Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,516,553 T→G 10.6% E264D (GAA→GAC)  xapB ← xanthosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,516,5530TG10.6% 739.8 / 80.5 264E264D (GAA→GAC) xapBxanthosine transporter
Reads supporting (aligned to +/- strand):  ref base T (110/126);  new base G (14/14);  total (124/140)
Fisher's exact test for biased strand distribution p-value = 8.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 268 total aligned reads displayed.

AGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAG  >  CP009273/2516407‑2516702
                                                                                                                                                  |                                                                                                                                                     
agcagcagcAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCt                                                                                                                                                    <  1:2134080/150‑1 (MQ=255)
   agcagcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGcc                                                                                                                                                 <  2:1449028/150‑1 (MQ=255)
       gcaAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATCCTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCt                                                                                                                                             <  2:2532003/150‑1 (MQ=255)
        caAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCtg                                                                                                                                            <  1:4398289/150‑1 (MQ=255)
        caAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCtg                                                                                                                                            <  2:2094427/150‑1 (MQ=255)
             taaatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGaaa                                                                                                                                       >  2:2068540/1‑150 (MQ=255)
               aatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACt                                                                                                                                     <  1:1754174/150‑1 (MQ=255)
                atCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTg                                                                                                                                    <  1:2685816/150‑1 (MQ=255)
                  ccGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGAc                                                                                                                                  <  2:2785653/150‑1 (MQ=255)
                   cGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACa                                                                                                                                 <  1:2955127/150‑1 (MQ=255)
                   cGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACa                                                                                                                                 <  1:274070/150‑1 (MQ=255)
                    ggTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAg                                                                                                                                <  2:4288528/150‑1 (MQ=255)
                     gttgttGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGt                                                                                                                               >  1:384767/1‑150 (MQ=255)
                       tgttgACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTaa                                                                                                                             <  1:1115726/150‑1 (MQ=255)
                       tgttgACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTaa                                                                                                                             <  1:81652/150‑1 (MQ=255)
                        gttgACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTaaa                                                                                                                            <  2:3717154/150‑1 (MQ=255)
                            aCGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATgg                                                                                                                        <  1:4011489/150‑1 (MQ=255)
                            aCGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATgg                                                                                                                        >  2:1308972/1‑150 (MQ=255)
                               gATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAgg                                                                                                                     <  2:2447093/150‑1 (MQ=255)
                               gATCGCCATAGGCAAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAgg                                                                                                                     >  1:2460019/1‑150 (MQ=255)
                                  cGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGat                                                                                                                  <  1:3039151/150‑1 (MQ=255)
                                    ccATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGatat                                                                                                                <  2:1147741/150‑1 (MQ=255)
                                      aTAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATAttt                                                                                                              <  2:4752279/150‑1 (MQ=255)
                                        aGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTca                                                                                                            <  2:3039117/150‑1 (MQ=255)
                                         ggCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTcac                                                                                                           <  1:1942495/150‑1 (MQ=255)
                                          gCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTcacc                                                                                                          <  1:1422631/150‑1 (MQ=255)
                                           cGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTcacca                                                                                                         <  2:1229605/150‑1 (MQ=255)
                                            gaagaaGCCAAAGCGCAGCGTCCGGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTcaccac                                                                                                        >  1:3186413/1‑150 (MQ=255)
                                                aagCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACaaaa                                                                                                    >  1:970449/1‑150 (MQ=255)
                                                 agCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAAc                                                                                                   <  2:372230/150‑1 (MQ=255)
                                                   ccAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTg                                                                                                 >  1:3517463/1‑150 (MQ=255)
                                                   ccAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGATTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTg                                                                                                 <  2:3835920/150‑1 (MQ=255)
                                                     aaaGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTc                                                                                               >  2:4693940/1‑150 (MQ=255)
                                                      aaGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCa                                                                                              >  2:4061068/1‑150 (MQ=255)
                                                           cAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCaaa                                                                                         >  2:3645228/1‑150 (MQ=255)
                                                             gCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACt                                                                                       <  2:2673495/150‑1 (MQ=255)
                                                             gCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACt                                                                                       <  2:2871223/150‑1 (MQ=255)
                                                               gTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTcc                                                                                     <  1:3820824/150‑1 (MQ=255)
                                                               gTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTcc                                                                                     <  1:2082520/150‑1 (MQ=255)
                                                               gTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTcc                                                                                     <  1:4094617/150‑1 (MQ=255)
                                                                tCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCg                                                                                    <  1:3419583/150‑1 (MQ=255)
                                                                tCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCg                                                                                    <  1:1019553/150‑1 (MQ=255)
                                                                tCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCg                                                                                    <  2:3927953/150‑1 (MQ=255)
                                                                tCCAGGCCAACATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCg                                                                                    <  2:295868/150‑1 (MQ=255)
                                                                  cAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCggg                                                                                  >  2:3401207/1‑150 (MQ=255)
                                                                  cAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCggg                                                                                  >  2:4153862/1‑150 (MQ=255)
                                                                   aGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGt                                                                                 >  2:4331230/1‑150 (MQ=255)
                                                                    ggCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGtt                                                                                <  1:4784300/150‑1 (MQ=255)
                                                                     gCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTa                                                                               <  1:1483274/150‑1 (MQ=255)
                                                                      ccaccaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTAc                                                                              <  1:326796/150‑1 (MQ=255)
                                                                         ccaTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACggg                                                                           <  1:379835/150‑1 (MQ=255)
                                                                          caTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGc                                                                          >  1:2047199/1‑150 (MQ=255)
                                                                           aTACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCg                                                                         <  2:2708650/150‑1 (MQ=255)
                                                                            tACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGa                                                                        <  1:4423848/150‑1 (MQ=255)
                                                                               tcatcaGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAAt                                                                     >  1:4710949/1‑150 (MQ=255)
                                                                                  tcaGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCAt                                                                  >  1:1107483/1‑150 (MQ=255)
                                                                                     gCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTa                                                               <  2:3503208/150‑1 (MQ=255)
                                                                                       aTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAgg                                                             <  1:793975/150‑1 (MQ=255)
                                                                                           cGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACg                                                         <  1:2376322/150‑1 (MQ=255)
                                                                                           cGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACg                                                         <  2:7134/150‑1 (MQ=255)
                                                                                            ggTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACgg                                                        <  2:1091447/150‑1 (MQ=255)
                                                                                            ggTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACgg                                                        >  1:1591401/1‑150 (MQ=255)
                                                                                             gTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGa                                                       <  2:282153/150‑1 (MQ=255)
                                                                                              ttttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAt                                                      >  1:4392526/1‑150 (MQ=255)
                                                                                               tttAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGAtt                                                     >  1:2900346/1‑150 (MQ=255)
                                                                                                   aTGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCa                                                 <  2:760534/150‑1 (MQ=255)
                                                                                                    tGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCaa                                                >  1:4248643/1‑150 (MQ=255)
                                                                                                      ccAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCcaaa                                              >  2:2369369/1‑150 (MQ=255)
                                                                                                       cAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCaaaaa                                             >  1:2595294/1‑150 (MQ=255)
                                                                                                        aaaTCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAAc                                            >  2:1617660/1‑150 (MQ=255)
                                                                                                        aaaTCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAAc                                            >  1:1519106/1‑150 (MQ=255)
                                                                                                         aaTCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACg                                           >  2:1396958/1‑150 (MQ=255)
                                                                                                          aTCGCTTTAAAAAGAATGTGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAAATTTCAGCAAACTCCGGGTTACGGGCGAAATCATGAAGGAACGGATTACCAAAAACGt                                          >  1:1071845/1‑150 (MQ=255)
                                                                                                          aTCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGt                                          >  2:67698/1‑150 (MQ=255)
                                                                                                              cTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTATGAACGGTTTACCAAAAACGTTGGt                                      <  2:4538000/150‑1 (MQ=255)
                                                                                                              cTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGt                                      <  1:1861218/150‑1 (MQ=255)
                                                                                                              cTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGt                                      <  1:3557601/150‑1 (MQ=255)
                                                                                                                  aaaaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAAtt                                  >  2:401475/1‑150 (MQ=255)
                                                                                                                   aaaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAAttt                                 >  1:648334/1‑150 (MQ=255)
                                                                                                                   aaaaGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAAttt                                 >  1:1307740/1‑150 (MQ=255)
                                                                                                                         aTGGGATAGTCAGTATAAAGCCCACTACTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTa                           <  2:1884738/150‑1 (MQ=255)
                                                                                                                          tGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTAc                          >  1:1865982/1‑150 (MQ=255)
                                                                                                                           gggATTGTCAGTATACCGCCCACGTCTGCCATCTGTGAAACTGACAGTATAATGGAGGGATATTTCACCACAAACCTGTCAGCAAACTCCGGGTTACGGGCGATATGATGTAGGAACGGATTACCAAAATCGTTGGTAATTTGCAGTAcc                         <  2:332893/150‑1 (MQ=255)
                                                                                                                            ggATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCCAAAACGTTGGTAAGTTGCAGTACCg                        >  2:4246397/1‑150 (MQ=255)
                                                                                                                               tAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCAc                     >  1:4481081/1‑150 (MQ=255)
                                                                                                                               tAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCAc                     >  1:2831957/1‑150 (MQ=255)
                                                                                                                                aGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCAcc                    >  2:4413614/1‑150 (MQ=255)
                                                                                                                                  tCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAACCGTTGGTAATTTGCAGTACCGCACCCa                  <  1:3320134/150‑1 (MQ=255)
                                                                                                                                   cAGTATAAAGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAg                 >  1:85421/1‑150 (MQ=255)
                                                                                                                                     gTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGca               >  1:2879350/1‑150 (MQ=255)
                                                                                                                                        taAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatca            >  2:1686928/1‑150 (MQ=255)
                                                                                                                                         aaaGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGcatcat           <  1:4030615/150‑1 (MQ=255)
                                                                                                                                          aaGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATg          >  2:99043/1‑150 (MQ=255)
                                                                                                                                           aGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATgg         <  1:3718573/150‑1 (MQ=255)
                                                                                                                                           aGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATgg         <  1:200710/150‑1 (MQ=255)
                                                                                                                                           aGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATgg         <  1:1308972/150‑1 (MQ=255)
                                                                                                                                           aGCCCACGTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATgg         <  1:1124007/150‑1 (MQ=255)
                                                                                                                                             cccACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCa       <  2:4667202/150‑1 (MQ=255)
                                                                                                                                              ccACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCaa      >  1:1208652/1‑150 (MQ=255)
                                                                                                                                                 cTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAaga   <  2:1507941/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAG  >  CP009273/2516407‑2516702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑