Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,142,668 G→T 39.5% L55I (CTC→ATC)  yqhA ← UPF0114 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,142,6680GT39.5% 272.0 / 282.0 247L55I (CTC→ATC) yqhAUPF0114 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (71/78);  new base T (38/60);  total (109/138)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 253 total aligned reads displayed.

CGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATTTTCAA  >  CP009273/3142519‑3142817
                                                                                                                                                     |                                                                                                                                                     
cGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAg                                                                                                                                                       <  1:3461169/150‑1 (MQ=255)
   cGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACgatgat                                                                                                                                                    >  2:2212838/1‑150 (MQ=255)
   cGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGAt                                                                                                                                                    <  2:397755/150‑1 (MQ=255)
      tGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCaa                                                                                                                                                 >  1:2817885/1‑150 (MQ=255)
          tCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCt                                                                                                                                             <  2:1727574/150‑1 (MQ=255)
           ccATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTg                                                                                                                                            >  1:3866502/1‑150 (MQ=255)
            cATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGa                                                                                                                                           <  2:4113711/150‑1 (MQ=255)
            cATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                                                                                                                           >  1:964002/1‑150 (MQ=255)
            cATTTTCCCCAGCCAGTTCAGATTCTCTTTGTTATCGGAGATATCCAGCTGTGATACTAAATTCTAATAACCGGAAAACATCACCTTCACAAGCAAACCGCAAACCTGCTTCTTATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                                                                                                                           <  1:2460635/150‑1 (MQ=255)
             aTTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGAt                                                                                                                                          >  2:3438072/1‑150 (MQ=255)
              ttttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGAtt                                                                                                                                         <  2:634905/150‑1 (MQ=255)
              ttttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt                                                                                                                                         >  1:2774033/1‑150 (MQ=255)
                 tCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGTAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCg                                                                                                                                      <  1:4427927/150‑1 (MQ=255)
                   cccAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGcc                                                                                                                                    <  1:4601501/150‑1 (MQ=255)
                    ccagccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCa                                                                                                                                   >  2:3928880/1‑150 (MQ=255)
                     cagccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt                                                                                                                                  >  1:1016554/1‑150 (MQ=255)
                     cagccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt                                                                                                                                  >  1:1015273/1‑150 (MQ=255)
                        ccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCga                                                                                                                               >  2:2581736/1‑150 (MQ=255)
                         cagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                              <  1:4295803/150‑1 (MQ=255)
                          agTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgaga                                                                                                                             >  1:3765794/1‑150 (MQ=255)
                           gTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaa                                                                                                                            >  1:1790369/1‑150 (MQ=255)
                              cAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGat                                                                                                                         >  2:3603172/1‑150 (MQ=255)
                               aGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                        <  2:3802901/150‑1 (MQ=255)
                               aGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                        >  1:2263907/1‑150 (MQ=255)
                               aGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                        <  2:2848525/150‑1 (MQ=255)
                               aGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                        >  1:1975019/1‑150 (MQ=255)
                               aGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                        >  1:3891201/1‑150 (MQ=255)
                                  ttCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTc                                                                                                                     >  1:3437797/1‑150 (MQ=255)
                                   tctcTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg                                                                                                                    <  2:2705927/150‑1 (MQ=255)
                                      ctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCa                                                                                                                 >  2:481023/1‑150 (MQ=255)
                                      ctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCa                                                                                                                 <  2:80686/150‑1 (MQ=255)
                                        ttGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGt                                                                                                               >  2:4509488/1‑150 (MQ=255)
                                         tGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTa                                                                                                              <  1:1364806/150‑1 (MQ=255)
                                         tGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTa                                                                                                              <  1:3724208/150‑1 (MQ=255)
                                          gTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                             >  2:4174422/1‑150 (MQ=255)
                                          gTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                             >  2:1556429/1‑150 (MQ=255)
                                          gTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                             <  2:2901281/150‑1 (MQ=255)
                                          gTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                             <  2:2520402/150‑1 (MQ=255)
                                          gTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc                                                                                                             >  1:439141/1‑150 (MQ=255)
                                            tctcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGt                                                                                                           <  2:1161068/150‑1 (MQ=255)
                                             ctcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTg                                                                                                          <  1:2889275/150‑1 (MQ=255)
                                               cGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                        <  2:334884/150‑1 (MQ=255)
                                               cGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                        <  2:334844/150‑1 (MQ=255)
                                               cGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                        <  2:430056/150‑1 (MQ=255)
                                               cGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaa                                                                                                        >  1:2028520/1‑150 (MQ=255)
                                                ggAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaat                                                                                                       >  2:4376573/1‑150 (MQ=255)
                                                 gagaTATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaata                                                                                                      <  1:3182126/150‑1 (MQ=255)
                                                  agaTATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaataa                                                                                                     <  1:4635679/150‑1 (MQ=255)
                                                    atatCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAAtc                                                                                                   >  2:126695/1‑150 (MQ=255)
                                                      atCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAAtctc                                                                                                 <  1:3060060/150‑1 (MQ=255)
                                                        ccAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCt                                                                                               >  1:2832906/1‑150 (MQ=255)
                                                         cAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTg                                                                                              >  2:2092735/1‑150 (MQ=255)
                                                          aGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATGCGGCAGTACGTGAATAATCTCCTgg                                                                                             >  1:1586035/1‑150 (MQ=255)
                                                               tgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaagaa                                                                                        >  2:1343969/1‑150 (MQ=255)
                                                               tgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaagaa                                                                                        >  1:249660/1‑150 (MQ=255)
                                                               tgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaagaa                                                                                        <  1:1398363/150‑1 (MQ=255)
                                                                  gaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACtt                                                                                     <  1:51526/150‑1 (MQ=255)
                                                                    cGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCa                                                                                   >  1:3250968/1‑150 (MQ=255)
                                                                     gAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAg                                                                                  <  2:4492000/150‑1 (MQ=255)
                                                                               tAACCGGAAAACATCACCATCACCCGCAAACCGCCAACCAGCGTCATATCCACCCGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCa                                                                        <  2:3212434/150‑1 (MQ=255)
                                                                               tAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCa                                                                        >  2:1623955/1‑150 (MQ=255)
                                                                                aaCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCaa                                                                       >  2:2347845/1‑150 (MQ=255)
                                                                                    ggAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTaa                                                                   <  2:2774033/150‑1 (MQ=255)
                                                                                        aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGcc                                                               <  2:2295696/150‑1 (MQ=255)
                                                                                         aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCa                                                              <  1:1896067/150‑1 (MQ=255)
                                                                                         aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCa                                                              <  1:2946989/150‑1 (MQ=255)
                                                                                         aCATCACCATCACAACCAACCCGCCAACCAGCTTAATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCa                                                              <  1:1198817/150‑1 (MQ=255)
                                                                                           atcaccatcaccAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGc                                                            >  1:399677/1‑150 (MQ=255)
                                                                                           atcaccatcaccACCAAACCGCCAACCAGCTTCATATCCACCAGCGACAGCAACACGAGTATCAAATCTTATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAATAACTTCAGCGCCAGGGCAACTAACGCCAGc                                                            <  1:2561079/150‑1 (MQ=255)
                                                                                            tcaccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCg                                                           <  2:3211984/150‑1 (MQ=255)
                                                                                                    accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCa                                                   <  2:3038917/150‑1 (MQ=255)
                                                                                                      caGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCaaa                                                 >  2:1211397/1‑150 (MQ=255)
                                                                                                          aaaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTac                                             <  2:3866590/150‑1 (MQ=255)
                                                                                                            aCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTacac                                           >  2:127855/1‑150 (MQ=255)
                                                                                                            aCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTacac                                           <  2:1061494/150‑1 (MQ=255)
                                                                                                             ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACg                                          >  1:128679/1‑150 (MQ=255)
                                                                                                                  aaCCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGc                                     >  2:1785633/1‑150 (MQ=255)
                                                                                                                   aCCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCa                                    >  1:4234836/1‑150 (MQ=255)
                                                                                                                   aCCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCa                                    <  2:306687/150‑1 (MQ=255)
                                                                                                                     cAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAag                                  >  2:4275498/1‑150 (MQ=255)
                                                                                                                         gTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                              >  2:580456/1‑150 (MQ=255)
                                                                                                                         gTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                              <  2:2411202/150‑1 (MQ=255)
                                                                                                                          tCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagcc                             <  1:1556429/150‑1 (MQ=255)
                                                                                                                               tCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCga                        <  1:1091650/150‑1 (MQ=255)
                                                                                                                               tCCACCAGCGACAGCAACACGAGTATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTTAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCga                        <  1:82659/150‑1 (MQ=255)
                                                                                                                               tCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCga                        >  1:4004320/1‑150 (MQ=255)
                                                                                                                                ccaccaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgag                       <  2:1438640/150‑1 (MQ=255)
                                                                                                                                 caccaGCGACAGCAACACGTGGATCAAATCTGATTCGGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgaga                      <  1:2490864/150‑1 (MQ=255)
                                                                                                                                  accaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGaa                     <  1:202433/150‑1 (MQ=255)
                                                                                                                                  accaGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGaa                     <  2:4644057/150‑1 (MQ=255)
                                                                                                                                      gCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCAt                 <  1:481023/150‑1 (MQ=255)
                                                                                                                                      gCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCAt                 <  1:2355973/150‑1 (MQ=255)
                                                                                                                                        gACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATAc               <  1:386269/150‑1 (MQ=255)
                                                                                                                                         aCAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACa              <  2:530248/150‑1 (MQ=255)
                                                                                                                                          cAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAt             >  2:4547999/1‑150 (MQ=255)
                                                                                                                                                cacGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtt       >  1:782829/1‑150 (MQ=255)
                                                                                                                                                cacGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtt       <  1:3055485/150‑1 (MQ=255)
                                                                                                                                                cacGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtt       <  2:2195853/150‑1 (MQ=255)
                                                                                                                                                   gAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATTTTc    >  1:956363/1‑150 (MQ=255)
                                                                                                                                                     ggATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATTTTCaa  <  2:1701065/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATTTTCAA  >  CP009273/3142519‑3142817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑