Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,092,182 C→A 19.4% C248F (TGT→TTT)  yggM ← DUF1202 family putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,092,1820CA19.4% 591.9 / 101.9 217C248F (TGT→TTT) yggMDUF1202 family putative secreted protein
Reads supporting (aligned to +/- strand):  ref base C (96/79);  new base A (25/17);  total (121/96)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 223 total aligned reads displayed.

ACTTTCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTGC  >  CP009273/3092035‑3092331
                                                                                                                                                   |                                                                                                                                                     
aCTTTCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAaaac                                                                                                                                                     <  2:2851157/150‑1 (MQ=255)
  tttCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACActc                                                                                                                                                   <  2:4011182/150‑1 (MQ=255)
    tCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCtt                                                                                                                                                 <  2:2007974/150‑1 (MQ=255)
     cATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTc                                                                                                                                                >  1:4706367/1‑150 (MQ=255)
      aTCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCa                                                                                                                                               <  1:1522096/150‑1 (MQ=255)
       tCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCAc                                                                                                                                              <  2:3364556/150‑1 (MQ=255)
          ttAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCActct                                                                                                                                           >  1:3660026/1‑150 (MQ=255)
           tAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTg                                                                                                                                          >  2:3312405/1‑150 (MQ=255)
            aaCGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGt                                                                                                                                         >  2:799893/1‑150 (MQ=255)
            aaCGGATATTGCACTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATCACACTCTTCACTCTGt                                                                                                                                         <  1:1289588/150‑1 (MQ=255)
             aCGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGtt                                                                                                                                        >  2:135482/1‑150 (MQ=255)
             aCGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGtt                                                                                                                                        >  2:2400317/1‑150 (MQ=255)
              cGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGttt                                                                                                                                       <  1:2819750/150‑1 (MQ=255)
              cGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGttt                                                                                                                                       >  2:3262719/1‑150 (MQ=255)
              cGGAGATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGttt                                                                                                                                       <  1:4422456/150‑1 (MQ=255)
               ggATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTa                                                                                                                                      >  2:4682682/1‑150 (MQ=255)
                 atatTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATg                                                                                                                                    >  1:389967/1‑150 (MQ=255)
                     tGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACAt                                                                                                                                <  1:527474/150‑1 (MQ=255)
                       cccTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGc                                                                                                                              >  1:4176660/1‑150 (MQ=255)
                        ccTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGcc                                                                                                                             <  2:4616933/150‑1 (MQ=255)
                         cTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTCCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCg                                                                                                                            <  1:2049584/150‑1 (MQ=255)
                           ttttCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCa                                                                                                                          <  1:822044/150‑1 (MQ=255)
                              tCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATc                                                                                                                       <  2:1953350/150‑1 (MQ=255)
                                 aGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGcc                                                                                                                    >  2:1793118/1‑150 (MQ=255)
                                    gTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGt                                                                                                                 >  1:445825/1‑150 (MQ=255)
                                     ttACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGtt                                                                                                                <  2:1186781/150‑1 (MQ=255)
                                        cccAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCCGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGAt                                                                                                             >  1:2572393/1‑150 (MQ=255)
                                          cAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATaa                                                                                                           >  2:3900572/1‑150 (MQ=255)
                                           aGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATaaa                                                                                                          <  2:1758954/150‑1 (MQ=255)
                                            gTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAAc                                                                                                         >  1:3769815/1‑150 (MQ=255)
                                              cATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACct                                                                                                       <  2:1229764/150‑1 (MQ=255)
                                               aTCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACctc                                                                                                      <  1:3211523/150‑1 (MQ=255)
                                                 ccTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCtt                                                                                                    >  1:3790864/1‑150 (MQ=255)
                                                 ccTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCtt                                                                                                    >  1:1382726/1‑150 (MQ=255)
                                                  cTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCttt                                                                                                   >  2:3657465/1‑150 (MQ=255)
                                                  cTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCttt                                                                                                   >  2:1193323/1‑150 (MQ=255)
                                                    gCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGt                                                                                                 >  1:729005/1‑150 (MQ=255)
                                                     cAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTc                                                                                                >  2:3198096/1‑150 (MQ=255)
                                                      aaTTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCa                                                                                               >  1:2285763/1‑150 (MQ=255)
                                                        tttACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCata                                                                                             <  2:2282134/150‑1 (MQ=255)
                                                         ttACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATTACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCatat                                                                                            >  2:2559017/1‑150 (MQ=255)
                                                             ggCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTaa                                                                                        <  1:2545316/150‑1 (MQ=255)
                                                                     ttttGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAAc                                                                                <  1:778863/150‑1 (MQ=255)
                                                                      tttGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACg                                                                               >  1:1044398/1‑150 (MQ=255)
                                                                          tgATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTc                                                                           <  1:1048914/150‑1 (MQ=255)
                                                                            aTTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTAATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGc                                                                         <  1:4006792/150‑1 (MQ=255)
                                                                             ttGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCt                                                                        <  1:4152604/150‑1 (MQ=255)
                                                                               gCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTAt                                                                      <  2:470442/150‑1 (MQ=255)
                                                                                cAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATc                                                                     >  2:1818077/1‑150 (MQ=255)
                                                                                 aGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCg                                                                    >  2:3495144/1‑150 (MQ=255)
                                                                                     aaGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCt                                                                <  1:1175650/150‑1 (MQ=255)
                                                                                      aGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTg                                                               <  2:4195323/150‑1 (MQ=255)
                                                                                      aGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTg                                                               <  2:1864613/150‑1 (MQ=255)
                                                                                              gCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTaa                                                       >  2:4104182/1‑150 (MQ=255)
                                                                                                cGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAAc                                                     <  1:1073820/150‑1 (MQ=255)
                                                                                                 gttgttCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACt                                                    >  2:2132573/1‑150 (MQ=255)
                                                                                                  ttgttCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACtc                                                   >  2:4755533/1‑150 (MQ=255)
                                                                                                     ttCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACtcatc                                                >  1:3226371/1‑150 (MQ=255)
                                                                                                      tCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACtcatca                                               <  2:462775/150‑1 (MQ=255)
                                                                                                       cGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCAc                                              >  2:3505923/1‑150 (MQ=255)
                                                                                                        gTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACg                                             >  2:1478891/1‑150 (MQ=255)
                                                                                                          tGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCt                                           <  2:4251086/150‑1 (MQ=255)
                                                                                                           gATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTg                                          >  1:3515691/1‑150 (MQ=255)
                                                                                                              atCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTg                                       >  1:3924812/1‑150 (MQ=255)
                                                                                                              atCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTg                                       <  2:4348352/150‑1 (MQ=255)
                                                                                                               tCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTgg                                      >  1:4748476/1‑150 (MQ=255)
                                                                                                                 gAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTg                                    >  2:739022/1‑150 (MQ=255)
                                                                                                                  aaaTCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGa                                   >  1:2502653/1‑150 (MQ=255)
                                                                                                                  aaaTCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGa                                   <  1:12686/150‑1 (MQ=255)
                                                                                                                  aaaTCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGa                                   >  1:932215/1‑150 (MQ=255)
                                                                                                                   aaTCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGaa                                  >  1:3038784/1‑150 (MQ=255)
                                                                                                                   aaTCTCGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGaa                                  >  2:359794/1‑150 (MQ=255)
                                                                                                                    aTCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAt                                 >  1:3596387/1‑150 (MQ=255)
                                                                                                                     tctcGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAtt                                <  1:3804879/150‑1 (MQ=255)
                                                                                                                        cGTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAttttt                             >  2:3855631/1‑150 (MQ=255)
                                                                                                                         gTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAtttttt                            <  1:1009764/150‑1 (MQ=255)
                                                                                                                         gTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAtttttt                            >  2:153216/1‑150 (MQ=255)
                                                                                                                         gTTTCTGCTGAATCGGCACTTCATAAAACTCTTCACTCTTTTTATGACATGCCGAAATCGCCGGTTTATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAAtttttt                            <  1:4325/150‑1 (MQ=255)
                                                                                                                            tCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTaa                         <  1:3164627/150‑1 (MQ=255)
                                                                                                                             ctgctgAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTaaa                        <  1:1922028/150‑1 (MQ=255)
                                                                                                                               gctgAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAgg                      <  1:1770515/150‑1 (MQ=255)
                                                                                                                                     tCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCtt                <  1:859171/150‑1 (MQ=255)
                                                                                                                                     tCGGCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCtt                >  2:3742964/1‑150 (MQ=255)
                                                                                                                                      cGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTc               >  1:1393073/1‑150 (MQ=255)
                                                                                                                                      cGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTc               >  1:1680914/1‑150 (MQ=255)
                                                                                                                                      cGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTc               <  2:728885/150‑1 (MQ=255)
                                                                                                                                       ggCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTcg              >  2:269375/1‑150 (MQ=255)
                                                                                                                                       ggCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAAACTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTcg              >  2:3309100/1‑150 (MQ=255)
                                                                                                                                        gCACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTcgc             >  1:4270366/1‑150 (MQ=255)
                                                                                                                                         cACTTCATAAAACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTcgcg            <  2:4706367/150‑1 (MQ=255)
                                                                                                                                             tCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTc        <  1:4742287/150‑1 (MQ=255)
                                                                                                                                              cATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCa       >  1:1449056/1‑150 (MQ=255)
                                                                                                                                              cATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCa       >  2:3037446/1‑150 (MQ=255)
                                                                                                                                              cATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCa       >  2:136859/1‑150 (MQ=255)
                                                                                                                                               aTACCACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAt      <  1:1598536/150‑1 (MQ=255)
                                                                                                                                               aTAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTACCCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAt      <  1:1630812/150‑1 (MQ=255)
                                                                                                                                                tAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCAtt     >  1:42506/1‑150 (MQ=255)
                                                                                                                                                  acacTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTg   <  1:3969887/150‑1 (MQ=255)
                                                                                                                                                  acacTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTg   >  1:491456/1‑150 (MQ=255)
                                                                                                                                                   cacTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTGc  >  1:3449323/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACTTTCATCGTTAACGGATATTGCCCTTTTTCGAGCGTTACCCAGTCATCCTGCAATTTACGGCTAAGTTTTTGTGATTGCAGAAAAGTCTGTCGCCGTTGTTCGTTGATATCGAAATCTCGTTTCTGCTGAATCGGCACTTCATAACACTCTTCACTCTGTTTATGACATGCCGCAATCGCCGGTTGATAAACCTCTTTGTCATATTTAACCGCGAACGCCTCGCTATCGTTCTGTTTATTAAACTCATCACGCTGTTGGTGAATTTTTTTAAAGGCGTCTTCGCGGGTCATTTGC  >  CP009273/3092035‑3092331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑