Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 132,639 T→A 11.2% T105S (ACG→TCG)  speE ← spermidine synthase (putrescine aminopropyltransferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273132,6390TA11.2% 732.9 / 64.8 249T105S (ACG→TCG) speEspermidine synthase (putrescine aminopropyltransferase)
Reads supporting (aligned to +/- strand):  ref base T (108/108);  new base A (19/10);  total (131/118)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

ACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCGGTGG  >  CP009273/132491‑132788
                                                                                                                                                    |                                                                                                                                                     
 taTCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTACACCATCgaga                                                                                                                                                     >  1:2791503/2‑150 (MQ=255)
   tCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAtt                                                                                                                                                   <  1:3579706/150‑1 (MQ=255)
   tCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAtt                                                                                                                                                   <  1:26588/150‑1 (MQ=255)
   tCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGAtt                                                                                                                                                   <  2:3283862/150‑1 (MQ=255)
     aaaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATTGTTTGGTAGATACTGACGGCAGAAAGATACGACACCCGCATCGATTTACACCATAGTGATTGa                                                                                                                                                 >  1:1463890/1‑150 (MQ=255)
     aaaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGa                                                                                                                                                 >  2:2026004/1‑150 (MQ=255)
      aaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGAc                                                                                                                                                >  2:749647/1‑150 (MQ=255)
      aaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGAc                                                                                                                                                >  2:2430346/1‑150 (MQ=255)
      aaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGAc                                                                                                                                                <  1:4646494/150‑1 (MQ=255)
        ggTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATTGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATAGATTTCCACCATAGAGATTGACTc                                                                                                                                              >  1:2259512/1‑150 (MQ=255)
         gTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCa                                                                                                                                             >  1:1949410/1‑150 (MQ=255)
             ggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATTGTTGGGTAGATACTGACGGCAGAACCATAAGACACCCCCATCGCTTTACACCAACGTGATTTACTCAACGt                                                                                                                                         >  1:893258/1‑150 (MQ=255)
               ctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGttt                                                                                                                                       >  2:1993303/1‑150 (MQ=255)
                 ggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACAGACGGCAGAACCATACGACACCCGCAGCGATTTACACCATCGTGATTTTCTCaaactgttt                                                                                                                                     >  1:1211430/1‑143 (MQ=255)
                  gTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATTGTTTGGTAGATAATGACGGCACAACGATACGACACCCCCATCGATTTACAACATCGAGATTGACACCACGTTTTTa                                                                                                                                    >  1:3891534/1‑150 (MQ=255)
                  gTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTa                                                                                                                                    <  2:4671645/150‑1 (MQ=255)
                   tttGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTAt                                                                                                                                   <  1:4516643/150‑1 (MQ=255)
                   tttGATTAACGAAATAGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATAAAGACGGCAGAACGATACGACACCCCCATAGCTTTACACCAACCTGAATGACACAACCTTTTTAt                                                                                                                                   >  2:3364938/1‑150 (MQ=255)
                      gATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTc                                                                                                                                <  2:1871337/150‑1 (MQ=255)
                      gATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTc                                                                                                                                <  1:1688042/150‑1 (MQ=255)
                       aTTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCg                                                                                                                               >  1:153963/1‑150 (MQ=255)
                       aTTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTGATTGTTGGGTAGATACAGACGGCAGAACGATAAGAAACCCGCATCGATTTCCACCATCGGGATTTACTCAACGTTTTTATGTCg                                                                                                                               >  1:153275/1‑150 (MQ=255)
                         tAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCAATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCggg                                                                                                                             <  2:1840393/150‑1 (MQ=255)
                               aaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCCACGGTTTTATGTCGGGTTACtt                                                                                                                       >  2:3357420/1‑150 (MQ=255)
                                aTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTc                                                                                                                      <  2:4776240/150‑1 (MQ=255)
                                        cATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACgcagca                                                                                                              <  2:3908656/150‑1 (MQ=255)
                                          tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATg                                                                                                            <  2:1285882/150‑1 (MQ=255)
                                           cgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATgg                                                                                                           <  2:4408869/150‑1 (MQ=255)
                                               gATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCAcc                                                                                                       <  2:2822874/150‑1 (MQ=255)
                                               gATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCAcc                                                                                                       >  2:2250742/1‑150 (MQ=255)
                                               gATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCAcc                                                                                                       <  1:2032580/150‑1 (MQ=255)
                                                aTCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCg                                                                                                      <  2:3488376/150‑1 (MQ=255)
                                                 tCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGt                                                                                                     >  1:2694758/1‑150 (MQ=255)
                                                  cACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTc                                                                                                    <  1:1993303/150‑1 (MQ=255)
                                                  cACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGGTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTc                                                                                                    >  2:4072652/1‑150 (MQ=255)
                                                     cAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTTACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgcc                                                                                                 >  1:1560236/1‑150 (MQ=255)
                                                           aaaGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGTCTCAACGTTTTTATGTCGGGTTACTTAACGCAGCATGGCACCGTCgccgccgcc                                                                                           >  1:1928366/1‑150 (MQ=255)
                                                                 cgGATCGTCGTAGCTACCGGCGTTTTGGTTGGGTAGATACTTAAGGCAGAAAGAAACGACAACCGCAACGAGTTACACCAACGTGATTTACTCAACGTTTTTATGTAGGGTTACATCAAGAAGCATGGCCCCGTCGCCCCCGCCCATAAt                                                                                     >  1:2851582/1‑150 (MQ=255)
                                                                  ggATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATc                                                                                    >  1:2363030/1‑150 (MQ=255)
                                                                   gATCGTCGTAGCTACCGGCGTTATTGTTGGGTAGATACAGACGGCAGAACCATACGACACCCGCATCGATTTACACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCCATAATCa                                                                                   >  1:2193360/1‑150 (MQ=255)
                                                                   gATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCa                                                                                   <  1:780337/150‑1 (MQ=255)
                                                                   gATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATAATGAAGGCAGAACGATAAGACACCCGCATCGATTTACACCATCCAGATTGTCTCAACGTTTTTATTTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCa                                                                                   >  1:125232/1‑150 (MQ=255)
                                                                    aTCGTCGTAGCTACCGGCGTTATGGGTGGGTATATAATGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTAATTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAg                                                                                  >  1:1059514/1‑150 (MQ=255)
                                                                      cgtcgtAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGca                                                                                >  2:2778228/1‑150 (MQ=255)
                                                                       gtcgtAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGACACCGTCGCCGCCGCCGATAATCAGcac                                                                               <  2:1713848/150‑1 (MQ=255)
                                                                         cgtAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACAt                                                                             >  1:805986/1‑150 (MQ=255)
                                                                         cgtAGCTACCGGCGTGATGGTTGGGTAGATACATACGGCAGAACGATACGACACCCCCAACGATTTCCACCAACGTGATTGACGCAACGTTTTTATGTCGGGGTACGTCACGCACCCTGGCACCCGCGCCGCCGCCCCTAAACAGcacaa                                                                             >  1:207963/1‑149 (MQ=255)
                                                                          gtAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATg                                                                            >  2:4668517/1‑150 (MQ=255)
                                                                           tAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGt                                                                           <  1:1569727/150‑1 (MQ=255)
                                                                           tAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGAAACAACGTTTTTATGTAGGGTTACATCACGCAGCAAGGCACCGTCGCCGCCGCCGATAATCAGCACATGt                                                                           >  2:699031/1‑150 (MQ=255)
                                                                              cTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATAAGACACCCGCATCGATTTACACCATCGTGATTTACTCAACGTTTTTATTTCGGGTTACTTCACGCCGCATGGCACCCTCGCCGCCGCCGAAAATAAGCACAAGTTTc                                                                        >  1:1399440/1‑150 (MQ=255)
                                                                               tACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAAAGATACCAAACCCGCATCGATTTACAACATAGTGATTGACTCAACGTTTTTATGTAGGGTTACTTCACGCAGCATGGCACCCTCGCCGCCGCCGATAATCATCACATGTTTcg                                                                       >  2:2853679/1‑150 (MQ=255)
                                                                                aCCGGCGTTATGGTTTGGTAGATACAGACGGCAGAACGATACGACACCCGCAACGATTTCCACCAACGTGATTGACTCAACGTTTTTATTTCGGGTTACATCACGCAGCATGGCACCGTAGCCGCCGCCGATAATAATCACAAGTTTcgc                                                                      >  1:2760486/1‑150 (MQ=255)
                                                                                 ccGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTcgcg                                                                     <  1:3584602/150‑1 (MQ=255)
                                                                                 ccGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTcgcg                                                                     <  1:3584695/150‑1 (MQ=255)
                                                                                  cGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGt                                                                    >  1:1277613/1‑150 (MQ=255)
                                                                                  cGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGt                                                                    >  1:3004529/1‑150 (MQ=255)
                                                                                   ggCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTg                                                                   >  2:4716631/1‑150 (MQ=255)
                                                                                     cGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGAc                                                                 >  2:2467413/1‑150 (MQ=255)
                                                                                         aTGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATg                                                             <  1:3660477/150‑1 (MQ=255)
                                                                                          tGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATgg                                                            <  2:4153064/150‑1 (MQ=255)
                                                                                          tGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATgg                                                            >  2:4365791/1‑150 (MQ=255)
                                                                                           ggTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATTGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATggg                                                           >  2:3532534/1‑150 (MQ=255)
                                                                                           ggTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATggg                                                           >  2:4462307/1‑150 (MQ=255)
                                                                                            gTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGc                                                          <  2:2227878/150‑1 (MQ=255)
                                                                                              tGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCa                                                        >  2:1093585/1‑150 (MQ=255)
                                                                                                  tAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAg                                                    <  2:153275/150‑1 (MQ=255)
                                                                                                  tAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAg                                                    <  2:153963/150‑1 (MQ=255)
                                                                                                      tACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGa                                                >  1:2456195/1‑150 (MQ=255)
                                                                                                      tACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGa                                                >  1:1743861/1‑150 (MQ=255)
                                                                                                        cTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAAc                                              <  1:3233414/150‑1 (MQ=255)
                                                                                                         tGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACa                                             >  1:46594/1‑150 (MQ=255)
                                                                                                           aCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATg                                           >  1:1027808/1‑150 (MQ=255)
                                                                                                           aCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATg                                           >  1:869725/1‑150 (MQ=255)
                                                                                                            cGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATgg                                          >  2:2637088/1‑150 (MQ=255)
                                                                                                             ggCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATggg                                         <  1:3364938/150‑1 (MQ=255)
                                                                                                               cAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtc                                       >  1:4336123/1‑150 (MQ=255)
                                                                                                               cAGAACGATACGACACCCGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtc                                       >  2:175365/1‑150 (MQ=255)
                                                                                                                         cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt                             <  2:1444850/150‑1 (MQ=255)
                                                                                                                                cGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagat                      >  1:1523973/1‑150 (MQ=255)
                                                                                                                                cGCATCGATTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagat                      >  2:3347023/1‑150 (MQ=255)
                                                                                                                                   aTCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa                   >  1:180486/1‑150 (MQ=255)
                                                                                                                                   aTCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa                   >  1:547860/1‑150 (MQ=255)
                                                                                                                                     cGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACt                 >  2:383941/1‑150 (MQ=255)
                                                                                                                                      gATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtc                <  2:1949414/150‑1 (MQ=255)
                                                                                                                                      gATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtc                <  2:3195113/150‑1 (MQ=255)
                                                                                                                                       aTTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTCGCGGAACATGGGTCATCATCTCATGATAGATAAACtcg               >  1:2575944/1‑150 (MQ=255)
                                                                                                                                       aTTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcg               <  2:3163175/150‑1 (MQ=255)
                                                                                                                                       aTTTCCACCATCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcg               <  2:3811611/150‑1 (MQ=255)
                                                                                                                                        tttCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcgt              >  1:3141686/1‑150 (MQ=255)
                                                                                                                                        tttCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcgt              >  1:3141563/1‑150 (MQ=255)
                                                                                                                                            caccaTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTcgcg          <  2:893258/150‑1 (MQ=255)
                                                                                                                                            caccaTCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTcgcg          >  1:2414567/1‑150 (MQ=255)
                                                                                                                                            caccaTCGAGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTcgcg          <  1:472028/150‑1 (MQ=255)
                                                                                                                                                aTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCg      <  1:4015975/150‑1 (MQ=255)
                                                                                                                                                  cGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggt    >  2:4563429/1‑150 (MQ=255)
                                                                                                                                                  cGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggt    >  2:1141561/1‑150 (MQ=255)
                                                                                                                                                  cGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggt    <  1:749647/150‑1 (MQ=255)
                                                                                                                                                   gTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggtg   >  2:3890850/1‑150 (MQ=255)
                                                                                                                                                   gTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCggtg   >  2:1248480/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCGCTCGGTGG  >  CP009273/132491‑132788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑