Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,118,506 A→T 9.4% L75Q (CTA→CAA)  yceB ← lipoprotein, DUF1439 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,118,5060AT 9.4% 624.7 / 6.8 222L75Q (CTA→CAA) yceBlipoprotein, DUF1439 family
Reads supporting (aligned to +/- strand):  ref base A (97/96);  new base T (2/26);  total (99/123)
Fisher's exact test for biased strand distribution p-value = 7.77e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

GTTGTACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTG  >  CP009273/1118357‑1118655
                                                                                                                                                     |                                                                                                                                                     
gTTGTACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTa                                                                                                                                                       <  2:2688653/150‑1 (MQ=255)
gTTGTACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTa                                                                                                                                                       <  1:1946721/150‑1 (MQ=255)
  tGTACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAgg                                                                                                                                                     <  1:2035409/150‑1 (MQ=255)
    tACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGt                                                                                                                                                   >  1:1508508/1‑150 (MQ=255)
    tACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGt                                                                                                                                                   >  1:3918590/1‑150 (MQ=255)
     aCCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTa                                                                                                                                                  <  2:2013045/150‑1 (MQ=255)
        gTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTGATGTCCAGATTTGGGTCTCCGGTTAGGGTAAcc                                                                                                                                               >  1:205638/1‑150 (MQ=255)
        gTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAAcc                                                                                                                                               <  2:259539/150‑1 (MQ=255)
        gTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAAcc                                                                                                                                               <  1:1444225/150‑1 (MQ=255)
        gTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAAcc                                                                                                                                               <  1:3844645/150‑1 (MQ=255)
          cGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCtt                                                                                                                                             >  2:4731901/1‑150 (MQ=255)
          cGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCtt                                                                                                                                             <  1:4173297/150‑1 (MQ=255)
             aTCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTGCAGATTTGGGTCTCCGGTTAGGGTAACCATAtt                                                                                                                                          >  1:4492348/1‑150 (MQ=255)
             aTCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTAtt                                                                                                                                          <  2:2158269/150‑1 (MQ=255)
             aTCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTAtt                                                                                                                                          <  1:2872106/150‑1 (MQ=255)
             aTCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTACCGCACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAAACTGAtt                                                                                                                                          >  1:4492395/1‑150 (MQ=255)
              tCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTc                                                                                                                                         <  1:1583967/150‑1 (MQ=255)
                 aCCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGc                                                                                                                                      >  2:1296837/1‑150 (MQ=255)
                 aCCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGGCTCCGGTTAGGGTAACCATATTCGGc                                                                                                                                      >  1:2207652/1‑150 (MQ=255)
                 aCCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGGTAGGGTAACCATATTCGGc                                                                                                                                      >  2:1613123/1‑150 (MQ=255)
                  ccACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGct                                                                                                                                     <  1:4372454/150‑1 (MQ=255)
                  ccACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGct                                                                                                                                     <  1:2945719/150‑1 (MQ=255)
                  ccACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGct                                                                                                                                     <  1:2947662/150‑1 (MQ=255)
                      ttCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTc                                                                                                                                 <  1:4486739/150‑1 (MQ=255)
                      ttCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTc                                                                                                                                 <  2:3267089/150‑1 (MQ=255)
                      ttCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTc                                                                                                                                 <  1:3495056/150‑1 (MQ=255)
                       tCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTcg                                                                                                                                <  1:1288280/150‑1 (MQ=255)
                        ccATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTcgc                                                                                                                               <  1:2098963/150‑1 (MQ=255)
                         cATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTcgcg                                                                                                                              <  1:2210150/150‑1 (MQ=255)
                           tctcTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGGCTCCGGTTAGGGGAACCTTATTCGGCTCTTCGCGAc                                                                                                                            >  2:1686148/1‑150 (MQ=255)
                              ctTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCaa                                                                                                                         <  2:1581018/150‑1 (MQ=255)
                                ttCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAAtt                                                                                                                       <  2:3376448/150‑1 (MQ=255)
                                ttCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAAtt                                                                                                                       <  2:2082443/150‑1 (MQ=255)
                                  cAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTg                                                                                                                     <  2:2472142/150‑1 (MQ=255)
                                  cAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTg                                                                                                                     <  1:2197405/150‑1 (MQ=255)
                                  cAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATTACTACCGAACAGGGAGTTCCTGTCCAGATTTGGGGCTCCGGGTAGGGTAACCATATTAGGCTCTTCGCGACCAATTTg                                                                                                                     >  1:566910/1‑150 (MQ=255)
                                      aaGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTAGGCTCTTCGCGACCAATTTGGCTg                                                                                                                 >  2:1490932/1‑150 (MQ=255)
                                        gATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGt                                                                                                               <  1:2732546/150‑1 (MQ=255)
                                        gATCGCACCTTTGTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGGTTCATGGTCGCGTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGt                                                                                                               <  2:2531047/150‑1 (MQ=255)
                                             cACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCATATTCGGCTCTTCGCGACCAAATTGGCTGGTCAGGt                                                                                                          >  1:4350630/1‑150 (MQ=255)
                                                  ttttCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTGTTACTAAAGAACAGGGAGTTAATGTACAGATTTGCGTCTCCGGTTATGGTAACCATATTAGGCTATTAGCGACCAATTTTTCTGGTGAGGttttta                                                                                                     >  2:3612594/1‑147 (MQ=255)
                                                  ttttCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTc                                                                                                     <  2:4220718/150‑1 (MQ=255)
                                                     tCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTTGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGa                                                                                                  >  1:729362/1‑150 (MQ=255)
                                                     tCTTGATCACACACGGGCAGCGCTTTCAGCTTCAGGTTCCGGGTCGCTTTCTGACTACCGACCCGGGAGTTCATGTCCAGCTGGGCGTCTCCGGTTAGGGGAACCTTAGTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGa                                                                                                  <  1:370020/150‑1 (MQ=255)
                                                      cTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGaa                                                                                                 >  2:2330155/1‑150 (MQ=255)
                                                          aTCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAat                                                                                             <  2:2648951/150‑1 (MQ=255)
                                                            cAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAatat                                                                                           <  2:2666661/150‑1 (MQ=255)
                                                             aaaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATg                                                                                          <  1:4041399/150‑1 (MQ=255)
                                                             aaaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATg                                                                                          <  2:2727399/150‑1 (MQ=255)
                                                             aaaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTAGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTAAGAACAATATg                                                                                          >  2:4084563/1‑150 (MQ=255)
                                                             aaaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCATATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACaaaaag                                                                                          >  1:1154209/1‑146 (MQ=255)
                                                             aaaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACGACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATg                                                                                          <  2:2727277/150‑1 (MQ=255)
                                                             aaaCACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACAACCCAACACGGGGTTCATGTCCAGATTGGCGTCTCCCGGTAGGGTAACCAAATTACGCGCGTAGCGCCCAAATTTGCGGGTCCTGTTTTTAAGAAAAATATg                                                                                          >  1:2513465/1‑150 (MQ=255)
                                                                  cGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTAATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGt                                                                                     >  2:1430009/1‑150 (MQ=255)
                                                                  cGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTATGACAAACGAACAGGGAGTTCATGTCCAGATTTGCGTCTCCGGTTATGGTAACCTTATTAGGCTCGTGGCGTCCAAATTTGCTGGTCAGGTTTGTGAGAACAAAATGGGCGt                                                                                     >  2:406322/1‑150 (MQ=255)
                                                                   gggCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTc                                                                                    <  1:256808/150‑1 (MQ=255)
                                                                     gCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAg                                                                                  <  1:2175097/150‑1 (MQ=255)
                                                                       aGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGcc                                                                                >  2:149575/1‑150 (MQ=255)
                                                                       aGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACAACCGAACCGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCCGGTTTGTCAGAACAATATGGGCGTCAGcc                                                                                >  2:151365/1‑150 (MQ=255)
                                                                         cgcTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCAc                                                                              <  1:1426027/150‑1 (MQ=255)
                                                                                 gCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGCGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCgggga                                                                      >  2:3803059/1‑148 (MQ=255)
                                                                                 gCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTTGCGTCTCCGGTTAGGGTAAACTTATTCGGCTCTTCGCGACCAAATTGGCTGGTCAGGTTTGTAAGAACAATATGGGCGTCAGCCACGCCGGGTa                                                                      >  2:3450204/1‑150 (MQ=255)
                                                                                  cTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTTGGGTAACATTAGTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTaa                                                                     <  2:674399/150‑1 (MQ=255)
                                                                                    tCAGTTTCATGGTCGCTTTCTGACTACCGAACTGGGTGTTCTTGTCCTGTTTGGGGTCTCCGGTTTGGTTAACCTTATTCGGCTCTTCGCGACCATTTTGGGTGGTCTGGTTTGTCATAACAATATGGGCGTCAGCCACGCCGGGTAAAc                                                                   <  2:3837267/150‑1 (MQ=255)
                                                                                       gTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTGCAGATTGGCGTCTCCGGTTAGGGTAAACTTATTCGGCTCTTCGCGACCAATTTGGCTTGTGTGGTTTGTGAGTAACATATTGGGGTCAGCCACGCCGGGTAAACCGa                                                                >  2:3061820/1‑150 (MQ=255)
                                                                                       gTTTCATGGTCGCTTTCAGACTAACGAACAGGGAGTTCATGTACAGATTGGCGTCTCCGGGTAGGGTAAACATATTAGGCTCTTAGCGACCAATTTTGCTGGTCAGGTTTGTCAGAACAATATTGGCGTCAGCCACCCCCGGTAAACCGa                                                                >  2:1332597/1‑150 (MQ=255)
                                                                                         ttCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGata                                                              <  2:3918590/150‑1 (MQ=255)
                                                                                          tcttGGTCGCTTTCTGACGACCGAACGGGGAGTTCTTGTCCAGATTGGGGTCTCCGGTTTGGGTAACCTTTTTCGGCTCTTCGCGACCATTTTGGCTGGTCCGGTTTTTACGAACATTATGGGCGTCAGCCACGCCGGGTAAACCGatat                                                             <  2:1217119/147‑1 (MQ=255)
                                                                                          tCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGatat                                                             <  2:1977236/150‑1 (MQ=255)
                                                                                            aTGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCt                                                           <  2:797838/150‑1 (MQ=255)
                                                                                              ggTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCttt                                                         >  2:4231856/1‑150 (MQ=255)
                                                                                               gTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtttt                                                        >  1:502013/1‑150 (MQ=255)
                                                                                               gTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtttt                                                        >  1:501020/1‑150 (MQ=255)
                                                                                               gTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCtttt                                                        >  1:4415232/1‑150 (MQ=255)
                                                                                                  gatttCTTACTACCGAACCGGTAGTTAATGTCCATATTGGCGTCTCCGGTTTGGTTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGTTTTGTCAGAAAAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTgag                                                     <  1:3142068/148‑1 (MQ=255)
                                                                                                     ttCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaaa                                                  >  1:1650792/1‑150 (MQ=255)
                                                                                                       cTGACTACCGAACCGGGTGTTCAGGTCCTGTTTGGGGTCTCGGGTTTGGGTAACCTTTTTCGGCTCTTCGCGACCATTTTGGCTGTTCTGTTTTTTCACAACAATTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatt                                                <  1:3413683/150‑1 (MQ=255)
                                                                                                        tGACTACCGAACCGGGTGTTCATGTCCAGATTGGCGTCTCCGGTTCGGGTAACCTTATGCGGCTCTTCGCGACCAATTTGGCTGGTCTGGTTTGACAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatta                                               <  2:3516268/150‑1 (MQ=255)
                                                                                                         gACTACCGAACCGGGTGTTCTTGTCCTGATGGGCGTCGCGGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTGGGCTGGTCAGGTTTGTCATAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattat                                              <  2:513641/150‑1 (MQ=255)
                                                                                                         gACTACCGAACAGGGAGTTCTTGTCCAGTTTGGCGTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCGACAATTTTGGCTGGTCTGGTTTGTCAGAACAATAGGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattat                                              <  2:2953603/150‑1 (MQ=255)
                                                                                                         gACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattat                                              >  2:883603/1‑150 (MQ=255)
                                                                                                         gACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAattat                                              >  1:3710160/1‑150 (MQ=255)
                                                                                                           cTACCTAACCGGGTGTTCTTGTCCAGTTTGGCGTCTCGGGTTTGGGTAACCTTATTCGGCTCTTCGCGACCATTTTGGCTGGTCTGGTTTGTCAGAACAATTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAAttatta                                            <  2:4442826/150‑1 (MQ=255)
                                                                                                           cTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAAttatta                                            >  1:4241746/1‑150 (MQ=255)
                                                                                                            tACCGACCTGGGTTTTCTTGTCCTGTTGGGCGTCTCGGGTTTGGGTAACCTTTTTCGGCTCTTCGCGACAATTTGGGGTGGTCTGTTTTGTCACAACAATTGGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                           <  1:3743648/150‑1 (MQ=255)
                                                                                                            tACCGACCTGGGTGTTCTTGTCCTTATGGGCGTCGGGGTTTTGGTTAACCTTATTCGGCTGTTCGCGACAATTTTGGGTGGTCTGTTTTGTCATAACAATCGGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                           <  1:235549/150‑1 (MQ=255)
                                                                                                                  agtgggTGTTCTTGTCCTGTTTGGCGTCTCGGGTTTGGTTAACTTTTTGGGGCTCTTCGCCACATTTTTGGCTGTTCTTTTTTGACATAACATTCTGGGCGACACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCg                                     <  1:2467878/147‑1 (MQ=255)
                                                                                                                   cAGGGAGTTCATGTCCAGATTGTAGTCTCCGTTTAGTGAAACATTATTCGGCTCTTCGCGACCAATTAGGCTGGTCAGGTTTGTCAGAACAAAATCGTCGACAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGc                                    >  1:4780017/1‑150 (MQ=255)
                                                                                                                     gggAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCaa                                  >  2:1768940/1‑150 (MQ=255)
                                                                                                                              tGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGt                         >  2:3142958/1‑150 (MQ=255)
                                                                                                                                     aTTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTc                  <  2:2363623/150‑1 (MQ=255)
                                                                                                                                       gggCGTCTCCGGTTTGGGTAACCTTTTGCGGCTGTTCGCGACCATTTGGGCTGGTCTGTTTTTTCAGAACAATATGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCtt                <  2:1740701/149‑1 (MQ=255)
                                                                                                                                        ggCGTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTg               <  2:4357415/150‑1 (MQ=255)
                                                                                                                                           gTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc            >  2:3225625/1‑150 (MQ=255)
                                                                                                                                           gTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc            >  1:699304/1‑150 (MQ=255)
                                                                                                                                            tctcCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCATTTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCg           <  2:1159049/150‑1 (MQ=255)
                                                                                                                                                  gTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg     >  1:4338809/1‑150 (MQ=255)
                                                                                                                                                   ttAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATgg    >  2:2799785/1‑150 (MQ=255)
                                                                                                                                                    tAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgt   >  1:1174145/1‑150 (MQ=255)
                                                                                                                                                    tAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgt   >  1:4686228/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGTACCGTCGCATCGACCACTTCCATCTCTTTCAGGAAGATCGCACCTTTTTCTTTATCAAACACGGGCAGCGCTTTCAGCTTCAGTTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTTGGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTG  >  CP009273/1118357‑1118655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑