Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,615,798 C→A 5.1% F70L (TTC→TTA)  ydeE → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,615,7980CA 5.1% 879.5 / 4.2 254F70L (TTC→TTA) ydeEputative transporter
Reads supporting (aligned to +/- strand):  ref base C (77/149);  new base A (24/2);  total (103/151)
Fisher's exact test for biased strand distribution p-value = 6.93e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

ATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGCCTG  >  CP009273/1615649‑1615947
                                                                                                                                                     |                                                                                                                                                     
aTCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTc                                                                                                                                                       <  1:858766/150‑1 (MQ=255)
  cGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTTTACGCGGTTTTTGTTTACAGGCGGATAATTTAGa                                                                                                                                                     >  1:2103639/1‑150 (MQ=255)
  cGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGGTTTGGTATCCTGGCGGATAAGTTCGa                                                                                                                                                     >  2:3709786/1‑150 (MQ=255)
  cGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGGTTTGGTATACAGGCGGATAAGTTAGa                                                                                                                                                     >  1:3919031/1‑150 (MQ=255)
    gACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTGTTTTTGCTCGGTTTTGGTATACAGGCGGATAAATTAGACa                                                                                                                                                   >  1:2098401/1‑150 (MQ=255)
    gACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTGTTTTGCCGCGGTGTTGGGATACTGGCGGGTAAGTTGGACa                                                                                                                                                   >  1:1206917/1‑150 (MQ=255)
        cggcgCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGaa                                                                                                                                               <  1:1526829/150‑1 (MQ=255)
           cgcTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACg                                                                                                                                            <  2:3980811/150‑1 (MQ=255)
             cTACGCTGCCATATATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGACTCGGTTTTTGTATAATTGCGGATAATTTAGAAAAGAAAAGCt                                                                                                                                          >  2:3114660/1‑150 (MQ=255)
              tACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCta                                                                                                                                         <  2:3448149/150‑1 (MQ=255)
                 gCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTatat                                                                                                                                      <  2:217229/150‑1 (MQ=255)
                 gCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCG‑TTTTAGCCTCGGGTTTTGTATCCTGGCGGCTAAGTTAGACAACAAAAGCTatatt                                                                                                                                     >  1:2477145/1‑149 (MQ=255)
                      cATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAAAGCTATATGTTAc                                                                                                                                 >  2:1529888/1‑150 (MQ=255)
                       aTTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACt                                                                                                                                <  1:4035156/150‑1 (MQ=255)
                          tATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGc                                                                                                                             <  2:2801263/150‑1 (MQ=255)
                           aTGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTATCCTCGGTTTTTGTATACTGGCGGATAAGTTAGAAAATAAACGCTATATTTTaaaggcc                                                                                                                            >  1:3454148/1‑144 (MQ=255)
                            tGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTGTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACCCTATATGTTACAGGcca                                                                                                                           >  2:164443/1‑148 (MQ=255)
                              aCCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAAtt                                                                                                                         <  2:4435405/150‑1 (MQ=255)
                                      cTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCtt                                                                                                                 <  2:2930145/150‑1 (MQ=255)
                                      cTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCtt                                                                                                                 <  2:1223757/150‑1 (MQ=255)
                                          aGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTTGCCGAGGTTTTGGTATCCTGGGGGATAAGTTAGACAAGAAACCCGATATTTTACTGGCAATTTACGCCGTCGcc                                                                                                             >  1:1032856/1‑150 (MQ=255)
                                           gTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGCTAATTTCGACAAGAAAAGCTATATTTTAATTGCAAATAACCCCATCGccc                                                                                                            >  1:4675323/1‑149 (MQ=255)
                                                   tACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtttt                                                                                                    <  1:428252/150‑1 (MQ=255)
                                                    aCAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCGATATGTTACTTGCAATTACCGCCATAGCCAGCGGGTTTa                                                                                                   >  2:358551/1‑150 (MQ=255)
                                                      aGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTAtt                                                                                                 <  1:380749/150‑1 (MQ=255)
                                                      aGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTAtt                                                                                                 <  1:3699321/150‑1 (MQ=255)
                                                      aGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGGGTTGGTATACAGGCGGCTAAGTTCGACAAGAAACGCGATATGTTACAGGCCAATAACGCCGGCGCCCGCCGGTTTAtt                                                                                                 >  1:3011049/1‑150 (MQ=255)
                                                      aGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTGTTTAGCCGCGGGTTTGGTATCCAGGCGGAGAAGTTCGCCAAGAAACGCGATATGTTACGGGCCATTACCGCCGTCGCCACCGGTGTTAtt                                                                                                 >  2:2065671/1‑150 (MQ=255)
                                                        ccTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATACTGGCGGATTAGTTCGACAAGAAAAGCTATATTTTAATGGCAATTAACGCCATAGCCACCGGGTTTAtttg                                                                                               >  2:3302070/1‑148 (MQ=255)
                                                         cTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTGTTTAGCCTCGGTTTTGGTATACTGGCGGATAAGTTCGACAATAAACGCTATATTTTACTTGCAATTACCGCCTTCGCCAGCGGTTTTATTGcc                                                                                              >  2:1800341/1‑150 (MQ=255)
                                                           gAGTGTCGATCTACTCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCAt                                                                                            <  1:103572/150‑1 (MQ=255)
                                                              tgtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTAc                                                                                         <  1:2673249/150‑1 (MQ=255)
                                                               gtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTATCCACGGTTTTTGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTAACGCCATCGCCAGCGGTTTTATTTCCATTAca                                                                                        >  1:4337384/1‑149 (MQ=255)
                                                               gtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                                                        <  2:823959/150‑1 (MQ=255)
                                                               gtCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                                                        <  2:2881877/150‑1 (MQ=255)
                                                                tCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACtt                                                                                       <  1:1330739/150‑1 (MQ=255)
                                                                  gATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGGTCGACAAGAAACGCTATATGTTACAGGCAAATACCGCCTACGCCAGCGGTTTTATTGCCAATACTTTa                                                                                     >  2:3794843/1‑150 (MQ=255)
                                                                   aTCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAg                                                                                    <  2:2555305/150‑1 (MQ=255)
                                                                    tCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGt                                                                                   <  1:2056586/150‑1 (MQ=255)
                                                                    tCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGt                                                                                   <  2:4205822/150‑1 (MQ=255)
                                                                     cTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTg                                                                                  <  1:1985228/150‑1 (MQ=255)
                                                                        aTCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAAt                                                                               <  2:3956769/150‑1 (MQ=255)
                                                                           ggTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAAc                                                                            <  2:504857/150‑1 (MQ=255)
                                                                           ggTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAAc                                                                            <  1:4542755/150‑1 (MQ=255)
                                                                              tATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTg                                                                         <  1:1812073/150‑1 (MQ=255)
                                                                              tATGCGATGACAAGTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTg                                                                         <  1:4214691/150‑1 (MQ=255)
                                                                               aTGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGa                                                                        <  2:1256457/150‑1 (MQ=255)
                                                                                tGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGAc                                                                       <  1:1728342/150‑1 (MQ=255)
                                                                                     tGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTTTGCTGGCGGGTAAGTTCGACAAGAAAAGCTTTATTTTTATTGGAATTTACGCCGTCGCCCGCGGTTTTATTTCCATTAATTTATTTAATAACCTTAAGCTgg                                                                  >  2:4052853/1‑150 (MQ=255)
                                                                                        cAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTtg                                                               <  2:1614958/150‑1 (MQ=255)
                                                                                        cAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTtg                                                               <  1:2191385/150‑1 (MQ=255)
                                                                                         aaTTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTtgt                                                              <  1:1238437/150‑1 (MQ=255)
                                                                                         aaTTGCGCTCACTATTGGCGTCGTGTTTAGCCGCGGTTTTGGTATCCTGGCGGCTAAGTTCGACAAGAAACCCTATAAGTTAATGGCAATTAACCCCGTCGCCACCGGGTTTATTTCCATTACTTTAGTGAATAACCTGACGCTGGTtgt                                                              >  1:3182273/1‑150 (MQ=255)
                                                                                          aTTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTtgtg                                                             <  1:1628928/150‑1 (MQ=255)
                                                                                          aTTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGGTTTGGTAAACATGACTAAAACATAGACAAGAAACCCTAAATGTTACTTCCAAAAAACGCCATAGACAGCGGTGATATTGCAAATACAAAAGTGAAAAACCTTACTCAGTTtgtg                                                             >  2:4738747/1‑150 (MQ=255)
                                                                                            tGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGct                                                           >  2:4706269/1‑150 (MQ=255)
                                                                                             gcgcTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGctc                                                          <  1:307646/150‑1 (MQ=255)
                                                                                             gcgcTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGctc                                                          <  1:4749484/150‑1 (MQ=255)
                                                                                             gcgcTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGctc                                                          <  2:3339919/150‑1 (MQ=255)
                                                                                                cTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCttt                                                       <  1:1370528/150‑1 (MQ=255)
                                                                                                   aCTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCtttttt                                                    <  1:198601/150‑1 (MQ=255)
                                                                                                          gCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCa                                             <  2:3019042/150‑1 (MQ=255)
                                                                                                          gCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCa                                             <  2:1969680/150‑1 (MQ=255)
                                                                                                            gtcgtTTTTAGCCTCGGTTTTGGTATCCATGCGGATAATTTCGACAAGAAACCCGATATGTTACTGGCAATTACCCCCTTCGCCCCCGGTTTTATTGCCATTACTTTAGTGAATAACGTTAAGCTGGTTGTGCTCTTTTTTTCCCTCaat                                           >  1:141121/1‑148 (MQ=255)
                                                                                                              cgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTaa                                         >  2:214995/1‑150 (MQ=255)
                                                                                                              cgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTaa                                         >  2:214024/1‑150 (MQ=255)
                                                                                                               gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAAc                                        >  2:173004/1‑150 (MQ=255)
                                                                                                                  tttAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTgc                                     >  2:2696987/1‑150 (MQ=255)
                                                                                                                      gCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCt                                 >  2:4605076/1‑150 (MQ=255)
                                                                                                                      gCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCt                                 <  1:2220468/150‑1 (MQ=255)
                                                                                                                          cGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTc                             >  2:35541/1‑150 (MQ=255)
                                                                                                                           ggTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCt                            <  1:3347372/150‑1 (MQ=255)
                                                                                                                            gTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTg                           <  2:3010440/150‑1 (MQ=255)
                                                                                                                             ttttGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGt                          >  2:270827/1‑150 (MQ=255)
                                                                                                                             ttttGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGt                          >  2:2664156/1‑150 (MQ=255)
                                                                                                                             ttttGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGt                          <  1:2356914/150‑1 (MQ=255)
                                                                                                                               ttGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGttt                        <  1:1141353/150‑1 (MQ=255)
                                                                                                                                tGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGtttt                       >  1:598340/1‑150 (MQ=255)
                                                                                                                                 ggTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGttttt                      <  1:1875260/150‑1 (MQ=255)
                                                                                                                                 ggTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGttttt                      <  1:398590/150‑1 (MQ=255)
                                                                                                                                 ggTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGttttt                      <  2:3856492/150‑1 (MQ=255)
                                                                                                                                  gTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTg                     <  1:4737153/150‑1 (MQ=255)
                                                                                                                                   tATCCTGGCGGATAAGTTCGACAAGAATCGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGc                    <  1:1322985/150‑1 (MQ=255)
                                                                                                                                    aTCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCt                   <  2:2223740/150‑1 (MQ=255)
                                                                                                                                    aTCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCt                   >  2:204458/1‑150 (MQ=255)
                                                                                                                                     tCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTa                  <  2:3864226/150‑1 (MQ=255)
                                                                                                                                      ccTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTAc                 >  1:3607637/1‑150 (MQ=255)
                                                                                                                                         ggCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGt              <  2:322981/150‑1 (MQ=255)
                                                                                                                                          gCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTg             <  2:60694/150‑1 (MQ=255)
                                                                                                                                          gCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTg             <  1:308588/150‑1 (MQ=255)
                                                                                                                                            ggATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCt           >  2:3665300/1‑150 (MQ=255)
                                                                                                                                            ggATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCt           >  1:3042725/1‑150 (MQ=255)
                                                                                                                                                aaGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGaaa       >  2:3926035/1‑150 (MQ=255)
                                                                                                                                                aaGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGaaa       >  2:101404/1‑150 (MQ=255)
                                                                                                                                                aaGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGaaa       >  2:1208445/1‑150 (MQ=255)
                                                                                                                                                  gTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGc     >  2:2176260/1‑150 (MQ=255)
                                                                                                                                                   ttCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGcc    <  1:3319031/150‑1 (MQ=255)
                                                                                                                                                    tCGACAAGAAACGCTATATGTTACTGGTAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGCCt   <  1:3191570/150‑1 (MQ=255)
                                                                                                                                                    tCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGCCt   <  1:1717936/150‑1 (MQ=255)
                                                                                                                                                    tCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGCCt   <  2:2400667/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGCCTG  >  CP009273/1615649‑1615947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑