Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,711,397 C→T 94.6% R28Q (CGA→CAA)  tyrS ← tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,711,3970CT94.6% 1148.2 / 36.7 333R28Q (CGA→CAA) tyrStyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (8/10);  new base T (119/195);  total (127/206)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

ACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGA  >  CP009273/1711249‑1711545
                                                                                                                                                    |                                                                                                                                                    
   cGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGctc                                                                                                                                                  <  2:4239511/150‑1 (MQ=255)
      cgcgCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGc                                                                                                                                               >  1:143944/1‑150 (MQ=255)
        cgccgcCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTa                                                                                                                                             <  1:4633070/150‑1 (MQ=255)
          ccgccTCCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACCATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAc                                                                                                                                           <  1:93614/150‑1 (MQ=255)
          ccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAc                                                                                                                                           <  2:1394723/150‑1 (MQ=255)
           cgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACg                                                                                                                                          >  1:1376784/1‑150 (MQ=255)
           cgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACg                                                                                                                                          >  2:3255733/1‑150 (MQ=255)
                 ccAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCt                                                                                                                                    <  2:1093561/150‑1 (MQ=255)
                  cAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTc                                                                                                                                   <  2:557741/150‑1 (MQ=255)
                  cAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTc                                                                                                                                   <  2:2771586/150‑1 (MQ=255)
                      gcAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtcc                                                                                                                               <  2:934986/150‑1 (MQ=255)
                      gcAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtcc                                                                                                                               <  1:1749701/150‑1 (MQ=255)
                      gcAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtcc                                                                                                                               <  1:4548099/150‑1 (MQ=255)
                        aaCCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtccgt                                                                                                                             <  1:11837/150‑1 (MQ=255)
                          ccGGCTTGTGGCCCGCCTGCTGGAGGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCa                                                                                                                           <  2:544718/150‑1 (MQ=255)
                             gCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCt                                                                                                                        <  1:753089/150‑1 (MQ=255)
                              cTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTg                                                                                                                       <  1:4648636/150‑1 (MQ=255)
                              cTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTg                                                                                                                       >  2:3555638/1‑150 (MQ=255)
                               ttGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTgg                                                                                                                      <  1:2721675/150‑1 (MQ=255)
                                tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTggg                                                                                                                     <  1:674859/150‑1 (MQ=255)
                                tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTggg                                                                                                                     <  1:3063034/150‑1 (MQ=255)
                                  tgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCt                                                                                                                   >  1:862582/1‑150 (MQ=255)
                                     cccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAcc                                                                                                                <  1:2480848/150‑1 (MQ=255)
                                      ccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCa                                                                                                               <  2:4014014/150‑1 (MQ=255)
                                      ccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCa                                                                                                               <  2:4601569/150‑1 (MQ=255)
                                       cGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAg                                                                                                              >  2:4102985/1‑150 (MQ=255)
                                        gCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGc                                                                                                             >  2:2368776/1‑150 (MQ=255)
                                          ctgctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGccc                                                                                                           <  1:1643652/150‑1 (MQ=255)
                                            gctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGccccc                                                                                                         <  1:615488/150‑1 (MQ=255)
                                            gctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGccccc                                                                                                         <  1:3852935/150‑1 (MQ=255)
                                                gAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctc                                                                                                     <  1:4781882/150‑1 (MQ=255)
                                                  aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCtt                                                                                                   >  1:2364396/1‑150 (MQ=255)
                                                      tttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCaa                                                                                               >  2:1704178/1‑150 (MQ=255)
                                                        tCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAtt                                                                                             <  2:136890/150‑1 (MQ=255)
                                                        tCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAtt                                                                                             <  1:566146/150‑1 (MQ=255)
                                                        tCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAtt                                                                                             <  1:2955662/150‑1 (MQ=255)
                                                         cAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTg                                                                                            >  2:1926755/1‑150 (MQ=255)
                                                           ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTTCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGtt                                                                                          <  1:2038219/150‑1 (MQ=255)
                                                           ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGtt                                                                                          >  1:1084450/1‑150 (MQ=255)
                                                           ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGtt                                                                                          <  2:1961168/150‑1 (MQ=255)
                                                            gCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGttt                                                                                         <  2:3461281/150‑1 (MQ=255)
                                                             cATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTa                                                                                        >  1:2592063/1‑150 (MQ=255)
                                                              aTAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTaa                                                                                       >  1:230756/1‑150 (MQ=255)
                                                              aTAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTaa                                                                                       >  1:4432380/1‑150 (MQ=255)
                                                                aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                     <  1:4289433/150‑1 (MQ=255)
                                                                aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                     <  2:4557871/150‑1 (MQ=255)
                                                                  cAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCaa                                                                                   >  2:365818/1‑150 (MQ=255)
                                                                   aaTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAg                                                                                  >  2:963388/1‑150 (MQ=255)
                                                                   aaTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAg                                                                                  <  1:1207589/150‑1 (MQ=255)
                                                                    aTGGAACAAGATGCCCCAAATGCTAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCTATCGGGCGTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCAGCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTACTCTAGt                                                                                 <  1:3937097/150‑1 (MQ=255)
                                                                    aTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                                 <  1:150425/150‑1 (MQ=255)
                                                                    aTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                                 <  1:360316/150‑1 (MQ=255)
                                                                    aTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                                 >  2:2478556/1‑150 (MQ=255)
                                                                      ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTa                                                                               <  1:3529108/150‑1 (MQ=255)
                                                                       gAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAc                                                                              <  2:3108622/150‑1 (MQ=255)
                                                                        aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                             >  2:2867194/1‑150 (MQ=255)
                                                                         aCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTg                                                                            <  2:862582/150‑1 (MQ=255)
                                                                          cAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCCATTGTTTAATCAAGTTACTGc                                                                           >  2:4678693/1‑150 (MQ=255)
                                                                            aGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCtt                                                                         <  2:2687068/150‑1 (MQ=255)
                                                                               tGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGcc                                                                      <  1:4215035/150‑1 (MQ=255)
                                                                                 ccccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCAt                                                                    >  2:1889822/1‑150 (MQ=255)
                                                                                  cccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATc                                                                   <  1:749725/150‑1 (MQ=255)
                                                                                   ccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCa                                                                  <  2:4785478/150‑1 (MQ=255)
                                                                                   ccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCa                                                                  >  2:349769/1‑150 (MQ=255)
                                                                                    cAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaa                                                                 >  1:986102/1‑150 (MQ=255)
                                                                                     aaaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaa                                                                <  2:809233/150‑1 (MQ=255)
                                                                                      aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaaa                                                               <  2:2996348/150‑1 (MQ=255)
                                                                                       aTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAt                                                              >  2:2912553/1‑150 (MQ=255)
                                                                                          cAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtctc                                                           <  2:2634773/150‑1 (MQ=255)
                                                                                           aaGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTcc                                                          >  1:4181167/1‑150 (MQ=255)
                                                                                            aGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCa                                                         <  2:1481698/150‑1 (MQ=255)
                                                                                              cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATg                                                       >  1:3278879/1‑150 (MQ=255)
                                                                                                  cAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtata                                                   <  2:160027/150‑1 (MQ=255)
                                                                                                  cAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtata                                                   <  1:3765784/150‑1 (MQ=255)
                                                                                                   aGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtatat                                                  <  2:2025611/150‑1 (MQ=255)
                                                                                                    gCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtatata                                                 <  2:1444941/150‑1 (MQ=255)
                                                                                                      ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                               <  2:4709837/150‑1 (MQ=255)
                                                                                                      ggTAGGATCGAAGCCGCAATAGAGAGCGATCGGGCCTTGCGCCAGTTGCTCTTCTATCGCTTCCTCGTCCGTCACCTGTGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGATTGCCATCAATATCTCCATGTATATaaa                                               <  2:2308078/150‑2 (MQ=255)
                                                                                                       gTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                              <  2:3856666/150‑1 (MQ=255)
                                                                                                       gTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                              <  1:3255733/150‑1 (MQ=255)
                                                                                                          ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                           <  2:4138963/150‑1 (MQ=255)
                                                                                                           gATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                          >  1:3363699/1‑150 (MQ=255)
                                                                                                              cGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAc                                       >  2:1147491/1‑150 (MQ=255)
                                                                                                              cGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAc                                       <  2:4296024/150‑1 (MQ=255)
                                                                                                                   ccGAAATAGAGCGCGATCGGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTg                                  <  1:3989583/150‑1 (MQ=255)
                                                                                                                      cAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCg                               >  1:970106/1‑150 (MQ=255)
                                                                                                                         tAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTa                            >  1:1953076/1‑150 (MQ=255)
                                                                                                                         tAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAACTCCATGTATATAACGACTGCACCTTTGCCGGTa                            <  2:2185481/150‑1 (MQ=255)
                                                                                                                              cgcgATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGa                       >  2:1008774/1‑150 (MQ=255)
                                                                                                                              cgcgATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGa                       >  1:2104870/1‑150 (MQ=255)
                                                                                                                                cgATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACt                     >  1:1830984/1‑150 (MQ=255)
                                                                                                                                cgATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACt                     <  2:239454/150‑1 (MQ=255)
                                                                                                                                   tCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtttt                  >  1:3904597/1‑150 (MQ=255)
                                                                                                                                    cGGGCCTTGCTCCATTTGCTCTGCTAACGCTTCCTCTTCCGTAACCTGGTCTACCATCCCCCGCTCTTGCAATTGTTTTATCTAGTTACTGATTGCCAACAAAATCTCCATGTTTATAACGACTGCACCTTTGCCGGTACACGACTTTTc                 <  2:608088/150‑1 (MQ=255)
                                                                                                                                      ggCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGc               >  1:900729/1‑150 (MQ=255)
                                                                                                                                          ttGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGa           >  2:1799341/1‑150 (MQ=255)
                                                                                                                                           tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt          <  2:220900/150‑1 (MQ=255)
                                                                                                                                            gcgcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATg         <  2:4359946/150‑1 (MQ=255)
                                                                                                                                               ccAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTTTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa      <  1:2791440/150‑1 (MQ=255)
                                                                                                                                                   tCGCTCTGCTAACGCTTCCCCGTCCGTCACCTGGGCTACCCGCCCCCGCTCTTGCATTTTTTTAATCATTTTACTGGTTGCCATCAAAATCTCCATTTATAAAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAaga  <  1:2856132/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGA  >  CP009273/1711249‑1711545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑