Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,373,129 C→A 32.9% D332Y (GAT→TAT)  menF ← isochorismate synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,373,1290CA32.9% 529.6 / 279.0 300D332Y (GAT→TAT) menFisochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (108/93);  new base A (41/58);  total (149/151)
Fisher's exact test for biased strand distribution p-value = 4.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

TCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCG  >  CP009273/2372981‑2373276
                                                                                                                                                    |                                                                                                                                                   
 cGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatcat                                                                                                                                                   >  1:2868494/1‑150 (MQ=255)
 cGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatcat                                                                                                                                                   >  1:1529496/1‑150 (MQ=255)
   cTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATcc                                                                                                                                                 >  1:3624702/1‑150 (MQ=255)
   cTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATcc                                                                                                                                                 <  1:595854/150‑1 (MQ=255)
    tttGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCg                                                                                                                                                >  2:398875/1‑150 (MQ=255)
      tgttgtAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCt                                                                                                                                              <  1:3350595/150‑1 (MQ=255)
        ttgtAATGGGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCttt                                                                                                                                            >  2:706682/1‑150 (MQ=255)
           tAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATACACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTACACAGATCACATAATCCGCTttgtt                                                                                                                                         >  1:233270/1‑150 (MQ=255)
           tAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTttgtt                                                                                                                                         <  2:2500290/150‑1 (MQ=255)
            aaTGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTttgttg                                                                                                                                        >  2:2144136/1‑150 (MQ=255)
            aaTGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTttgttg                                                                                                                                        >  2:1198721/1‑150 (MQ=255)
            aaTGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTttgttg                                                                                                                                        >  1:457381/1‑150 (MQ=255)
             aTGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGa                                                                                                                                       <  2:4568357/150‑1 (MQ=255)
             aTGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGa                                                                                                                                       <  1:2807146/150‑1 (MQ=255)
             aTGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGa                                                                                                                                       <  2:1849884/150‑1 (MQ=255)
               gagagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGt                                                                                                                                     <  2:4089035/150‑1 (MQ=255)
               gagagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGt                                                                                                                                     >  1:1186292/1‑150 (MQ=255)
                agagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTg                                                                                                                                    >  2:4186860/1‑150 (MQ=255)
                 gagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGa                                                                                                                                   >  1:2041124/1‑150 (MQ=255)
                 gagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGa                                                                                                                                   >  1:4261117/1‑150 (MQ=255)
                        cccGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAg                                                                                                                            >  2:4721718/1‑150 (MQ=255)
                           gCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATa                                                                                                                         <  1:4181880/150‑1 (MQ=255)
                             aGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACa                                                                                                                       >  2:4425778/1‑150 (MQ=255)
                              gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                      >  2:2609997/1‑150 (MQ=255)
                                 ccGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAgcgg                                                                                                                   <  2:3975694/150‑1 (MQ=255)
                                  cGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAgcggc                                                                                                                  >  2:3239215/1‑150 (MQ=255)
                                  cGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAgcggc                                                                                                                  >  2:850226/1‑150 (MQ=255)
                                   ggCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAgcggcg                                                                                                                 >  1:3128519/1‑150 (MQ=255)
                                      gTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAg                                                                                                              <  2:879964/150‑1 (MQ=255)
                                       tACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGa                                                                                                             >  2:959457/1‑150 (MQ=255)
                                         ccATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtg                                                                                                           >  2:63948/1‑150 (MQ=255)
                                         ccATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtg                                                                                                           >  2:3181204/1‑150 (MQ=255)
                                            ttCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgctg                                                                                                        <  1:1929771/150‑1 (MQ=255)
                                             tctcGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgctgc                                                                                                       >  1:869592/1‑150 (MQ=255)
                                              ctcGTGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCa                                                                                                      <  1:2595809/150‑1 (MQ=255)
                                              ctcGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCa                                                                                                      >  2:181421/1‑150 (MQ=255)
                                                     gAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacg                                                                                               <  1:1128408/150‑1 (MQ=255)
                                                     gAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacg                                                                                               <  1:4486159/150‑1 (MQ=255)
                                                     gAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacg                                                                                               >  1:3424876/1‑150 (MQ=255)
                                                     gAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacg                                                                                               <  1:398814/150‑1 (MQ=255)
                                                       aCGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgca                                                                                             >  1:2151756/1‑150 (MQ=255)
                                                        cGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcag                                                                                            >  2:1550089/1‑150 (MQ=255)
                                                          gTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcagac                                                                                          >  2:4671575/1‑150 (MQ=255)
                                                           ttCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcagacg                                                                                         <  1:1493619/150‑1 (MQ=255)
                                                                gACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTa                                                                                    <  1:3052774/150‑1 (MQ=255)
                                                                gACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTa                                                                                    >  1:4330561/1‑150 (MQ=255)
                                                                gACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTa                                                                                    >  2:2607269/1‑150 (MQ=255)
                                                                 aCGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAc                                                                                   >  1:278399/1‑150 (MQ=255)
                                                                      cGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcg                                                                              >  2:3451033/1‑150 (MQ=255)
                                                                      cGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcg                                                                              >  1:2302709/1‑150 (MQ=255)
                                                                       gATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcgg                                                                             <  1:1869820/150‑1 (MQ=255)
                                                                        aTAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcggc                                                                            >  2:4362980/1‑150 (MQ=255)
                                                                                tCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAAc                                                                    <  2:3599228/150‑1 (MQ=255)
                                                                                   cgcCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATc                                                                 >  2:4201615/1‑150 (MQ=255)
                                                                                     ccAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCa                                                               <  1:1570156/150‑1 (MQ=255)
                                                                                           cgcgcgGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCt                                                         <  2:4134636/150‑1 (MQ=255)
                                                                                           cgcgcgGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCt                                                         <  2:2041124/150‑1 (MQ=255)
                                                                                             cgcgGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTgg                                                       <  1:1640606/150‑1 (MQ=255)
                                                                                               cgGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGt                                                     >  1:4426457/1‑150 (MQ=255)
                                                                                                ggTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTa                                                    >  2:3875607/1‑150 (MQ=255)
                                                                                                   aaGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCg                                                 >  1:1806477/1‑150 (MQ=255)
                                                                                                   aaGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGAGTCTGGGTATCg                                                 <  2:575763/150‑1 (MQ=255)
                                                                                                    aGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCgg                                                >  1:466532/1‑150 (MQ=255)
                                                                                                    aGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCgg                                                >  2:4766982/1‑150 (MQ=255)
                                                                                                     gCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGc                                               <  1:383614/150‑1 (MQ=255)
                                                                                                      ccAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCGTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTGTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGcc                                              >  1:3236250/1‑150 (MQ=255)
                                                                                                      ccAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGcc                                              <  1:4593675/150‑1 (MQ=255)
                                                                                                       cAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCt                                             >  1:2379910/1‑150 (MQ=255)
                                                                                                        aGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTg                                            >  1:48528/1‑150 (MQ=255)
                                                                                                          cAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTa                                          <  2:1484658/150‑1 (MQ=255)
                                                                                                          cAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTa                                          <  1:254996/150‑1 (MQ=255)
                                                                                                           aaCTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTaa                                         >  1:3382891/1‑150 (MQ=255)
                                                                                                            aCTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAAt                                        <  2:2702777/150‑1 (MQ=255)
                                                                                                              tgctgcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCg                                      >  1:1690695/1‑150 (MQ=255)
                                                                                                              tgctgcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCg                                      >  1:3902691/1‑150 (MQ=255)
                                                                                                                 tgcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTg                                   >  1:2681270/1‑150 (MQ=255)
                                                                                                                 tgcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTg                                   <  1:527747/150‑1 (MQ=255)
                                                                                                                 tgcCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTg                                   >  1:4408613/1‑150 (MQ=255)
                                                                                                                  gcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGa                                  <  1:2132668/150‑1 (MQ=255)
                                                                                                                   ccGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGAc                                 <  1:1001433/150‑1 (MQ=255)
                                                                                                                     gTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAg                               <  2:2534284/150‑1 (MQ=255)
                                                                                                                       tGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGat                             <  2:2868494/150‑1 (MQ=255)
                                                                                                                       tGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGat                             <  1:921006/150‑1 (MQ=255)
                                                                                                                          cTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATc                          <  2:1684039/150‑1 (MQ=255)
                                                                                                                           tGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCt                         <  1:2203426/150‑1 (MQ=255)
                                                                                                                            gCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCtt                        <  1:4631235/150‑1 (MQ=255)
                                                                                                                            gCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCtt                        <  2:2313556/150‑1 (MQ=255)
                                                                                                                              aaCTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTcc                      >  1:339/1‑150 (MQ=255)
                                                                                                                              aaCTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTcc                      >  2:2403952/1‑150 (MQ=255)
                                                                                                                                cTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                    <  1:4046416/150‑1 (MQ=255)
                                                                                                                                cTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                    <  1:651502/150‑1 (MQ=255)
                                                                                                                                 tGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacc                   >  2:306005/1‑150 (MQ=255)
                                                                                                                                  gATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacca                  <  1:1006536/150‑1 (MQ=255)
                                                                                                                                  gATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacca                  <  2:2940653/150‑1 (MQ=255)
                                                                                                                                     gTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccacca               >  1:3691804/1‑150 (MQ=255)
                                                                                                                                      tAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAg              <  2:1389375/150‑1 (MQ=255)
                                                                                                                                          cAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATg          <  1:136819/150‑1 (MQ=255)
                                                                                                                                           aGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGt         >  1:2959277/1‑150 (MQ=255)
                                                                                                                                           aGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGt         >  2:4292061/1‑150 (MQ=255)
                                                                                                                                              tCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtct      <  1:4116720/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCG  >  CP009273/2372981‑2373276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑