Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,120,216 C→A 100% D389Y (GAT→TAT)  glcD ← glycolate oxidase subunit, FAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,120,2160CA95.7% 1006.4 / 30.3 311D389Y (GAT→TAT) glcDglycolate oxidase subunit, FAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (6/8);  new base A (173/124);  total (179/132)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 311 total aligned reads displayed.

TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACG  >  CP009273/3120070‑3120362
                                                                                                                                                  |                                                                                                                                                  
tttttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATcc                                                                                                                                                 <  2:575207/150‑1 (MQ=255)
  tttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCgg                                                                                                                                               >  1:3634911/1‑150 (MQ=255)
  tttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCgg                                                                                                                                               <  2:4566428/150‑1 (MQ=255)
   ttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGc                                                                                                                                              >  1:4257347/1‑150 (MQ=255)
   ttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGc                                                                                                                                              >  2:3099923/1‑150 (MQ=255)
    tctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCa                                                                                                                                             <  2:2387674/150‑1 (MQ=255)
     ctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCAt                                                                                                                                            >  2:2291519/1‑150 (MQ=255)
        gCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGaa                                                                                                                                         <  1:4067361/150‑1 (MQ=255)
        gCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGaa                                                                                                                                         <  2:4247123/150‑1 (MQ=255)
         cccGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGaaa                                                                                                                                        >  2:2800777/1‑150 (MQ=255)
          ccGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAg                                                                                                                                       >  2:398040/1‑150 (MQ=255)
           cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGa                                                                                                                                      >  1:682052/1‑150 (MQ=255)
            gATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGAc                                                                                                                                     >  1:4784284/1‑150 (MQ=255)
            gATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGAc                                                                                                                                     >  2:3048747/1‑150 (MQ=255)
             aTGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACg                                                                                                                                    <  2:3106462/150‑1 (MQ=255)
              tGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGt                                                                                                                                   <  1:2729971/150‑1 (MQ=255)
                tcATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                                 >  2:4692895/2‑150 (MQ=255)
                  aTGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGc                                                                                                                               >  1:1404552/1‑150 (MQ=255)
                  aTGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGc                                                                                                                               >  2:2862520/1‑150 (MQ=255)
                     ttCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAac                                                                                                                            <  2:4027746/150‑1 (MQ=255)
                      tCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAaca                                                                                                                           <  1:1718325/150‑1 (MQ=255)
                          cACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGTTTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTa                                                                                                                       <  1:3807334/150‑1 (MQ=255)
                          cACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTa                                                                                                                       <  1:2357645/150‑1 (MQ=255)
                           aCTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTaa                                                                                                                      >  1:3869707/1‑150 (MQ=255)
                              gATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATc                                                                                                                   >  2:1890867/1‑150 (MQ=255)
                                tgctgcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCat                                                                                                                 <  1:4722395/150‑1 (MQ=255)
                                tgctgcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCat                                                                                                                 <  2:322173/150‑1 (MQ=255)
                                   tgcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATAtt                                                                                                              >  1:2633212/1‑150 (MQ=255)
                                    gccgccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtg                                                                                                             <  1:3912586/150‑1 (MQ=255)
                                    gccgccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtg                                                                                                             <  2:2196422/150‑1 (MQ=255)
                                      cgccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgct                                                                                                           >  2:394728/1‑150 (MQ=255)
                                      cgccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgct                                                                                                           >  2:1376898/1‑150 (MQ=255)
                                      cgccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAAGTCACCGGGGTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgct                                                                                                           <  2:2015374/150‑1 (MQ=255)
                                       gccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctg                                                                                                          <  1:2111699/150‑1 (MQ=255)
                                        ccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctgc                                                                                                         >  2:1139959/1‑150 (MQ=255)
                                         cAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCg                                                                                                        <  2:2272216/150‑1 (MQ=255)
                                          aaCTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGa                                                                                                       >  1:3951706/1‑150 (MQ=255)
                                           aCTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGAt                                                                                                      >  2:4097177/1‑150 (MQ=255)
                                           aCTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGAt                                                                                                      >  2:3285196/1‑150 (MQ=255)
                                             ttCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATaa                                                                                                    >  1:4186636/1‑150 (MQ=255)
                                              tCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATaaa                                                                                                   <  2:4180527/150‑1 (MQ=255)
                                                  cGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACggg                                                                                               <  1:1315336/150‑1 (MQ=255)
                                                   gCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGc                                                                                              >  1:3858243/1‑150 (MQ=255)
                                                    cAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCa                                                                                             <  1:1872653/150‑1 (MQ=255)
                                                       agTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATg                                                                                          <  1:4321111/150‑1 (MQ=255)
                                                       agTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATg                                                                                          >  1:2980173/1‑150 (MQ=255)
                                                          tCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCt                                                                                       <  1:703698/150‑1 (MQ=255)
                                                           cGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCtt                                                                                      <  2:2935803/150‑1 (MQ=255)
                                                            gAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTc                                                                                     >  2:2541976/1‑150 (MQ=255)
                                                            gAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTc                                                                                     >  2:3755765/1‑150 (MQ=255)
                                                            gAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTc                                                                                     >  2:2059389/1‑150 (MQ=255)
                                                              ggATCTTCCCGCCGAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCa                                                                                   <  1:1435075/150‑1 (MQ=255)
                                                              ggATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCa                                                                                   >  1:2213087/1‑150 (MQ=255)
                                                                           cAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcag                                                                      >  2:3560955/1‑150 (MQ=255)
                                                                           cAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcag                                                                      >  1:1682066/1‑150 (MQ=255)
                                                                               tctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGgcg                                                                  >  2:2902429/1‑150 (MQ=255)
                                                                                  tCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGa                                                               >  2:1644441/1‑150 (MQ=255)
                                                                                    cgcgcgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAcg                                                             <  1:2030689/150‑1 (MQ=255)
                                                                                       gcgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCgg                                                          <  1:3290647/150‑1 (MQ=255)
                                                                                          ggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGAt                                                       <  2:558795/150‑1 (MQ=255)
                                                                                             aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    >  2:2984173/1‑150 (MQ=255)
                                                                                              aaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                                   >  1:4371271/1‑150 (MQ=255)
                                                                                               aTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGc                                                  >  2:4484842/1‑150 (MQ=255)
                                                                                                   aCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatc                                              >  2:1178705/1‑150 (MQ=255)
                                                                                                      gggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatccat                                           <  1:4044135/150‑1 (MQ=255)
                                                                                                       ggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATg                                          <  1:3116162/150‑1 (MQ=255)
                                                                                                       ggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATg                                          <  2:4432015/150‑1 (MQ=255)
                                                                                                          tCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCag                                       <  1:961078/150‑1 (MQ=255)
                                                                                                            gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagta                                     >  2:3967306/1‑150 (MQ=255)
                                                                                                             ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtag                                    <  2:3315596/150‑1 (MQ=255)
                                                                                                             ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtag                                    >  1:2315408/1‑150 (MQ=255)
                                                                                                                 cATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAAt                                >  1:3722316/1‑150 (MQ=255)
                                                                                                                 cATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAAt                                >  1:782375/1‑150 (MQ=255)
                                                                                                                  aTCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATc                               <  1:1012977/150‑1 (MQ=255)
                                                                                                                   tCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATcc                              >  1:803483/1‑150 (MQ=255)
                                                                                                                    cGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATcag                             >  1:2266451/1‑148 (MQ=255)
                                                                                                                     gAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                            >  1:4491237/1‑150 (MQ=255)
                                                                                                                       aaaGGATTAACGGGTGCAGGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgggg                          <  2:4734681/150‑1 (MQ=255)
                                                                                                                        aaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGga                         >  1:2195500/1‑150 (MQ=255)
                                                                                                                        aaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGga                         >  1:1352055/1‑150 (MQ=255)
                                                                                                                           gATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                      <  1:1589919/150‑1 (MQ=255)
                                                                                                                            aTTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGTGATa                     >  2:1681810/1‑150 (MQ=255)
                                                                                                                               aaCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGt                  >  2:624193/1‑150 (MQ=255)
                                                                                                                               aaCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGt                  >  1:62996/1‑150 (MQ=255)
                                                                                                                               aaCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGt                  >  1:2457304/1‑150 (MQ=255)
                                                                                                                                 cGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTcc                >  1:534134/1‑150 (MQ=255)
                                                                                                                                  gggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCt               >  2:2498161/1‑150 (MQ=255)
                                                                                                                                   ggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCCGTAGTAATCCGGGGAGATACGTCCTa              >  1:2796980/1‑150 (MQ=255)
                                                                                                                                       cATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgc          >  2:2137173/1‑150 (MQ=255)
                                                                                                                                       cATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgc          >  1:1628611/1‑150 (MQ=255)
                                                                                                                                        aTGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgcc         >  1:3747750/1‑150 (MQ=255)
                                                                                                                                         tGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgccg        <  1:45795/150‑1 (MQ=255)
                                                                                                                                         tGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgccg        >  1:2512382/1‑150 (MQ=255)
                                                                                                                                          gTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCgg       >  2:1087842/1‑150 (MQ=255)
                                                                                                                                          gTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCgg       >  1:1189600/1‑150 (MQ=255)
                                                                                                                                            tGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGa     <  2:3868408/150‑1 (MQ=255)
                                                                                                                                            tGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGa     <  1:2877014/150‑1 (MQ=255)
                                                                                                                                            tGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGa     <  1:487126/150‑1 (MQ=255)
                                                                                                                                              ccATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGacc   >  1:4465291/1‑148 (MQ=255)
                                                                                                                                              ccATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAAc   <  1:4234487/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACG  >  CP009273/3120070‑3120362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑