Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,991,843 G→A 30.3% L40F (CTC→TTC)  yecS ← ABC family putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,991,8430GA30.3% 442.4 / 239.8 267L40F (CTC→TTC) yecSABC family putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base G (94/92);  new base A (45/36);  total (139/128)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991694‑1991992
                                                                                                                                                     |                                                                                                                                                     
cGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCgag                                                                                                                                                       >  2:4038455/1‑150 (MQ=255)
   aaTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAAt                                                                                                                                                    <  2:780894/150‑1 (MQ=255)
    aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGACTa                                                                                                                                                   >  1:3476671/1‑150 (MQ=255)
    aTGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATa                                                                                                                                                   <  2:3530581/150‑1 (MQ=255)
        gATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAAcc                                                                                                                                               >  2:263902/1‑150 (MQ=255)
          tCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGTCAGACGCATCAGCGCGAGAATAAACCCg                                                                                                                                             >  2:2950890/1‑150 (MQ=255)
            tAATTCGATCCCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           <  2:1503873/150‑1 (MQ=255)
            tAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAg                                                                                                                                           <  2:3806813/150‑1 (MQ=255)
               ttCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAg                                                                                                                                        >  1:1397317/1‑150 (MQ=255)
                  gATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTaa                                                                                                                                     >  2:2601340/1‑150 (MQ=255)
                  gATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTaa                                                                                                                                     >  2:3587427/1‑150 (MQ=255)
                  gATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTaa                                                                                                                                     >  2:3107794/1‑150 (MQ=255)
                   aTACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAg                                                                                                                                    <  1:78116/150‑1 (MQ=255)
                     aCCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGcc                                                                                                                                  <  2:2763588/150‑1 (MQ=255)
                      ccAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCa                                                                                                                                 <  2:1969023/150‑1 (MQ=255)
                       cAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaa                                                                                                                                <  2:2343990/150‑1 (MQ=255)
                       cAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaa                                                                                                                                <  2:2423545/150‑1 (MQ=255)
                       cAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaa                                                                                                                                >  2:3231834/1‑150 (MQ=255)
                       cAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaa                                                                                                                                <  2:3701251/150‑1 (MQ=255)
                       cAAACTGCGGCAGGCCGTAATAGAGCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCaa                                                                                                                                <  1:2583046/150‑1 (MQ=255)
                            tGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAAc                                                                                                                           <  1:2196863/150‑1 (MQ=255)
                                gCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgc                                                                                                                       <  1:940595/150‑1 (MQ=255)
                                 cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                      >  2:2450305/1‑150 (MQ=255)
                                 cAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATgcc                                                                                                                      >  1:101096/1‑150 (MQ=255)
                                  aGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccg                                                                                                                     >  2:1436514/1‑150 (MQ=255)
                                  aGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccg                                                                                                                     >  1:4777779/1‑150 (MQ=255)
                                   ggCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccgc                                                                                                                    >  1:2863258/1‑150 (MQ=255)
                                   ggCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATgccgc                                                                                                                    <  1:4347821/150‑1 (MQ=255)
                                     ccGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGccgccg                                                                                                                  <  1:4257736/150‑1 (MQ=255)
                                      cGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGa                                                                                                                 >  1:3859816/1‑150 (MQ=255)
                                         aaTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATAc                                                                                                              >  2:3153520/1‑150 (MQ=255)
                                          aTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACt                                                                                                             >  2:300005/1‑150 (MQ=255)
                                          aTAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACt                                                                                                             <  1:4792687/150‑1 (MQ=255)
                                            aGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGa                                                                                                           <  2:1960085/150‑1 (MQ=255)
                                            aGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGCCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGa                                                                                                           >  2:1855420/1‑150 (MQ=255)
                                              aTCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGt                                                                                                         >  1:1120962/1‑150 (MQ=255)
                                               tCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGtt                                                                                                        >  1:1999530/1‑150 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAACACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     <  2:4565158/150‑1 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     >  1:4630833/1‑150 (MQ=255)
                                                  tAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCa                                                                                                     <  2:3180939/150‑1 (MQ=255)
                                                   aaaCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAg                                                                                                    >  2:1499541/1‑150 (MQ=255)
                                                    aaCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGc                                                                                                   >  1:1325283/1‑150 (MQ=255)
                                                     aCAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCg                                                                                                  <  2:148193/150‑1 (MQ=255)
                                                       aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                >  2:599868/1‑150 (MQ=255)
                                                       aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                >  1:1840540/1‑150 (MQ=255)
                                                       aGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGta                                                                                                >  2:1253309/1‑150 (MQ=255)
                                                        gTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGtat                                                                                               <  2:3085731/150‑1 (MQ=255)
                                                        gTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATGGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGtat                                                                                               <  2:33680/150‑1 (MQ=255)
                                                          tgtgCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATAc                                                                                             >  2:3894109/1‑150 (MQ=255)
                                                               gATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGc                                                                                        >  2:1681488/1‑150 (MQ=255)
                                                                  gAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGcccc                                                                                     >  1:2139977/1‑150 (MQ=255)
                                                                     cggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCttt                                                                                  <  1:2491288/150‑1 (MQ=255)
                                                                      ggcgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTc                                                                                 >  1:667428/1‑150 (MQ=255)
                                                                        cgTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcag                                                                               >  1:2717927/1‑150 (MQ=255)
                                                                          tACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcagca                                                                             >  1:147811/1‑150 (MQ=255)
                                                                           aCCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTcagcag                                                                            <  2:1209526/150‑1 (MQ=255)
                                                                              aCGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGaa                                                                         <  2:4470175/150‑1 (MQ=255)
                                                                                gAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACg                                                                       >  1:1963619/1‑150 (MQ=255)
                                                                                gAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACg                                                                       <  1:278899/150‑1 (MQ=255)
                                                                                 aaaaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACgg                                                                      >  2:417983/1‑150 (MQ=255)
                                                                                 aaaaaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACgg                                                                      <  1:4736286/150‑1 (MQ=255)
                                                                                    aaTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCaa                                                                   >  1:2309988/1‑150 (MQ=255)
                                                                                     aTAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAg                                                                  >  2:2904824/1‑150 (MQ=255)
                                                                                       agagATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGa                                                                <  1:1665611/150‑1 (MQ=255)
                                                                                         agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                              <  2:2664341/150‑1 (MQ=255)
                                                                                         agaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAt                                                              <  2:101096/150‑1 (MQ=255)
                                                                                          gaTATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATc                                                             <  2:4731657/150‑1 (MQ=255)
                                                                                              taAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATa                                                         >  1:963831/1‑150 (MQ=255)
                                                                                               aaaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATaa                                                        >  2:3431155/1‑150 (MQ=255)
                                                                                                aaaaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAAc                                                       >  2:805574/1‑150 (MQ=255)
                                                                                                  caCGCGCCAGCCAGCGCTCGGGCCAGATTGGCGACAGACTCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTCCAGCAGGAACGGCAAGGAATCAATAACCa                                                     <  2:1639938/149‑1 (MQ=255)
                                                                                                  aaCGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCa                                                     >  2:3092389/1‑150 (MQ=255)
                                                                                                    cgcgCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGt                                                   >  1:4054814/1‑150 (MQ=255)
                                                                                                     gcgcCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGtt                                                  <  1:2413631/150‑1 (MQ=255)
                                                                                                      cgcCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTg                                                 <  1:3967467/150‑1 (MQ=255)
                                                                                                       gccagccaGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGt                                                <  1:4399846/150‑1 (MQ=255)
                                                                                                          agccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGtata                                             >  2:3101180/1‑150 (MQ=255)
                                                                                                          agccagcGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGtata                                             >  2:2197309/1‑150 (MQ=255)
                                                                                                               gcgcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTc                                        <  2:2529674/150‑1 (MQ=255)
                                                                                                                cgcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCCAGGAATCAATAACCAGTTGTATACTTTCt                                       >  2:1335373/1‑150 (MQ=255)
                                                                                                                 gcACGGGCCAGATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCtt                                      <  1:4744058/150‑1 (MQ=255)
                                                                                                                          aGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCg                             >  1:3584407/1‑150 (MQ=255)
                                                                                                                           gATTGGCGACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGt                            <  1:523485/150‑1 (MQ=255)
                                                                                                                            aTTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGtt                           <  1:269360/150‑1 (MQ=255)
                                                                                                                                 cGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCgg                      <  1:4124372/150‑1 (MQ=255)
                                                                                                                                  gACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGa                     >  2:1731927/1‑150 (MQ=255)
                                                                                                                                  gACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGa                     <  1:512244/150‑1 (MQ=255)
                                                                                                                                  gACAGACGCATCAGCGCGAAAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGa                     >  2:2459769/1‑150 (MQ=255)
                                                                                                                                   aCAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAg                    >  2:4713432/1‑150 (MQ=255)
                                                                                                                                   aCAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAg                    >  2:283404/1‑150 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCt                  >  1:344667/1‑150 (MQ=255)
                                                                                                                                     aGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAAAAACCAGTTGTATACTTTCTAGCATGAGAGTTTCCGGAGcg                  >  1:3233895/1‑149 (MQ=255)
                                                                                                                                      gACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTa                 >  2:1668407/1‑150 (MQ=255)
                                                                                                                                           aTCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGt            >  2:1411266/1‑150 (MQ=255)
                                                                                                                                             cAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTGTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgg          >  2:65814/1‑150 (MQ=255)
                                                                                                                                              aGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTggg         <  2:4213082/150‑1 (MQ=255)
                                                                                                                                                cgcgAAAATAAACCCGAGCAGTACGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGga       <  1:4482181/150‑1 (MQ=255)
                                                                                                                                                 gcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat      <  1:1499541/150‑1 (MQ=255)
                                                                                                                                                   gagaATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgatga    >  2:333651/1‑150 (MQ=255)
                                                                                                                                                     gaATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATa  <  2:963083/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGAATGGGATCTAATTCGATACCAAACTGCGGCAGGCCGTAATAGATCATAAACAGTTGTGCGATGAGCGGCGTACCACGAAAAATAGAGATATAAAAACGCGCCAGCCAGCGCACGGGCCAGATTGGCGACAGACGCATCAGCGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATA  >  CP009273/1991694‑1991992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑