BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,237,098	G→T	89.7%	H12N (CAT→AAT)	folE ←	GTP cyclohydrolase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,237,098	0	G	T	89.7%	622.2 / 66.6	224	H12N (CAT→AAT)	folE	GTP cyclohydrolase I
Reads supporting (aligned to +/- strand): ref base G (11/12); new base T (100/101); total (111/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

Only 100 of 224 total aligned reads displayed.

TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA  >  CP009273/2236951‑2237243

tCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTaa                                                                                                                                                 <  1:2289763/150‑1 (MQ=255)

   aaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCa                                                                                                                                              <  1:3626491/150‑1 (MQ=255)

    aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                             >  1:3370229/1‑150 (MQ=255)

    aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                             >  1:1555284/1‑150 (MQ=255)

     cTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAgg                                                                                                                                            >  1:4250076/1‑150 (MQ=255)

       gTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGc                                                                                                                                          <  2:4784856/150‑1 (MQ=255)

           tcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGct                                                                                                                                      >  2:64834/1‑150 (MQ=255)

            caGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGctt                                                                                                                                     <  2:2396199/150‑1 (MQ=255)

             aGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttc                                                                                                                                    >  2:1371027/1‑150 (MQ=255)

                 aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTa                                                                                                                                <  2:1747146/150‑1 (MQ=255)

                      gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAg                                                                                                                           >  1:2748637/1‑150 (MQ=255)

                      gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAg                                                                                                                           <  1:89188/150‑1 (MQ=255)

                      gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAg                                                                                                                           <  1:2080885/150‑1 (MQ=255)

                      gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAg                                                                                                                           >  2:3464624/1‑150 (MQ=255)

                           tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATg                                                                                                                      <  2:1136666/150‑1 (MQ=255)

                            cagcagCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATgg                                                                                                                     <  2:91541/150‑1 (MQ=255)

                               cagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAt                                                                                                                  <  1:2901052/150‑1 (MQ=255)

                                agcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGATGGCAtt                                                                                                                 >  2:3740477/1‑150 (MQ=255)

                                 gcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAttt                                                                                                                >  2:4390795/1‑150 (MQ=255)

                                  cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTa                                                                                                               >  2:1293393/1‑150 (MQ=255)

                                   tGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAt                                                                                                              <  1:4094720/150‑1 (MQ=255)

                                    gCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATg                                                                                                             <  1:193465/150‑1 (MQ=255)

                                    gCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAGTTATg                                                                                                             >  2:2184469/1‑150 (MQ=255)

                                    gCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAAAGAGTGGTTGGATTTATg                                                                                                             >  1:73001/1‑150 (MQ=255)

                                     cATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGa                                                                                                            <  1:1155034/150‑1 (MQ=255)

                                      aTGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAt                                                                                                           <  1:345072/150‑1 (MQ=255)

                                      aTGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAt                                                                                                           <  1:3595014/150‑1 (MQ=255)

                                        gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATCCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                         <  1:4486956/150‑1 (MQ=255)

                                         aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTGTTCTGAGTGATTGCAGTTATGAttta                                                                                                        >  1:652760/1‑149 (MQ=255)

                                            tCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTcc                                                                                                     >  2:696854/1‑150 (MQ=255)

                                              ggTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                                                                   <  1:4203011/150‑1 (MQ=255)

                                              ggTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                                                                   >  2:3151786/1‑150 (MQ=255)

                                                tCATAGGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCa                                                                                                 <  2:2589428/150‑1 (MQ=255)

                                                   tatGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgt                                                                                              >  1:1092086/1‑150 (MQ=255)

                                                        ccAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGCATTTATGATTTCTCCTGCAGGTGTGAc                                                                                         >  1:4719916/1‑150 (MQ=255)

                                                           gCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGcc                                                                                      <  2:2209586/150‑1 (MQ=255)

                                                            cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCt                                                                                     <  2:200566/150‑1 (MQ=255)

                                                            cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCt                                                                                     <  1:386520/150‑1 (MQ=255)

                                                            cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCATCAGGTGTGACGccg                                                                                     >  1:1784412/1‑149 (MQ=255)

                                                            cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAAATAGTGATTGGATTTATGATTTCTCCAGAAGGTGTGGCGcca                                                                                     >  1:4453225/1‑149 (MQ=255)

                                                             aaTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGTTGGCAGTTATGATTTATCCAGCAGGTGTGACGccac                                                                                    >  1:2190468/1‑148 (MQ=255)

                                                                aaGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGc                                                                                 >  2:528091/1‑150 (MQ=255)

                                                                  ggCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTAGTTACTGAGTGATGGCATTTATGAATTCTCCTGCAGGTGTGACGCCACCGccc                                                                               >  2:1257231/1‑150 (MQ=255)

                                                                   gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTTCTGAGTGTTGGCAGTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                              >  2:118843/1‑150 (MQ=255)

                                                                    cTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCACCGCCCTg                                                                             >  2:485856/1‑150 (MQ=255)

                                                                    cTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCGTTACTGAGTGATGGCAGTTATTATTTATACTGCAGGTGTGACGCCTCCGCCCTg                                                                             >  1:2520455/1‑150 (MQ=255)

                                                                      tttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGTGTGTTGGCATTTATGATTTCTCCTGCCGGTGTGACGCCACCGCCCTGCg                                                                           >  2:580588/1‑150 (MQ=255)

                                                                        tGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTg                                                                         <  1:177242/150‑1 (MQ=255)

                                                                              tttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCa                                                                   <  1:4741907/150‑1 (MQ=255)

                                                                              tttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCa                                                                   <  2:437942/150‑1 (MQ=255)

                                                                               ttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCaa                                                                  <  2:3791131/150‑1 (MQ=255)

                                                                                tCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCaaa                                                                 <  1:3339412/150‑1 (MQ=255)

                                                                                 cGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAg                                                                <  1:985003/150‑1 (MQ=255)

                                                                                  gTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGGGGGGGCAAAGt                                                               >  1:1565519/1‑150 (MQ=255)

                                                                                        cATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGGATTTTTGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaa                                                         >  1:776000/1‑150 (MQ=255)

                                                                                        cATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaa                                                         <  2:4250278/150‑1 (MQ=255)

                                                                                        cATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaa                                                         <  2:4208038/150‑1 (MQ=255)

                                                                                         aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGGAAAGTTATTAAg                                                        >  2:2047588/1‑150 (MQ=255)

                                                                                         aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAg                                                        <  2:3696444/150‑1 (MQ=255)

                                                                                         aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAg                                                        <  1:3635576/150‑1 (MQ=255)

                                                                                          tttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGc                                                       <  1:1662910/150‑1 (MQ=255)

                                                                                          tttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCGTTACAGAGTGATGGCCGTTATGATTTCTCCTGCAGGGGTGACGCCACCGCCCAGCGGGGGGGCCAAGTTATTAAGc                                                       >  1:2539684/1‑150 (MQ=255)

                                                                                           ttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAATGAGTGGTGGCATTTATGATTTATCCTGCAGGTGTGTCGCCTCCGCCCGGCGGGGGGGCCAAGGTATTAAGCt                                                      >  2:817706/1‑150 (MQ=255)

                                                                                            tCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTg                                                     <  1:935518/150‑1 (MQ=255)

                                                                                               tGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAtt                                                  <  1:3741661/150‑1 (MQ=255)

                                                                                                gCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAttt                                                 >  2:3074245/1‑150 (MQ=255)

                                                                                                  aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAc                                               <  1:1628760/150‑1 (MQ=255)

                                                                                                   cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACa                                              <  2:2997501/150‑1 (MQ=255)

                                                                                                   cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAATGAGTGGTGGCCTTTTTGATTTCTCCAGCAGGTGTGACGCCGCCCCCCAGCGGGGGGGCCAAGTTATTAAGCTGATTTACa                                              >  1:1025387/1‑150 (MQ=255)

                                                                                                     ggcgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGTTATTAATCTGATTTAAAGc                                            >  2:2846539/1‑150 (MQ=255)

                                                                                                     ggcgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATGTCTCCTGCCGGTGTGACGCCTCCGCCCGGCGGGGGGGCACAGTTATTAAGCTGATTTAAAGc                                            >  2:2846506/1‑150 (MQ=255)

                                                                                                        gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCCTTTATGATTTATCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGCACAGTTATTAAGCTGATTTACAGccag                                         >  2:443705/1‑148 (MQ=255)

                                                                                                         ggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGa                                        <  1:3979179/150‑1 (MQ=255)

                                                                                                         ggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGa                                        <  1:1571867/150‑1 (MQ=255)

                                                                                                           cgcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatt                                      <  1:1371027/150‑1 (MQ=255)

                                                                                                           cgcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGGTTACTGAGTGGTGGGCTTTATGTTTTATCCTGCAGGGGTGACGCCACCGCCCTGCGTGGGGGCCCAGTTATTAAGATGTTTTACAGCCTGatt                                      >  1:1364240/1‑150 (MQ=255)

                                                                                                              aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattatt                                   <  2:4456640/150‑1 (MQ=255)

                                                                                                              aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattatt                                   <  2:1897424/150‑1 (MQ=255)

                                                                                                              aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCAGTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGGTATTAAGCTGATTTACAGCCAGattatt                                   >  2:3232779/1‑150 (MQ=255)

                                                                                                              aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattatt                                   <  2:2924552/150‑1 (MQ=255)

                                                                                                                   tgtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTATTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCAGATTTACAGCCAGATTATTGTGCg                              >  2:4095280/1‑150 (MQ=255)

                                                                                                                      ttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCGGCGTGGGGGCAAAGTTATTAATCTGATTTAAAGCCTGATTATTTTTCGTGa                           >  1:294161/1‑150 (MQ=255)

                                                                                                                      ttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGATTGCATTTATGATTTATCCAGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGTTATTAATCTGATTTAAAGCCAGATTATTTTTCGTGa                           >  1:294036/1‑150 (MQ=255)

                                                                                                                      ttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGTGTGTTTGCATTTATTTTTTATCCTGCAGGTGTGACGCCAACCCCCAGCGGGGGGGCAAAGTTATTAAACAGTTTTTAAGCCATATTATTTTGAGTgt                           >  1:935128/1‑149 (MQ=255)

                                                                                                                      ttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGATGGGATTTATGATTTATCCAGCAGGTGTGACGCCACCCCCCTGCGTGGGGGGAAAGTTATTAAGCTGATTTACAGCCAGATTATTGTGCGTgt                           >  1:2233506/1‑149 (MQ=255)

                                                                                                                         cAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACGGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGCCAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggc                        >  1:2391222/1‑150 (MQ=255)

                                                                                                                          aGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAATCTGATTTACAGCCTGATTATTTTGCGTGAggcg                       >  2:1799401/1‑150 (MQ=255)

                                                                                                                          aGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcg                       <  1:531731/150‑1 (MQ=255)

                                                                                                                          aGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGGTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcg                       >  2:1582460/1‑150 (MQ=255)

                                                                                                                                cgcgCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGcac                 <  2:3370229/150‑1 (MQ=255)

                                                                                                                                cgcgCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGcac                 <  2:4008689/150‑1 (MQ=255)

                                                                                                                                  cgcAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAt               <  2:2350314/150‑1 (MQ=255)

                                                                                                                                     aaCTAACGCTTCATTAACCAGGGCCGCTTGTTTAATGAGTGATTGGCTTTATGATTTCTCCAGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCAGATTATTTTTCGTGAGGCGGCGCACAAAAt            >  1:4323327/1‑150 (MQ=255)

                                                                                                                                       cTAACGCTTCATTAACCAGGGCCGCTTATTTACATTGTGATGGCATTTATGTTTTATACTGCAGGTGTGAACCCTCCCCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAAAGCCTGATTATTGTTAGGGAGGCGGCGCACaaaaaac          >  2:1601804/1‑147 (MQ=255)

                                                                                                                                        tAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCa         <  2:2474093/150‑1 (MQ=255)

                                                                                                                                        tAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCa         <  2:2474024/150‑1 (MQ=255)

                                                                                                                                           cGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTa      <  2:1615688/150‑1 (MQ=255)

                                                                                                                                            gCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAt     <  1:1039041/150‑1 (MQ=255)

                                                                                                                                             cTTCATTAACCAGGGCCGCTTCTTTACATAGTGTTGGCATTTATTTTTTCTCCAGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTTTGCGTGAGGCGGCGCACATAATACCGTAtt    >  2:2065518/1‑150 (MQ=255)

                                                                                                                                               tCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTca  <  1:3192324/150‑1 (MQ=255)

TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA  >  CP009273/2236951‑2237243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`