Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,098,995 C→A 100% G182* (GGA→TGA)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,098,9950CA95.1% 681.7 / 21.2 204G182* (GGA→TGA) wbbId‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base A (83/111);  total (87/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

AGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGAC  >  CP009273/2098848‑2099140
                                                                                                                                                   |                                                                                                                                                 
aGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCAtt                                                                                                                                                 >  1:4715453/1‑150 (MQ=255)
aGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCAtt                                                                                                                                                 <  1:2565044/150‑1 (MQ=255)
  gTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCa                                                                                                                                               >  1:490698/1‑150 (MQ=255)
    ttCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCCGGCCTGGGAGGTTAATCTTGTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGt                                                                                                                                             <  2:4444393/150‑1 (MQ=255)
    ttCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGt                                                                                                                                             <  2:798565/150‑1 (MQ=255)
      cGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtat                                                                                                                                           <  2:1524026/150‑1 (MQ=255)
       gACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAATTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtata                                                                                                                                          >  2:2099423/1‑150 (MQ=255)
       gACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtata                                                                                                                                          <  1:3852644/150‑1 (MQ=255)
       gACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtata                                                                                                                                          <  1:3852527/150‑1 (MQ=255)
         cAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtatata                                                                                                                                        <  1:3810824/150‑1 (MQ=255)
            aaTCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGa                                                                                                                                     >  2:2413248/1‑150 (MQ=255)
                     cccAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAtt                                                                                                                            <  1:407195/150‑1 (MQ=255)
                        aaaTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTAt                                                                                                                         <  2:247822/150‑1 (MQ=255)
                           tGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGcc                                                                                                                      <  1:1240889/150‑1 (MQ=255)
                           tGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGcc                                                                                                                      <  1:1020194/150‑1 (MQ=255)
                            gAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                     <  1:2175587/150‑1 (MQ=255)
                            gAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                     <  1:4620347/150‑1 (MQ=255)
                             aGTCCAAATTGCATGCCTGGGAGGTTAATC‑TTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAg                                                                                                                   <  2:3256211/150‑1 (MQ=255)
                                ccAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaa                                                                                                                 >  2:4676836/1‑150 (MQ=255)
                                ccAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaa                                                                                                                 >  2:699362/1‑150 (MQ=255)
                                ccAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaa                                                                                                                 >  2:1588177/1‑150 (MQ=255)
                                 cAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaaa                                                                                                                >  1:3235666/1‑150 (MQ=255)
                                  aaaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAg                                                                                                               >  1:3117610/1‑150 (MQ=255)
                                  aaaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAg                                                                                                               <  2:2509514/150‑1 (MQ=255)
                                       gCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGc                                                                                                          >  1:4090875/1‑150 (MQ=255)
                                        cATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGcc                                                                                                         <  2:3483124/150‑1 (MQ=255)
                                          tGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAg                                                                                                       >  2:4517631/1‑150 (MQ=255)
                                              tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                   >  2:487460/1‑150 (MQ=255)
                                              tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                   >  2:3210243/1‑150 (MQ=255)
                                              tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                   <  1:3140305/150‑1 (MQ=255)
                                                  aGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATg                                                                                               >  2:3633731/1‑150 (MQ=255)
                                                  aGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATg                                                                                               >  2:2892160/1‑150 (MQ=255)
                                                    gTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAc                                                                                             >  2:425718/1‑150 (MQ=255)
                                                     ttAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAcc                                                                                            >  1:2186711/1‑150 (MQ=255)
                                                      tAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAccc                                                                                           >  1:1356600/1‑150 (MQ=255)
                                                       aaTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAcccc                                                                                          >  2:2059484/1‑150 (MQ=255)
                                                          cTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCg                                                                                       <  1:3094398/150‑1 (MQ=255)
                                                          cTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCg                                                                                       <  1:3548612/150‑1 (MQ=255)
                                                          cTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCg                                                                                       >  2:4233316/1‑150 (MQ=255)
                                                           ttttCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGt                                                                                      >  1:1013005/1‑150 (MQ=255)
                                                            tttCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGtt                                                                                     <  1:1665917/150‑1 (MQ=255)
                                                              tCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGc                                                                                   >  1:684164/1‑150 (MQ=255)
                                                               ccGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCt                                                                                  >  2:2180364/1‑150 (MQ=255)
                                                                cGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCtt                                                                                 <  1:2280289/150‑1 (MQ=255)
                                                                 ggAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTa                                                                                <  1:4190548/150‑1 (MQ=255)
                                                                  gagaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTAt                                                                               <  2:4466923/150‑1 (MQ=255)
                                                                    gaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTACTTATcc                                                                             >  2:1183557/1‑150 (MQ=255)
                                                                     aTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCg                                                                            <  1:3743456/150‑1 (MQ=255)
                                                                          gCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACa                                                                       <  1:733135/150‑1 (MQ=255)
                                                                          gCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACa                                                                       <  2:2646089/150‑1 (MQ=255)
                                                                              cAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtctc                                                                   <  2:2221459/150‑1 (MQ=255)
                                                                               aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCg                                                                  <  1:4261012/150‑1 (MQ=255)
                                                                                aaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGa                                                                 <  2:2294277/150‑1 (MQ=255)
                                                                                   cTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGc                                                              <  2:3439139/150‑1 (MQ=255)
                                                                                      ccAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTcc                                                           >  1:1855509/1‑150 (MQ=255)
                                                                                       cAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCca                                                          <  2:2792212/150‑1 (MQ=255)
                                                                                       cAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCca                                                          <  2:2792062/150‑1 (MQ=255)
                                                                                        aaGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCcac                                                         >  1:4657293/1‑150 (MQ=255)
                                                                                        aaGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCcac                                                         >  1:1770617/1‑150 (MQ=255)
                                                                                          gATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACAt                                                       >  2:679162/1‑150 (MQ=255)
                                                                                          gATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACAt                                                       >  1:2447741/1‑150 (MQ=255)
                                                                                            tatTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCa                                                     >  2:737460/1‑150 (MQ=255)
                                                                                             atTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAg                                                    <  2:4292337/150‑1 (MQ=255)
                                                                                              tttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGa                                                   >  2:3999716/1‑150 (MQ=255)
                                                                                              tttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGa                                                   >  1:1621849/1‑150 (MQ=255)
                                                                                              tttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGa                                                   <  1:3969817/150‑1 (MQ=255)
                                                                                               ttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGAt                                                  <  2:177805/150‑1 (MQ=255)
                                                                                                tAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATg                                                 >  1:1146015/1‑150 (MQ=255)
                                                                                                tAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATg                                                 <  1:2781835/150‑1 (MQ=255)
                                                                                                 aGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATga                                                >  2:1827390/1‑150 (MQ=255)
                                                                                                   gATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgaga                                              <  2:3730791/150‑1 (MQ=255)
                                                                                                    aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                             <  2:826134/150‑1 (MQ=255)
                                                                                                    aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                             <  2:1356600/150‑1 (MQ=255)
                                                                                                     ttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACg                                            <  1:3697570/150‑1 (MQ=255)
                                                                                                        tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                         >  1:1053370/1‑150 (MQ=255)
                                                                                                          tttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTa                                       >  1:2502572/1‑150 (MQ=255)
                                                                                                           ttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTaa                                      >  1:4401163/1‑150 (MQ=255)
                                                                                                            tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTACGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAt                                     <  2:2773368/150‑1 (MQ=255)
                                                                                                                 tCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAat                                >  2:3383400/1‑150 (MQ=255)
                                                                                                                 tCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAat                                >  1:1945795/1‑150 (MQ=255)
                                                                                                                  cATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                               <  1:3106578/150‑1 (MQ=255)
                                                                                                                   aTAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAatat                              <  1:2045938/150‑1 (MQ=255)
                                                                                                                       tttaaacCAAAGAGAGTAAAATCGCATAATTAAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGAATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATAttttt                          <  2:4135681/145‑1 (MQ=255)
                                                                                                                        tGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTa                         <  1:1276505/150‑1 (MQ=255)
                                                                                                                             ccAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCt                    <  2:3456789/150‑1 (MQ=255)
                                                                                                                             ccAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCt                    >  2:1666066/1‑150 (MQ=255)
                                                                                                                               aaaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCttt                  <  1:3343702/150‑1 (MQ=255)
                                                                                                                               aaaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCttt                  >  1:883568/1‑150 (MQ=255)
                                                                                                                                aaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTg                 >  2:836123/1‑150 (MQ=255)
                                                                                                                                aaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTg                 >  2:2958057/1‑150 (MQ=255)
                                                                                                                                  gagagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAt               <  1:2888762/150‑1 (MQ=255)
                                                                                                                                    gagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAttt             >  2:4777051/1‑150 (MQ=255)
                                                                                                                                     agTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtttt            <  2:1001462/150‑1 (MQ=255)
                                                                                                                                     agTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtttt            <  1:3583606/150‑1 (MQ=255)
                                                                                                                                       tAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAt          <  1:487447/150‑1 (MQ=255)
                                                                                                                                        aaaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATc         <  1:1166329/150‑1 (MQ=255)
                                                                                                                                         aaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATcc        >  2:1087243/1‑150 (MQ=255)
                                                                                                                                          aaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCt       <  2:3072890/150‑1 (MQ=255)
                                                                                                                                            tCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTga     <  2:1034787/150‑1 (MQ=255)
                                                                                                                                               catcatTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGAc  <  2:516253/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGAC  >  CP009273/2098848‑2099140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑