Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,661,763 C→A 100% P353P (CCG→CCT)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,661,7630CA96.0% 763.6 / 18.8 225P353P (CCG→CCT) mlcglucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (2/7);  new base A (103/113);  total (105/120)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 225 total aligned reads displayed.

ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCA  >  CP009273/1661616‑1661908
                                                                                                                                                   |                                                                                                                                                 
aCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAgg                                                                                                                                                 <  1:1528075/150‑1 (MQ=255)
   aaTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGaa                                                                                                                                              >  2:2560948/1‑150 (MQ=255)
   aaTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGaa                                                                                                                                              >  1:698081/1‑150 (MQ=255)
    aTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAag                                                                                                                                             >  2:4513134/1‑150 (MQ=255)
    aTCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAag                                                                                                                                             >  2:3737215/1‑150 (MQ=255)
        acaaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGa                                                                                                                                         <  1:1077091/150‑1 (MQ=255)
         caaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGat                                                                                                                                        >  1:1273361/1‑150 (MQ=255)
          aaaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGata                                                                                                                                       >  1:2479205/1‑150 (MQ=255)
           aaGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGatat                                                                                                                                      >  1:3473549/1‑150 (MQ=255)
             gAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCt                                                                                                                                    <  2:863255/150‑1 (MQ=255)
              aaCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTg                                                                                                                                   <  2:4046854/150‑1 (MQ=255)
              aaCCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTg                                                                                                                                   >  2:1690468/1‑150 (MQ=255)
                  gTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGc                                                                                                                               >  2:3222503/1‑150 (MQ=255)
                   ttATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCGCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCt                                                                                                                              <  1:1539263/150‑1 (MQ=255)
                    tataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCtt                                                                                                                             <  2:3973579/150‑1 (MQ=255)
                    tataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCtt                                                                                                                             >  2:3521675/1‑150 (MQ=255)
                     ataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCttt                                                                                                                            >  2:2564214/1‑150 (MQ=255)
                     ataCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCttt                                                                                                                            <  2:2050970/150‑1 (MQ=255)
                      taCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTa                                                                                                                           >  2:1024921/1‑150 (MQ=255)
                      taCATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTa                                                                                                                           >  1:4049830/1‑150 (MQ=255)
                         aTCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACtt                                                                                                                        >  1:1399142/1‑150 (MQ=255)
                           cgcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTaa                                                                                                                      <  2:3273619/150‑1 (MQ=255)
                            gcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAAc                                                                                                                     <  2:4236424/150‑1 (MQ=255)
                            gcgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAAc                                                                                                                     <  2:1834787/150‑1 (MQ=255)
                             cgTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACg                                                                                                                    >  1:3896853/1‑150 (MQ=255)
                              gTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACgg                                                                                                                   >  1:2614847/1‑150 (MQ=255)
                              gTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACgg                                                                                                                   >  1:2194267/1‑150 (MQ=255)
                                 ttttACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGa                                                                                                                <  2:1220558/150‑1 (MQ=255)
                                     aCCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCa                                                                                                            <  1:2017504/150‑1 (MQ=255)
                                       cAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAt                                                                                                          <  1:3772953/150‑1 (MQ=255)
                                       cAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAAt                                                                                                          <  2:2296139/150‑1 (MQ=255)
                                        aGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATc                                                                                                         >  1:1406885/1‑150 (MQ=255)
                                          tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAg                                                                                                       <  2:515309/150‑1 (MQ=255)
                                          tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAg                                                                                                       <  1:4741128/150‑1 (MQ=255)
                                          tGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAg                                                                                                       <  1:1001345/150‑1 (MQ=255)
                                             aGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAt                                                                                                    >  2:2142900/1‑150 (MQ=255)
                                             aGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAt                                                                                                    <  2:2472815/150‑1 (MQ=255)
                                               cgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttt                                                                                                  <  2:2420521/150‑1 (MQ=255)
                                               cgcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttt                                                                                                  <  2:3764265/150‑1 (MQ=255)
                                                gcCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtttt                                                                                                 <  2:2912429/150‑1 (MQ=255)
                                                      cATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTgg                                                                                           >  1:798312/1‑150 (MQ=255)
                                                       aTCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTggg                                                                                          >  1:273333/1‑150 (MQ=255)
                                                        tCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGt                                                                                         >  1:3486236/1‑150 (MQ=255)
                                                         cGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGtt                                                                                        >  1:905596/1‑150 (MQ=255)
                                                          gTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTta                                                                                       >  2:2298166/1‑150 (MQ=255)
                                                             cccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaat                                                                                    >  1:3870478/1‑150 (MQ=255)
                                                               cTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataa                                                                                  <  1:3795528/150‑1 (MQ=255)
                                                                tGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataaa                                                                                 <  1:1620067/150‑1 (MQ=255)
                                                                tGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTtaaataaa                                                                                 <  1:1217115/150‑1 (MQ=255)
                                                                 ggTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTaaataaat                                                                                >  1:564032/1‑150 (MQ=255)
                                                                 ggTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTaaataaat                                                                                <  1:30778/150‑1 (MQ=255)
                                                                  gTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAtt                                                                               <  1:3735363/150‑1 (MQ=255)
                                                                  gTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAAtt                                                                               <  2:698081/150‑1 (MQ=255)
                                                                    tAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCa                                                                             <  2:4113853/150‑1 (MQ=255)
                                                                     aGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                            <  2:3663813/150‑1 (MQ=255)
                                                                     aGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCGTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                            >  1:895875/1‑150 (MQ=255)
                                                                       aaaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCa                                                                          >  1:1180250/1‑150 (MQ=255)
                                                                        aaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCat                                                                         >  2:3196353/1‑150 (MQ=255)
                                                                           cTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgat                                                                      <  1:2447000/150‑1 (MQ=255)
                                                                            tGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCatgatg                                                                     <  1:2560948/150‑1 (MQ=255)
                                                                              aGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                                   <  1:1476605/150‑1 (MQ=255)
                                                                                    ctcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                             <  1:684913/150‑1 (MQ=255)
                                                                                     tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATg                                                            <  1:2662158/150‑1 (MQ=255)
                                                                                      cAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgc                                                           <  2:2847179/150‑1 (MQ=255)
                                                                                         cGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGcc                                                        <  2:2649943/150‑1 (MQ=255)
                                                                                          gCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGccc                                                       >  1:3590211/1‑150 (MQ=255)
                                                                                          gCTGATGTGCTGACTATACGCAGGAAGGGCCTACTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGccc                                                       >  1:4124734/1‑150 (MQ=255)
                                                                                           cTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCg                                                      >  2:1925119/1‑150 (MQ=255)
                                                                                                gtgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATg                                                 >  2:3677035/1‑150 (MQ=255)
                                                                                                 tgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgc                                                <  1:4201030/150‑1 (MQ=255)
                                                                                                 tgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgc                                                >  1:2317741/1‑150 (MQ=255)
                                                                                                 tgCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgc                                                <  1:2378172/150‑1 (MQ=255)
                                                                                                      aCTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGcc                                           >  2:4444041/1‑150 (MQ=255)
                                                                                                        tataCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCa                                         >  1:1766703/1‑150 (MQ=255)
                                                                                                         ataCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAc                                        <  1:1371260/150‑1 (MQ=255)
                                                                                                           aCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAccc                                      >  1:2104509/1‑150 (MQ=255)
                                                                                                            cGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcccc                                     <  2:358952/150‑1 (MQ=255)
                                                                                                            cGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAcccc                                     <  2:3071730/150‑1 (MQ=255)
                                                                                                                ggAAGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTa                                 >  1:3734549/1‑150 (MQ=255)
                                                                                                                  aaGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAat                               <  1:3283047/150‑1 (MQ=255)
                                                                                                                   aGGGCCTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTAATTAACGGTGAGACAATCAGTATTTGTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatg                              >  2:3335571/1‑150 (MQ=255)
                                                                                                                       ccTGCTGACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGt                          <  2:4100026/150‑1 (MQ=255)
                                                                                                                        ctgctgACGGATGCTGTCTGAGATGACAGGGACGAGGATATCTGCCGCTTTACTTAACGGTGAGCCCATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGACTGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTc                         >  1:2174458/1‑150 (MQ=255)
                                                                                                                        ctgctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTc                         >  2:1802709/1‑150 (MQ=255)
                                                                                                                          gctgACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtt                       <  2:1291303/150‑1 (MQ=255)
                                                                                                                             gACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTg                    <  2:3235827/150‑1 (MQ=255)
                                                                                                                             gACGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTg                    >  2:1876307/1‑150 (MQ=255)
                                                                                                                              aCGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGc                   >  2:613306/1‑150 (MQ=255)
                                                                                                                               cGGATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGcc                  <  1:2085587/150‑1 (MQ=255)
                                                                                                                                ggATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCa                 >  2:2222911/1‑150 (MQ=255)
                                                                                                                                 gATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAg                <  2:1045504/150‑1 (MQ=255)
                                                                                                                                 gATGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAg                <  2:2215249/150‑1 (MQ=255)
                                                                                                                                   tGCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTa              <  2:2069912/150‑1 (MQ=255)
                                                                                                                                    gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAg             >  1:394786/1‑150 (MQ=255)
                                                                                                                                    gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAg             >  2:1128409/1‑150 (MQ=255)
                                                                                                                                    gCTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAg             >  1:2345481/1‑150 (MQ=255)
                                                                                                                                     cTGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGa            >  2:4483755/1‑150 (MQ=255)
                                                                                                                                      tGTCTGAGATGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGAt           <  2:1340612/150‑1 (MQ=255)
                                                                                                                                            agaTGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccgc     >  2:3589098/1‑150 (MQ=255)
                                                                                                                                              aTGACAGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCgcgc   >  1:3974544/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCA  >  CP009273/1661616‑1661908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑