Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,041,982 A→T 12.8% S1188S (TCA→TCT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,041,9820AT12.8% 846.3 / 94.7 273S1188S (TCA→TCT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base A (117/121);  new base T (20/15);  total (137/136)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 296 total aligned reads displayed.

TTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCA  >  CP009273/2041833‑2042131
                                                                                                                                                     |                                                                                                                                                     
 ttGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATtcagctcag                                                                                                                                                      <  1:1007381/150‑1 (MQ=255)
    tgGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCct                                                                                                                                                   <  2:2141887/150‑1 (MQ=255)
      gCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCctct                                                                                                                                                 <  2:3255736/150‑1 (MQ=255)
       cGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCtt                                                                                                                                                <  1:4016694/150‑1 (MQ=255)
        ggACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTc                                                                                                                                               <  1:455408/150‑1 (MQ=255)
            aaaGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGa                                                                                                                                           >  2:3953774/1‑150 (MQ=255)
              aGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACt                                                                                                                                         <  1:2207876/150‑1 (MQ=255)
                 cTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCAc                                                                                                                                      <  2:3110990/150‑1 (MQ=255)
                 cTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCAc                                                                                                                                      <  2:3574792/150‑1 (MQ=255)
                    ggCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCt                                                                                                                                   <  1:3066125/150‑1 (MQ=255)
                     gCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTg                                                                                                                                  <  2:453883/150‑1 (MQ=255)
                      cTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGa                                                                                                                                 >  2:4278395/1‑150 (MQ=255)
                      cTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGa                                                                                                                                 <  1:297653/150‑1 (MQ=255)
                       tCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGAc                                                                                                                                >  1:2038318/1‑150 (MQ=255)
                        cAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGAcc                                                                                                                               >  2:3295225/1‑150 (MQ=255)
                        cAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGAcc                                                                                                                               <  1:4099908/150‑1 (MQ=255)
                           gTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCg                                                                                                                            <  2:1748061/150‑1 (MQ=255)
                           gTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCg                                                                                                                            <  1:386079/150‑1 (MQ=255)
                              gTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGa                                                                                                                         >  1:596457/1‑150 (MQ=255)
                                 cTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagta                                                                                                                      <  1:3980221/150‑1 (MQ=255)
                                  tGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaa                                                                                                                     <  2:1477070/150‑1 (MQ=255)
                                   gCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaag                                                                                                                    <  1:4207513/150‑1 (MQ=255)
                                    cAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACTGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaagt                                                                                                                   >  1:2883812/1‑150 (MQ=255)
                                    cAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGagtcagt                                                                                                                   >  2:3594921/1‑150 (MQ=255)
                                     aGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGagtaagta                                                                                                                  <  1:4410131/150‑1 (MQ=255)
                                      gATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAgtaagtaa                                                                                                                 >  1:3936884/1‑150 (MQ=255)
                                               aGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGa                                                                                                        >  2:2938557/1‑150 (MQ=255)
                                                     gaTCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTgg                                                                                                  >  2:1870915/1‑150 (MQ=255)
                                                         acaGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATc                                                                                              >  2:3187074/1‑150 (MQ=255)
                                                         acaGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATc                                                                                              >  2:2775369/1‑150 (MQ=255)
                                                          caGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATcg                                                                                             <  1:1624753/150‑1 (MQ=255)
                                                          caGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATcg                                                                                             >  2:4769871/1‑150 (MQ=255)
                                                            ggTAAGGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATcgcg                                                                                           <  1:2073370/150‑1 (MQ=255)
                                                             gTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCgcgc                                                                                          >  1:1098182/1‑150 (MQ=255)
                                                                 tGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGc                                                                                      >  2:1985038/1‑150 (MQ=255)
                                                                   gCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGTTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCa                                                                                    <  1:680231/150‑1 (MQ=255)
                                                                    cGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCAc                                                                                   >  1:4641173/1‑150 (MQ=255)
                                                                       cGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCActct                                                                                >  1:1268928/1‑150 (MQ=255)
                                                                         aTAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCg                                                                              <  2:2213583/150‑1 (MQ=255)
                                                                         aTAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCg                                                                              <  2:1097795/150‑1 (MQ=255)
                                                                          tAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGc                                                                             >  2:4449037/1‑150 (MQ=255)
                                                                            gcgcAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAg                                                                           <  2:436284/150‑1 (MQ=255)
                                                                               cAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCg                                                                        <  2:4010968/150‑1 (MQ=255)
                                                                                aaCGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGt                                                                       >  2:2193212/1‑150 (MQ=255)
                                                                                 aCGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGtt                                                                      <  1:1127427/150‑1 (MQ=255)
                                                                                 aCGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGtt                                                                      <  2:2271678/150‑1 (MQ=255)
                                                                                   gCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGc                                                                    >  1:1797161/1‑150 (MQ=255)
                                                                                    cTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGcc                                                                   <  1:973060/150‑1 (MQ=255)
                                                                                       aCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCttt                                                                <  2:2363451/150‑1 (MQ=255)
                                                                                       aCTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCttt                                                                <  1:1325528/150‑1 (MQ=255)
                                                                                          gCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGt                                                             >  1:3925252/1‑150 (MQ=255)
                                                                                           cAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTg                                                            >  2:1039988/1‑150 (MQ=255)
                                                                                            aaCGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTga                                                           >  2:673557/1‑150 (MQ=255)
                                                                                            aaCGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTga                                                           >  2:2564836/1‑150 (MQ=255)
                                                                                             aCGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTgag                                                          <  1:1168767/150‑1 (MQ=255)
                                                                                                   aaaGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACg                                                    >  1:1758658/1‑150 (MQ=255)
                                                                                                      gATCAGTTCGACAATGAGGTGAATACTCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGtt                                                 <  2:1909150/150‑1 (MQ=255)
                                                                                                      gATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGtt                                                 <  1:745094/150‑1 (MQ=255)
                                                                                                          atttCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTg                                             <  1:3389510/148‑1 (MQ=255)
                                                                                                          aGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTg                                             <  1:4456141/150‑1 (MQ=255)
                                                                                                            ttCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCa                                           >  1:475300/1‑150 (MQ=255)
                                                                                                               gACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCa                                        <  1:3267593/150‑1 (MQ=255)
                                                                                                                aCAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCAc                                       >  2:4603049/1‑150 (MQ=255)
                                                                                                                 cAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACt                                      <  1:3786602/150‑1 (MQ=255)
                                                                                                                    tGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGc                                   <  1:3588289/150‑1 (MQ=255)
                                                                                                                    tGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGc                                   <  1:3588359/150‑1 (MQ=255)
                                                                                                                     gAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCt                                  <  1:902307/150‑1 (MQ=255)
                                                                                                                     gAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCt                                  >  1:2469190/1‑150 (MQ=255)
                                                                                                                      aGGTGAATAATCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCta                                 <  2:1402770/150‑1 (MQ=255)
                                                                                                                      aGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCta                                 <  2:4138084/150‑1 (MQ=255)
                                                                                                                       ggTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCtaa                                <  1:2867951/150‑1 (MQ=255)
                                                                                                                         tGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCtaata                              <  2:3503233/150‑1 (MQ=255)
                                                                                                                           aataatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTaataat                            <  2:1278578/150‑1 (MQ=255)
                                                                                                                           aataatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTaataat                            >  2:636360/1‑150 (MQ=255)
                                                                                                                           aataatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACACCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTaataat                            >  2:873401/1‑150 (MQ=255)
                                                                                                                            ataatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATg                           >  1:2706077/1‑150 (MQ=255)
                                                                                                                            agaatCTTACGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTATTAATg                           <  1:1050682/148‑1 (MQ=255)
                                                                                                                             taatCTTCCGTTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATgg                          <  1:3722105/150‑1 (MQ=255)
                                                                                                                              aatCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGt                         <  1:2296558/150‑1 (MQ=255)
                                                                                                                               atCTTCCGGTAACATTCAGCTCTGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCCTCGCGCAGGCCACTCTCGCAGGCGTTGCCTGTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTg                        >  2:3397225/1‑150 (MQ=255)
                                                                                                                                 cTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTGTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGcc                      >  1:4444970/1‑150 (MQ=255)
                                                                                                                                   tCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGTAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAg                    <  2:3128897/150‑1 (MQ=255)
                                                                                                                                   tCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAg                    <  2:742672/150‑1 (MQ=255)
                                                                                                                                    ccGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGc                   >  2:3040555/1‑150 (MQ=255)
                                                                                                                                    ccGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGc                   <  2:1139776/150‑1 (MQ=255)
                                                                                                                                     cGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCg                  >  2:4598696/1‑150 (MQ=255)
                                                                                                                                      ggTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGa                 >  1:1790346/1‑150 (MQ=255)
                                                                                                                                        tAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGTGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGTCTAATAATGGTGCCAGCGaca               <  2:1021043/150‑1 (MQ=255)
                                                                                                                                        tAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGaca               <  1:4278395/150‑1 (MQ=255)
                                                                                                                                          aCATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGacaac             <  2:3742335/150‑1 (MQ=255)
                                                                                                                                           cATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGacaaca            >  1:687744/1‑150 (MQ=255)
                                                                                                                                           cATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGacaaca            <  1:2609869/150‑1 (MQ=255)
                                                                                                                                            aTTCAGCTCTGCCTCTTCGGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGAcaacaa           >  2:3805586/1‑150 (MQ=255)
                                                                                                                                              tcagctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACaaaa         >  2:4305203/1‑150 (MQ=255)
                                                                                                                                               cagctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAAc        >  1:3155374/1‑150 (MQ=255)
                                                                                                                                               cagctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAAc        >  2:2975057/1‑150 (MQ=255)
                                                                                                                                                agctcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACt       <  2:4218408/150‑1 (MQ=255)
                                                                                                                                                   tcagcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACtgtg    <  1:4614727/150‑1 (MQ=255)
                                                                                                                                                     agcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCa  >  2:440364/1‑150 (MQ=255)
                                                                                                                                                     agcCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCa  <  2:2646490/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCAGATATCAAAAGATGAGATCACAGGTAATGGCGTCGATAGCGCAACGCTAACTGCAACGGTTAAAGATCAGTTCGACAATGAGGTGAATAATCTTCCGGTAACATTCAGCTCAGCCTCTTCAGGACTCACCCTGACCCCGGGAGTAAGTAATACCAACGAGTCTGGCATCGCGCAGGCCACTCTCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCA  >  CP009273/2041833‑2042131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑