BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	233,931	G→A	33.8%	S37N (AGT→AAT)	yafT →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	233,931	0	G	A	33.8%	223.6 / 182.8	185	S37N (AGT→AAT)	yafT	lipoprotein
Reads supporting (aligned to +/- strand): ref base G (73/43); new base A (33/28); total (106/79)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 191 total aligned reads displayed.

AATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTC  >  CP009273/233782‑234080

aaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAg                                                                                                                                                        >  1:741524/1‑150 (MQ=255)

aaTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAg                                                                                                                                                        >  1:3128625/1‑150 (MQ=255)

  tatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTa                                                                                                                                                      >  2:2555736/1‑150 (MQ=255)

  tatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATa                                                                                                                                                      <  1:4560738/150‑1 (MQ=255)

     tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAata                                                                                                                                                   >  1:4434029/1‑150 (MQ=255)

      cTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAatat                                                                                                                                                  <  1:3563005/150‑1 (MQ=255)

      cTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAatat                                                                                                                                                  >  1:240417/1‑150 (MQ=255)

       tAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATAtt                                                                                                                                                 <  1:1776528/150‑1 (MQ=255)

         aTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaa                                                                                                                                               >  1:2593639/1‑150 (MQ=255)

         aTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATtaa                                                                                                                                               >  2:3677163/1‑150 (MQ=255)

           cATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaata                                                                                                                                             <  2:276816/150‑1 (MQ=255)

           cATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaata                                                                                                                                             >  1:2738300/1‑150 (MQ=255)

           cATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATtaata                                                                                                                                             <  2:2210409/150‑1 (MQ=255)

             ttATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATaaa                                                                                                                                           >  2:539490/1‑150 (MQ=255)

             ttATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATaaa                                                                                                                                           >  2:538675/1‑150 (MQ=255)

              tATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATaaaa                                                                                                                                          <  2:1609175/150‑1 (MQ=255)

              tATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATaaaa                                                                                                                                          >  2:4766718/1‑150 (MQ=255)

                  aCAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAacac                                                                                                                                      <  2:463096/150‑1 (MQ=255)

                        gAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCTAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑a                                                                                                                               <  1:3361003/150‑1 (MQ=255)

                         aGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑Ag                                                                                                                              >  1:3301289/1‑150 (MQ=255)

                         aGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑Ag                                                                                                                              >  1:910262/1‑150 (MQ=255)

                          gggAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGc                                                                                                                             >  1:1258259/1‑150 (MQ=255)

                            gAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCaa                                                                                                                           >  2:3878548/1‑150 (MQ=255)

                             aGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAAc                                                                                                                          >  1:4500601/1‑150 (MQ=255)

                                 tCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTgac                                                                                                                      >  2:473015/1‑150 (MQ=255)

                                  ccAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTgaca                                                                                                                     >  1:3243049/1‑150 (MQ=255)

                                            aTTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGAtt                                                                                                           <  2:4763731/150‑1 (MQ=255)

                                                 aaaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAAGAAACACACATACAAACATTTAta                                                                                                       >  2:1822477/1‑149 (MQ=255)

                                                 aaaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTc                                                                                                      >  2:4223900/1‑150 (MQ=255)

                                                  aaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCg                                                                                                     >  2:2626097/1‑150 (MQ=255)

                                                  aaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCg                                                                                                     <  1:2953028/150‑1 (MQ=255)

                                                   aaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCgg                                                                                                    >  1:795761/1‑150 (MQ=255)

                                                    aaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCAC‑AGCAACTGACAGACAAAGATTTATTCGGt                                                                                                   >  1:1155362/1‑150 (MQ=255)

                                                      gCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTaa                                                                                                 <  1:3336569/150‑1 (MQ=255)

                                                      gCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTaa                                                                                                 <  1:1376264/150‑1 (MQ=255)

                                                        tttGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAATATTTATTCGGTAATg                                                                                               >  1:3706412/1‑150 (MQ=255)

                                                        tttGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATg                                                                                               >  1:1681494/1‑150 (MQ=255)

                                                        tttGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATg                                                                                               <  1:4663117/150‑1 (MQ=255)

                                                          tgttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGaa                                                                                             >  2:3385638/1‑150 (MQ=255)

                                                            ttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAAc                                                                                           <  2:88740/150‑1 (MQ=255)

                                                                atatGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCAct                                                                                       >  1:2910306/1‑150 (MQ=255)

                                                                    gtgtgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTgg                                                                                   >  1:4134621/1‑150 (MQ=255)

                                                                      gtgtTTTTCTCGGTGGTTGATGTTTGTGCCTCTTAATCTTCTTTTTATTAAAAGAATAAAAAAGCTAAAGTCAAAAAAATTAATATTAATAAAAACACTCCCC‑AGAAACTGACAGAAAAATTTTTTTTCGTTAATGAAACCACTCTGGcc                                                                                 <  1:2555736/150‑1 (MQ=255)

                                                                      gtgtTATTCTCGCTGCTTGCAGGATGTGCCTCTTAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGcc                                                                                 <  2:3427040/150‑1 (MQ=255)

                                                                      gtgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGcc                                                                                 <  2:784406/150‑1 (MQ=255)

                                                                        gtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGt                                                                               >  1:4028896/1‑150 (MQ=255)

                                                                        gtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGt                                                                               >  2:3897142/1‑150 (MQ=255)

                                                                           aTTCTCGCTGCTTGCAGTATGTGCCTCTTAATATTATATTGATGAAAATAATAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATc                                                                            <  2:3301289/150‑1 (MQ=255)

                                                                           aTTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATc                                                                            <  2:910262/150‑1 (MQ=255)

                                                                           aTTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATc                                                                            >  1:1943420/1‑150 (MQ=255)

                                                                            ttCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATcc                                                                           <  1:1930347/150‑1 (MQ=255)

                                                                            ttCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATcc                                                                           <  1:1930904/150‑1 (MQ=255)

                                                                             tctcGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCg                                                                          >  1:617662/1‑150 (MQ=255)

                                                                              ctcGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGa                                                                         >  1:503915/1‑150 (MQ=255)

                                                                                cGCTGCTTGCAGGATGTGCCTCTTAATCTTCTATTGATGAAAATAATAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAag                                                                       <  2:1214995/150‑1 (MQ=255)

                                                                                 gctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAaga                                                                      >  2:3033564/1‑150 (MQ=255)

                                                                                 gctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAaga                                                                      >  2:1175707/1‑150 (MQ=255)

                                                                                    gcgtGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAATAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGa                                                                   <  2:1669224/147‑1 (MQ=255)

                                                                                     ctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGat                                                                  >  2:625059/1‑150 (MQ=255)

                                                                                         cAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTc                                                              >  2:26949/1‑150 (MQ=255)

                                                                                          aGGTTGTGCCTATTAATCTTCTATTTATGAAATGATGAAAAACGCTCACTCCAAAAAAAATAATTTTAATAAAAACAGTCCCC‑AGCAAATGACATACAACGTTTTATAGGTTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCa                                                             <  1:4392665/150‑1 (MQ=255)

                                                                                          aGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCa                                                             >  2:2264316/1‑150 (MQ=255)

                                                                                                    tctGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCtt                                                   >  2:3009394/1‑150 (MQ=255)

                                                                                                    tctGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCtt                                                   >  1:1639732/1‑150 (MQ=255)

                                                                                                    tctGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCtt                                                   >  2:1858191/1‑150 (MQ=255)

                                                                                                        aaTTTTCTATTGATGAAAAGAATAAAAAAGCTCAAGTAACAAAAATTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATg                                               <  2:4006011/150‑1 (MQ=255)

                                                                                                        aaTCTTCTATTGATTAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATg                                               <  1:2105695/150‑1 (MQ=255)

                                                                                                        aaTATTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATAATAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATg                                               <  1:3958960/150‑1 (MQ=255)

                                                                                                         atgttctATTTATGAAAAGAAGAAAAAAGCTCATGTAACAAAAAATTATATTAATAAAAACAATCCCC‑ACAAACTGACAGACAAATTTTTATTCTGTATTGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATgg                                              <  1:2368815/147‑1 (MQ=255)

                                                                                                           cttctATTGATTAAAAGAAGAAAAAAGCTCAAGTCACACAAATTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgag                                            <  1:2997298/150‑1 (MQ=255)

                                                                                                           cttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTCATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgag                                            >  1:906427/1‑150 (MQ=255)

                                                                                                           cttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgag                                            >  1:4236035/1‑150 (MQ=255)

                                                                                                           cttatATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAATTAATATTAATAAAAACACGCCCC‑AGAAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgag                                            <  1:2497495/150‑1 (MQ=255)

                                                                                                             tctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGAt                                          >  2:1568964/1‑150 (MQ=255)

                                                                                                              ctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATg                                         >  2:1478394/1‑150 (MQ=255)

                                                                                                                   gatgaAAATAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTc                                    <  2:2500658/150‑1 (MQ=255)

                                                                                                                    atgaAAAGAAGAAAAAAGCTCAAGACACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTcc                                   <  1:2201077/150‑1 (MQ=255)

                                                                                                                       aaaaGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCcgcg                                >  2:4061963/1‑150 (MQ=255)

                                                                                                                        aaaGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGt                               >  2:1374748/1‑150 (MQ=255)

                                                                                                                           caacaaaaaaGCACAAGACACACAAAGTAATAATAATAAAAACAATCCCC‑AGCAACTGACATACAAATATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTcc                            <  1:2513306/146‑1 (MQ=255)

                                                                                                                                aaaaaGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTa                       >  1:1755246/1‑150 (MQ=255)

                                                                                                                                aaaaaGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTa                       <  2:2104687/150‑1 (MQ=255)

                                                                                                                                aaaaaGCTCAAGTCACAAAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTa                       <  2:2104805/150‑1 (MQ=255)

                                                                                                                                  aaaGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAAt                     <  1:2626097/150‑1 (MQ=255)

                                                                                                                                     gCTCAAGTCACAAAAATTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATtct                  <  1:2671900/150‑1 (MQ=255)

                                                                                                                                      cTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATtctc                 >  1:2822910/1‑150 (MQ=255)

                                                                                                                                      cTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATtctc                 >  1:974056/1‑150 (MQ=255)

                                                                                                                                       tCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTcc                <  1:258828/150‑1 (MQ=255)

                                                                                                                                       tCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTcc                >  2:1747284/1‑150 (MQ=255)

                                                                                                                                       tCAAGTCACACAAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTcc                >  2:4533843/1‑150 (MQ=255)

                                                                                                                                        cAAGTAACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGAAAAAGATTTATTCGGTAATTAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCt               <  2:2738300/150‑1 (MQ=255)

                                                                                                                                            tCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTaa           <  1:1136774/150‑1 (MQ=255)

                                                                                                                                            tCACACAAAATAATGTTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTaa           >  1:231532/1‑150 (MQ=255)

                                                                                                                                               cacaAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGTTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATAc        <  1:2436227/150‑1 (MQ=255)

                                                                                                                                               cacaAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATAc        <  2:1502894/150‑1 (MQ=255)

                                                                                                                                                acaAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACt       >  2:4396486/1‑150 (MQ=255)

                                                                                                                                                acaAAATAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACt       >  1:172752/1‑150 (MQ=255)

                                                                                                                                                 caAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACtt      <  1:3919288/150‑1 (MQ=255)

                                                                                                                                                   aaGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAATATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGt    <  1:1056823/150‑1 (MQ=255)

                                                                                                                                                     gTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTc  <  2:2910306/150‑1 (MQ=255)

AATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCC‑AGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTC  >  CP009273/233782‑234080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`