Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,104,247 C→A 25.8% R80L (CGA→CTA)  yghD ← putative membrane‑anchored secretion pathway M‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,104,2470CA25.8% 708.7 / 216.5 309R80L (CGA→CTA) yghDputative membrane‑anchored secretion pathway M‑type protein
Reads supporting (aligned to +/- strand):  ref base C (134/95);  new base A (37/43);  total (171/138)
Fisher's exact test for biased strand distribution p-value = 6.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

ACCTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAACGCCCC  >  CP009273/3104100‑3104396
                                                                                                                                                   |                                                                                                                                                     
  cTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGgt                                                                                                                                                   >  1:4694196/1‑150 (MQ=255)
    gAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGgtgt                                                                                                                                                 <  2:4107149/150‑1 (MQ=255)
    gAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGAGACCTGCGCAGGCGTTGTTGTGGCCGATTTTCGCGCTGCAATAATCTCCGGCGCGGCGTTCTTCAGTCGGgtgt                                                                                                                                                 >  2:3989051/1‑150 (MQ=255)
    gAATATTCTCCACACGATCGGATATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCTCTGCAATAATATCCCGCTCGGCGTTCTTCAGTCGGgtgt                                                                                                                                                 >  2:740581/1‑150 (MQ=255)
       tatTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCa                                                                                                                                              <  1:3385756/150‑1 (MQ=255)
       tatTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCa                                                                                                                                              <  1:628950/150‑1 (MQ=255)
       tatTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCa                                                                                                                                              <  1:4116261/150‑1 (MQ=255)
         ttCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGACGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATc                                                                                                                                            >  1:1841701/1‑150 (MQ=255)
          tctcCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCg                                                                                                                                           <  2:1104935/150‑1 (MQ=255)
             ccccACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGcca                                                                                                                                        >  2:3961760/1‑150 (MQ=255)
               ccACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGccacc                                                                                                                                      >  2:4744206/1‑150 (MQ=255)
                cACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGAAGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGccacca                                                                                                                                     >  2:785180/1‑150 (MQ=255)
                  cGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgc                                                                                                                                   >  1:3205752/1‑150 (MQ=255)
                   gATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgct                                                                                                                                  >  1:805670/1‑150 (MQ=255)
                   gATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgct                                                                                                                                  >  1:40198/1‑150 (MQ=255)
                    aTCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctg                                                                                                                                 >  1:3132977/1‑150 (MQ=255)
                     tCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctgc                                                                                                                                <  1:4264463/150‑1 (MQ=255)
                      cGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctgct                                                                                                                               >  2:4651935/1‑150 (MQ=255)
                      cGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctgct                                                                                                                               >  1:721855/1‑150 (MQ=255)
                       ggCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGctgctg                                                                                                                              <  1:838128/150‑1 (MQ=255)
                       ggCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGctgctg                                                                                                                              >  2:3591477/1‑150 (MQ=255)
                       ggCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGctgctg                                                                                                                              >  1:4468106/1‑150 (MQ=255)
                         cTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCa                                                                                                                            >  1:3576899/1‑150 (MQ=255)
                           aTCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAat                                                                                                                          >  2:1857895/1‑150 (MQ=255)
                            tCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAata                                                                                                                         <  2:3701855/150‑1 (MQ=255)
                              cGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATAtt                                                                                                                       <  2:644679/150‑1 (MQ=255)
                                ccTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGt                                                                                                                     <  2:188748/150‑1 (MQ=255)
                                ccTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGt                                                                                                                     <  2:3299166/150‑1 (MQ=255)
                                    atgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTcc                                                                                                                 >  1:2501509/1‑150 (MQ=255)
                                     tgaCCACCAAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGA‑TTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTg                                                                                                               <  1:3228203/150‑1 (MQ=255)
                                       accaccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCCCCAGCTGCTGCAATATTGTCTCCTGt                                                                                                              >  2:1255506/1‑150 (MQ=255)
                                       accaccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGt                                                                                                              >  1:126166/1‑150 (MQ=255)
                                             gAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCa                                                                                                        <  1:2937274/150‑1 (MQ=255)
                                             gAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCa                                                                                                        <  2:3523323/150‑1 (MQ=255)
                                             gAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCa                                                                                                        <  2:2061534/150‑1 (MQ=255)
                                               gTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                                      <  2:1995904/150‑1 (MQ=255)
                                                tGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATc                                                                                                     >  2:4697994/1‑150 (MQ=255)
                                                 gcgcGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATcc                                                                                                    <  1:3808823/150‑1 (MQ=255)
                                                    cgGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                                 >  2:1177118/1‑150 (MQ=255)
                                                     ggAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTc                                                                                                <  1:3675862/150‑1 (MQ=255)
                                                         gCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTc                                                                                            >  1:920764/1‑150 (MQ=255)
                                                            cTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAAc                                                                                         <  2:4334845/150‑1 (MQ=255)
                                                            cTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAAc                                                                                         <  1:541269/150‑1 (MQ=255)
                                                                cACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCt                                                                                     >  1:836694/1‑150 (MQ=255)
                                                                cACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCt                                                                                     <  1:2418811/150‑1 (MQ=255)
                                                                 aCTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTg                                                                                    <  1:4250678/150‑1 (MQ=255)
                                                                 aCTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTg                                                                                    >  1:265907/1‑150 (MQ=255)
                                                                 aCTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTg                                                                                    >  1:2283364/1‑150 (MQ=255)
                                                                       gaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGAt                                                                              >  1:117460/1‑150 (MQ=255)
                                                                       gaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGAt                                                                              >  2:2350750/1‑150 (MQ=255)
                                                                           cgcgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAgg                                                                          >  2:1536982/1‑150 (MQ=255)
                                                                             cgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGAc                                                                        >  2:711934/1‑150 (MQ=255)
                                                                              gATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACg                                                                       >  2:2151043/1‑150 (MQ=255)
                                                                              gATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACg                                                                       >  2:2570541/1‑150 (MQ=255)
                                                                              gATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACg                                                                       >  1:3848658/1‑150 (MQ=255)
                                                                                  ccTGCGCAGGCGTTGTGGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAat                                                                   <  2:2674935/150‑1 (MQ=255)
                                                                                      cgcAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATcc                                                               <  1:3172793/150‑1 (MQ=255)
                                                                                       gcAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCg                                                              <  2:2991355/150‑1 (MQ=255)
                                                                                        cAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGa                                                             <  2:2203810/150‑1 (MQ=255)
                                                                                          ggCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACg                                                           <  2:850633/150‑1 (MQ=255)
                                                                                           gCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGc                                                          >  1:1563374/1‑150 (MQ=255)
                                                                                             gttgttGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTa                                                        <  2:4468106/150‑1 (MQ=255)
                                                                                                       cAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAaca                                              >  2:1358304/1‑150 (MQ=255)
                                                                                                        aGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAacac                                             >  1:785300/1‑150 (MQ=255)
                                                                                                         gATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAAcacc                                            <  2:925912/150‑1 (MQ=255)
                                                                                                         gATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAAcacc                                            >  1:3055042/1‑150 (MQ=255)
                                                                                                          aTTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAAcacca                                           >  1:2901959/1‑150 (MQ=255)
                                                                                                               cgcgCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCt                                      <  2:3204423/150‑1 (MQ=255)
                                                                                                               cgcgCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCt                                      <  2:4162849/150‑1 (MQ=255)
                                                                                                               cgcgCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCt                                      >  1:393863/1‑150 (MQ=255)
                                                                                                               cgcgCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCt                                      >  1:1356292/1‑150 (MQ=255)
                                                                                                                   cTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                                  >  2:2337471/1‑150 (MQ=255)
                                                                                                                   cTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                                  >  1:1692258/1‑150 (MQ=255)
                                                                                                                   cTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                                  <  1:1951369/150‑1 (MQ=255)
                                                                                                                    tGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAg                                 <  2:3818411/150‑1 (MQ=255)
                                                                                                                    tGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAg                                 >  2:2388322/1‑150 (MQ=255)
                                                                                                                     gCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGt                                <  1:50610/150‑1 (MQ=255)
                                                                                                                       aataatATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTaa                              >  1:1330263/1‑150 (MQ=255)
                                                                                                                       aataatATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTaa                              >  1:4430928/1‑150 (MQ=255)
                                                                                                                       aataatATCCGGCGCGGCGTTCTTCAGTAGGGTGAACATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTaa                              <  1:278280/150‑1 (MQ=255)
                                                                                                                        ataataTCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAAc                             >  1:4449130/1‑150 (MQ=255)
                                                                                                                        ataataTCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAAc                             >  2:590388/1‑150 (MQ=255)
                                                                                                                         taataTCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCACCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACa                            >  1:1435303/1‑150 (MQ=255)
                                                                                                                          aataTCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACAt                           >  1:2061304/1‑150 (MQ=255)
                                                                                                                            tatCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGc                         <  1:1840350/150‑1 (MQ=255)
                                                                                                                               ccGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCt                      <  1:2054029/150‑1 (MQ=255)
                                                                                                                                cggcgcggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCtt                     >  1:4604267/1‑150 (MQ=255)
                                                                                                                                 ggcgcggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCttt                    >  2:3293528/1‑150 (MQ=255)
                                                                                                                                   cgcggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACAAGCCCTTTTc                  >  1:3313674/1‑150 (MQ=255)
                                                                                                                                   cgcggcgTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTc                  <  2:1692258/150‑1 (MQ=255)
                                                                                                                                     cggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAc                <  1:510825/150‑1 (MQ=255)
                                                                                                                                     cggcgTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAc                <  1:214529/150‑1 (MQ=255)
                                                                                                                                       gcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgc              >  2:3477434/1‑150 (MQ=255)
                                                                                                                                         gTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCgg            <  2:2760743/150‑1 (MQ=255)
                                                                                                                                            cttcAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGgaga         >  1:4518018/1‑150 (MQ=255)
                                                                                                                                             ttcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGaa        <  1:1083858/150‑1 (MQ=255)
                                                                                                                                              tcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAAc       >  1:2372152/1‑150 (MQ=255)
                                                                                                                                              tcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAAc       <  1:427961/150‑1 (MQ=255)
                                                                                                                                               cAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAACg      <  2:2833864/150‑1 (MQ=255)
                                                                                                                                                   cGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAACGcccc  >  2:3113012/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAACGCCCC  >  CP009273/3104100‑3104396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑