Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,507,749 G→T 92.7% H224N (CAT→AAT)  rpe ← D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,507,7490GT92.7% 864.0 / 54.7 283H224N (CAT→AAT) rpeD‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (11/10);  new base T (140/122);  total (151/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 284 total aligned reads displayed.

CCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507602‑3507895
                                                                                                                                                   |                                                                                                                                                  
ccAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTAc                                                                                                                                                  >  2:1003892/1‑150 (MQ=255)
 cAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCTTCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACt                                                                                                                                                 <  2:693271/150‑1 (MQ=255)
  tggTAATAACGCTTTCTTCACCTGCGACGGGAAACTCCAGCGCATACAGCGCCATATCTCCCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATAAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACtt                                                                                                                                                <  1:4737711/149‑1 (MQ=255)
  agttaataaCGCGTTCTTCACCTGCGAGGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCTGGAGCACTGTCGACCTGCGTACCATCACGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACtt                                                                                                                                                <  2:1250332/147‑1 (MQ=255)
  aGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACtt                                                                                                                                                >  1:3066090/1‑150 (MQ=255)
      aataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCt                                                                                                                                            <  1:742199/150‑1 (MQ=255)
       ataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACTGCGCCATATCTACCGCTGAAGAAAGACCTGGAGAACTGTCGACCAGCGTACAATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCtt                                                                                                                                           <  1:403188/150‑1 (MQ=255)
         aaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTg                                                                                                                                         >  1:1430055/1‑150 (MQ=255)
           cgcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGcc                                                                                                                                       <  2:2823249/150‑1 (MQ=255)
           cgcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGcc                                                                                                                                       <  2:2715903/150‑1 (MQ=255)
           cgcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGcc                                                                                                                                       <  2:2529734/150‑1 (MQ=255)
           cgcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGcc                                                                                                                                       <  2:1240573/150‑1 (MQ=255)
              gTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGACATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGt                                                                                                                                    <  2:3251468/150‑1 (MQ=255)
               ttcttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGtt                                                                                                                                   <  2:2632714/150‑1 (MQ=255)
                tcttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTc                                                                                                                                  >  1:1295949/1‑150 (MQ=255)
                 cttcACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCa                                                                                                                                 <  1:2371252/150‑1 (MQ=255)
                  ttcACCTGCGTCGGGAAACTCCAGCGCATACAGCGCAATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCTTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCAc                                                                                                                                <  1:4681604/150‑1 (MQ=255)
                  ttcACCTGCGACGGGCAACTCCAGCGAATACAGCGCAATATCTACCGAAGCAGAAAGACCTGGAGCACTGTCGACCTGCTTACCATAAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCAc                                                                                                                                <  1:3004916/150‑1 (MQ=255)
                    cACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCTTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTg                                                                                                                              <  2:870041/150‑1 (MQ=255)
                     aCCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTgc                                                                                                                             >  2:1276628/1‑150 (MQ=255)
                      ccTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTgcg                                                                                                                            <  2:1123119/150‑1 (MQ=255)
                       cTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTgcgc                                                                                                                           >  2:4319548/1‑150 (MQ=255)
                        tGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCa                                                                                                                          >  1:3421384/1‑150 (MQ=255)
                         gCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAt                                                                                                                         <  2:4186707/150‑1 (MQ=255)
                           gACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       <  1:2353381/150‑1 (MQ=255)
                           gACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       >  2:1813483/1‑150 (MQ=255)
                           gACGGGAAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGCCCTGGAGCACTGTCGACCAGCGTACCATCAATATAAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                                                       <  1:4518625/150‑1 (MQ=255)
                              gggCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcat                                                                                                                    >  1:720119/1‑150 (MQ=255)
                               ggCAACTCCAGCGCATACAGCGCCATATCTACCGCCGCAGCAAGACCAGGAGCACTGTCGACCCGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcatc                                                                                                                   <  2:1235750/150‑1 (MQ=255)
                                gCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTtcatca                                                                                                                  >  1:416565/1‑150 (MQ=255)
                                 cAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCTCTGCGCATTTCATCaa                                                                                                                 >  1:3557993/1‑150 (MQ=255)
                                 cAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCaa                                                                                                                 >  1:3638296/1‑150 (MQ=255)
                                  aaCTCCAGCGCATACAGCTAATTATCTACCGCAGCAGAAAGACCAGTAGCACTGTCGACCAGCTTACCATAAATAAAAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAAt                                                                                                                <  2:2395537/150‑1 (MQ=255)
                                     tCCAGCGCATACAGCGCCATATCTCCCGCAGCAGCACGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGAc                                                                                                             <  1:3306993/150‑1 (MQ=255)
                                        aGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACttt                                                                                                          >  1:1162680/1‑150 (MQ=255)
                                              tACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCAGTGTCGACCTGCGTACCATAAAGATAAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTa                                                                                                    <  1:2824163/150‑1 (MQ=255)
                                                  gcgcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                                                                <  2:2176242/150‑1 (MQ=255)
                                                       atatCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTctggctg                                                                                           >  1:2620536/1‑150 (MQ=255)
                                                          tCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTc                                                                                        <  2:2890031/150‑1 (MQ=255)
                                                                gcagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGAACAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAt                                                                                  <  1:773614/150‑1 (MQ=255)
                                                                 cagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAtt                                                                                 >  1:2699356/1‑150 (MQ=255)
                                                                 cagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGAtt                                                                                 <  1:1170621/150‑1 (MQ=255)
                                                                  agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTg                                                                                <  2:2998048/150‑1 (MQ=255)
                                                                     agcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCg                                                                             >  1:1809213/1‑150 (MQ=255)
                                                                         aGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAcc                                                                         <  1:3943680/150‑1 (MQ=255)
                                                                         aGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAAcc                                                                         <  1:1123016/150‑1 (MQ=255)
                                                                              aGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcga                                                                    >  1:1326688/1‑150 (MQ=255)
                                                                              aGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcga                                                                    <  2:3331912/150‑1 (MQ=255)
                                                                                gAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcgacg                                                                  <  2:2795052/150‑1 (MQ=255)
                                                                                 aGCACTGTCGACCAGCGTACCATCATGATCAAACGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcgacga                                                                 <  1:1915376/150‑1 (MQ=255)
                                                                                  gCACTGTCGACCAGCGTACCATCAAGATAAAACGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGaa                                                                <  1:3169316/150‑1 (MQ=255)
                                                                                   cACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAAc                                                               >  2:1685237/1‑150 (MQ=255)
                                                                                    aCTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACa                                                              <  1:204491/150‑1 (MQ=255)
                                                                                      tGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACata                                                            <  2:3051986/150‑1 (MQ=255)
                                                                                         cGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGACCATATcc                                                         >  2:569878/1‑150 (MQ=255)
                                                                                         cGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATcc                                                         <  1:4610886/150‑1 (MQ=255)
                                                                                         cGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATcc                                                         >  1:423748/1‑150 (MQ=255)
                                                                                            ccAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCcgcg                                                      <  1:2529128/150‑1 (MQ=255)
                                                                                              aGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                    >  1:1889874/1‑150 (MQ=255)
                                                                                              aGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc                                                    >  1:1741929/1‑150 (MQ=255)
                                                                                               gCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgccc                                                   <  1:4389856/150‑1 (MQ=255)
                                                                                                cGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccg                                                  <  1:3921622/150‑1 (MQ=255)
                                                                                                     cATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGACCCGGCGACGAACATATCCGCGCCCGCCGCa                                             >  2:3195887/1‑150 (MQ=255)
                                                                                                         aatataaaaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGa                                         <  1:1999511/144‑1 (MQ=255)
                                                                                                         aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCATTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGa                                         <  2:4412302/150‑1 (MQ=255)
                                                                                                           gATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAtt                                       >  1:4334437/1‑150 (MQ=255)
                                                                                                            aTCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAttt                                      <  1:1003892/150‑1 (MQ=255)
                                                                                                            aTCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAttt                                      <  2:2015353/150‑1 (MQ=255)
                                                                                                             tCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTc                                     >  2:3904336/1‑150 (MQ=255)
                                                                                                              cAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCg                                    >  2:3068171/1‑150 (MQ=255)
                                                                                                              cAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCg                                    >  2:4340261/1‑150 (MQ=255)
                                                                                                              cAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCg                                    >  2:4616926/1‑150 (MQ=255)
                                                                                                               aaaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGc                                   >  2:4778680/1‑150 (MQ=255)
                                                                                                                aaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGcc                                  <  2:1032333/150‑1 (MQ=255)
                                                                                                                aaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGcc                                  <  2:1547128/150‑1 (MQ=255)
                                                                                                                aaaGCGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGcc                                  >  1:2031388/1‑150 (MQ=255)
                                                                                                                    cGACGCCGCGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtg                              >  2:1449002/1‑150 (MQ=255)
                                                                                                                        gccgcGAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgttgt                          <  2:1697382/150‑1 (MQ=255)
                                                                                                                            cgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAc                      >  2:3805788/1‑150 (MQ=255)
                                                                                                                            cgAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAc                      <  1:4319548/150‑1 (MQ=255)
                                                                                                                             gAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAcc                     >  2:1926770/1‑150 (MQ=255)
                                                                                                                             gAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAcc                     >  2:2029691/1‑150 (MQ=255)
                                                                                                                             gAATATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCAcc                     >  1:4130354/1‑150 (MQ=255)
                                                                                                                              aaTATCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCt                    >  1:3515268/1‑150 (MQ=255)
                                                                                                                                tatCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTc                  <  2:3148016/150‑1 (MQ=255)
                                                                                                                                tatCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTc                  >  2:3530342/1‑150 (MQ=255)
                                                                                                                                 atCTTCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCCATGTTGTTCACCTTCa                 >  2:4024962/1‑150 (MQ=255)
                                                                                                                                    ttCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGc              >  1:636385/1‑150 (MQ=255)
                                                                                                                                     tCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACCTATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGcc             >  2:2212761/1‑150 (MQ=255)
                                                                                                                                     tCAAACTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGcc             >  1:386408/1‑150 (MQ=255)
                                                                                                                                       aaaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAc           >  1:2838325/1‑150 (MQ=255)
                                                                                                                                        aaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAcc          <  2:2637493/150‑1 (MQ=255)
                                                                                                                                        aaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAcc          <  2:3217547/150‑1 (MQ=255)
                                                                                                                                        aaCTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCAcc          <  1:2543978/150‑1 (MQ=255)
                                                                                                                                          cTTATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGt        >  1:686517/1‑150 (MQ=255)
                                                                                                                                            tATTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTcc      >  1:2734538/1‑150 (MQ=255)
                                                                                                                                             aTTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCa     >  2:3743847/1‑150 (MQ=255)
                                                                                                                                             aTTCATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCa     >  1:1496373/1‑150 (MQ=255)
                                                                                                                                               tCATTACTTACCTTTGCCAGTTCACTGCGCTTTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACt   <  1:4467112/150‑1 (MQ=255)
                                                                                                                                                cATTACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGTCTTTTTTGTAGTCTGGCTGGTCGAAGATTGACGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACtt  >  1:1251143/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTT  >  CP009273/3507602‑3507895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑