Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,985,366 G→T 32.1% noncoding (17/87 nt) leuZ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,985,3660GT32.1% 632.7 / inf 351noncoding (17/87 nt)leuZtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (136/102);  new base T (71/42);  total (207/144)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

GTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCT  >  CP009273/1985218‑1985515
                                                                                                                                                    |                                                                                                                                                     
  tGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTAtt                                                                                                                                                    <  2:210367/150‑1 (MQ=255)
    cAGGCACAAAAAAACCACCCGATGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTcc                                                                                                                                                  <  1:3776638/150‑1 (MQ=255)
        cacaAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTGATTGCTTTGATTTTATTCTTATCGTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTccacca                                                                                                                                              <  2:2586564/150‑1 (MQ=255)
         aaaaaaaaaCCACCCGACGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCAt                                                                                                                                             <  1:179554/148‑1 (MQ=255)
             aaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCgg                                                                                                                                         >  2:1011627/1‑150 (MQ=255)
             aaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCgg                                                                                                                                         >  1:2869781/1‑150 (MQ=255)
              aaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCggg                                                                                                                                        >  1:1215399/1‑150 (MQ=255)
               aaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGc                                                                                                                                       >  1:2993410/1‑150 (MQ=255)
                aaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCt                                                                                                                                      >  1:3233324/1‑150 (MQ=255)
                 accaccCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTc                                                                                                                                     <  1:3431721/150‑1 (MQ=255)
                     cccGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGga                                                                                                                                 >  1:4088465/1‑150 (MQ=255)
                        gAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGgaaga                                                                                                                              >  2:2367930/1‑150 (MQ=255)
                        gAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaaga                                                                                                                              >  2:423332/1‑150 (MQ=255)
                         aaGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGgaagaa                                                                                                                             >  2:2144711/1‑150 (MQ=255)
                            gtggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGt                                                                                                                          >  1:3911133/1‑150 (MQ=255)
                             tggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTg                                                                                                                         <  1:1576227/150‑1 (MQ=255)
                              ggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTgg                                                                                                                        >  1:689916/1‑150 (MQ=255)
                                  tCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgc                                                                                                                    >  1:196021/1‑150 (MQ=255)
                                  tCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgc                                                                                                                    <  1:2543942/150‑1 (MQ=255)
                                      gACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGt                                                                                                                >  2:537285/1‑150 (MQ=255)
                                      gACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTAGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGt                                                                                                                >  2:599392/1‑150 (MQ=255)
                                       acacTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGtt                                                                                                               <  2:1587637/150‑1 (MQ=255)
                                         acTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTcc                                                                                                             >  1:3339597/1‑150 (MQ=255)
                                          cTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCg                                                                                                            >  2:300422/1‑150 (MQ=255)
                                            gCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                                                          <  2:284861/150‑1 (MQ=255)
                                             cTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATACGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAg                                                                                                         >  1:2447721/1‑150 (MQ=255)
                                                aTTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCg                                                                                                      <  1:2960495/150‑1 (MQ=255)
                                                  tGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGaa                                                                                                    >  1:2671158/1‑150 (MQ=255)
                                                   gCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAc                                                                                                   <  1:4493188/150‑1 (MQ=255)
                                                    cTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                                                  >  1:3848168/1‑150 (MQ=255)
                                                     tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCg                                                                                                 <  2:1781961/150‑1 (MQ=255)
                                                     tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCg                                                                                                 >  1:726073/1‑150 (MQ=255)
                                                      ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg                                                                                                >  2:3020004/1‑150 (MQ=255)
                                                      ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg                                                                                                >  1:1394901/1‑150 (MQ=255)
                                                       tGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGa                                                                                               <  2:3788368/150‑1 (MQ=255)
                                                       tGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGa                                                                                               <  1:2074051/150‑1 (MQ=255)
                                                         aTTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACt                                                                                             >  2:3434458/1‑150 (MQ=255)
                                                            ttATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGa                                                                                          >  1:1196079/1‑150 (MQ=255)
                                                              aTTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACg                                                                                        <  1:1271098/150‑1 (MQ=255)
                                                               ttCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACgg                                                                                       <  2:743421/150‑1 (MQ=255)
                                                               ttCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACgg                                                                                       <  1:1343404/150‑1 (MQ=255)
                                                               ttCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACgg                                                                                       >  1:149753/1‑150 (MQ=255)
                                                                 cTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAt                                                                                     <  2:164144/150‑1 (MQ=255)
                                                                  ttATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGACGAAAGTGGAGGCGCGTTCCGGCGTCGAACCGGACTAGACGGAtt                                                                                    >  2:3354937/1‑150 (MQ=255)
                                                                   tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAttt                                                                                   >  2:4021122/1‑150 (MQ=255)
                                                                      cTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa                                                                                <  2:3828028/150‑1 (MQ=255)
                                                                           ccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCg                                                                           >  2:3931658/1‑150 (MQ=255)
                                                                           ccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCg                                                                           >  2:2746727/1‑150 (MQ=255)
                                                                           ccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCg                                                                           >  1:857753/1‑150 (MQ=255)
                                                                             aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCt                                                                         >  1:1082786/1‑150 (MQ=255)
                                                                             aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCt                                                                         >  1:2160634/1‑150 (MQ=255)
                                                                             aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCt                                                                         >  1:2161497/1‑150 (MQ=255)
                                                                             aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCt                                                                         >  1:4517941/1‑150 (MQ=255)
                                                                               ggTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTAc                                                                       <  2:1318449/150‑1 (MQ=255)
                                                                               ggTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTAc                                                                       <  1:4116185/150‑1 (MQ=255)
                                                                                gTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACa                                                                      >  2:1877587/1‑150 (MQ=255)
                                                                                     cGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAAcc                                                                 >  2:3631908/1‑150 (MQ=255)
                                                                                      ggAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCg                                                                >  2:849667/1‑150 (MQ=255)
                                                                                      ggAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCg                                                                <  2:468204/150‑1 (MQ=255)
                                                                                      ggAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCg                                                                >  2:1178555/1‑150 (MQ=255)
                                                                                        aGCGGGCCTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGACGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACCTAACCGcg                                                              >  2:3063547/1‑149 (MQ=255)
                                                                                        aGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCACTCCGCTACATAACCGCt                                                              >  2:1762791/1‑150 (MQ=255)
                                                                                        aGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATCACCGCt                                                              >  2:4167119/1‑150 (MQ=255)
                                                                                        aGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCt                                                              >  2:675319/1‑150 (MQ=255)
                                                                                          cGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCttt                                                            >  1:2902014/1‑150 (MQ=255)
                                                                                            ggACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGt                                                          <  2:3190624/150‑1 (MQ=255)
                                                                                              aCTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTa                                                        <  2:4111994/150‑1 (MQ=255)
                                                                                               cTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTaa                                                       >  1:3182108/1‑150 (MQ=255)
                                                                                                  gAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAAcgc                                                    >  2:1390974/1‑150 (MQ=255)
                                                                                                  gAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAAcgc                                                    >  1:2712692/1‑150 (MQ=255)
                                                                                                          acaGCGCGAACGTCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAtt                                            <  2:227201/150‑1 (MQ=255)
                                                                                                            aGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAttct                                          <  1:1011627/150‑1 (MQ=255)
                                                                                                            aGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAttct                                          >  1:3210593/1‑150 (MQ=255)
                                                                                                            aGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAttct                                          <  1:4221525/150‑1 (MQ=255)
                                                                                                                   aCGCCGAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGcc                                   >  1:4136957/1‑150 (MQ=255)
                                                                                                                    cGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCt                                  >  2:617976/1‑150 (MQ=255)
                                                                                                                    cGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCt                                  <  2:3538321/150‑1 (MQ=255)
                                                                                                                      ccGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCttt                                >  1:1166570/1‑150 (MQ=255)
                                                                                                                      ccGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCttt                                >  2:480238/1‑150 (MQ=255)
                                                                                                                        gAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCa                              <  1:2226858/150‑1 (MQ=255)
                                                                                                                        gAGGGATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGGGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCa                              >  2:2533414/1‑150 (MQ=255)
                                                                                                                         aGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAg                             <  1:428384/150‑1 (MQ=255)
                                                                                                                         aGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAg                             <  1:2101231/150‑1 (MQ=255)
                                                                                                                          gggATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGc                            >  1:3963432/1‑150 (MQ=255)
                                                                                                                           ggATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGcc                           <  1:4612748/150‑1 (MQ=255)
                                                                                                                            gATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCa                          >  2:151012/1‑150 (MQ=255)
                                                                                                                            gATTTTAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCa                          <  2:2671158/150‑1 (MQ=255)
                                                                                                                                 tAAATCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACAt                     >  1:4427750/1‑150 (MQ=255)
                                                                                                                                 tAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACAt                     <  2:4468011/150‑1 (MQ=255)
                                                                                                                                 tAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACAt                     >  1:623463/1‑150 (MQ=255)
                                                                                                                                    aTCCCTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCg                  >  1:2246727/1‑150 (MQ=255)
                                                                                                                                      cccTTGTGTCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCt                <  2:1074616/150‑1 (MQ=255)
                                                                                                                                         ttGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGa             <  2:454726/150‑1 (MQ=255)
                                                                                                                                          tgtgTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGAc            <  1:152113/150‑1 (MQ=255)
                                                                                                                                           gtgtCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACg           >  1:943710/1‑150 (MQ=255)
                                                                                                                                             gtCTACCTATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGcc         <  2:1996859/150‑1 (MQ=255)
                                                                                                                                             gtCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGcc         <  2:3848200/150‑1 (MQ=255)
                                                                                                                                                tACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGAt      <  1:2437678/150‑1 (MQ=255)
                                                                                                                                                  ccGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGt    <  1:537285/150‑1 (MQ=255)
                                                                                                                                                    gATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCt  <  2:758883/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCT  >  CP009273/1985218‑1985515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑