Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,529,506 C→T 31.6% G204S (GGC→AGC)  yddE ← PhzC‑PhzF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,529,5060CT31.6% 554.5 / inf 353G204S (GGC→AGC) yddEPhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base C (118/123);  new base T (55/56);  total (174/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

GCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA  >  CP009273/1529357‑1529655
                                                                                                                                                     |                                                                                                                                                     
gCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGcc                                                                                                                                                       >  1:1077761/1‑150 (MQ=255)
gCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGcc                                                                                                                                                       >  2:1871510/1‑150 (MQ=255)
 ccTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCt                                                                                                                                                      <  1:4084862/150‑1 (MQ=255)
 ccTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCt                                                                                                                                                      >  1:3542984/1‑150 (MQ=255)
    ttAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTggg                                                                                                                                                   >  2:1125593/1‑150 (MQ=255)
      aaCACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCCCCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCg                                                                                                                                                 <  1:1444140/150‑1 (MQ=255)
      aaCACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCg                                                                                                                                                 >  1:442439/1‑150 (MQ=255)
      aaCACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCg                                                                                                                                                 <  2:4064460/150‑1 (MQ=255)
       acacGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCgg                                                                                                                                                >  2:4195262/1‑150 (MQ=255)
        cacGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGa                                                                                                                                               >  1:2483587/1‑150 (MQ=255)
          cGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATc                                                                                                                                             >  1:3166741/1‑150 (MQ=255)
           gCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCt                                                                                                                                            <  1:335597/150‑1 (MQ=255)
            cAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTg                                                                                                                                           >  2:1687470/1‑150 (MQ=255)
                acGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAAc                                                                                                                                       <  2:1616037/150‑1 (MQ=255)
                 cGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACg                                                                                                                                      >  2:4037038/1‑150 (MQ=255)
                    tGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGga                                                                                                                                   <  1:2903497/150‑1 (MQ=255)
                     gCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGTGCCATTGGCATTGCCAGTCACCGGATCATCCACAATACCAATCGCAGGCGAGAACATGCGACCATCTGTTTCGTGTTTGCCTGGGCGGATCTGGAACGGgaa                                                                                                                                  >  1:3319018/1‑150 (MQ=255)
                      ccATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaag                                                                                                                                 >  2:4736534/1‑150 (MQ=255)
                       cATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGgaaga                                                                                                                                >  2:4775569/1‑150 (MQ=255)
                       cATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaaga                                                                                                                                >  1:4734183/1‑150 (MQ=255)
                       cATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaaga                                                                                                                                >  1:98496/1‑150 (MQ=255)
                        aTCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCTCCGGATCTTCCACAATCCCATTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaagaa                                                                                                                               <  2:4151654/150‑1 (MQ=255)
                        aTCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGgaagaa                                                                                                                               >  1:1937231/1‑150 (MQ=255)
                             ggggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                          <  1:421929/150‑1 (MQ=255)
                             ggggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                          <  1:457006/150‑1 (MQ=255)
                              gggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAtt                                                                                                                         <  2:2984009/150‑1 (MQ=255)
                                 cAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCa                                                                                                                      <  1:4302776/150‑1 (MQ=255)
                                 cAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCa                                                                                                                      <  1:1396444/150‑1 (MQ=255)
                                   aTACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATGCCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGc                                                                                                                    >  2:1757711/1‑150 (MQ=255)
                                    tACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGACCGGGACGAAACCATTGCAGcc                                                                                                                   >  2:2343376/1‑150 (MQ=255)
                                    tACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGcc                                                                                                                   >  2:4304590/1‑150 (MQ=255)
                                     aCGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCa                                                                                                                  >  1:3028943/1‑150 (MQ=255)
                                      cGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCaa                                                                                                                 <  1:1681594/150‑1 (MQ=255)
                                      cGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCaa                                                                                                                 <  1:3991685/150‑1 (MQ=255)
                                       gTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAAt                                                                                                                >  2:2044484/1‑150 (MQ=255)
                                         tgtgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCt                                                                                                              >  1:3295618/1‑150 (MQ=255)
                                            gATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCtttt                                                                                                           <  2:1613725/150‑1 (MQ=255)
                                             aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                          <  1:1887573/150‑1 (MQ=255)
                                                tACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCt                                                                                                       <  1:1421306/150‑1 (MQ=255)
                                                tACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCt                                                                                                       >  2:3251671/1‑150 (MQ=255)
                                                 accaaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTg                                                                                                      >  1:1034925/1‑150 (MQ=255)
                                                    aaccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATa                                                                                                   <  1:3629177/150‑1 (MQ=255)
                                                     accaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAg                                                                                                  <  2:4709521/150‑1 (MQ=255)
                                                     accaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAg                                                                                                  <  2:454684/150‑1 (MQ=255)
                                                     accaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAg                                                                                                  <  1:724899/150‑1 (MQ=255)
                                                     accaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAg                                                                                                  <  2:3896425/150‑1 (MQ=255)
                                                     accaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAg                                                                                                  <  1:3145251/150‑1 (MQ=255)
                                                      ccaTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGc                                                                                                 <  2:517825/150‑1 (MQ=255)
                                                        aTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCgg                                                                                               >  1:4275539/1‑150 (MQ=255)
                                                         tGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATCGCGGt                                                                                              >  2:3471059/1‑150 (MQ=255)
                                                           cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCa                                                                                            <  2:3813285/150‑1 (MQ=255)
                                                           cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGGCa                                                                                            >  1:2742750/1‑150 (MQ=255)
                                                            aCCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGTGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAg                                                                                           >  2:633944/1‑150 (MQ=255)
                                                                aTCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCa                                                                                       >  2:1294548/1‑150 (MQ=255)
                                                                  cGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCAtt                                                                                     >  1:2268696/1‑150 (MQ=255)
                                                                   gggCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTa                                                                                    >  2:1490936/1‑150 (MQ=255)
                                                                    ggCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTaa                                                                                   <  2:249962/150‑1 (MQ=255)
                                                                       cATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGt                                                                                <  2:2681156/150‑1 (MQ=255)
                                                                        aTTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCCTTAAGGTc                                                                               >  1:46895/1‑150 (MQ=255)
                                                                         ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                              <  1:3905282/150‑1 (MQ=255)
                                                                         ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCCGCGCATTAAGGTCa                                                                              >  1:4020355/1‑150 (MQ=255)
                                                                         ttGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                              <  2:3930282/150‑1 (MQ=255)
                                                                          tGNNATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAg                                                                             >  2:1172678/1‑150 (MQ=255)
                                                                          tGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAg                                                                             <  2:4714312/150‑1 (MQ=255)
                                                                             cATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATATGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                          <  2:2840607/150‑1 (MQ=255)
                                                                                tGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGaaa                                                                       <  1:123047/150‑1 (MQ=255)
                                                                                 gCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAg                                                                      <  1:4581932/150‑1 (MQ=255)
                                                                                  ccAGTCACCGTATCTTCCACAATACCAATCGCAGGCGAGAACATTCGACCATAGGTTTCGTTTTTGACTGGGCGGATATGTAACTGGAAGAAACCATTTCATACAATCTTTTTGCTGATATCGGTCAGCGAATTAAGGTAAGGCGaaatg                                                                     >  1:2586004/1‑148 (MQ=255)
                                                                                       cACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTc                                                                <  2:442439/150‑1 (MQ=255)
                                                                                        aCCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCg                                                               >  1:1847179/1‑150 (MQ=255)
                                                                                        aCCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCg                                                               >  2:1361000/1‑150 (MQ=255)
                                                                                        aCCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCg                                                               <  2:4358295/150‑1 (MQ=255)
                                                                                          cGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGat                                                             <  1:265450/150‑1 (MQ=255)
                                                                                          cGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGat                                                             <  1:1286473/150‑1 (MQ=255)
                                                                                           ggATCTTCCACAATACCAATCGCAGGCGAGAACATGCGGCCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGata                                                            >  2:2152434/1‑150 (MQ=255)
                                                                                            gATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGTTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGatat                                                           <  2:3279529/150‑1 (MQ=255)
                                                                                             aTCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATc                                                          >  2:3411619/1‑150 (MQ=255)
                                                                                              tCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATcc                                                         >  1:2180123/1‑150 (MQ=255)
                                                                                                  ccACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACtt                                                     <  1:3800485/150‑1 (MQ=255)
                                                                                                    acaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTcc                                                   <  1:1159791/150‑1 (MQ=255)
                                                                                                      aaTACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCgg                                                 <  1:619067/150‑1 (MQ=255)
                                                                                                       aTACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGt                                                >  2:3580653/1‑150 (MQ=255)
                                                                                                          ccAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTc                                             <  1:4351653/150‑1 (MQ=255)
                                                                                                          ccAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTc                                             <  2:824169/150‑1 (MQ=255)
                                                                                                            aaTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCATTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATACACTTCCGGTTTCAg                                           >  1:121395/1‑150 (MQ=255)
                                                                                                             aTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGt                                          <  1:3682495/150‑1 (MQ=255)
                                                                                                               cGCAGGCGAGAACATGCGACCATCGGTTTCGTGTTTGCTTGGGCGGATCGGGAACGGGAAGAAACCCATGCAGCCAATATTTTTTCTGTTATCGGTCAGCGCCTTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGGTTCAGTgg                                        >  1:884213/1‑150 (MQ=255)
                                                                                                                   ggCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatc                                    <  2:3593324/150‑1 (MQ=255)
                                                                                                                      gagaACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCCTTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                                 >  2:750721/1‑150 (MQ=255)
                                                                                                                          aCATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtt                             >  2:2316944/1‑150 (MQ=255)
                                                                                                                           cATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACttt                            <  2:192932/150‑1 (MQ=255)
                                                                                                                            aTGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACtttt                           >  1:4187916/1‑150 (MQ=255)
                                                                                                                               cGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCATTGGGATCATCACTTTTGAg                        >  2:893086/1‑150 (MQ=255)
                                                                                                                                gACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgt                       <  2:664984/150‑1 (MQ=255)
                                                                                                                                      cGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtg                 <  1:4784510/150‑1 (MQ=255)
                                                                                                                                         tttCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg              <  1:1622451/150‑1 (MQ=255)
                                                                                                                                         tttCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttg              <  1:2326421/150‑1 (MQ=255)
                                                                                                                                           tCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt            <  1:1975665/150‑1 (MQ=255)
                                                                                                                                            cGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGttgttg           <  2:19706/150‑1 (MQ=255)
                                                                                                                                                 ttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAcc      >  1:2878748/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTGAA  >  CP009273/1529357‑1529655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑