Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,379,450 C→T 5.1% P38S (CCT→TCT)  yfbL → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,379,4500CT 5.1% 641.8 / 14.3 177P38S (CCT→TCT) yfbLputative peptidase
Reads supporting (aligned to +/- strand):  ref base C (104/64);  new base T (6/3);  total (110/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 181 total aligned reads displayed.

TAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTCCCC  >  CP009273/2379302‑2379599
                                                                                                                                                     |                                                                                                                                                     
taatGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTcc                                                                                                                                                      >  2:3345123/1‑150 (MQ=255)
 aatGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCt                                                                                                                                                     <  1:4314584/150‑1 (MQ=255)
    gATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAg                                                                                                                                                  <  1:3090569/150‑1 (MQ=255)
     aTTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACAAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCACGTCCTGAGc                                                                                                                                                 >  1:3224609/1‑150 (MQ=255)
         aacaacGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAAtt                                                                                                                                             <  2:2189580/150‑1 (MQ=255)
              cGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGaaa                                                                                                                                        <  1:2161994/150‑1 (MQ=255)
              cGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGaaa                                                                                                                                        >  1:159251/1‑150 (MQ=255)
              cGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGaaa                                                                                                                                        <  1:2161766/150‑1 (MQ=255)
                 aaCCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGaaaaaa                                                                                                                                     >  1:3574657/1‑150 (MQ=255)
                 aaCCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGaaaaaa                                                                                                                                     >  2:3314713/1‑150 (MQ=255)
                  aCCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAAc                                                                                                                                    >  2:4114026/1‑150 (MQ=255)
                    ccATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACa                                                                                                                                   <  2:2539992/150‑1 (MQ=255)
                        taataaTTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGACAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg                                                                                                                              >  2:1849192/1‑150 (MQ=255)
                          ataatTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGtt                                                                                                                            >  1:1888527/1‑150 (MQ=255)
                           taatTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTa                                                                                                                           >  2:4464679/1‑150 (MQ=255)
                               ttGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCtt                                                                                                                       <  2:746111/150‑1 (MQ=255)
                               ttGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCtt                                                                                                                       <  1:433795/150‑1 (MQ=255)
                                 gCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTac                                                                                                                     >  1:1638989/1‑150 (MQ=255)
                                    tatG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTAcaca                                                                                                                  >  2:3626373/1‑150 (MQ=255)
                                       g‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAAc                                                                                                               <  2:2056374/150‑1 (MQ=255)
                                        taaaaaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAAc                                                                                                               <  1:3282734/149‑1 (MQ=255)
                                          aaaaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCCATTAGAAAAAACAGTTCTTTATCTTAAACAAACtg                                                                                                             >  2:4169791/1‑150 (MQ=255)
                                           aaaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACtgt                                                                                                            <  1:4103281/150‑1 (MQ=255)
                                            aaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGTGTCAATGCATTACAGTAAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCTTTATCTTAAACAAACtgtg                                                                                                           >  2:4298697/1‑150 (MQ=255)
                                            aaaaTAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACtgtg                                                                                                           >  1:2353486/1‑150 (MQ=255)
                                                 aatCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATcc                                                                                                      >  2:1581725/1‑150 (MQ=255)
                                                   tCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCAc                                                                                                    <  2:219531/150‑1 (MQ=255)
                                                   tCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCAc                                                                                                    <  2:219790/150‑1 (MQ=255)
                                                   tCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCAc                                                                                                    >  1:2515535/1‑150 (MQ=255)
                                                    cTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACg                                                                                                   <  1:1775841/150‑1 (MQ=255)
                                                    cTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACg                                                                                                   <  2:2914966/150‑1 (MQ=255)
                                                       tGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAg                                                                                                <  2:219187/150‑1 (MQ=255)
                                                       tGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAg                                                                                                <  2:860503/150‑1 (MQ=255)
                                                       tGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAg                                                                                                <  1:3403350/150‑1 (MQ=255)
                                                           tttATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGcc                                                                                            >  1:1875432/1‑150 (MQ=255)
                                                           tttATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGcc                                                                                            <  1:1958956/150‑1 (MQ=255)
                                                             tattatattaTTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGa                                                                                          >  2:2026442/1‑150 (MQ=255)
                                                              attatattatTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGAc                                                                                         <  1:3488769/150‑1 (MQ=255)
                                                              attatattatTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGAc                                                                                         >  1:2079044/1‑150 (MQ=255)
                                                                 atattatTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAat                                                                                      >  2:1304139/1‑150 (MQ=255)
                                                                    ttattTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTAAACAAACTGTGCATCCACGTAGTGCCGACAATAtt                                                                                   >  2:4427100/1‑150 (MQ=255)
                                                                      attTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGa                                                                                 <  2:1536046/150‑1 (MQ=255)
                                                                         tgtgTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATaa                                                                              <  2:2905907/150‑1 (MQ=255)
                                                                            ttttttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCt                                                                           <  2:2043552/149‑1 (MQ=255)
                                                                             tttttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTg                                                                          >  1:2885718/1‑150 (MQ=255)
                                                                              ttttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGa                                                                         <  1:4604206/150‑1 (MQ=255)
                                                                                ttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAAt                                                                       >  2:4523773/1‑150 (MQ=255)
                                                                                ttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAAt                                                                       >  2:3440130/1‑150 (MQ=255)
                                                                                ttACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAAt                                                                       >  2:1037642/1‑150 (MQ=255)
                                                                                 tACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATa                                                                      >  1:1164506/1‑150 (MQ=255)
                                                                                 tACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATa                                                                      >  2:3848552/1‑150 (MQ=255)
                                                                                  aCTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAg                                                                     >  1:428810/1‑150 (MQ=255)
                                                                                  aCTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAg                                                                     >  1:428844/1‑150 (MQ=255)
                                                                                   cTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAgg                                                                    >  1:4175878/1‑150 (MQ=255)
                                                                                   cTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAgg                                                                    >  1:236886/1‑150 (MQ=255)
                                                                                      ccTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTcc                                                                 >  2:4746596/1‑150 (MQ=255)
                                                                                      ccTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTcc                                                                 >  1:1515967/1‑150 (MQ=255)
                                                                                       cTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCg                                                                >  1:2438141/1‑150 (MQ=255)
                                                                                        tATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGc                                                               >  2:3084378/1‑150 (MQ=255)
                                                                                         aTGATTATTTTTTACCAACACTGGGTCAATGAAAAACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTACGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGCTAATCTGAATAGGTCCGCa                                                              >  2:727928/1‑150 (MQ=255)
                                                                                            attattTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGaa                                                           >  1:4395046/1‑150 (MQ=255)
                                                                                            attattTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGaa                                                           >  1:1232275/1‑150 (MQ=255)
                                                                                              tattTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGACTAGGTCCGCAGAata                                                         >  1:2846993/1‑150 (MQ=255)
                                                                                              tattTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAata                                                         >  2:3535265/1‑150 (MQ=255)
                                                                                              tattTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAata                                                         >  2:660710/1‑150 (MQ=255)
                                                                                              tattTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAata                                                         >  1:2846864/1‑150 (MQ=255)
                                                                                               attTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAatat                                                        >  2:168397/1‑150 (MQ=255)
                                                                                                 tttttACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAatatat                                                      >  1:535464/1‑150 (MQ=255)
                                                                                                     tACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATaaaa                                                  >  1:1609850/1‑150 (MQ=255)
                                                                                                      aCCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAag                                                 >  1:1210103/1‑150 (MQ=255)
                                                                                                       ccAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAaga                                                >  1:1320579/1‑150 (MQ=255)
                                                                                                       ccAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAaga                                                <  1:812426/150‑1 (MQ=255)
                                                                                                         aaCCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAgg                                              >  1:3628366/1‑150 (MQ=255)
                                                                                                              tGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCttt                                         <  2:2772355/150‑1 (MQ=255)
                                                                                                              tGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCttt                                         >  2:2776749/1‑150 (MQ=255)
                                                                                                              tGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCttt                                         >  1:3333600/1‑150 (MQ=255)
                                                                                                              tGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCttt                                         >  1:3334602/1‑150 (MQ=255)
                                                                                                              tGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCttt                                         <  2:380010/150‑1 (MQ=255)
                                                                                                              tGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGAAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCttt                                         >  1:1067770/1‑150 (MQ=255)
                                                                                                               gggTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTg                                        >  1:1110531/1‑150 (MQ=255)
                                                                                                                  tCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCa                                     <  1:2868456/150‑1 (MQ=255)
                                                                                                                  tCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCa                                     >  1:4437576/1‑150 (MQ=255)
                                                                                                                  tCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCa                                     <  2:4208346/150‑1 (MQ=255)
                                                                                                                   cAATGCATTACCGTCAACGCCCCGACACGCAAGTTCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAg                                    <  2:2518994/150‑1 (MQ=255)
                                                                                                                   cAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAg                                    <  1:1575517/150‑1 (MQ=255)
                                                                                                                     aTGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTa                                  >  2:2230424/1‑150 (MQ=255)
                                                                                                                     aTGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTa                                  >  2:2229015/1‑150 (MQ=255)
                                                                                                                      tGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAg                                 <  2:3871848/150‑1 (MQ=255)
                                                                                                                        cATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCg                               <  1:1055317/150‑1 (MQ=255)
                                                                                                                           tACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTg                            >  2:4076794/1‑150 (MQ=255)
                                                                                                                                  aaCGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTGTGTCAGTAGCGGTGCCAGAGt                     >  1:4272352/1‑150 (MQ=255)
                                                                                                                                   aCGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGtt                    <  2:1690785/150‑1 (MQ=255)
                                                                                                                                    cGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTa                   <  2:159251/150‑1 (MQ=255)
                                                                                                                                     gCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTAc                  >  1:4541139/1‑150 (MQ=255)
                                                                                                                                     gCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTAc                  <  2:1422907/150‑1 (MQ=255)
                                                                                                                                     gCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACCGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTAc                  >  2:2276966/1‑150 (MQ=255)
                                                                                                                                       cccGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCt                >  2:3955545/1‑150 (MQ=255)
                                                                                                                                                   gTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAAGAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTcc    >  2:2225153/1‑150 (MQ=255)
                                                                                                                                                     ccTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTcccc  <  1:3626373/150‑1 (MQ=255)
                                                                                                                                                     ccTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTcccc  <  1:1484783/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCGTTATCTTACACAAACTGTGCATCCACGTAGTGCCGACAATATTGATAATCTGAATAGGTCCGCAGAATATATAAAAGAGGTCTTTGTCAGTAGCGGTGCCAGAGTTACCTCGCAGGACGTCCCC  >  CP009273/2379302‑2379599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑