Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,619,563 C→A 8.1% R364R (CGA→AGA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,619,5630CA 8.1% 1466.2 / 63.9 391R364R (CGA→AGA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base C (144/215);  new base A (18/14);  total (162/229)
Fisher's exact test for biased strand distribution p-value = 9.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 395 total aligned reads displayed.

CGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGT  >  CP009273/2619416‑2619712
                                                                                                                                                   |                                                                                                                                                     
  tAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATg                                                                                                                                                   <  1:137504/150‑1 (MQ=255)
   aGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATgg                                                                                                                                                  <  2:2580371/150‑1 (MQ=255)
   aGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATgg                                                                                                                                                  <  2:3809898/150‑1 (MQ=255)
    gTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATggg                                                                                                                                                 >  1:1685018/1‑150 (MQ=255)
        cACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGgccgc                                                                                                                                             >  1:41754/1‑150 (MQ=255)
           cGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAt                                                                                                                                          <  1:970868/150‑1 (MQ=255)
            gCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAtg                                                                                                                                         >  2:4332450/1‑150 (MQ=255)
            gCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAtg                                                                                                                                         >  2:62256/1‑150 (MQ=255)
            gCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCAtg                                                                                                                                         >  2:4273417/1‑150 (MQ=255)
              cagcagCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAtgct                                                                                                                                       >  1:2951784/1‑150 (MQ=255)
              cagcagCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCAtgct                                                                                                                                       >  1:673309/1‑150 (MQ=255)
                  agcCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCAt                                                                                                                                   <  1:3281583/150‑1 (MQ=255)
                     cTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAg                                                                                                                                <  2:330497/150‑1 (MQ=255)
                     cTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAg                                                                                                                                <  2:3253859/150‑1 (MQ=255)
                       cGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGt                                                                                                                              <  1:2727259/150‑1 (MQ=255)
                         ccAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCg                                                                                                                            >  2:535983/1‑150 (MQ=255)
                            aCCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGt                                                                                                                         >  1:234849/1‑150 (MQ=255)
                              cAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTg                                                                                                                       >  1:925861/1‑150 (MQ=255)
                                gTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGaa                                                                                                                     <  2:3919485/150‑1 (MQ=255)
                                  aTCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAata                                                                                                                   >  2:1086283/1‑150 (MQ=255)
                                  aTCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAata                                                                                                                   <  1:1491519/150‑1 (MQ=255)
                                    cacaTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATc                                                                                                                 <  2:4669022/150‑1 (MQ=255)
                                    cacaTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATc                                                                                                                 <  2:785025/150‑1 (MQ=255)
                                     acaTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCa                                                                                                                <  1:2614681/150‑1 (MQ=255)
                                      caTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCaa                                                                                                               <  1:1884719/150‑1 (MQ=255)
                                       aTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAAc                                                                                                              >  2:2258525/1‑150 (MQ=255)
                                       aTCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAAc                                                                                                              <  2:3495086/150‑1 (MQ=255)
                                        tCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAAcc                                                                                                             <  2:2319440/150‑1 (MQ=255)
                                         cGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACca                                                                                                            >  1:180444/1‑150 (MQ=255)
                                         cGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACca                                                                                                            <  1:1105753/150‑1 (MQ=255)
                                           aCAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACcaca                                                                                                          >  1:1539567/1‑150 (MQ=255)
                                                gAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGt                                                                                                     >  2:2808955/1‑150 (MQ=255)
                                                   cAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTg                                                                                                  <  1:2055130/150‑1 (MQ=255)
                                                   cAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTg                                                                                                  <  2:3960454/150‑1 (MQ=255)
                                                       cccGACGGGTGCTGGATACCACTATGCAAATGTACGAACTGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATc                                                                                              <  1:4072510/150‑1 (MQ=255)
                                                         cGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGc                                                                                            >  1:514811/1‑150 (MQ=255)
                                                         cGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGc                                                                                            <  2:1314629/150‑1 (MQ=255)
                                                             gggTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACt                                                                                        >  1:2531864/1‑150 (MQ=255)
                                                               gTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTcc                                                                                      <  2:2058272/150‑1 (MQ=255)
                                                               gTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTcc                                                                                      <  1:4246213/150‑1 (MQ=255)
                                                                   tGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCtt                                                                                  <  2:2425627/150‑1 (MQ=255)
                                                                     gATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTtat                                                                                <  1:3248948/150‑1 (MQ=255)
                                                                       tACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTatat                                                                              <  2:4395502/150‑1 (MQ=255)
                                                                        aCCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATAtt                                                                             <  2:3568646/150‑1 (MQ=255)
                                                                        aCCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATAtt                                                                             >  1:4452053/1‑150 (MQ=255)
                                                                         ccACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTc                                                                            <  2:1179059/150‑1 (MQ=255)
                                                                            cTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGc                                                                         >  1:2675202/1‑150 (MQ=255)
                                                                                 cAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAAc                                                                    <  2:2701417/150‑1 (MQ=255)
                                                                                  aaaTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACa                                                                   <  1:467580/150‑1 (MQ=255)
                                                                                  aaaTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACa                                                                   >  2:4051704/1‑150 (MQ=255)
                                                                                  aaaTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACa                                                                   <  1:1232321/150‑1 (MQ=255)
                                                                                  aaaTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACa                                                                   <  1:3429152/150‑1 (MQ=255)
                                                                                   aaTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAg                                                                  <  1:482705/150‑1 (MQ=255)
                                                                                    aTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGt                                                                 <  2:710006/150‑1 (MQ=255)
                                                                                    aTGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTTCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGt                                                                 <  2:1798298/150‑1 (MQ=255)
                                                                                      gTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGa                                                               <  1:1988957/150‑1 (MQ=255)
                                                                                       tACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGAc                                                              >  2:1017949/1‑150 (MQ=255)
                                                                                         cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                            <  1:1945254/150‑1 (MQ=255)
                                                                                         cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                            <  1:4346782/150‑1 (MQ=255)
                                                                                         cGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACtt                                                            >  1:2219967/1‑150 (MQ=255)
                                                                                          gAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTg                                                           >  1:1403655/1‑150 (MQ=255)
                                                                                          gAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTg                                                           >  2:2456224/1‑150 (MQ=255)
                                                                                            aCAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGcc                                                         >  1:2466114/1‑150 (MQ=255)
                                                                                            aCAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGcc                                                         >  1:1739841/1‑150 (MQ=255)
                                                                                               gTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCggg                                                      >  1:736230/1‑150 (MQ=255)
                                                                                                 ggCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTTCTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGtt                                                    <  1:1566432/150‑1 (MQ=255)
                                                                                                    gggAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTa                                                 >  2:1712687/1‑150 (MQ=255)
                                                                                                      gAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAAt                                               <  1:3177658/150‑1 (MQ=255)
                                                                                                           gCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGa                                          <  2:1917868/150‑1 (MQ=255)
                                                                                                            cAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGaa                                         >  2:1436865/1‑150 (MQ=255)
                                                                                                             aaCCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAc                                        >  2:1637979/1‑150 (MQ=255)
                                                                                                              aCCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAca                                       <  1:3484601/150‑1 (MQ=255)
                                                                                                                cGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAAcagc                                     >  1:3746089/1‑150 (MQ=255)
                                                                                                                    gCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCt                                 <  2:1228383/150‑1 (MQ=255)
                                                                                                                      tGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtga                               >  2:2769476/1‑150 (MQ=255)
                                                                                                                       ggCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCtgat                              <  2:1539543/150‑1 (MQ=255)
                                                                                                                              ccGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCg                       <  2:1847668/150‑1 (MQ=255)
                                                                                                                              ccGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCg                       >  2:3078585/1‑150 (MQ=255)
                                                                                                                                  aaCTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGacac                   >  2:3179745/1‑150 (MQ=255)
                                                                                                                                  aaCTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGacac                   >  1:4574381/1‑150 (MQ=255)
                                                                                                                                   aCTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACt                  <  2:2141576/150‑1 (MQ=255)
                                                                                                                                   aCTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACt                  <  2:3664963/150‑1 (MQ=255)
                                                                                                                                    cTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTg                 <  1:4705059/150‑1 (MQ=255)
                                                                                                                                    cTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTg                 <  1:389001/150‑1 (MQ=255)
                                                                                                                                      ggAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCGTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGt               >  1:1032257/1‑150 (MQ=255)
                                                                                                                                      ggAGGCGCTACTGAGATGGGCCGCCAAGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGt               >  1:368755/1‑150 (MQ=255)
                                                                                                                                       gAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTg              >  1:595553/1‑150 (MQ=255)
                                                                                                                                       gAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTg              <  2:41754/150‑1 (MQ=255)
                                                                                                                                       gAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTg              >  1:2273386/1‑150 (MQ=255)
                                                                                                                                        aGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTgc             <  1:1300362/150‑1 (MQ=255)
                                                                                                                                        aGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTgc             <  1:1011478/150‑1 (MQ=255)
                                                                                                                                          gcgcTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTgcgc           <  2:941310/150‑1 (MQ=255)
                                                                                                                                           cgcTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCt          >  1:216521/1‑150 (MQ=255)
                                                                                                                                           cgcTACTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCt          <  1:4273417/150‑1 (MQ=255)
                                                                                                                                               aCTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCa      <  1:3984278/150‑1 (MQ=255)
                                                                                                                                               aCTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCa      <  1:3088537/150‑1 (MQ=255)
                                                                                                                                               aCTGAGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGCCACTGGTgcgcgctca      >  2:2943950/1‑145 (MQ=255)
                                                                                                                                                  gCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCg   <  2:162093/150‑1 (MQ=255)
                                                                                                                                                   cGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGt  >  2:3433286/1‑150 (MQ=255)
                                                                                                                                                   cGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGt  <  2:4447494/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGTAGTCGCACCGCCAGCAGCCTCGCCAACCAGTATCACATCGACAGCGAACAGGCCCGACGGGTGCTGGATACCACTATGCAAATGTACGAACAGTGGCGGGAACAGCAACCGAAGCTGGCGCATCCGCAACTGGAGGCGCTACTGCGATGGGCCGCCATGCTGCATGAGGTCGGGTTGAATATCAACCACAGCGGTTTGCATCGCCACTCCGCTTATATTCTGCAAAACAGTGACTTGCCGGGTTTTAATCAGGAACAGCAGCTGATGATGGCGACACTGGTGCGCTATCACCGT  >  CP009273/2619416‑2619712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑