682684/1‑150 (MQ=255)
                                                             aCCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTcc                                                                                       <  2:2610708/150‑1 (MQ=255)
                                                              ccAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTccc                                                                                      <  2:3970003/150‑1 (MQ=255)
                                                              ccAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTccc                                                                                      <  1:3190010/150‑1 (MQ=255)
                                                                 aTCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTg                                                                                   >  1:3344402/1‑150 (MQ=255)
                                                                    gCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAg                                                                                <  2:2790706/150‑1 (MQ=255)
                                                                       tGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATc                                                                             >  1:4126272/1‑150 (MQ=255)
                                                                             aCTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGtttt                                                                       >  1:3841745/1‑150 (MQ=255)
                                                                              ctgctgCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTg                                                                      <  1:3763274/150‑1 (MQ=255)
                                                                              ctgctgCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTg                                                                      <  2:1858272/150‑1 (MQ=255)
                                                                               tgctgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGa                                                                     <  1:1634657/150‑1 (MQ=255)
                                                                                gctgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGAt                                                                    <  1:1259632/150‑1 (MQ=255)
                                                                                  tgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATcc                                                                  <  1:4165930/150‑1 (MQ=255)
                                                                                  tgcAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATcc                                                                  <  1:2798188/150‑1 (MQ=255)
                                                                                     aGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCgg                                                               <  1:1007547/150‑1 (MQ=255)
                                                                                     aGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCgg                                                               <  2:1388988/150‑1 (MQ=255)
                                                                                        ttcttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGAtt                                                            <  2:3078252/150‑1 (MQ=255)
                                                                                           ttcttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTc                                                         <  2:3492446/150‑1 (MQ=255)
                                                                                             cttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTcgc                                                       <  1:2462040/150‑1 (MQ=255)
                                                                                              ttcGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTcgcg                                                      >  2:4761523/1‑150 (MQ=255)
                                                                                                  gTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCAt                                                  >  1:1411108/1‑150 (MQ=255)
                                                                                                      cTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGc                                              >  1:1808/1‑150 (MQ=255)
                                                                                                      cTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGc                                              <  1:2544160/150‑1 (MQ=255)
                                                                                                       ttttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCg                                             <  1:4658150/150‑1 (MQ=255)
                                                                                                       ttttGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCg                                             <  1:3934222/150‑1 (MQ=255)
                                                                                                          tGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAAt                                          <  2:3674346/150‑1 (MQ=255)
                                                                                                            gCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATAc                                        <  2:3252730/150‑1 (MQ=255)
                                                                                                             cAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATAcc                                       <  1:831580/150‑1 (MQ=255)
                                                                                                             cAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATAcc                                       >  1:2439087/1‑150 (MQ=255)
                                                                                                                 tCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttca                                   <  1:2210803/150‑1 (MQ=255)
                                                                                                                     gggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattca                               >  2:1244732/1‑150 (MQ=255)
                                                                                                                     gggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCttcattca                               <  2:812493/150‑1 (MQ=255)
                                                                                                                      ggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAg                              <  2:4623398/150‑1 (MQ=255)
                                                                                                                      ggTGATAATTTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAg                              <  1:322193/150‑1 (MQ=255)
                                                                                                                       gTGATAATGTGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAgg                             <  2:2955094/150‑1 (MQ=255)
                                                                                                                              tttGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCa                      <  1:1515376/150‑1 (MQ=255)
                                                                                                                               ttGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAg                     >  2:251320/1‑150 (MQ=255)
                                                                                                                                tGCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGa                    >  1:3168080/1‑150 (MQ=255)
                                                                                                                                 gCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGAc                   >  2:223532/1‑150 (MQ=255)
                                                                                                                                 gCTCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGAc                   <  1:3390355/150‑1 (MQ=255)
                                                                                                                                   tCGCGCGGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTg                 <  1:2761535/150‑1 (MQ=255)
                                                                                                                                     gcgcgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGtt               <  1:97599/150‑1 (MQ=255)
                                                                                                                                        cgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTAtt            >  1:1189079/1‑150 (MQ=255)
                                                                                                                                        cgGCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTAtt            >  2:3017022/1‑150 (MQ=255)
                                                                                                                                         ggCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTg           >  2:1634585/1‑150 (MQ=255)
                                                                                                                                         ggCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTg           >  1:2957016/1‑150 (MQ=255)
                                                                                                                                         ggCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGG‑TTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGa          >  2:627637/1‑150 (MQ=255)
                                                                                                                                          gCACGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGa          <  2:3031255/150‑1 (MQ=255)
                                                                                                                                            aCGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTCTTGAtt        >  2:4130125/1‑150 (MQ=255)
                                                                                                                                            aCGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGAtt        <  1:1956404/150‑1 (MQ=255)
                                                                                                                                             cGCCCAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTa       <  2:4649754/150‑1 (MQ=255)
                                                                                                                                              gCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTaa      >  1:2648887/1‑150 (MQ=255)
                                                                                                                                                ccAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAAt    <  1:655971/150‑1 (MQ=255)
                                                                                                                                                 cAGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAAtt   <  1:713155/150‑1 (MQ=255)
                                                                                                                                                  aGCTATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTa  >  1:744255/1‑150 (MQ=255)
                                                                                                                                                  aGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTa  >  2:1875152/1‑150 (MQ=255)
                                                                                                                                                  aGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTa  >  2:1090577/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GAAAAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAGGGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCAGGCGTGGCAGACTGTTATTGATTAAATTA  >  CP009273/968266‑968561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑