Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 30.0% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT30.0% 198.8 / 21.6 129intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (38/44);  new base T (20/22);  total (58/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873
                                                                                                                                                       |                                                                                                                                                        
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATg                                                                                                                                                          <  2:4151998/150‑1 (MQ=255)
 ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATCTTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGa                                                                                                                                                         <  2:2756196/150‑1 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGATTTA‑AAAAAAAAATGTAatta                                                                                                                                                    >  2:2147503/1‑150 (MQ=255)
       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTG‑TTTTCGATTTA‑AAAATATAATGTAattat                                                                                                                                                   >  2:793541/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAattatt                                                                                                                                                  <  2:4173377/150‑1 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGATTTA‑AAAATATAAATAAattatt                                                                                                                                                  >  2:1658964/1‑150 (MQ=255)
         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGATTTC‑AAAATATAATGAAAATAttt                                                                                                                                                 >  2:1638920/1‑150 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTg                                                                                                                                                <  2:1342413/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTg                                                                                                                                                <  2:710746/150‑1 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGt                                                                                                                                               <  2:2437922/150‑1 (MQ=255)
            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGtt                                                                                                                                              <  2:3283894/150‑1 (MQ=255)
            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGtt                                                                                                                                              <  2:3283940/150‑1 (MQ=255)
              tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGtttt                                                                                                                                            <  2:3569604/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGttttt                                                                                                                                           <  1:3854856/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACATACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTa                                                                                                                                          <  2:3546794/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAa‑ta                                                                                                                                     <  1:1408926/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAa‑ta                                                                                                                                     <  1:4622173/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAa‑ta                                                                                                                                     <  2:1098954/150‑1 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAa‑tat                                                                                                                                    <  1:3430976/150‑1 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGATTTA‑AAAAAATAAATAAATAATTTTTTTTTAAa‑aat                                                                                                                                    >  1:2712299/1‑150 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGATTTA‑AAAATAAAAATAAAATATTTTTTTTTAAa‑tat                                                                                                                                    >  2:82879/1‑150 (MQ=255)
                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCgag                                                                                                                                <  1:552607/150‑1 (MQ=255)
                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGTTTTA‑AAAAAAAAAATAAATTATTTTTTTTTTAa‑tatatag                                                                                                                                >  1:2473395/1‑146 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATaa                                                                                                                            <  1:1272623/150‑1 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATaa                                                                                                                            <  1:4175409/150‑1 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAAc                                                                                                                           <  2:364280/150‑1 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATACCg                                                                                                                          <  1:1986154/150‑1 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACg                                                                                                                          <  2:4506432/150‑1 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACg                                                                                                                          <  2:1370862/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGAATTC‑AAAATAAAATTAAAATATTTTTTTTTAAA‑TATAGAGATAAAGAt                                                                                                                        >  2:2247393/1‑150 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTT‑TTTTCGATTTA‑AAAATATTATTAAAATATTTTTTTTTTAA‑TATAGAGATAACCATc                                                                                                                       >  2:3022389/1‑150 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTT‑TTTTATTTTTG‑AAAATATAATTTAATTATTTTTTTTTAAA‑TATAGATATaaacata                                                                                                                       >  2:3022383/1‑145 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTG‑AAAATATAAATAAATTATTTGTTTTTTAA‑TATCGAGATAACGCTc                                                                                                                       >  2:4529536/1‑150 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGATTTC‑AAAATATAATGAAATTATTTTTTTTTTAA‑TATAGATATaaaaata                                                                                                                       >  2:4529281/1‑145 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑AATAGAGATAACGATc                                                                                                                       >  2:3022367/1‑150 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATcac‑a                                                                                                                   <  1:4518244/150‑1 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGATTTA‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATAGAGATAAAGATcac‑a                                                                                                                   >  2:3508692/1‑150 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAGACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATcac‑a                                                                                                                   <  1:847443/150‑1 (MQ=255)
                                       tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTGGATTTA‑AAAATATAAATAAAATATTTTTTTTTTAA‑TATAGAGGTAAAAATAAC‑aaa                                                                                                                 >  1:3100209/1‑150 (MQ=255)
                                       tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑aaa                                                                                                                 <  2:9820/150‑1 (MQ=255)
                                       tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑GTTTGGATTTT‑AAAATATAATGAAAATATTTTTTTTTTAA‑TATAGAGATTACGATCAC‑aaa                                                                                                                 >  1:3378027/1‑150 (MQ=255)
                                       agAATGGCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTCTTAAA‑TATCGAGATAACGATCAC‑aaa                                                                                                                 <  2:2401356/149‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑aaaaa                                                                                                               <  2:2921667/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTGGTTTTT‑AAAATAAAAATAAAATATTTTTTTTTTAA‑TATAGTGATAAAAATAAC‑aaaaa                                                                                                               >  1:2943605/1‑150 (MQ=255)
                                           tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGATTTC‑AAAATATAATTAAATTATTTGTTTTTTAA‑TATAGTGTTAAAGATCAC‑aaaaaag                                                                                                             >  2:2872050/1‑148 (MQ=255)
                                             ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCTATTTA‑AAAATATAATTAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGAc                                                                                                           <  2:33034/150‑1 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGATTTA‑AAAATAAAATGAAATTATTTGTTTTTAAA‑TATAGAGATAACGATCAC‑AAAAACGaaa                                                                                                          >  2:3440844/1‑148 (MQ=255)
                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTGGTTTTC‑ATAAAATAAAGTAAAAATTTTTTTTTTTA‑TATAGTGATAAACAACAC‑AAAAAAGACaa                                                                                                         >  1:2102703/1‑150 (MQ=255)
                                                     gCATAACCGCCGCGCTGTGCGTTTTTCTGCATGTGCTTTGTCGATACCACGTACATTTTCTTATCCTGTCGTTTG‑TTTTCTTTTTC‑AAAATATAATTAAATTATTTGTTTTTAAA‑TATCGAGATAACTATCAC‑AAAAACGACAATATGaa                                                                                                   <  2:4171617/150‑1 (MQ=255)
                                                     gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGaa                                                                                                   <  2:2365799/150‑1 (MQ=255)
                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTT‑TTTTTGATTTA‑AAAAAATAAATAAAATATTTTTTTTTAAA‑TATAGAGATAAACATACA‑AAAAACCACAAAATGaaaa                                                                                                 >  2:572249/1‑150 (MQ=255)
                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTAGATTTC‑AAAATATAATGTAATTATTTTTTTTTTAA‑TATAGAGATAACGATCAA‑AAAAAAGACAATATGAAAat                                                                                                >  2:4309910/1‑150 (MQ=255)
                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGATTTA‑AAAATATAATTAAATTATTTTTTTTTAAA‑AATAGAGATAACCCTAAC‑AAAAACGACAAAATTAAAat                                                                                                >  2:1335857/1‑150 (MQ=255)
                                                            cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTGGATTTA‑AAAAAAAAATTAAAATATTTTTTTTTTAA‑AAAATAGGTAACGATAACAAAAAACAACAAAATCAAAattat                                                                                             >  1:4352631/1‑150 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTT‑TTTTAGATTTC‑AAAATATAATGAAAATATTTTTTTTTTTA‑TATATAGATAAAGATAAC‑AAAAAAGAAAAAATGAAAattatta                                                                                           >  2:4081506/1‑149 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTT‑TTTTAGAATTA‑AAAAAAAAATGAAAATATTTTTTTTTAAA‑AATAGAGATAAAAATAAC‑AAAAAACACAAAATTAAAaatatta                                                                                           >  2:1034741/1‑149 (MQ=255)
                                                                gcgcTGTGCGTTTTTCAGCATCTGCTTTGTCGATACCACTTACATTTTAATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAATTTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCgag                                                                                        <  2:2236514/150‑1 (MQ=255)
                                                                        cGTTGTTCAGCATGTGCTTTGTCTATACCACGAAATTTTTATTATCCTGTGGTTTT‑TTTTGGTTTTA‑AAAATATAATTAATTTTTTTTTTTTTAAA‑TATCGATATAACGAACAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGaaa                                                                               <  1:4183096/150‑1 (MQ=255)
                                                                           tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGTTTTA‑AAAAAATAAATAAAATAATTTTTTTTTAA‑TATAGATATAAACAAAAC‑AAAAAAAACAAAATTAAAAATATTTGAGGAG‑TTTAAGGc                                                                            >  1:284405/1‑150 (MQ=255)
                                                                             ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTG‑TTTTAGATTTA‑AAAAAATAATGAAATTATTTTTTTTTTAA‑TATCGAGATAAAGATAAC‑AAAAACGAAAATATTAAAATTATTAGATGAG‑TGAAATGCaa                                                                          >  2:3579749/1‑150 (MQ=255)
                                                                              tCAGAATCTTTTTTGTCTAAACCACTTAAATTTAAATATCCTGTTTTTTT‑TTTTCTATTAA‑AAAATATAATTAATTTTTTTTTTTTAAAT‑TATCTATAAAACTAAAAA‑AAACACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCaaa                                                                         <  1:3267233/150‑1 (MQ=255)
                                                                                aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTGGATTTG‑AAAATATAATGAAATTATTTTTTTTTTAA‑TATAGATATAAAGATAAC‑AAAAAAAACAAAAATAAAATTATTTGAGGGG‑TGTAAGGGaaaaa                                                                       >  2:2009255/1‑150 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTA‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCTAGATAACCATAAC‑AAAAAAGACAAAATTAAAATTATTCGAGGAT‑TGAAATGCaaaaaaaag                                                                   >  2:401805/1‑148 (MQ=255)
                                                                                        cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTGGATTTC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAAAGATAAC‑AAAAAAGACAATATTAAAATTATTGGTGGAGTTTAAGGGCAAAAAAACgcca                                                                >  2:2708306/1‑147 (MQ=255)
                                                                                        cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTT‑TTTTAGATTTA‑AAAAAATAATTAAAATATTTTTTTTTTAA‑TAAAGAGATAAAGATCAC‑AAAAAAGAAAAAATGTAAATTATTATAGGAG‑TGTAAGGCaaaaaaaaaggcg                                                               >  2:4049904/1‑144 (MQ=255)
                                                                                          ttGTCTATACAACGTAATTTTTAATATCGTTTGTTTTT‑TTTTCTTTTTA‑AAAATATATTTAATTTTTTTTTTTTTAAA‑TATCTATATAACTATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTcc                                                             <  2:1647896/150‑1 (MQ=255)
                                                                                           tgacGATACCACTTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATTAATTTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTccc                                                            <  2:2343378/147‑1 (MQ=255)
                                                                                           tGTCCACACCACCTACATTTTCATATCCTGTGTTTTG‑TTTTCTATTTA‑AAAATATAATTAATTTATTTGTTTTTAAA‑TATCGAGAAAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTccc                                                            <  2:2947016/150‑1 (MQ=255)
                                                                                           ggtctacaCCACGTACATTTACATATCCTGTCGTTTG‑TTTTCTATTAC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTccc                                                            <  2:2342232/143‑1 (MQ=255)
                                                                                                aaaCCACTTACTTTTTAATAACCTGTCGTTTG‑TTTTCGATTTA‑AAAATATAATCAATTTATTTTTTTTTAAA‑TATCGAGATAACTATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAg                                                       <  2:2471852/148‑1 (MQ=255)
                                                                                                aTACCACTTACATTTTAATTTCCTTTCGTTTT‑TTTTCTATTTC‑AAAATATAATTAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAg                                                       <  2:2471845/150‑1 (MQ=255)
                                                                                                 tACCACTTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  2:3107820/150‑1 (MQ=255)
                                                                                                 tACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCTATTTA‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  2:3107848/150‑1 (MQ=255)
                                                                                                  aCCACGTACATTTTCATATCCTGTCGTTTT‑TTTTAGATTTA‑AAAATATAAATAAAATAATTTTTTTTAAA‑TATAGATATAACAATAAC‑AAAAAAAAAAATAATAAAAATATTAGAGGAG‑TGAAATGCAAAAAAAATGCCACCCGAAAggg                                                     >  2:4771283/1‑150 (MQ=255)
                                                                                                  aCCACGTACAGTTTCATATCCTGTCGTTTG‑TTTTAGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATAGAGATAACGATAAC‑AAAAAACAAAAAAATAAAAATATTCGAGGGG‑TGAAATGGAAAAAAAAGGCCACCCGaaatgg                                                     >  2:4771282/1‑145 (MQ=255)
                                                                                                         aaaTTTTAATTTCTTGTC‑TTTT‑TTTTCATTTTAAAAAATATAAT‑AAATTTTTTTTTTTAAAATTACCTAGAAAACCATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                                              <  1:3722270/148‑1 (MQ=255)
                                                                                                                cATATCCTTTCGTTTG‑TTTTCTATTAC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                                       <  2:3803089/150‑1 (MQ=255)
                                                                                                                    tCCTGTCTTTTG‑TTTACGATTAA‑AAAATATAATTAATTTATTTTTTTTTAAA‑TATCTATATAACTACCAA‑AACCACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                   <  1:2300357/150‑1 (MQ=255)
                                                                                                                    tCCTGTCGTTTG‑TTTTCTATTAA‑AAATTAAAATGAAATTATTTGTTTTTAAA‑TACCGATATAACTAAAAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                   <  1:4698544/150‑1 (MQ=255)
                                                                                                                     tcgtttgtttgtttTTCTATTAA‑AAATTATATTTAATTTTTTTTTTTTTAAA‑TATCTATAAAAAGAAAAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                   <  1:151132/144‑1 (MQ=255)
                                                                                                                       tGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAAAGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                >  2:4230851/1‑150 (MQ=255)
                                                                                                                        gTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                               >  2:642447/1‑150 (MQ=255)
                                                                                                                         tCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AATAACGACAATATGAATATTATTCGAGGTG‑TGTAAGGCATATTTTCGGCCTCCCGATTGGGAAGCGGTAGCAAAGTGCGCgtgt                              >  2:2780553/1‑150 (MQ=255)
                                                                                                                            tttt‑ttttCGATTTC‑AAATTATAATTAATTTTTTTTTTTTTAAA‑TATCGAGATAACTAACAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           <  1:703983/150‑1 (MQ=255)
                                                                                                                            tttg‑tttTCTATTAC‑AAAATATAATTAATTTATTTGTTTTTAAA‑TATCGAGATAACTAACAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           <  1:1897758/150‑1 (MQ=255)
                                                                                                                            tttg‑tttTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCTAAGTGCGCGTGtttt                           >  1:1114436/1‑150 (MQ=255)
                                                                                                                            tttg‑tttTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           >  2:3935929/1‑150 (MQ=255)
                                                                                                                             ttg‑tttTCTATTAC‑AAAATATAATTAATTTTTTTGTTTTTAAA‑TATCGATATAACTATCAA‑AAACACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  1:298368/150‑1 (MQ=255)
                                                                                                                             ttg‑tttTCGATTTC‑AAAATATAATTAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  2:2218558/150‑1 (MQ=255)
                                                                                                                              tg‑tttTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         >  2:1959418/1‑150 (MQ=255)
                                                                                                                                 ttttCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACACTATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  2:1008512/1‑150 (MQ=255)
                                                                                                                                  tttCGATTTC‑AAAATATAATGAAATTTTTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACTGCCTCCCGATAGGGATGCCGTAGCACAGTGCGCGTGTTTTTATGc                      <  1:3569600/150‑1 (MQ=255)
                                                                                                                                   ttCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:309875/1‑150 (MQ=255)
                                                                                                                                   ttCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  1:3965806/1‑150 (MQ=255)
                                                                                                                                    tCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    >  2:3432939/1‑150 (MQ=255)
                                                                                                                                     ctttttA‑AAAATATATTAAATTTTTTTTTTTTTAAA‑TAGGGATATAACGAAAAC‑AAAAACTACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                   <  1:1274561/147‑1 (MQ=255)
                                                                                                                                      taTTTA‑AAAAAATAATTAAATTATTTTTTTTTAAT‑TATCTTTATAACTAAAAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  <  1:1757167/149‑1 (MQ=255)
                                                                                                                                      atattaaaaaaTATATTTAAATTATTTTTTTTTAAA‑TATCTATAAACCTAACAC‑AAACACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                   <  1:1276204/143‑1 (MQ=255)
                                                                                                                                              aaaTATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  1:1433320/1‑150 (MQ=255)
                                                                                                                                                       gAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  2:1055292/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG‑TTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑