BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,779,067	C→A	94.0%	intergenic (‑697/+54)	ypjC ← / ← ileY	pseudogene/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,779,067	0	C	A	94.0%	746.3 / 31.7	234	intergenic (‑697/+54)	ypjC/ileY	pseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand): ref base C (6/8); new base A (124/96); total (130/104)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 234 total aligned reads displayed.

TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACA  >  CP009273/2778920‑2779213

tAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGc                                                                                                                                                  >  2:2530739/1‑150 (MQ=255)

 aGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGcc                                                                                                                                                 <  1:770494/150‑1 (MQ=255)

  gCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCa                                                                                                                                                >  1:51639/1‑150 (MQ=255)

  gCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCa                                                                                                                                                <  2:3951232/150‑1 (MQ=255)

        aTTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGt                                                                                                                                          >  1:2402755/1‑150 (MQ=255)

           ttttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAcc                                                                                                                                       >  1:41256/1‑150 (MQ=255)

            tttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAccc                                                                                                                                      >  1:3761761/1‑150 (MQ=255)

                cAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTg                                                                                                                                  <  2:4348751/150‑1 (MQ=255)

                  ggTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTa                                                                                                                                >  1:2046383/1‑150 (MQ=255)

                     aCTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTAccc                                                                                                                             <  1:1647145/150‑1 (MQ=255)

                     aCTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTAccc                                                                                                                             <  1:2683760/150‑1 (MQ=255)

                      cTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCt                                                                                                                            >  1:849980/1‑150 (MQ=255)

                      cTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCt                                                                                                                            <  1:4645466/150‑1 (MQ=255)

                       ttttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCtt                                                                                                                           <  2:1488216/150‑1 (MQ=255)

                          tGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAc                                                                                                                        >  2:340960/1‑150 (MQ=255)

                            aaTGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAActc                                                                                                                      >  2:1109575/1‑150 (MQ=255)

                            aaTGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAActc                                                                                                                      >  2:88392/1‑150 (MQ=255)

                                  tACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                                <  2:2317084/150‑1 (MQ=255)

                                  tACTGATGGATAAATACAGTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                                <  2:3796231/150‑1 (MQ=255)

                                   aCTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGa                                                                                                               <  2:134846/150‑1 (MQ=255)

                                    cTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGAt                                                                                                              >  1:4415293/1‑150 (MQ=255)

                                      gATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATAc                                                                                                            <  2:3992637/150‑1 (MQ=255)

                                      gATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATAc                                                                                                            <  1:643168/150‑1 (MQ=255)

                                       aTGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACg                                                                                                           >  2:676338/1‑150 (MQ=255)

                                         ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTa                                                                                                         >  2:4236239/1‑150 (MQ=255)

                                            tAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaat                                                                                                      >  2:4586929/1‑150 (MQ=255)

                                             aaaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                     >  2:4063176/1‑150 (MQ=255)

                                             aaaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                     <  2:1024655/150‑1 (MQ=255)

                                              aaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataa                                                                                                    <  1:189235/150‑1 (MQ=255)

                                               aTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataat                                                                                                   <  2:3000503/150‑1 (MQ=255)

                                               aTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataat                                                                                                   <  2:439843/150‑1 (MQ=255)

                                                tACATTGCAGTGGAGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCATTGAAATGGAGTTAGTCATGAAATATAGACAGACATCGAGTACCCCATGTACCCTTAACTCATCCTGATACTTAAATAATg                                                                                                  >  1:3399354/1‑150 (MQ=255)

                                                 aCATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGa                                                                                                 <  2:1817187/150‑1 (MQ=255)

                                                  cATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAt                                                                                                >  1:675383/1‑150 (MQ=255)

                                                   aTTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAtt                                                                                               >  2:5470/1‑150 (MQ=255)

                                                     tGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtg                                                                                             <  1:2467260/150‑1 (MQ=255)

                                                      gCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtgg                                                                                            <  2:494798/150‑1 (MQ=255)

                                                        aGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggtg                                                                                          <  1:849360/150‑1 (MQ=255)

                                                        aGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggtg                                                                                          <  1:1866374/150‑1 (MQ=255)

                                                           ggCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAGTTGGTGGcc                                                                                       >  2:2422490/1‑150 (MQ=255)

                                                            gCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGccc                                                                                      <  2:1923771/150‑1 (MQ=255)

                                                               tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                   >  2:370329/1‑150 (MQ=255)

                                                               tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                   >  1:1536828/1‑150 (MQ=255)

                                                                gCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGc                                                                                  <  1:1798235/150‑1 (MQ=255)

                                                                     gTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                             >  2:4192805/1‑150 (MQ=255)

                                                                     gTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                             >  2:1591223/1‑150 (MQ=255)

                                                                        ccAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTg                                                                          >  2:4117587/1‑150 (MQ=255)

                                                                         cAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGa                                                                         <  1:3476768/150‑1 (MQ=255)

                                                                          aaaaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGaa                                                                        >  1:1551372/1‑150 (MQ=255)

                                                                          aaaaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGaa                                                                        >  2:2190262/1‑150 (MQ=255)

                                                                          aaaaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGaa                                                                        >  1:1149355/1‑150 (MQ=255)

                                                                          aaaaCACAAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGaa                                                                        >  2:4590360/1‑150 (MQ=255)

                                                                            aaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAcc                                                                      <  2:2961618/150‑1 (MQ=255)

                                                                               acCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGc                                                                   <  2:3811175/150‑1 (MQ=255)

                                                                                 cAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTAATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTTGCCCTTGCTGGACTTGAACAAGCGa                                                                 >  2:305422/1‑150 (MQ=255)

                                                                                      cTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAg                                                            >  1:4677357/1‑150 (MQ=255)

                                                                                      cTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAg                                                            <  2:1418270/150‑1 (MQ=255)

                                                                                       tCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGc                                                           >  1:352828/1‑150 (MQ=255)

                                                                                          ttCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAt                                                        >  2:3756038/1‑150 (MQ=255)

                                                                                             gAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                     >  1:1190202/1‑150 (MQ=255)

                                                                                              aaaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATg                                                    >  2:1572970/1‑150 (MQ=255)

                                                                                                 ccacccacCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGt                                                 >  1:383851/1‑150 (MQ=255)

                                                                                                   acccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCg                                               >  2:700961/1‑150 (MQ=255)

                                                                                                   acccaccGCACTTCTTCCTTGAAATGGCGTTAGTAATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCg                                               <  1:4534443/150‑1 (MQ=255)

                                                                                                      caccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCt                                            >  1:967640/1‑150 (MQ=255)

                                                                                                       accGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTg                                           <  1:3948886/150‑1 (MQ=255)

                                                                                                       accGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTg                                           <  2:3761245/150‑1 (MQ=255)

                                                                                                            aCTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTa                                      >  1:403011/1‑150 (MQ=255)

                                                                                                               tcttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAcc                                   >  2:2058107/1‑150 (MQ=255)

                                                                                                                cttcCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCa                                  >  2:3331015/1‑150 (MQ=255)

                                                                                                                 ttcCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCAc                                 >  2:1849910/1‑150 (MQ=255)

                                                                                                                 ttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCAc                                 >  2:4638188/1‑150 (MQ=255)

                                                                                                                  tcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACt                                <  2:3896180/150‑1 (MQ=255)

                                                                                                                  tcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACt                                >  1:1835937/1‑150 (MQ=255)

                                                                                                                   ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               >  1:1080679/1‑150 (MQ=255)

                                                                                                                   ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               >  2:4750936/1‑150 (MQ=255)

                                                                                                                   ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               <  2:2898251/150‑1 (MQ=255)

                                                                                                                     ttGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAg                             <  2:2081212/150‑1 (MQ=255)

                                                                                                                     ttGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAg                             <  2:294257/150‑1 (MQ=255)

                                                                                                                             gCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAggg                     <  2:797984/150‑1 (MQ=255)

                                                                                                                             gCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAggg                     <  2:2849585/150‑1 (MQ=255)

                                                                                                                               gTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGcc                   <  1:298796/150‑1 (MQ=255)

                                                                                                                               gTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGcc                   >  2:1242917/1‑150 (MQ=255)

                                                                                                                                ttAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCt                  <  1:3098360/150‑1 (MQ=255)

                                                                                                                                 tAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCtt                 <  1:2753636/150‑1 (MQ=255)

                                                                                                                                 tAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCtt                 <  1:950166/150‑1 (MQ=255)

                                                                                                                                   gTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGa               >  2:3180804/1‑150 (MQ=255)

                                                                                                                                    tCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAg              >  2:1991813/1‑150 (MQ=255)

                                                                                                                                    tCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAg              <  2:1674285/150‑1 (MQ=255)

                                                                                                                                     cATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgt             >  2:2243575/1‑150 (MQ=255)

                                                                                                                                       tGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgtgt           <  1:4355380/150‑1 (MQ=255)

                                                                                                                                         aaaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGc         >  1:2591970/1‑150 (MQ=255)

                                                                                                                                          aaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCa        >  1:2099784/1‑150 (MQ=255)

                                                                                                                                          aaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCa        <  2:3272453/150‑1 (MQ=255)

                                                                                                                                            tataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAt      >  2:3765032/1‑150 (MQ=255)

                                                                                                                                             ataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATa     >  2:1547071/1‑150 (MQ=255)

                                                                                                                                             ataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATa     >  2:3114025/1‑150 (MQ=255)

                                                                                                                                              taGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATaa    <  2:3499711/150‑1 (MQ=255)

                                                                                                                                               aGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAAc   <  2:879007/150‑1 (MQ=255)

                                                                                                                                                gACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACa  <  2:187503/150‑1 (MQ=255)

TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACA  >  CP009273/2778920‑2779213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`