Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,152,278 G→T 90.9% E16* (GAA→TAA)  ycfH → putative DNase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,152,2780GT90.9% 857.5 / 71.6 294E16* (GAA→TAA) ycfHputative DNase
Reads supporting (aligned to +/- strand):  ref base G (10/17);  new base T (132/135);  total (142/152)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

AGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGT  >  CP009273/1152129‑1152424
                                                                                                                                                     |                                                                                                                                                  
  gCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTaa                                                                                                                                                  <  1:3916603/150‑1 (MQ=255)
    aTCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATc                                                                                                                                                <  2:1376460/150‑1 (MQ=255)
      cAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCtt                                                                                                                                              <  1:2782043/150‑1 (MQ=255)
      cAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCtt                                                                                                                                              <  1:2610067/150‑1 (MQ=255)
      cAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCtt                                                                                                                                              <  2:1211378/150‑1 (MQ=255)
       aaCCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCttt                                                                                                                                             <  2:3056345/150‑1 (MQ=255)
           gcgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCAt                                                                                                                                         <  1:957406/150‑1 (MQ=255)
           gcgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCAt                                                                                                                                         <  1:1401511/150‑1 (MQ=255)
            cgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATa                                                                                                                                        <  2:3119613/150‑1 (MQ=255)
             gAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTATTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATaa                                                                                                                                       >  1:4066735/1‑150 (MQ=255)
             gAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATaa                                                                                                                                       >  1:3332704/1‑150 (MQ=255)
                 ttCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGAc                                                                                                                                   >  2:780588/1‑150 (MQ=255)
                  tctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCCTCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACg                                                                                                                                  >  1:106711/1‑150 (MQ=255)
                  tctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATTGTCTTGATTATTAATATTTGAATAAGGaag                                                                                                                                  >  1:4123392/1‑148 (MQ=255)
                    tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTg                                                                                                                                <  1:833442/150‑1 (MQ=255)
                    tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTg                                                                                                                                >  2:253891/1‑150 (MQ=255)
                    tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCAGAAGGACGTg                                                                                                                                >  2:2969887/1‑150 (MQ=255)
                     catcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTgg                                                                                                                               >  1:4500959/1‑150 (MQ=255)
                       tcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGAt                                                                                                                             <  1:2652759/150‑1 (MQ=255)
                        caCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATg                                                                                                                            >  2:2094259/1‑150 (MQ=255)
                           cGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACg                                                                                                                         >  1:2197159/1‑150 (MQ=255)
                             aTCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCTCACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGtt                                                                                                                       >  1:3751535/1‑150 (MQ=255)
                             aTCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGtt                                                                                                                       >  2:2039471/1‑150 (MQ=255)
                             aTCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGtt                                                                                                                       >  2:43912/1‑150 (MQ=255)
                                ttttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTg                                                                                                                    <  1:1440976/150‑1 (MQ=255)
                                   tACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCg                                                                                                                 <  1:1940858/150‑1 (MQ=255)
                                     cTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGaa                                                                                                               <  1:3972528/150‑1 (MQ=255)
                                      tGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGaaa                                                                                                              <  2:3690179/150‑1 (MQ=255)
                                      tGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGaaa                                                                                                              >  1:3473863/1‑150 (MQ=255)
                                      tGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGaaa                                                                                                              >  1:1068617/1‑150 (MQ=255)
                                      tGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGaaa                                                                                                              >  2:636110/1‑150 (MQ=255)
                                       gCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAg                                                                                                             <  1:3880828/150‑1 (MQ=255)
                                        cGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgc                                                                                                            <  2:2060008/150‑1 (MQ=255)
                                         gTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgcc                                                                                                           <  2:1845461/150‑1 (MQ=255)
                                         gTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgcc                                                                                                           <  2:1531887/150‑1 (MQ=255)
                                          tATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgccg                                                                                                          >  1:734739/1‑150 (MQ=255)
                                           aTTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgccgc                                                                                                         >  1:3183782/1‑150 (MQ=255)
                                            ttGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgccgcc                                                                                                        <  2:3158270/150‑1 (MQ=255)
                                                 cATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACCTCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCAcg                                                                                                   >  1:749244/1‑150 (MQ=255)
                                                  aTTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCACAcgc                                                                                                  >  2:3765739/1‑150 (MQ=255)
                                                      ccTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAtg                                                                                              >  2:1235143/1‑150 (MQ=255)
                                                        tGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAtgtg                                                                                            >  2:326864/1‑150 (MQ=255)
                                                        tGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAtgtg                                                                                            <  2:3720127/150‑1 (MQ=255)
                                                          cAACCGGGCGTTGTTCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGAGAAAGCCGCCGCACGCGATGTGaa                                                                                          <  2:2366761/150‑1 (MQ=255)
                                                          cAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaa                                                                                          <  1:3615561/150‑1 (MQ=255)
                                                           aaCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                         >  2:1718782/1‑150 (MQ=255)
                                                           aaCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGGCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                         >  2:3918856/1‑150 (MQ=255)
                                                           aaCCGGGCGTTGTGCTACCGGTTCCTCATATTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                         <  1:161043/150‑1 (MQ=255)
                                                             ccGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtt                                                                                       <  1:257413/150‑1 (MQ=255)
                                                                  cGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTc                                                                                  >  2:1577474/1‑150 (MQ=255)
                                                                     tgtgCTACCTGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTgg                                                                               <  2:2958966/150‑1 (MQ=255)
                                                                       tgCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCa                                                                             <  2:2957048/150‑1 (MQ=255)
                                                                           aCCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCg                                                                         <  2:4083681/150‑1 (MQ=255)
                                                                            ccGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGc                                                                        >  1:1233561/1‑150 (MQ=255)
                                                                             cGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGcc                                                                       <  2:1146262/150‑1 (MQ=255)
                                                                             cGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGcc                                                                       >  2:505723/1‑150 (MQ=255)
                                                                              gggtCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCca                                                                      <  1:235026/147‑1 (MQ=255)
                                                                              ggTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCca                                                                      >  2:2247363/1‑150 (MQ=255)
                                                                               gTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCcac                                                                     >  2:3617133/1‑150 (MQ=255)
                                                                                  ccTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaac                                                                  >  1:3101060/1‑150 (MQ=255)
                                                                                   cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaaca                                                                 >  2:1387274/1‑150 (MQ=255)
                                                                                      aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATACGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTa                                                              <  2:16239/150‑1 (MQ=255)
                                                                                       tCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTAc                                                             <  1:1950859/150‑1 (MQ=255)
                                                                                        cTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTAcc                                                            <  2:4036755/150‑1 (MQ=255)
                                                                                          ttAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCgg                                                          >  2:788026/1‑150 (MQ=255)
                                                                                           tAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCggg                                                         <  2:3473863/150‑1 (MQ=255)
                                                                                               agagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAt                                                     <  2:832992/150‑1 (MQ=255)
                                                                                                gagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAtt                                                    >  2:1943753/1‑150 (MQ=255)
                                                                                                gagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAtt                                                    >  2:1296178/1‑150 (MQ=255)
                                                                                                gagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAtt                                                    <  1:1754222/150‑1 (MQ=255)
                                                                                                  gaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTa                                                  >  2:4635259/1‑150 (MQ=255)
                                                                                                   aCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTAc                                                 >  1:2087471/1‑150 (MQ=255)
                                                                                                     atcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACat                                               >  1:2957656/1‑150 (MQ=255)
                                                                                                          gTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCg                                          <  1:464543/150‑1 (MQ=255)
                                                                                                            ttttAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCggg                                        <  1:1123758/150‑1 (MQ=255)
                                                                                                                   cGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGt                                 <  1:3963398/150‑1 (MQ=255)
                                                                                                                    gACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTa                                >  2:2753722/1‑150 (MQ=255)
                                                                                                                    gACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTa                                >  2:2468446/1‑150 (MQ=255)
                                                                                                                    gACGCACACTGCCATCTCGATGGTCTGGATTATTAATCTGTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTa                                <  2:4551283/150‑1 (MQ=255)
                                                                                                                       tCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGc                             <  1:2204354/150‑1 (MQ=255)
                                                                                                                       tCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGc                             >  2:3203997/1‑150 (MQ=255)
                                                                                                                        cacaCTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCg                            <  1:782235/150‑1 (MQ=255)
                                                                                                                         acacTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGa                           >  2:1178733/1‑150 (MQ=255)
                                                                                                                         acacTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGa                           <  2:2200390/150‑1 (MQ=255)
                                                                                                                              gCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTg                      <  1:670946/150‑1 (MQ=255)
                                                                                                                               ccATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGa                     <  1:472159/150‑1 (MQ=255)
                                                                                                                               ccATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGa                     <  1:636110/150‑1 (MQ=255)
                                                                                                                               ccATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGa                     >  2:3402460/1‑150 (MQ=255)
                                                                                                                                cATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGAc                    >  2:4363420/1‑150 (MQ=255)
                                                                                                                                 aTCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACa                   <  2:1902681/150‑1 (MQ=255)
                                                                                                                                      gATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtc              >  1:2239300/1‑150 (MQ=255)
                                                                                                                                       aTGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcg             >  2:3806623/1‑150 (MQ=255)
                                                                                                                                       aTGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcg             >  1:4383511/1‑150 (MQ=255)
                                                                                                                                        tGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcgt            >  1:1258654/1‑150 (MQ=255)
                                                                                                                                        tGGTCTGGAGTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcgt            <  2:4137720/150‑1 (MQ=255)
                                                                                                                                         ggTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTa           >  1:4249040/1‑150 (MQ=255)
                                                                                                                                             tGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtttt       >  2:2710291/1‑150 (MQ=255)
                                                                                                                                                attattAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt    <  1:3267509/150‑1 (MQ=255)
                                                                                                                                                 ttattaATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtg   >  1:4780919/1‑150 (MQ=255)
                                                                                                                                                  tATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtgt  >  2:3438916/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGT  >  CP009273/1152129‑1152424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑