Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,940,935 C→A 5.4% intergenic (+3/‑31) metW → / → metV tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,940,9350CA 5.4% 444.4 / 2.5 152intergenic (+3/‑31)metW/metVtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base C (93/44);  new base A (4/7);  total (97/55)
Fisher's exact test for biased strand distribution p-value = 4.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

CCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTC  >  CP009273/2940786‑2941084
                                                                                                                                                      |                                                                                                                                                     
ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑Tc                                                                                                                                                       <  1:1090439/150‑1 (MQ=255)
ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTGGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑Tc                                                                                                                                                       >  1:4061756/1‑150 (MQ=255)
ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAGTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑Tc                                                                                                                                                       <  1:2986056/150‑1 (MQ=255)
 cGAAGGTCGTCTGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCa                                                                                                                                                      <  2:3675434/150‑1 (MQ=255)
  gAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCaa                                                                                                                                                     >  1:3806944/1‑150 (MQ=255)
   aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAGGAAGTAAATCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGACGGTCGGCGGTTCAAGTCCGGCCACCGCAACCAA‑TCaaa                                                                                                                                                    >  2:3981884/1‑150 (MQ=14)
    aGGTCGTCTGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTCAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAAt                                                                                                                                                   <  2:1128984/150‑1 (MQ=255)
    aGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAAt                                                                                                                                                   >  2:1663/1‑150 (MQ=255)
     ggTCGTCTGTTCAAATCAGGCCCCCGCAACCAATTAAAATTTGTTGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAAtt                                                                                                                                                  <  1:2007363/150‑1 (MQ=255)
        cgtcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAAtaaga                                                                                                                                               >  2:4120353/1‑146 (MQ=255)
          tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTGCACATCCGGCCCCCGCAACCAA‑TCAAATGtgatg                                                                                                                                             >  1:4089733/1‑150 (MQ=255)
             gTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAg                                                                                                                                          <  1:1033261/150‑1 (MQ=255)
               tCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAAGTTGACGAAGtc                                                                                                                                        >  2:2686422/1‑149 (MQ=255)
                 aaaTCCGGCCCCCGCAACCAATTAAAATTGGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAGGAAGTaaa                                                                                                                                      >  1:2130822/1‑150 (MQ=255)
                     ccGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCa                                                                                                                                  <  1:3965826/150‑1 (MQ=255)
                      cGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAg                                                                                                                                 >  1:285580/1‑150 (MQ=255)
                             cGCAACCAATTAAAATTTGATGTAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTg                                                                                                                          <  1:2724914/150‑1 (MQ=255)
                              gCAACCAATTATACATTGATGAAGTAAAGCATTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGa                                                                                                                         <  1:1558392/150‑1 (MQ=255)
                              gCAACCAATTAAAATTTGATGAAGTATAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGa                                                                                                                         <  1:1252430/150‑1 (MQ=255)
                               catccAATTATAATTTGATGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGAc                                                                                                                        <  1:3037252/147‑1 (MQ=255)
                                   cAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGTTGACGAgg                                                                                                                    >  2:65847/1‑150 (MQ=255)
                                    aaTTACAATTTGTTGTATTTTTGCACTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGATCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCggg                                                                                                                   <  2:2923945/150‑1 (MQ=14)
                                    aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCggg                                                                                                                   >  1:3506601/1‑150 (MQ=255)
                                     aTTATATTTTGATGAAGTTATGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgggg                                                                                                                  <  1:653981/150‑1 (MQ=17)
                                      ttAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGAGGGGt                                                                                                                 >  2:471026/1‑150 (MQ=255)
                                        aaaaTTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATATGATGAAGTAAAAGCAGTACGGTGACGCGGGGTgg                                                                                                               >  1:3674979/1‑150 (MQ=255)
                                               gatgaAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGCAGTAATAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                        >  1:779342/1‑150 (MQ=255)
                                                      tAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGc                                                                                                 >  2:3430808/1‑150 (MQ=255)
                                                      tAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTAGGGCTCATAACCCGAAGGTCTTCGGTTCAAATCCGGCCCCCGCAACCAC‑TCAATTATGATGCAGTAATATCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTCGc                                                                                                 >  1:4132137/1‑150 (MQ=17)
                                                       aaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt                                                                                                >  1:1872078/1‑150 (MQ=255)
                                                        aaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtc                                                                                               >  1:4125079/1‑150 (MQ=255)
                                                        aaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtc                                                                                               <  1:3181650/150‑1 (MQ=255)
                                                           gcttccGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                                                                            <  2:1323690/145‑1 (MQ=255)
                                                           cAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATCAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                                                                            >  2:3370985/1‑150 (MQ=255)
                                                            aGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAAGAAGTAAAAGCAGTACTGTGAAGAGGGGTCGAGAAGAATGGAAGCtcgacg                                                                                           >  2:3369442/1‑150 (MQ=14)
                                                            aGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTTGAACAAGTAAATTCATTACTTTGAAGAGGGGTGGAGCAGCCTCGAAGCtctacg                                                                                           >  1:2017694/1‑146 (MQ=14)
                                                              tACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                         <  2:273765/150‑1 (MQ=255)
                                                                cGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCTGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCt                                                                                       >  2:375528/1‑150 (MQ=255)
                                                                cGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGGTCAAATCCGGCCCCCGCAACCAA‑TCAAAGTGGATGAAGTCAGAGCAGTACGGGGACGCGGGGTGGAGCAGCCTGGTCGCTCGTCGGGCt                                                                                       >  2:4302476/1‑150 (MQ=14)
                                                                  gTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGTATGTCGTCGGTTCAAATGCGGCCCCCGCAACCAA‑ACACATTTGATGAAGTAAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                     <  1:1669647/150‑1 (MQ=14)
                                                                   tGACTCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCAGCAACCAA‑TCAAATATGATGAATTAAAAGAAGTACGGTTACGAGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                                                    >  1:4229157/1‑150 (MQ=14)
                                                                   tGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                                                    >  1:2852333/1‑150 (MQ=255)
                                                                        cgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATATGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAccc                                                                               >  2:1566360/1‑150 (MQ=255)
                                                                         ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGTTTTACTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                              <  2:3105425/150‑1 (MQ=255)
                                                                         ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                              <  1:2443520/150‑1 (MQ=40)
                                                                         ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCACCCGCAACCAA‑TCATATTTGTTTCAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                              <  2:3103138/150‑1 (MQ=14)
                                                                           ggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGAAGCTCGACGGGCTCATAACCCGaa                                                                            >  2:4277554/1‑150 (MQ=255)
                                                                            gTGGAGCAGCCTGGTATATCGTCGGTCACATAACCCGAAGGTCGTCGGTTCAAATCCTTACCCCGCAACCAA‑TCAAATTTGATGCACTACAACCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAg                                                                           <  1:1007043/150‑1 (MQ=11)
                                                                             tGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTTGATGCAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAgg                                                                          <  1:2781039/150‑1 (MQ=25)
                                                                               gaccagcCTGTCAGCTCGTCGCGCTCACAACCCTCAGTTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGTTTAACTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                        <  2:4089733/147‑1 (MQ=14)
                                                                               gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCACATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                        <  1:752589/150‑1 (MQ=255)
                                                                                   agcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGtcgtcg                                                                    >  2:445834/1‑150 (MQ=40)
                                                                                      gtggttggtCGTCTGTCTATTATCCCGAAGGTGGTCGGTTCATATCCGTCACCCGCAACCAA‑TCCTATTTGTTGCACTATACGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                 <  1:3930995/149‑1 (MQ=2)
                                                                                      cTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                 <  1:882287/150‑1 (MQ=40)
                                                                                         gTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATTAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaa                                                              <  2:4183004/150‑1 (MQ=255)
                                                                                          tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAAGTTGATGAAGTAAAAGCAGTACGGTGACGAGGGGTGCAGCAGCCTGGTAGCTCGACGGGCGCATAACCCGAAGGACGTCGGTTCtaa                                                             >  1:1910975/1‑150 (MQ=14)
                                                                                            gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACACGAAGGTCGTCGGGTCAAATc                                                           >  2:3275936/1‑150 (MQ=255)
                                                                                              tcgtcgGGCTCATAACCCTAAGGTCGTCGGTTCAAATCCGGACCCCGCAACCAA‑TTATATTTGTTGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg                                                         <  2:1779479/150‑1 (MQ=11)
                                                                                               cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                        >  2:2596266/1‑150 (MQ=40)
                                                                                               cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                        >  1:1267202/1‑150 (MQ=40)
                                                                                                gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGc                                                       >  2:1357019/1‑150 (MQ=40)
                                                                                                  cgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGAAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGACGGGCTCATAACCCGAAGCTCGTCGGTTCAAATCCGGccc                                                     >  1:1419014/1‑150 (MQ=255)
                                                                                                   gggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGcccc                                                    >  1:68797/1‑150 (MQ=40)
                                                                                                         aTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                              >  1:2474242/1‑150 (MQ=40)
                                                                                                          tAACCCGAAGGTCGTCGTTTGTAATGCGGCCCCGGCATCCAT‑TTAAATTTGTTTGTTTATATGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc                                             <  2:2763979/150‑1 (MQ=11)
                                                                                                          tAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAcc                                             >  1:2943019/1‑150 (MQ=40)
                                                                                                           aaCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAAGTTGATGAAGTAAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                            <  1:91606/150‑1 (MQ=16)
                                                                                                                 aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAattatt                                      >  1:1132991/1‑150 (MQ=255)
                                                                                                                   ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAC‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGa                                    >  1:1690555/1‑150 (MQ=255)
                                                                                                                    gtcgtcGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTTCTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTAATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGATTgta                                   >  2:498057/1‑148 (MQ=255)
                                                                                                                    gtcgtcGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGaa                                   >  2:2889848/1‑150 (MQ=255)
                                                                                                                       gtcgGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAacac                                >  1:2523166/1‑150 (MQ=255)
                                                                                                                       gtcgGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAacac                                >  1:2523196/1‑150 (MQ=255)
                                                                                                                          ggTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAATGCAGTACGGTGACCCGGGGTGGAGCATCCTGGTAGCTCGACGGGCTCATAACACGAAGGTCGGCGGTGCAAGTCCGGCCCCCGCAACCAATTATTGTACAccca                             >  2:1007734/1‑149 (MQ=14)
                                                                                                                          ggTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATTATTGAACACCCt                             >  2:3886854/1‑150 (MQ=255)
                                                                                                                             tCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAAc                          >  2:4376169/1‑150 (MQ=255)
                                                                                                                               aaaTCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACgg                        >  1:1313332/1‑150 (MQ=255)
                                                                                                                               aaaTCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACgg                        >  1:4310730/1‑150 (MQ=255)
                                                                                                                                 aTCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGgt                      >  2:3002649/1‑150 (MQ=255)
                                                                                                                                 aTCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGgt                      >  2:1901773/1‑150 (MQ=255)
                                                                                                                                 aTCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTGAACACCCTAACGGgt                      >  2:3000339/1‑150 (MQ=255)
                                                                                                                                     ggCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttt                  <  1:434591/150‑1 (MQ=255)
                                                                                                                                      tcccccGCAACGAA‑ACATAGTTGTTGCACTATTCGCAGTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttt                 <  1:946714/149‑1 (MQ=17)
                                                                                                                                        ccccGCCACCAC‑TCAAATTTGTTGGACTTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttttt               <  2:2767887/150‑1 (MQ=18)
                                                                                                                                         gccGCAACCAA‑TCAAATTTGTTGTAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAGAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt              <  2:3674979/149‑1 (MQ=255)
                                                                                                                                         cccGCAACCAATTAAAATTTTAT‑CACTTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt              <  2:326888/150‑1 (MQ=14)
                                                                                                                                         cccGCAACCAA‑ACAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt              <  1:221476/150‑1 (MQ=255)
                                                                                                                                          ccGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGGGTTTTTTTg             >  1:2598955/1‑150 (MQ=14)
                                                                                                                                           cGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGt            >  1:2114611/1‑150 (MQ=255)
                                                                                                                                            gCAACCAA‑TTAAATTTGTTGGATGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGtt           <  2:1921602/150‑1 (MQ=17)
                                                                                                                                            gCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGtt           >  1:2842526/1‑150 (MQ=255)
                                                                                                                                               aCCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCt        >  2:2867729/1‑150 (MQ=255)
                                                                                                                                                 cAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTgg      >  1:325203/1‑150 (MQ=255)
                                                                                                                                                 cAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTgg      >  1:4773182/1‑150 (MQ=255)
                                                                                                                                                 cAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTgg      >  1:4773220/1‑150 (MQ=255)
                                                                                                                                                 cAA‑TCAAAGTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTgg      >  1:4773766/1‑150 (MQ=255)
                                                                                                                                                  aa‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGt     >  2:3982025/1‑150 (MQ=255)
                                                                                                                                                  aa‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGt     >  1:4521382/1‑150 (MQ=255)
                                                                                                                                                  aa‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGt     >  1:4521404/1‑150 (MQ=255)
                                                                                                                                                      cAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGtctc  >  2:2261173/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTC  >  CP009273/2940786‑2941084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑