Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,073,243 C→A 27.5% G366C (GGT→TGT)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,073,2430CA27.5% 682.5 / 238.1 319G366C (GGT→TGT) putAfused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (131/100);  new base A (58/30);  total (189/130)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 324 total aligned reads displayed.

ATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGT  >  CP009273/1073094‑1073392
                                                                                                                                                     |                                                                                                                                                     
aTCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATAcc                                                                                                                                                       >  1:4608272/1‑150 (MQ=255)
  cGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATAccta                                                                                                                                                     <  2:1504056/150‑3 (MQ=255)
   gATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAat                                                                                                                                                    >  1:186344/1‑150 (MQ=255)
    aTCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAata                                                                                                                                                   >  2:3281206/1‑150 (MQ=255)
      cACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATc                                                                                                                                                 >  2:3076222/1‑150 (MQ=255)
       aCCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCg                                                                                                                                                <  1:3069462/150‑1 (MQ=255)
         cAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTa                                                                                                                                              >  1:2833826/1‑150 (MQ=255)
         cAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTa                                                                                                                                              <  1:2049075/150‑1 (MQ=255)
            cGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTAc                                                                                                                                             <  1:3320897/148‑1 (MQ=255)
             gggCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGa                                                                                                                                          >  2:513360/1‑150 (MQ=255)
              ggCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCGGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGAc                                                                                                                                         >  2:3620077/1‑150 (MQ=255)
                   cGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGcc                                                                                                                                    <  1:3534393/150‑1 (MQ=255)
                    gTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCca                                                                                                                                   <  2:570218/150‑1 (MQ=255)
                     tttttGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCCCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCcag                                                                                                                                  >  1:4525137/1‑150 (MQ=255)
                      ttttGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCcagc                                                                                                                                 >  2:3209025/1‑150 (MQ=255)
                      ttttGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTACCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCcagc                                                                                                                                 >  2:1053146/1‑150 (MQ=255)
                         tGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAgg                                                                                                                              >  2:18991/1‑150 (MQ=255)
                              aGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGgtgag                                                                                                                         <  1:1134551/150‑1 (MQ=255)
                                ccTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGgtgagtg                                                                                                                       >  1:2454520/1‑150 (MQ=255)
                                 cTGAATCACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGgtgagtga                                                                                                                      <  2:10064/150‑1 (MQ=255)
                                 cTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGgtgagtga                                                                                                                      >  1:282328/1‑150 (MQ=255)
                                  tGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGAt                                                                                                                     >  2:767019/1‑150 (MQ=255)
                                   gAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGAtt                                                                                                                    <  2:1159968/150‑1 (MQ=255)
                                     aTAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTc                                                                                                                  >  2:130358/1‑150 (MQ=255)
                                     aTAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTc                                                                                                                  >  1:3030527/1‑150 (MQ=255)
                                     aTAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTc                                                                                                                  >  2:2576177/1‑150 (MQ=255)
                                        aCAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGa                                                                                                               >  1:504366/1‑150 (MQ=255)
                                         cAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGAc                                                                                                              >  1:1397871/1‑150 (MQ=255)
                                         cAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGAc                                                                                                              <  1:4629030/150‑1 (MQ=255)
                                          aaaaCCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGAcg                                                                                                             >  2:2793801/1‑150 (MQ=255)
                                            aaCCGAGGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGAcgcg                                                                                                           <  2:3520964/150‑1 (MQ=255)
                                              ccGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCggg                                                                                                         <  2:1244744/150‑1 (MQ=255)
                                               cGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGt                                                                                                        <  2:2309191/150‑1 (MQ=255)
                                                  tGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTaaa                                                                                                     >  2:4051056/1‑150 (MQ=255)
                                                   gCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAg                                                                                                    >  2:4750446/1‑150 (MQ=255)
                                                   gCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAg                                                                                                    <  1:426351/150‑1 (MQ=255)
                                                      gTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGctc                                                                                                 >  1:4393515/1‑150 (MQ=255)
                                                        tCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCtt                                                                                               >  1:3200767/1‑150 (MQ=255)
                                                          cAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTcc                                                                                             <  2:2460812/150‑1 (MQ=255)
                                                           aGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCa                                                                                            <  1:702571/150‑1 (MQ=255)
                                                              cTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTa                                                                                         <  1:978252/150‑1 (MQ=255)
                                                               tgcaatttCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCTGCGTCAATGTTGATACCAATATCGTAATGACTCGCCAGCAGGGTGAGTGATTTCAGACTCGGGTAAAGCTCTTCCATAAc                                                                                        >  2:20691/7‑150 (MQ=255)
                                                                   aGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCgg                                                                                    >  1:3358680/1‑150 (MQ=255)
                                                                       ccGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCAt                                                                                <  2:223248/150‑1 (MQ=255)
                                                                        cGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATa                                                                               <  2:483820/150‑1 (MQ=255)
                                                                          gCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACt                                                                             <  2:1734775/150‑1 (MQ=255)
                                                                           cTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTg                                                                            <  1:3150928/150‑1 (MQ=255)
                                                                            tCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTgg                                                                           >  1:415612/1‑150 (MQ=255)
                                                                            tCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTgg                                                                           >  2:1339096/1‑150 (MQ=255)
                                                                              gAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGgc                                                                         >  2:413507/1‑150 (MQ=255)
                                                                              gAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGgc                                                                         >  1:98523/1‑150 (MQ=255)
                                                                                  cAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCgg                                                                     >  1:1070857/1‑150 (MQ=255)
                                                                                    gagTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCt                                                                   <  1:428624/150‑1 (MQ=255)
                                                                                     agTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCta                                                                  >  1:4080200/1‑150 (MQ=255)
                                                                                       tttttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCtata                                                                >  1:1030730/1‑150 (MQ=255)
                                                                                       tttttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCtata                                                                <  1:1359782/150‑1 (MQ=255)
                                                                                       tttttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCtata                                                                <  1:2237504/150‑1 (MQ=255)
                                                                                       tttttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCtata                                                                >  1:2870543/1‑150 (MQ=255)
                                                                                         tttCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTCTAAc                                                              >  2:3384414/1‑150 (MQ=255)
                                                                                               gcagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGAt                                                        <  1:1729471/150‑1 (MQ=255)
                                                                                                cagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATg                                                       <  2:4646994/150‑1 (MQ=255)
                                                                                                cagATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATg                                                       >  2:3852999/1‑150 (MQ=255)
                                                                                                 agATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGTAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGc                                                      >  2:2749803/1‑150 (MQ=255)
                                                                                                 agATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGc                                                      >  1:1832063/1‑150 (MQ=255)
                                                                                                 agATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGc                                                      >  1:2673181/1‑150 (MQ=255)
                                                                                                  gATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCa                                                     <  1:94946/150‑1 (MQ=255)
                                                                                                  gATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCa                                                     <  1:3610481/150‑1 (MQ=255)
                                                                                                  gATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCa                                                     <  2:2833825/150‑1 (MQ=255)
                                                                                                   aTCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAg                                                    <  2:1742739/150‑1 (MQ=255)
                                                                                                   aTCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAg                                                    <  2:3705355/150‑1 (MQ=255)
                                                                                                   aTCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAg                                                    >  1:2182126/1‑150 (MQ=255)
                                                                                                     cGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAgcg                                                  >  1:1481078/1‑150 (MQ=255)
                                                                                                      gAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAgcgc                                                 >  2:3722400/1‑150 (MQ=255)
                                                                                                         ggAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGa                                              >  2:1956888/1‑150 (MQ=255)
                                                                                                         ggAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCGATAACGCGGATGCAGCGCCGa                                              >  1:2870701/1‑150 (MQ=255)
                                                                                                           agaTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACa                                            <  1:2704793/150‑1 (MQ=255)
                                                                                                           agaTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACa                                            >  1:233392/1‑150 (MQ=255)
                                                                                                            gaTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAg                                           <  1:1184748/150‑1 (MQ=255)
                                                                                                             aTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGt                                          >  1:3598344/1‑150 (MQ=255)
                                                                                                              tctcCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGtt                                         <  1:2541985/150‑1 (MQ=255)
                                                                                                                  cAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgat                                     <  2:4393515/150‑1 (MQ=255)
                                                                                                                   aGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgatt                                    <  1:4738749/150‑1 (MQ=255)
                                                                                                                   aGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgatt                                    >  2:1411754/1‑150 (MQ=255)
                                                                                                                    ggCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTttgattg                                   <  2:2666611/150‑1 (MQ=255)
                                                                                                                      cGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGaa                                 >  2:74751/1‑150 (MQ=255)
                                                                                                                       gATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGaaa                                >  1:857918/1‑150 (MQ=255)
                                                                                                                       gATCGGACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGaaa                                <  1:3884545/150‑1 (MQ=255)
                                                                                                                        aTCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAAt                               >  1:622586/1‑150 (MQ=255)
                                                                                                                           ggACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                            >  2:1678573/1‑150 (MQ=255)
                                                                                                                            gACTCTTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGccc                           >  1:3575470/1‑150 (MQ=255)
                                                                                                                             aCTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                          >  1:294125/1‑150 (MQ=255)
                                                                                                                               tctTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGc                        <  2:2859257/150‑1 (MQ=255)
                                                                                                                               tctTCGGCGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGc                        >  1:3521979/1‑150 (MQ=255)
                                                                                                                                    ggCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTc                   >  1:3464233/1‑150 (MQ=255)
                                                                                                                                      cGTCAATGTTGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCAt                 >  1:1950388/1‑150 (MQ=255)
                                                                                                                                        tCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAg               <  1:1647170/150‑1 (MQ=255)
                                                                                                                                           aTGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATg            >  1:1861939/1‑150 (MQ=255)
                                                                                                                                              ttGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCa         <  1:4084773/150‑1 (MQ=255)
                                                                                                                                              ttGATACAAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCa         >  2:1870136/1‑150 (MQ=255)
                                                                                                                                                gATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACg       <  2:497323/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCGATCACCAACGGGCAGCGTTTTTGATAAGCCTGAATAACAAAACCGATGCCGTTCCAGCCTGCCAGTTCCGGCTCGAAACAGAGTTTTTCCAGCAGATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGT  >  CP009273/1073094‑1073392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑