Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,251,120 T→A 32.4% T97S (ACC→TCC)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,251,1200TA32.4% 364.2 / 260.1 253T97S (ACC→TCC) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base T (74/96);  new base A (41/41);  total (116/137)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

AGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATCAG  >  CP009273/1250971‑1251269
                                                                                                                                                     |                                                                                                                                                     
aGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGt                                                                                                                                                       <  2:1029468/150‑1 (MQ=255)
aGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGt                                                                                                                                                       >  2:1249878/1‑150 (MQ=255)
aGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGt                                                                                                                                                       >  2:3061590/1‑150 (MQ=255)
  cATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGa                                                                                                                                                     >  2:4430009/1‑150 (MQ=255)
   aTGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAc                                                                                                                                                    <  1:887551/150‑1 (MQ=255)
    tGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGAca                                                                                                                                                   <  2:2322062/150‑1 (MQ=255)
      ggTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGAcatc                                                                                                                                                 >  1:165987/1‑150 (MQ=255)
         ataGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCAtt                                                                                                                                              <  1:3514843/150‑1 (MQ=255)
         ataGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCAtt                                                                                                                                              <  1:2831541/150‑1 (MQ=255)
             tCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTAccc                                                                                                                                          <  2:73559/150‑1 (MQ=255)
                ccAATTAAGTTAATGCCGAGGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAgg                                                                                                                                       <  2:4691625/150‑1 (MQ=255)
                 cAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGt                                                                                                                                      >  2:4160106/1‑150 (MQ=255)
                  aaTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGtt                                                                                                                                     <  2:3879913/150‑1 (MQ=255)
                  aaTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGtt                                                                                                                                     >  2:387133/1‑150 (MQ=255)
                   aTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTg                                                                                                                                    <  2:3414984/150‑1 (MQ=255)
                   aTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTg                                                                                                                                    <  2:1638680/150‑1 (MQ=255)
                   aTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTg                                                                                                                                    >  1:2602873/1‑150 (MQ=255)
                    ttAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGa                                                                                                                                   <  1:3950121/150‑1 (MQ=255)
                    ttAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGa                                                                                                                                   <  1:1077896/150‑1 (MQ=255)
                      aaGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTAACCAGGTTGata                                                                                                                                 >  1:1018774/1‑150 (MQ=255)
                        gTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATAtt                                                                                                                               <  1:1440141/150‑1 (MQ=255)
                         ttAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTg                                                                                                                              <  1:3280594/150‑1 (MQ=255)
                         ttAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTACTTTTGCATATCCAGAGGCGTCGTTTAAATTGACGGTGACATCATTACACAGGTTGATATTg                                                                                                                              >  2:973605/1‑150 (MQ=255)
                             tGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAg                                                                                                                          <  1:2081434/150‑1 (MQ=255)
                               ccGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGGTGATATTGCCAGGc                                                                                                                        >  1:3374084/1‑150 (MQ=255)
                                cGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCg                                                                                                                       >  1:2334623/1‑150 (MQ=255)
                                  aTGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCgtg                                                                                                                     <  1:3334725/150‑1 (MQ=255)
                                    ggCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGTCAACATTACCCAGGTTGATATTGCCAGGCGGGtt                                                                                                                   >  1:4501558/1‑150 (MQ=255)
                                     gCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGttt                                                                                                                  >  1:2139419/1‑150 (MQ=255)
                                      cAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTc                                                                                                                 <  1:4732340/150‑1 (MQ=255)
                                       agagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTcc                                                                                                                >  2:2500337/1‑150 (MQ=255)
                                        gagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCct                                                                                                               >  1:629810/1‑150 (MQ=255)
                                            tttGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgctg                                                                                                           <  2:4417747/150‑1 (MQ=255)
                                               gACCAACAACATCTACTGTGAGTCGGTTAGCTGTCGGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTgggg                                                                                                        >  2:3146258/1‑150 (MQ=255)
                                                aCCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGt                                                                                                       <  1:3651581/150‑1 (MQ=255)
                                                    acaacaTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCAtt                                                                                                   <  1:3728086/150‑1 (MQ=255)
                                                     caacaTCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTAATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTa                                                                                                  >  2:1572078/1‑150 (MQ=255)
                                                           cTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTTCCAGGCGTGTTTCCTGCTGGGGTCATTACACCAt                                                                                            >  2:4271214/1‑150 (MQ=255)
                                                            tACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATa                                                                                           <  2:539607/150‑1 (MQ=255)
                                                               tgtgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATagag                                                                                        >  2:1775649/1‑150 (MQ=255)
                                                                 tgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGAAGGAGACATCATTACCCAGGTTGATATTTCCAGGCGTGTTTCCTGCTGGGGTCATTAAACCATAGAGGt                                                                                      >  1:3866927/1‑150 (MQ=255)
                                                                    gTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTc                                                                                   <  1:4270468/150‑1 (MQ=255)
                                                                        gTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGa                                                                               <  1:3132859/150‑1 (MQ=255)
                                                                           aGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGAGTCCTTTTGCATATCCAGAGGCGTCGGTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATgg                                                                            <  2:3699688/150‑1 (MQ=255)
                                                                              tGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATc                                                                         <  1:76942/150‑1 (MQ=255)
                                                                              tGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGAAAATGACGGAGACATCAATAACCACGTTGATATTGCCAGGCGTGTTTACTGCTGGGGTCATTACACCAAATAGGTTTCCTGATGGAta                                                                         >  1:407610/1‑149 (MQ=255)
                                                                               gTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcg                                                                        >  2:4140124/1‑150 (MQ=255)
                                                                               gTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcg                                                                        <  2:2186327/150‑1 (MQ=255)
                                                                                tCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcgg                                                                       <  2:4577764/150‑1 (MQ=255)
                                                                                tCAGGGAGCTGTTTTTGCACTGAATTATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCTGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcgg                                                                       <  1:3228304/150‑1 (MQ=255)
                                                                                 cAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcggc                                                                      <  1:1787329/150‑1 (MQ=255)
                                                                                  aGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcggcg                                                                     <  1:3493021/150‑1 (MQ=255)
                                                                                   gggAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcggcgg                                                                    <  1:673823/150‑1 (MQ=255)
                                                                                   gggAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcggcgg                                                                    <  1:675654/150‑1 (MQ=255)
                                                                                        cTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATATAGGTTTCCTGATGGATCGGCGGTAtct                                                               >  2:3581660/1‑150 (MQ=255)
                                                                                        cTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTAtct                                                               >  1:3861220/1‑150 (MQ=255)
                                                                                         tGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTAtctc                                                              >  2:4765730/1‑150 (MQ=255)
                                                                                          gTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTcc                                                             >  1:1382992/1‑150 (MQ=255)
                                                                                             tttGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGt                                                          <  1:3118399/150‑1 (MQ=255)
                                                                                              ttGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATTTCCTGTa                                                         >  2:2920923/1‑150 (MQ=255)
                                                                                              ttGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTa                                                         <  1:1215536/150‑1 (MQ=255)
                                                                                               tGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTaa                                                        >  1:4180696/1‑150 (MQ=255)
                                                                                               tGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTaa                                                        >  1:3717628/1‑150 (MQ=255)
                                                                                                  ccTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTc                                                     <  1:4202035/150‑1 (MQ=255)
                                                                                                   cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                    <  1:2170521/150‑1 (MQ=255)
                                                                                                   cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                    <  2:3776743/150‑1 (MQ=255)
                                                                                                   cTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCt                                                    <  2:1367795/150‑1 (MQ=255)
                                                                                                     gAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGt                                                  <  1:4020712/150‑1 (MQ=255)
                                                                                                       ataatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCa                                                >  1:2990666/1‑150 (MQ=255)
                                                                                                       ataatGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTAAACCATAGAGGTTTACTGATGGATAGGCGGTATCTCCTGTAATTCTGtaa                                                >  1:1587068/1‑148 (MQ=255)
                                                                                                           tGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTATGTCACcat                                            >  2:4068823/1‑150 (MQ=255)
                                                                                                           tGAGTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACcat                                            <  2:3316347/150‑1 (MQ=255)
                                                                                                                ccTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTa                                       <  2:1684164/150‑1 (MQ=255)
                                                                                                                ccTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCAAAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTa                                       >  1:900337/1‑150 (MQ=255)
                                                                                                                    ttGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAg                                   <  2:355225/150‑1 (MQ=255)
                                                                                                                    ttGCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAg                                   <  2:2937902/150‑1 (MQ=255)
                                                                                                                      gCATATCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTa                                 <  2:196947/150‑1 (MQ=255)
                                                                                                                        atatCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAat                               >  1:4546759/1‑150 (MQ=255)
                                                                                                                          atCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAatat                             >  2:6625/1‑150 (MQ=255)
                                                                                                                           tCCAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGTCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATAtt                            >  2:4432920/1‑150 (MQ=255)
                                                                                                                            ccAGAGGCGTCGTGGACATTGACGGTGACATCATTCCCCAGGTTGAGCTTGCCCGGCGGGTTTCCTGCTGGGGTCATTACACCCTCGAGGTTTCCTGATGGAGCGGCGGTCTCTCCTGTAATTCTGTCCCCATCCTGAATAGTAATATTg                           >  2:3272205/1‑150 (MQ=255)
                                                                                                                             cAGAGGCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTgg                          >  2:2754401/1‑150 (MQ=255)
                                                                                                                                 ggCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTTCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTTATTGATCGGCGGTATATACTGTAATTATTTCACCAACATTAATATTAAAATTGGTGcc                      >  1:2656991/1‑150 (MQ=255)
                                                                                                                                 ggCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGcc                      >  2:281896/1‑150 (MQ=255)
                                                                                                                                 ggCGTCGTTGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGcc                      >  2:398265/1‑150 (MQ=255)
                                                                                                                                    gtcgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCata                   >  2:3280700/1‑150 (MQ=255)
                                                                                                                                     tcgtTGACATTGACGGTGACATCATTACCCAGGTTGATAGTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCatat                  <  2:2259029/150‑1 (MQ=255)
                                                                                                                                      cgtTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATAtt                 >  2:3690135/1‑150 (MQ=255)
                                                                                                                                      cgtTGACATTGACGGTGACATCATTACCCAGGTTGATAGTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATAtt                 <  2:4501558/150‑1 (MQ=255)
                                                                                                                                        ttGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGa               <  1:3941387/150‑1 (MQ=255)
                                                                                                                                         tGACATTGACGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGAc              <  2:2335504/150‑1 (MQ=255)
                                                                                                                                          gACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACt             <  1:387133/150‑1 (MQ=255)
                                                                                                                                             aTTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTcc          >  2:4369862/1‑150 (MQ=255)
                                                                                                                                              ttGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCt         <  2:2099667/150‑1 (MQ=255)
                                                                                                                                               tGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTg        <  1:1124765/150‑1 (MQ=255)
                                                                                                                                                  cGGAGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTAt     >  2:1814519/1‑150 (MQ=255)
                                                                                                                                                    gTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATca   <  2:3374084/150‑1 (MQ=255)
                                                                                                                                                    gTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATca   >  2:1173581/1‑150 (MQ=255)
                                                                                                                                                    gTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATca   <  1:4146998/150‑1 (MQ=255)
                                                                                                                                                     tGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATcag  <  1:2910785/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCTGTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATCAG  >  CP009273/1250971‑1251269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑