Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,237,654 C→A 29.8% M149I (ATG→ATT)  uxaC ← uronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,237,6540CA29.8% 580.7 / 236.9 291M149I (ATG→ATT) uxaCuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (94/110);  new base A (35/52);  total (129/162)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

CGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATA  >  CP009273/3237506‑3237802
                                                                                                                                                    |                                                                                                                                                    
   tAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATct                                                                                                                                                  <  1:2871234/150‑1 (MQ=255)
   tAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATct                                                                                                                                                  >  1:2584030/1‑150 (MQ=255)
    aaaGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctg                                                                                                                                                 >  2:2182186/1‑150 (MQ=255)
    aaaGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATctg                                                                                                                                                 >  2:806023/1‑150 (MQ=255)
           gCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCatg                                                                                                                                          <  1:782657/150‑1 (MQ=255)
           gCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCatg                                                                                                                                          <  1:782848/150‑1 (MQ=255)
             cTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatgat                                                                                                                                        <  1:864393/150‑1 (MQ=255)
             cTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCatgat                                                                                                                                        <  1:1972698/150‑1 (MQ=255)
              tGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCatgatg                                                                                                                                       >  2:1235244/1‑150 (MQ=255)
              tGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCatgatg                                                                                                                                       >  2:778107/1‑150 (MQ=255)
                ttCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGcc                                                                                                                                     >  2:1651214/1‑150 (MQ=255)
                ttCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGcc                                                                                                                                     <  1:4559811/150‑1 (MQ=255)
                ttCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGcc                                                                                                                                     <  1:1128094/150‑1 (MQ=255)
                 tCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCcg                                                                                                                                    >  2:1468145/1‑150 (MQ=255)
                  cGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgc                                                                                                                                   <  2:3925921/150‑1 (MQ=255)
                  cGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgc                                                                                                                                   <  2:1411428/150‑1 (MQ=255)
                   gaggTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcg                                                                                                                                  <  1:1656527/147‑1 (MQ=255)
                     tGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcgcg                                                                                                                                <  1:1429929/150‑1 (MQ=255)
                       ttGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCcgcgcgcg                                                                                                                              <  1:4304621/150‑1 (MQ=255)
                        tGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCgg                                                                                                                             <  2:4053930/150‑1 (MQ=255)
                          aaGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGgag                                                                                                                           <  1:1802752/150‑1 (MQ=255)
                           aGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGgaga                                                                                                                          <  2:1739945/150‑1 (MQ=255)
                            ggCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGaa                                                                                                                         >  2:3813350/1‑150 (MQ=255)
                            ggCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGaa                                                                                                                         >  2:157713/1‑150 (MQ=255)
                             gCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAg                                                                                                                        <  2:2349956/150‑1 (MQ=255)
                                ttGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTa                                                                                                                     <  2:3250594/150‑1 (MQ=255)
                                  gTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGATCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATc                                                                                                                   <  2:4107823/150‑1 (MQ=255)
                                  gTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATc                                                                                                                   <  1:438831/150‑1 (MQ=255)
                                  gTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATc                                                                                                                   <  2:1135179/150‑1 (MQ=255)
                                     cGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTAGGCGATCTCTGCGTGATGCTCCAGAGACTCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTg                                                                                                                >  1:1036185/1‑150 (MQ=255)
                                       gACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTgcg                                                                                                              <  2:3160977/150‑1 (MQ=255)
                                          gCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCca                                                                                                           >  2:2853955/1‑150 (MQ=255)
                                           ccAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcag                                                                                                          <  1:156819/150‑1 (MQ=255)
                                             aGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcagca                                                                                                        >  1:1269837/1‑150 (MQ=255)
                                             aGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCcagca                                                                                                        <  1:2610509/150‑1 (MQ=255)
                                                 cGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTGAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTc                                                                                                    <  2:2984669/150‑1 (MQ=255)
                                                  ggCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCg                                                                                                   >  2:3551538/1‑150 (MQ=255)
                                                      gcaCTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTAc                                                                                               <  2:1625571/150‑1 (MQ=255)
                                                         cTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACAtt                                                                                            >  2:1512510/1‑150 (MQ=255)
                                                          tttGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCGGCAGTTCGTTACATTc                                                                                           >  1:562498/1‑150 (MQ=255)
                                                             gATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTcccc                                                                                        <  1:240533/150‑4 (MQ=255)
                                                             gATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGtt                                                                                        >  2:2207817/1‑150 (MQ=255)
                                                              aTGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTc                                                                                       <  2:4618933/150‑1 (MQ=255)
                                                                  tGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGa                                                                                   <  2:2531480/150‑1 (MQ=255)
                                                                   gAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAt                                                                                  >  2:446487/1‑150 (MQ=255)
                                                                   gAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAt                                                                                  >  2:446469/1‑150 (MQ=255)
                                                                     aaGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAttt                                                                                <  2:3642381/150‑1 (MQ=255)
                                                                     aaGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGAttt                                                                                <  2:4336509/150‑1 (MQ=255)
                                                                      agagCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTc                                                                               <  1:2940827/150‑1 (MQ=255)
                                                                         gCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATc                                                                            <  1:657038/150‑1 (MQ=255)
                                                                           cGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCgg                                                                          >  1:3944513/1‑150 (MQ=255)
                                                                              cTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAg                                                                       >  2:4723430/1‑150 (MQ=255)
                                                                               tttGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGt                                                                      <  2:1967478/150‑1 (MQ=255)
                                                                                  ggCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGa                                                                   <  2:4446036/150‑1 (MQ=255)
                                                                                  ggCGATCTCTGCGTGATGCTCCAGAGAATCAATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGa                                                                   <  1:3451630/150‑1 (MQ=255)
                                                                                    cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                 <  2:2253569/150‑1 (MQ=255)
                                                                                    cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                 >  2:2662326/1‑150 (MQ=255)
                                                                                    cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                 <  2:739237/150‑1 (MQ=255)
                                                                                    cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                 <  1:3067799/150‑1 (MQ=255)
                                                                                    cGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGCTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACg                                                                 >  2:4032629/1‑150 (MQ=255)
                                                                                              gTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCaa                                                       <  1:3284820/150‑1 (MQ=255)
                                                                                               tGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATCTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAt                                                      >  2:4555500/1‑150 (MQ=255)
                                                                                                gATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAAtt                                                     <  1:3813350/150‑1 (MQ=255)
                                                                                                  tGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTg                                                   >  1:941622/1‑150 (MQ=255)
                                                                                                    cTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGcc                                                 <  1:1314070/150‑1 (MQ=255)
                                                                                                    cTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGcc                                                 >  1:2207364/1‑150 (MQ=255)
                                                                                                     tCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCa                                                >  1:2965376/1‑150 (MQ=255)
                                                                                                      ccAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAg                                               >  2:888742/1‑150 (MQ=255)
                                                                                                       cAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGt                                              <  1:3034528/150‑1 (MQ=255)
                                                                                                        agagAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCACTTTGCCAGTg                                             >  2:2617526/1‑150 (MQ=255)
                                                                                                        agagAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTg                                             <  1:2815828/150‑1 (MQ=255)
                                                                                                           gaATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATa                                          >  1:3228758/1‑150 (MQ=255)
                                                                                                            aaTCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAc                                         <  1:4062009/150‑1 (MQ=255)
                                                                                                             atcgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATAcc                                        <  2:547159/150‑1 (MQ=255)
                                                                                                               cgatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCaa                                      <  2:3694172/150‑1 (MQ=255)
                                                                                                                gatcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCaaa                                     >  1:3605310/1‑150 (MQ=255)
                                                                                                                 atcgGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAac                                    <  1:3518600/150‑1 (MQ=255)
                                                                                                                    gggTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacgga                                 <  2:3942323/150‑1 (MQ=255)
                                                                                                                      gTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacg                               <  1:1088101/150‑1 (MQ=255)
                                                                                                                      gTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacg                               <  1:266505/150‑1 (MQ=255)
                                                                                                                      gTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacg                               >  2:1988168/1‑150 (MQ=255)
                                                                                                                      gTCATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacg                               >  1:965818/1‑150 (MQ=255)
                                                                                                                       tCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacgg                              >  2:4179930/1‑150 (MQ=255)
                                                                                                                       tCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAacggacgg                              >  2:3090019/1‑150 (MQ=255)
                                                                                                                        cATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgc                             >  2:800819/1‑150 (MQ=255)
                                                                                                                        cATCGGTGGTGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGgc                             <  1:4469550/150‑1 (MQ=255)
                                                                                                                            ggtggtGCCGACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAg                         >  1:2153018/1‑150 (MQ=255)
                                                                                                                                gtgCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCg                     <  2:3521905/150‑1 (MQ=255)
                                                                                                                                    cGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGt                 >  1:2174193/1‑150 (MQ=255)
                                                                                                                                     gACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTg                >  1:2359716/1‑150 (MQ=255)
                                                                                                                                     gACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTg                >  2:2005227/1‑150 (MQ=255)
                                                                                                                                     gACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACTGACGGCGCAGTTCGAGGTg                >  1:2747354/1‑150 (MQ=255)
                                                                                                                                     gACCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTg                <  2:2929322/150‑1 (MQ=255)
                                                                                                                                      aCCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGc               >  1:810636/1‑150 (MQ=255)
                                                                                                                                      aCCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGc               >  1:3110873/1‑150 (MQ=255)
                                                                                                                                      aCCATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGc               <  1:3691267/150‑1 (MQ=255)
                                                                                                                                        cATTTTCACGTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGt             >  2:632740/1‑150 (MQ=255)
                                                                                                                                                 gTTAATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGta    <  1:2804894/150‑1 (MQ=255)
                                                                                                                                                   tCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGtata  >  2:3292970/1‑150 (MQ=255)
                                                                                                                                                   tCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGtata  <  1:3548823/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTTAAAGGTCGCCTGTTCGATGTTGAAGGCTTTGTCCGGACGCCAGCTCGGCAGCACTTTGATGGTGAAAGAGCCGTCTTTGGCGATCTCTGCGTGATGCTCCAGAGAATCGATCGGGTCATCGGTGGTGCCGACCATTTTCACGTTCATCTGCTGCATGATGCCGCGCGCGGAGAAGTTATCCTGCGCCAGCAGTTCGTTACATTCGTTCCAGATTTCATCGGCAGTTGACGGAGAAAGCAATTTGCCAGTGATACCAAACGGACGGCGCAGTTCGAGGTGCGTCCAGTGGTATA  >  CP009273/3237506‑3237802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑