Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,934,843 G→T 93.2% H50N (CAT→AAT)  ygdD ← UPF0382 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,934,8430GT93.2% 841.8 / 48.1 273H50N (CAT→AAT) ygdDUPF0382 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (8/11);  new base T (133/120);  total (141/132)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 274 total aligned reads displayed.

CCACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGG  >  CP009273/2934697‑2934992
                                                                                                                                                  |                                                                                                                                                     
ccACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTaaa                                                                                                                                                    <  2:1370168/150‑1 (MQ=255)
 cacaAACGCTGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCTAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAAc                                                                                                                                                   <  1:1183370/150‑1 (MQ=255)
 cacaAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAAc                                                                                                                                                   >  1:3628463/1‑150 (MQ=255)
   caAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGc                                                                                                                                                 <  1:270170/150‑1 (MQ=255)
     aaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCt                                                                                                                                               <  1:109489/150‑1 (MQ=255)
         cAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTa                                                                                                                                           >  1:1427371/1‑150 (MQ=255)
            aTGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTc                                                                                                                                        >  2:1194083/1‑150 (MQ=255)
            aTGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTc                                                                                                                                        <  2:800018/150‑1 (MQ=255)
            aTGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTc                                                                                                                                        >  2:1731044/1‑150 (MQ=255)
             tGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCg                                                                                                                                       >  2:3305262/1‑150 (MQ=255)
                gACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAgg                                                                                                                                    <  1:2914580/150‑1 (MQ=255)
                 aCAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGc                                                                                                                                   >  2:3012049/1‑150 (MQ=255)
                  cAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGcc                                                                                                                                  >  2:255358/1‑150 (MQ=255)
                    gcgcCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCgg                                                                                                                                <  1:1542653/150‑1 (MQ=255)
                     cgcCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGt                                                                                                                               >  2:2674186/1‑150 (MQ=255)
                       ccAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCt                                                                                                                             >  1:4088487/1‑150 (MQ=255)
                        cAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTg                                                                                                                            <  2:3188136/150‑1 (MQ=255)
                        cAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTg                                                                                                                            <  2:1324461/150‑1 (MQ=255)
                        cAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTg                                                                                                                            >  1:4400322/1‑150 (MQ=255)
                          gccAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGa                                                                                                                          >  2:3424978/3‑150 (MQ=255)
                            cAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATc                                                                                                                        >  2:674257/1‑150 (MQ=255)
                             aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                       <  2:3362588/150‑1 (MQ=255)
                             aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                       >  2:4129540/1‑150 (MQ=255)
                                aaaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGc                                                                                                                    >  2:3847007/1‑150 (MQ=255)
                                aaaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGc                                                                                                                    >  1:1132473/1‑150 (MQ=255)
                                 aaGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGcc                                                                                                                   >  1:188481/1‑150 (MQ=255)
                                  aGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGccc                                                                                                                  <  2:2570012/150‑1 (MQ=255)
                                   ggCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCa                                                                                                                 <  2:1945679/150‑1 (MQ=255)
                                      tGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAACCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                              <  1:2557249/150‑1 (MQ=255)
                                      tGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                              >  1:2512827/1‑150 (MQ=255)
                                        ccGCTGAACAACACCGTGCCTAACTCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCa                                                                                                            <  2:2885028/150‑1 (MQ=255)
                                        ccGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCa                                                                                                            >  1:4695994/1‑150 (MQ=255)
                                          gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAAc                                                                                                          <  2:3727883/150‑1 (MQ=255)
                                          gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAAc                                                                                                          <  2:1186972/150‑1 (MQ=255)
                                          gCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAAc                                                                                                          >  1:841539/1‑150 (MQ=255)
                                            tGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACgg                                                                                                        >  1:3504149/1‑150 (MQ=255)
                                             gAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGc                                                                                                       <  2:1314936/150‑1 (MQ=255)
                                              aacaacACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcc                                                                                                      >  2:3697262/1‑150 (MQ=255)
                                                caacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcccc                                                                                                    >  1:4422049/1‑150 (MQ=255)
                                                 aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAATCGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                   >  2:2806486/1‑150 (MQ=255)
                                                 aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                   >  2:2162099/1‑150 (MQ=255)
                                                 aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                   <  2:1430527/150‑1 (MQ=255)
                                                 aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                   >  1:3774321/1‑150 (MQ=255)
                                                  acacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCa                                                                                                  <  1:770411/150‑1 (MQ=255)
                                                  acacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCa                                                                                                  >  1:3398887/1‑150 (MQ=255)
                                                    acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                                >  1:2492875/1‑150 (MQ=255)
                                                    acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                                <  2:3663918/150‑1 (MQ=255)
                                                     ccGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATgg                                                                                               >  1:94398/1‑150 (MQ=255)
                                                       gTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGtt                                                                                             <  1:2283357/150‑1 (MQ=255)
                                                         gCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGtttt                                                                                           <  2:1586416/150‑1 (MQ=255)
                                                          ccTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTa                                                                                          >  2:1437665/1‑150 (MQ=255)
                                                           cTACCGCGAGTAAAACGCTACTCCAGTAAACCCCGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTAc                                                                                         <  1:4523350/150‑1 (MQ=255)
                                                            tAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACt                                                                                        >  2:511171/1‑150 (MQ=255)
                                                              aCGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTa                                                                                      <  1:604080/150‑1 (MQ=255)
                                                               cgcgAGTAAAACGCTACTCCATTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCACACATAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTaa                                                                                     <  1:3213740/150‑1 (MQ=255)
                                                                     gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                               >  2:2605657/1‑150 (MQ=255)
                                                                     gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                               >  2:1665223/1‑150 (MQ=255)
                                                                      aaaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgc                                                                              <  1:3070852/150‑1 (MQ=255)
                                                                       aaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcg                                                                             >  2:2746354/1‑150 (MQ=255)
                                                                         aCGCTACTCCAGTAAAACCAGATACTTATGCGACGCTGCATTGCCACCGCCAGACCTACGATCGCCAGCTTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgcg                                                                           <  1:2820066/150‑1 (MQ=255)
                                                                         aCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgcg                                                                           >  2:4727561/1‑150 (MQ=255)
                                                                         aCGATACACCAGTAAAACCATATACTGATGCGACGCTGATTTGCCACCGCCAGACCTAAGATCGCCAGCGTATAAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgcg                                                                           <  1:428807/150‑1 (MQ=255)
                                                                           gCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGcc                                                                         <  1:3373220/150‑1 (MQ=255)
                                                                             tACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCaa                                                                       <  1:654980/150‑1 (MQ=255)
                                                                                  cAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAgcg                                                                  >  2:2286351/1‑150 (MQ=255)
                                                                                    gTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGcc                                                                <  2:3892549/150‑1 (MQ=255)
                                                                                        aaCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCaga                                                            >  1:2351125/1‑150 (MQ=255)
                                                                                         aCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                           >  2:1114060/1‑150 (MQ=255)
                                                                                                  tGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaaaa                                                  >  1:2003251/1‑150 (MQ=255)
                                                                                                  tGATGCGACGCTGCATTGCCACCGCAAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaaaa                                                  <  1:3940362/150‑1 (MQ=255)
                                                                                                   gATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAAt                                                 >  1:3872187/1‑150 (MQ=255)
                                                                                                   gATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCCTGGTTTTACTGAACACATGCGCGCCAAAAGCGCCCAAAGCCACAAAAAt                                                 >  1:1514779/1‑150 (MQ=255)
                                                                                                      gCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGaa                                              <  2:493000/150‑1 (MQ=255)
                                                                                                        gACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGc                                            >  2:1529229/1‑150 (MQ=255)
                                                                                                        gACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTGTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACACAAATGAAGc                                            >  1:166264/1‑150 (MQ=255)
                                                                                                        gACGCTGAATTGCCACCGCCATACCTAAGATCGCCAGCTTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGc                                            <  1:580474/150‑1 (MQ=255)
                                                                                                            cTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCt                                        >  1:2056599/1‑150 (MQ=255)
                                                                                                                aTTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAACCGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATg                                    <  2:408657/150‑1 (MQ=255)
                                                                                                                aTTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATg                                    >  2:1013857/1‑150 (MQ=255)
                                                                                                                   gCCACCTCATGACCTACTATCGCCAGGTTTTTAAACGCGTGTTTTTCGAGGCCGGTGGGGACCCTGCCCATAACAAGGGCCCCCATGTTTTTACTTAACACATGCGCGCCAAACGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcg                                 <  1:34832/150‑1 (MQ=255)
                                                                                                                   gCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcg                                 <  2:1612907/150‑1 (MQ=255)
                                                                                                                    ccACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcgg                                <  2:3194500/150‑1 (MQ=255)
                                                                                                                     cACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcggc                               <  1:4315469/150‑1 (MQ=255)
                                                                                                                      aCCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGgcggcg                              >  2:1895223/1‑150 (MQ=255)
                                                                                                                       ccGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGa                             >  1:2086642/1‑150 (MQ=255)
                                                                                                                            aGACCTAAGCTCGCCAGCTTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATc                        <  1:4059329/150‑1 (MQ=255)
                                                                                                                                  aaGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATa                  <  2:3451235/150‑1 (MQ=255)
                                                                                                                                  aaGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATa                  <  2:1469903/150‑1 (MQ=255)
                                                                                                                                  aaGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAACAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATa                  <  1:156452/150‑1 (MQ=255)
                                                                                                                                      tCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAAc              >  1:669693/1‑150 (MQ=255)
                                                                                                                                       cGCCAGCGTATTAACCGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACg             <  2:2988182/150‑1 (MQ=255)
                                                                                                                                       cGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACg             <  1:1463181/150‑1 (MQ=255)
                                                                                                                                          cAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCt          >  2:3675348/1‑150 (MQ=255)
                                                                                                                                            gCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAACGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTgg        <  2:2617285/150‑1 (MQ=255)
                                                                                                                                            gCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTgg        >  1:4168872/1‑150 (MQ=255)
                                                                                                                                            gCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTgg        <  2:4171756/150‑1 (MQ=255)
                                                                                                                                            gCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTgg        >  1:876830/1‑150 (MQ=255)
                                                                                                                                             cGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGt       >  2:1303077/1‑150 (MQ=255)
                                                                                                                                              gTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTc      >  2:245912/1‑150 (MQ=255)
                                                                                                                                              gTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTc      >  2:2359672/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CCACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCATGG  >  CP009273/2934697‑2934992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑