Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,944,177 C→A 93.9% L201L (CTG→CTT)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,944,1770CA93.9% 1008.8 / 49.5 312L201L (CTG→CTT) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (8/11);  new base A (156/137);  total (164/148)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 326 total aligned reads displayed.

AGCGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATC  >  CP009273/1944028‑1944323
                                                                                                                                                     |                                                                                                                                                  
aGCGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACatcatc                                                                                                                                                    >  2:3637789/1‑150 (MQ=255)
   gcTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATaagc                                                                                                                                                 >  2:4569510/1‑150 (MQ=255)
           cACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGttt                                                                                                                                         >  1:1712807/1‑150 (MQ=255)
           cACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGttt                                                                                                                                         >  1:2731318/1‑150 (MQ=255)
             cTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGa                                                                                                                                       <  1:300224/150‑1 (MQ=255)
              ttCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaa                                                                                                                                      >  2:1941729/1‑150 (MQ=255)
              ttCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaa                                                                                                                                      >  2:1071340/1‑150 (MQ=255)
                cACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaaca                                                                                                                                    <  1:4599576/150‑1 (MQ=255)
                cACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaaca                                                                                                                                    >  2:3001101/1‑150 (MQ=255)
                cACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaaca                                                                                                                                    <  1:856586/150‑1 (MQ=255)
                  cGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGaacaac                                                                                                                                  >  2:2396469/1‑150 (MQ=255)
                    cACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTg                                                                                                                                >  1:1785913/1‑150 (MQ=255)
                     aCTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGc                                                                                                                               >  1:582339/1‑150 (MQ=255)
                         gcggcgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCgggg                                                                                                                           >  1:369065/1‑150 (MQ=255)
                          cggcgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGa                                                                                                                          <  1:161617/150‑1 (MQ=255)
                          cggcgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGa                                                                                                                          >  1:4039335/1‑150 (MQ=255)
                               cgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCg                                                                                                                     >  1:1342643/1‑150 (MQ=255)
                                 gTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                                                                                                                   <  1:3241756/150‑1 (MQ=255)
                                 gTCATGAAAGAAGGTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                                                                                                                   <  2:4034466/150‑1 (MQ=255)
                                  tCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCa                                                                                                                  <  1:3166632/150‑1 (MQ=255)
                                       aaaGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCg                                                                                                             <  1:3815446/150‑1 (MQ=255)
                                       aaaGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCg                                                                                                             >  1:3863612/1‑150 (MQ=255)
                                         agaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTa                                                                                                           <  2:2877434/150‑1 (MQ=255)
                                          gaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAg                                                                                                          <  1:3867350/150‑1 (MQ=255)
                                          gaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAg                                                                                                          <  2:3560710/150‑1 (MQ=255)
                                          gaagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAg                                                                                                          <  2:3065164/150‑1 (MQ=255)
                                            agTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                        >  2:485633/1‑150 (MQ=255)
                                            agTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                        <  2:1758651/150‑1 (MQ=255)
                                                tCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTa                                                                                                    <  1:1656453/150‑1 (MQ=255)
                                                 ccACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTAc                                                                                                   <  1:2540528/150‑1 (MQ=255)
                                                     aTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCttt                                                                                               <  2:3994143/150‑1 (MQ=255)
                                                      tCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtg                                                                                              >  1:1532562/1‑150 (MQ=255)
                                                       cGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgt                                                                                             <  1:3467356/150‑1 (MQ=255)
                                                        gATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgtg                                                                                            <  1:732635/150‑1 (MQ=255)
                                                         aTCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGc                                                                                           >  2:2209666/1‑150 (MQ=255)
                                                           cTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                         >  2:1688984/1‑150 (MQ=255)
                                                           cTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                         >  1:2321862/1‑150 (MQ=255)
                                                               gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACga                                                                                     <  2:1907449/150‑1 (MQ=255)
                                                                 aaaTTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACgaga                                                                                   >  1:4355319/1‑150 (MQ=255)
                                                                       aGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAc                                                                             <  1:1860863/150‑1 (MQ=255)
                                                                        ggCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAcc                                                                            <  2:1206518/150‑1 (MQ=255)
                                                                         gCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCa                                                                           >  2:2030945/1‑150 (MQ=255)
                                                                            gACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGt                                                                        <  1:3367378/150‑1 (MQ=255)
                                                                            gACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGt                                                                        >  1:2040616/1‑150 (MQ=255)
                                                                             aCGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTa                                                                       <  2:4462236/150‑1 (MQ=255)
                                                                                 tCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCa                                                                   <  1:964234/150‑1 (MQ=255)
                                                                                 tCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCa                                                                   <  2:1444412/150‑1 (MQ=255)
                                                                                  cAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAc                                                                  >  2:34612/1‑150 (MQ=255)
                                                                                   aGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcg                                                                 >  2:2266725/1‑150 (MQ=255)
                                                                                    gCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcgc                                                                >  2:4418710/1‑150 (MQ=255)
                                                                                         cAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGcc                                                           <  1:3747532/150‑1 (MQ=255)
                                                                                           ggTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCtt                                                         <  2:2526154/150‑1 (MQ=255)
                                                                                           ggTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCtt                                                         >  2:2490386/1‑150 (MQ=255)
                                                                                            gTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTc                                                        <  1:2920788/150‑1 (MQ=255)
                                                                                             tCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTcc                                                       <  1:4771569/150‑1 (MQ=255)
                                                                                              cTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCg                                                      >  1:3913023/1‑150 (MQ=255)
                                                                                              cTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCg                                                      <  1:2433908/150‑1 (MQ=255)
                                                                                                tCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGc                                                    <  1:4028334/150‑1 (MQ=255)
                                                                                                 cGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCg                                                   >  2:4203095/1‑150 (MQ=255)
                                                                                                     aCGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGc                                               <  2:676992/150‑1 (MQ=255)
                                                                                                      cGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCt                                              >  2:1060857/1‑150 (MQ=255)
                                                                                                       ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCtt                                             >  2:2902267/1‑150 (MQ=255)
                                                                                                         aaGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTg                                           <  1:267526/150‑1 (MQ=255)
                                                                                                             aTTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGATTAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCa                                       >  1:1443466/1‑150 (MQ=255)
                                                                                                              tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAg                                      <  2:1344544/150‑1 (MQ=255)
                                                                                                              tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAg                                      >  1:4551723/1‑150 (MQ=255)
                                                                                                              tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAg                                      >  1:4295810/1‑150 (MQ=255)
                                                                                                                    gATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                                >  1:4466059/1‑150 (MQ=255)
                                                                                                                     aTCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGa                               >  1:3855200/1‑150 (MQ=255)
                                                                                                                      tCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAg                              >  2:3148850/1‑150 (MQ=255)
                                                                                                                      tCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAg                              <  1:1439630/150‑1 (MQ=255)
                                                                                                                        tGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGtt                            >  2:1703374/1‑150 (MQ=255)
                                                                                                                         gATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGttt                           >  1:992358/1‑150 (MQ=255)
                                                                                                                          aTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                          <  2:1785913/150‑1 (MQ=255)
                                                                                                                             gTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGAt                       >  2:3889785/1‑150 (MQ=255)
                                                                                                                             gTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGAt                       >  1:2632238/1‑150 (MQ=255)
                                                                                                                             gTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGAt                       <  2:2391802/150‑1 (MQ=255)
                                                                                                                               aaCGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGt                     >  1:4676256/1‑150 (MQ=255)
                                                                                                                                  ggTCAAAACCGGACCTCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa                  >  2:2101182/1‑150 (MQ=255)
                                                                                                                                  ggTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa                  >  1:2334952/1‑150 (MQ=255)
                                                                                                                                  ggTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa                  >  1:2747623/1‑150 (MQ=255)
                                                                                                                                   gTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAg                 >  1:2230925/1‑150 (MQ=255)
                                                                                                                                      aaaaCCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGaa              <  1:3637789/150‑1 (MQ=255)
                                                                                                                                      aaaaCCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGaa              <  1:1225572/150‑1 (MQ=255)
                                                                                                                                      aaaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGaa              >  2:800105/1‑150 (MQ=255)
                                                                                                                                      aaaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGaa              >  1:4081226/1‑150 (MQ=255)
                                                                                                                                        aaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc            >  1:2871022/1‑150 (MQ=255)
                                                                                                                                         aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGcc           <  1:355398/150‑1 (MQ=255)
                                                                                                                                         aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGcc           <  1:1942713/150‑1 (MQ=255)
                                                                                                                                         aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGcc           <  1:1110285/150‑1 (MQ=255)
                                                                                                                                         aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGcc           <  1:1026832/150‑1 (MQ=255)
                                                                                                                                          ccGGACATCATAATCAGCTGTTTGAACAACTGCGGGGATTGCGTCTGTGCGTAGATTTTTCGTTTGTGCACACGATAAGTCAACTGGTAGTCACGCGCGCCTTCCGGAGTGGCTTTGTTCAGCATCGGTTTTTCGATGTCGAggttgcct          <  2:1642530/150‑7 (MQ=255)
                                                                                                                                          ccGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCg          >  2:3241630/1‑150 (MQ=255)
                                                                                                                                          ccGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCg          <  2:1426417/150‑1 (MQ=255)
                                                                                                                                           cGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTAAACTTTGTGCACACGATAAGGCACCAGGTAGTAACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGAAGTCGAGGAAGCCGt         >  1:1151875/1‑150 (MQ=255)
                                                                                                                                              aCATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGt      >  2:4012772/1‑150 (MQ=255)
                                                                                                                                              aCATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGt      >  1:832968/1‑150 (MQ=255)
                                                                                                                                               catcatAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTc     <  2:1853719/150‑1 (MQ=255)
                                                                                                                                                  cataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATc  <  2:2781810/150‑1 (MQ=255)
                                                                                                                                                  cataaGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATc  <  2:4641549/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGCGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATC  >  CP009273/1944028‑1944323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑