Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,823,835 A→C 7.6% W53G (TGG→GGG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,823,8350AC 7.6% 836.3 / 2.1 342W53G (TGG→GGG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (193/108);  new base C (4/35);  total (197/145)
Fisher's exact test for biased strand distribution p-value = 6.72e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

GATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATA  >  CP009273/1823686‑1823984
                                                                                                                                                      |                                                                                                                                                     
gATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCTTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCa                                                                                                                                                       <  1:232000/150‑1 (MQ=255)
 atcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAg                                                                                                                                                      <  1:3634085/150‑1 (MQ=255)
  tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAgc                                                                                                                                                     >  2:3464471/1‑150 (MQ=255)
  tcatcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAgc                                                                                                                                                     >  2:155182/1‑150 (MQ=255)
    atcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAgcgc                                                                                                                                                   >  2:2986259/1‑150 (MQ=255)
     tcGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGcgcg                                                                                                                                                  >  2:1352340/1‑150 (MQ=255)
      cGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCgg                                                                                                                                                 >  1:1507212/1‑150 (MQ=255)
      cGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCgg                                                                                                                                                 >  2:4425070/1‑150 (MQ=255)
      cGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCgg                                                                                                                                                 >  2:1440543/1‑150 (MQ=255)
          gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                             >  1:4473793/1‑150 (MQ=255)
          gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                             >  2:610830/1‑150 (MQ=255)
          gTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                             <  1:2503260/150‑1 (MQ=255)
          gTCACTTCGGTTGCCGCGTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAAc                                                                                                                                             <  2:4004029/150‑1 (MQ=255)
                tCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCa                                                                                                                                       >  1:4042910/1‑150 (MQ=255)
                    ttGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACggg                                                                                                                                   >  2:3743414/1‑150 (MQ=255)
                        cGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGc                                                                                                                               >  2:4528727/1‑150 (MQ=255)
                        cGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGc                                                                                                                               >  1:4752560/1‑150 (MQ=255)
                          cTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCTGTGCGGCAAAGCGTTCGACAACGGCTTGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGccc                                                                                                                             <  2:1450491/150‑1 (MQ=255)
                            tCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGa                                                                                                                           >  2:2013107/1‑150 (MQ=255)
                               ccgcgcGGCTTACCCGTTTCTCTGGCAATAAGCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAACGCGTTCGACAACGGAATGACTTTCA‑GAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACaa                                                                                                                        <  2:3921490/148‑1 (MQ=255)
                               cAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCAGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACaa                                                                                                                        >  2:1111761/1‑150 (MQ=255)
                                    cgGCTTACCGGTTTCTCTGGCAATAATCGCGGTTATTTCGGCTTTATTGATTTCCAGCTGTGGGGCAAAGCGTTCGACACCGGAATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGGGTAAACGCCGCACGGGCTGCCCGACAAGCCTg                                                                                                                   <  2:1680904/150‑1 (MQ=255)
                                    cgGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTg                                                                                                                   >  1:273633/1‑150 (MQ=255)
                                     ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                  >  1:593981/1‑150 (MQ=255)
                                     ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                                                  >  2:4644711/1‑150 (MQ=255)
                                      gCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCt                                                                                                                 >  1:1997171/1‑150 (MQ=255)
                                          aCCCGTTTCTCTGGCAATAATCGCGGTTATTTCGGCTTTTTTGCTTTCCTGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAc                                                                                                             <  2:2338463/150‑1 (MQ=255)
                                            ccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCTGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GAAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                           <  2:720149/150‑1 (MQ=255)
                                            ccGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAATGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCt                                                                                                           <  2:1182034/150‑1 (MQ=255)
                                              gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                                                                         >  1:1044715/1‑150 (MQ=255)
                                              gTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGa                                                                                                         >  1:4526533/1‑150 (MQ=255)
                                                 tctcTGCCAATAATCGCGTTTAATTCGGATTTATTGCTTTCCAGCAGTGCGGAAAAGCTTTCGACAACGGAATGACGTTCA‑GAAAATCAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                      <  2:2554153/150‑1 (MQ=255)
                                                 tctcGGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGAAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                      <  2:1477523/150‑1 (MQ=255)
                                                 gctctGTCAATCATCGCGGTTAAGTCGGCTTTTTTGCTTGCCCGCAGGGCGCCAAAGCGTTCGCCAACGGCATGACGTTCA‑GCAAATGAAACCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcg                                                                                                      <  2:3041717/149‑1 (MQ=255)
                                                      ggCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatc                                                                                                 >  1:1418202/1‑150 (MQ=255)
                                                        cAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcgg                                                                                               >  2:3449815/1‑150 (MQ=255)
                                                         aataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                                              >  2:820030/1‑150 (MQ=255)
                                                         aataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                                              >  2:3999766/1‑150 (MQ=255)
                                                           taatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                            >  1:2727057/1‑150 (MQ=255)
                                                           taatCGCGGTTAATTCGGATTTATTGTTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGAATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                            <  2:904208/150‑1 (MQ=255)
                                                           taagCGCGTTTAATTCGGATTTATTGCTTACCAGCTGGGCGGAAACGCGTTCGACAACGGCATGACGTTCA‑GAAAATGAAAGCCGCGCCCCGCGCGTACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggcat                                                                                            <  2:904355/150‑1 (MQ=255)
                                                              tCGCGGTTAATTCGGCTTTATTGCTTTCCAGCCGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGcatcat                                                                                         <  2:2270122/150‑1 (MQ=255)
                                                               cgcgGTTATTTCGGCTTTTTTGCTTTCCAGCAGTGCGGAAACGCGTTCGACACCGGCTTGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAtt                                                                                        <  2:2873725/150‑1 (MQ=255)
                                                                gggtttATTTGGGCTTTTTTGTTTCCCAGCTGGGGGGAAATGCGTTCCACACCGGATTGA‑GTTAACGAAAATGAAAGCCGCGCCCCGGGGGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttg                                                                                       <  1:1008114/146‑1 (MQ=255)
                                                                  ggTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcc                                                                                     >  2:3624357/1‑150 (MQ=255)
                                                                   tttATTTCGGTTTTTTTGATTTCCCGCTGGGCGCAAATGGTTTCTACAAGGGATTTAGTTTCA‑CAAAATAAACGCCGCGCCCCGCGCGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                                                                    <  1:2986369/149‑1 (MQ=255)
                                                                     tAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                                                                  <  2:4232994/150‑1 (MQ=255)
                                                                       aTTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                                                >  2:1269974/1‑150 (MQ=255)
                                                                          cGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATa                                                                             >  1:335852/1‑150 (MQ=255)
                                                                           ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                                                            >  2:3399719/1‑150 (MQ=255)
                                                                           ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATaa                                                                            >  2:4308845/1‑150 (MQ=255)
                                                                            gCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAac                                                                           >  1:2956714/1‑150 (MQ=255)
                                                                                tATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCt                                                                       >  2:3826818/1‑150 (MQ=255)
                                                                                   tGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGc                                                                    >  1:755456/1‑150 (MQ=255)
                                                                                      tttCCCGCTGGGCGTCAATGGGTGCGACACGGGCATGACGTACA‑GCAAATCAACGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                                                 <  1:4175710/150‑1 (MQ=255)
                                                                                      tttCCAGCTGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                                                 <  2:661220/150‑1 (MQ=255)
                                                                                        tgcagctgTGCGGCAAAGCGTTCGACAACGGCATTACGTTCA‑GCAATTGAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                                                               <  2:4349668/148‑1 (MQ=255)
                                                                                         cccgatgTGGGGCAATGCTTTCTACATGGTCATGAGGTTAA‑GCAAATGAACGCCGCGCCCAGCGGGCACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATa                                                              <  1:2599109/144‑1 (MQ=255)
                                                                                          cagcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAc                                                             >  1:564684/1‑150 (MQ=255)
                                                                                           agcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATAcc                                                            >  2:2802285/1‑150 (MQ=255)
                                                                                               gTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt                                                        >  1:1329412/1‑150 (MQ=255)
                                                                                               gTGCGGAAATGCGTACGACAACGGCATTACGTTCA‑GAAAATGAAAGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAt                                                        <  1:1871719/150‑1 (MQ=255)
                                                                                                tGCGGCAAAGGGTTCGACAACGGCATGACGTTCA‑GCAAATTAAAGCCGCGCCCCGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAtt                                                       <  1:2160895/150‑1 (MQ=255)
                                                                                                gggggCAAGGGGTTCGCCAACGGCATGACGTTCA‑GCAATTGCACGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGAtt                                                       <  1:4562651/147‑1 (MQ=255)
                                                                                                   ggCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACg                                                    >  1:3262164/1‑150 (MQ=255)
                                                                                                     aaaaGCGTTCGAAAACGGAATGACTTTCA‑GAAAATGAAAGCCGCGCCCCGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCt                                                  <  1:3907904/149‑1 (MQ=255)
                                                                                                      aaaGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCtt                                                 >  2:1912501/1‑150 (MQ=255)
                                                                                                       aaGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTc                                                <  2:1960945/150‑1 (MQ=255)
                                                                                                       aaGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTc                                                >  2:1685412/1‑150 (MQ=255)
                                                                                                          cGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTcaca                                             >  2:2173595/1‑150 (MQ=255)
                                                                                                               gACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa                                        >  2:3688621/1‑150 (MQ=255)
                                                                                                               gACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGa                                        >  2:2465434/1‑150 (MQ=255)
                                                                                                                 cAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATg                                      >  2:124430/1‑150 (MQ=255)
                                                                                                                    cGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATgcgc                                   >  2:3118767/1‑150 (MQ=255)
                                                                                                                     ggCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGcc                                  >  2:1632020/1‑150 (MQ=255)
                                                                                                                       cATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCt                                >  1:1682700/1‑150 (MQ=255)
                                                                                                                           aCGTTCA‑GAAAATTAATGCCGCGCCCAGCGCGTAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGcc                            <  1:1111761/150‑1 (MQ=255)
                                                                                                                            cGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGccc                           >  1:3447796/1‑150 (MQ=255)
                                                                                                                              ttacatcAATTGAACGCCGCGCCCCGCGGGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCg                          <  1:2070404/144‑1 (MQ=255)
                                                                                                                               tCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGtt                        >  2:2395429/1‑150 (MQ=255)
                                                                                                                               tCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGtt                        <  2:1854519/150‑1 (MQ=255)
                                                                                                                                taagaaaaTGAAAGCCGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGtt                        <  1:970017/145‑1 (MQ=255)
                                                                                                                                cA‑GAAATTGAACGCCGCGCCCCGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTa                       <  1:2886601/150‑1 (MQ=255)
                                                                                                                                   gCAAATGAAAGCCGCGCACAGCGCGGAAACGCAGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATAACGCTTCACACGCGATGCGCCATGGCCCGTTATc                     >  2:4746810/1‑150 (MQ=255)
                                                                                                                                     aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                   <  1:3565001/150‑1 (MQ=255)
                                                                                                                                     aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                   >  1:3942897/1‑150 (MQ=255)
                                                                                                                                     aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                   <  1:2938452/150‑1 (MQ=255)
                                                                                                                                     aaaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCa                   >  1:3381557/1‑150 (MQ=255)
                                                                                                                                      aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                  >  1:773911/1‑150 (MQ=255)
                                                                                                                                      aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                  >  2:1395799/1‑150 (MQ=255)
                                                                                                                                      aaTGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAg                  >  2:1325363/1‑150 (MQ=255)
                                                                                                                                        tGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTc                <  2:1313017/150‑1 (MQ=255)
                                                                                                                                        tGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTc                <  2:3178166/150‑1 (MQ=255)
                                                                                                                                          aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAc              <  1:2449991/150‑1 (MQ=255)
                                                                                                                                           aaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCAcc             >  2:1074336/1‑150 (MQ=255)
                                                                                                                                            aGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCg            <  2:2656360/150‑1 (MQ=255)
                                                                                                                                              ccGCGCCCTGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGtt          <  2:3269235/150‑1 (MQ=255)
                                                                                                                                              ccGCGCCCAGCGCGGACACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGtt          <  1:3044737/150‑1 (MQ=255)
                                                                                                                                                     cAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt   <  2:360054/150‑1 (MQ=255)
                                                                                                                                                     cAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCAt   >  1:4776775/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCA‑GCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATA  >  CP009273/1823686‑1823984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑