Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,102,941 T→A 6.5% S10R (AGT→AGA)  yqgA → DUF554 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,102,9410TA 6.5% 1164.8 / 53.2 338S10R (AGT→AGA) yqgADUF554 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (164/152);  new base A (9/13);  total (173/165)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 340 total aligned reads displayed.

GCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCTTCCAGCGATGGT  >  CP009273/3102792‑3103090
                                                                                                                                                     |                                                                                                                                                     
   cAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCa                                                                                                                                                    >  2:2880773/1‑150 (MQ=255)
   cAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCa                                                                                                                                                    >  2:19781/1‑150 (MQ=255)
     aTCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGt                                                                                                                                                  <  1:1880333/150‑1 (MQ=255)
        aTGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCtt                                                                                                                                               >  1:3121385/1‑150 (MQ=255)
          ggCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTAc                                                                                                                                             <  2:2922626/150‑1 (MQ=255)
          ggCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTAc                                                                                                                                             >  2:4745261/1‑150 (MQ=255)
          ggCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTAc                                                                                                                                             >  2:2259560/1‑150 (MQ=255)
              ccTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTggg                                                                                                                                         >  2:3567617/1‑150 (MQ=255)
               cTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGt                                                                                                                                        >  1:3173612/1‑150 (MQ=255)
                tttCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTg                                                                                                                                       <  1:2202555/150‑1 (MQ=255)
                  tCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGc                                                                                                                                     <  1:2641511/150‑1 (MQ=255)
                        aaGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTtctc                                                                                                                               >  2:4261888/1‑150 (MQ=255)
                         aGCGTGCAAGCCAGGACTTTGCGAACAAACACCCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACACTACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCg                                                                                                                              <  1:2803560/150‑1 (MQ=255)
                         aGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCg                                                                                                                              >  1:3053220/1‑150 (MQ=255)
                            gTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCGAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGgcg                                                                                                                           <  2:4006731/150‑1 (MQ=255)
                                aaGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACt                                                                                                                       >  2:2030573/1‑150 (MQ=255)
                                aaGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACt                                                                                                                       <  2:605412/150‑1 (MQ=255)
                                   ccAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCt                                                                                                                    <  2:4650338/150‑1 (MQ=255)
                                     aGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCa                                                                                                                  >  1:1553494/1‑150 (MQ=255)
                                        aCTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGcc                                                                                                               <  2:1646383/150‑1 (MQ=255)
                                         cTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCa                                                                                                              >  1:1722381/1‑150 (MQ=255)
                                          tttGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCaa                                                                                                             <  2:2089643/150‑1 (MQ=255)
                                               gAACAACCAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTACCAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCtt                                                                                                        <  1:1388182/150‑1 (MQ=255)
                                               gAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCtt                                                                                                        >  1:674431/1‑150 (MQ=255)
                                                   aaacaaGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTCGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCg                                                                                                    >  2:3364948/1‑150 (MQ=255)
                                                   aaacaaGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCg                                                                                                    >  1:3921374/1‑150 (MQ=255)
                                                   aaacaaGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCg                                                                                                    >  1:1514788/1‑150 (MQ=255)
                                                   aaacaaGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCg                                                                                                    >  2:3129910/1‑150 (MQ=255)
                                                      caaGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGaa                                                                                                 <  2:3209688/150‑1 (MQ=255)
                                                       aaGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAAc                                                                                                <  2:176808/150‑1 (MQ=255)
                                                           cATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTAt                                                                                            <  1:1293113/150‑1 (MQ=255)
                                                            aTCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATc                                                                                           >  2:1084850/1‑150 (MQ=255)
                                                             tCGACGGCACCAAACGGTCATACTAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGCATcc                                                                                          <  1:1692132/150‑1 (MQ=255)
                                                             tCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATcc                                                                                          >  2:2495616/1‑150 (MQ=255)
                                                             tCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATcc                                                                                          <  1:427061/150‑1 (MQ=255)
                                                               gACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACAGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCcgc                                                                                        >  1:405013/1‑150 (MQ=255)
                                                                  ggCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGtt                                                                                                                                  <  1:2431138/105‑1 (MQ=255)
                                                                   gCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGtct                                                                                    <  1:4376692/150‑1 (MQ=255)
                                                                   gCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGtct                                                                                    <  2:4109722/150‑1 (MQ=255)
                                                                     aCCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTcc                                                                                  >  2:1119033/1‑150 (MQ=255)
                                                                     aCCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTcc                                                                                  <  2:1417225/150‑1 (MQ=255)
                                                                       cAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCAt                                                                                >  2:1344534/1‑150 (MQ=255)
                                                                        aaaCGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATg                                                                               <  1:2094713/150‑1 (MQ=255)
                                                                        aaaCGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATg                                                                               <  1:775328/150‑1 (MQ=255)
                                                                         aaCGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGa                                                                              >  1:2107049/1‑150 (MQ=255)
                                                                         aaCGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGa                                                                              >  1:2050062/1‑150 (MQ=255)
                                                                           cGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACa                                                                            >  2:2457563/1‑150 (MQ=255)
                                                                               cATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCaa                                                                        >  1:4103888/1‑150 (MQ=255)
                                                                                ataataAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAAt                                                                       <  1:4649015/150‑1 (MQ=255)
                                                                                 taataaGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAAtt                                                                      >  2:2426814/1‑150 (MQ=255)
                                                                                   ataaGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAAtttt                                                                    <  2:4534422/150‑1 (MQ=255)
                                                                                     aaGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTg                                                                  <  2:944337/150‑1 (MQ=255)
                                                                                      aGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCGTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTgg                                                                 >  1:3600091/1‑150 (MQ=255)
                                                                                         aaaTCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCt                                                              >  1:4588615/1‑150 (MQ=255)
                                                                                          aaTCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTg                                                             >  2:510169/1‑150 (MQ=255)
                                                                                          aaTCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTg                                                             <  1:3927497/150‑1 (MQ=255)
                                                                                                 cAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGc                                                      <  2:3835303/150‑1 (MQ=255)
                                                                                                  aaTACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCt                                                     >  2:1545887/1‑150 (MQ=255)
                                                                                                   aTACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTg                                                    <  1:28368/150‑1 (MQ=255)
                                                                                                    tACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTgg                                                   <  2:2722672/150‑1 (MQ=255)
                                                                                                      catcTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGATAGTGCAGTCTTACTGGGTTGCGTTCTCGGCGAAATGATCAGCAAAAGCTTACCGGAACGTAAACGCTTATCCATGACATCAATTTTTGGTCTGGCATCGCTgggg                                                 >  2:340566/4‑150 (MQ=255)
                                                                                                      cAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTgggg                                                 >  1:1814231/1‑150 (MQ=255)
                                                                                                       aGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGa                                                >  1:4704150/1‑150 (MQ=255)
                                                                                                         cTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGAtt                                              >  1:478987/1‑150 (MQ=255)
                                                                                                         cTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGAtt                                              >  2:1384473/1‑150 (MQ=255)
                                                                                                          tAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTg                                             >  1:580681/1‑150 (MQ=255)
                                                                                                           aaCAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTgg                                            <  1:302206/150‑1 (MQ=255)
                                                                                                           aaCAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTgg                                            <  1:3325116/150‑1 (MQ=255)
                                                                                                            aCAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGt                                           <  2:3614893/150‑1 (MQ=255)
                                                                                                              acgAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTAt                                         >  2:594328/3‑150 (MQ=255)
                                                                                                              aGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTAt                                         >  2:377879/1‑150 (MQ=255)
                                                                                                              aGGAACTCTTGTGGTCATCGACCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTAt                                         >  1:3644600/1‑150 (MQ=255)
                                                                                                                gAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTAttt                                       <  1:3134113/150‑1 (MQ=255)
                                                                                                                 aaCTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTAtttt                                      <  2:3727000/150‑1 (MQ=255)
                                                                                                                  aCTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACCTATCCGCGTCTCCAAGACATCAATTTTTGGTCTGGCAACGCTGGGGATTGGTATTTTa                                     >  1:443731/1‑150 (MQ=255)
                                                                                                                    tctTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACt                                   <  2:4317090/150‑1 (MQ=255)
                                                                                                                    tctTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACt                                   >  1:1267776/1‑150 (MQ=255)
                                                                                                                    tctTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACt                                   <  1:3800828/150‑1 (MQ=255)
                                                                                                                     ctTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACtg                                  >  2:2794851/1‑150 (MQ=255)
                                                                                                                      ttGTGGTCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACtgg                                 <  1:2259880/150‑1 (MQ=255)
                                                                                                                       tgtgGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACtggt                                <  1:2821424/150‑1 (MQ=255)
                                                                                                                        gtgGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACtggtg                               <  2:3053220/150‑1 (MQ=255)
                                                                                                                          ggTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTggtggt                             <  1:526188/150‑1 (MQ=255)
                                                                                                                            tCATCGGCCCTTTTATCAACGCTAGAGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGa                           <  2:3172918/150‑1 (MQ=255)
                                                                                                                             cATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGaa                          >  2:3267211/1‑150 (MQ=255)
                                                                                                                             cATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGaa                          <  1:2596312/150‑1 (MQ=255)
                                                                                                                             cATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGAGTGGTATTTTACTGGTGGTGaa                          >  2:4422529/1‑150 (MQ=255)
                                                                                                                              aTCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGaaa                         <  2:1234123/150‑1 (MQ=255)
                                                                                                                               tCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAAt                        <  2:768323/150‑1 (MQ=255)
                                                                                                                                 ggCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAAtgt                      >  1:3398072/1‑150 (MQ=255)
                                                                                                                                 ggCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAAtgt                      <  2:2229144/150‑1 (MQ=255)
                                                                                                                                   cccTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGc                    >  1:2700612/1‑150 (MQ=255)
                                                                                                                                       tttATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAAc                >  1:3520736/1‑150 (MQ=255)
                                                                                                                                        ttATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAAcc               >  2:2625122/1‑150 (MQ=255)
                                                                                                                                         tATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCt              >  2:1914172/1‑150 (MQ=255)
                                                                                                                                         tATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCt              >  2:3509419/1‑150 (MQ=255)
                                                                                                                                           tCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCTTc            >  1:2544088/1‑150 (MQ=255)
                                                                                                                                             aaCGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCTTCCa          >  2:4212494/1‑150 (MQ=255)
                                                                                                                                             aaCGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCTTCCa          >  1:4474036/1‑150 (MQ=255)
                                                                                                                                                 cTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCTTCCAGCGa      <  1:1031172/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGGAACTCTTGTGGTCATCGGCCCTTTTATCAACGCTAGTGCAGTCTTACTGGGTGGCGTTCTCGGCGCACTGCTCAGCCAACGCTTACCGGAACGTATCCGCGTCTCCATGACATCAATTTTTGGTCTGGCATCGCTGGGGATTGGTATTTTACTGGTGGTGAAATGTGCCAACCTTCCAGCGATGGT  >  CP009273/3102792‑3103090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑