Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 21.4% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG21.4% 742.4 / 125.4 416E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (94/226);  new base G (93/3);  total (187/229)
Fisher's exact test for biased strand distribution p-value = 1.24e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 425 total aligned reads displayed.

TCGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCAC  >  CP009273/295546‑295844
                                                                                                                                                     |                                                                                                                                                     
tcgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGtt                                                                                                                                                       <  2:2851922/150‑1 (MQ=255)
 cgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                      >  2:4740211/1‑150 (MQ=255)
     gATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTcccg                                                                                                                                                  >  1:2653149/1‑148 (MQ=255)
     gATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCgg                                                                                                                                                  <  1:3280587/150‑1 (MQ=255)
     gATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCgg                                                                                                                                                  >  2:3466693/1‑150 (MQ=255)
     gATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCgg                                                                                                                                                  >  2:2215956/1‑150 (MQ=255)
     gATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAACCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCgg                                                                                                                                                  >  1:564230/1‑150 (MQ=255)
        cgcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGt                                                                                                                                               <  1:118219/150‑1 (MQ=255)
          cgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGgc                                                                                                                                             >  2:109496/1‑150 (MQ=255)
           gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgcc                                                                                                                                            >  2:3093719/1‑150 (MQ=255)
           gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgcc                                                                                                                                            <  1:852183/150‑1 (MQ=255)
            aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgccg                                                                                                                                           <  1:473455/150‑1 (MQ=255)
            aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgccg                                                                                                                                           <  2:1290420/150‑1 (MQ=255)
            aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCATCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgccg                                                                                                                                           >  2:3374286/1‑150 (MQ=255)
                cGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCgg                                                                                                                                       <  1:2175474/150‑1 (MQ=255)
                  aGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGc                                                                                                                                     <  1:74077/150‑1 (MQ=255)
                   gcgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCa                                                                                                                                    <  1:3895250/150‑1 (MQ=255)
                         tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTc                                                                                                                              <  1:1949226/150‑1 (MQ=255)
                         tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACCGCGGGCCGCCGGGCCGGgttg                                                                                                                              >  1:2561151/1‑149 (MQ=255)
                           aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGTTTCCa                                                                                                                            >  2:3548072/1‑150 (MQ=255)
                           aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCa                                                                                                                            <  1:2023459/150‑1 (MQ=255)
                            gACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCGGGCAGGTTTACAg                                                                                                                           >  2:1201957/1‑150 (MQ=255)
                             aCCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTATGCTGTACCGCCGTTTCCGGCGGGCCGCCGGGGAGGGTTACaaa                                                                                                                          >  2:1996598/1‑148 (MQ=255)
                               cGGGGGCTTCGCCGGGCGCACGCATGGCGGTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGAcc                                                                                                                        <  2:4573007/150‑1 (MQ=255)
                                 ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAg                                                                                                                      <  1:411861/150‑1 (MQ=255)
                                   ggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCCGCGTGCCCCCGGGCCGGGTTACAGACCaaca                                                                                                                    >  1:4777427/1‑147 (MQ=255)
                                    gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCtt                                                                                                                   <  1:428776/150‑1 (MQ=255)
                                    gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCtt                                                                                                                   <  1:4174401/150‑1 (MQ=255)
                                    gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCtt                                                                                                                   <  1:3963278/150‑1 (MQ=255)
                                    gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCtt                                                                                                                   <  1:1063851/150‑1 (MQ=255)
                                    gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCtt                                                                                                                   <  1:3468455/150‑1 (MQ=255)
                                       tCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCa                                                                                                                <  2:3219013/150‑1 (MQ=255)
                                        cGCCGGGCGCACGCCTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTAGGACGAGTCGCCCCGGCGTAGAGTACGTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCAGGGGTCCAGCCCCGcggtcct                                                                                                               >  2:291256/1‑144 (MQ=255)
                                          ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgt                                                                                                             <  2:3161955/150‑1 (MQ=255)
                                          ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgt                                                                                                             <  2:2553823/150‑1 (MQ=255)
                                          ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgt                                                                                                             <  2:4513629/150‑1 (MQ=255)
                                           cGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgtg                                                                                                            <  1:251112/150‑1 (MQ=255)
                                           cGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgtg                                                                                                            <  1:4028628/150‑1 (MQ=255)
                                           cGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGGGGGCCGCCCGGCCGGGTTTCAGACCACCATTAAtgtg                                                                                                            >  1:1677413/1‑150 (MQ=255)
                                            gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCGTTCAtttgt                                                                                                           >  1:3592772/1‑149 (MQ=255)
                                            gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCCGGCAGGTTTCCAGACCAGCATTCATGTga                                                                                                           >  2:1714584/1‑150 (MQ=255)
                                               cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGAt                                                                                                        <  1:992908/150‑1 (MQ=255)
                                                gcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATa                                                                                                       <  1:1567212/150‑1 (MQ=255)
                                                   cgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGGTTCCAGACCAGCTTTCATGTGAGATAGCg                                                                                                    >  2:2385098/1‑150 (MQ=255)
                                                     caTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGTTTGCAGACCAGCTTTCATGTGGGCTATCggg                                                                                                  >  2:3695171/1‑149 (MQ=255)
                                                     caTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGt                                                                                                  <  2:3176171/150‑1 (MQ=255)
                                                      aTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTg                                                                                                 >  2:2518652/1‑150 (MQ=255)
                                                      aTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGTGCCGCCCGGCCGGTTTACAGACCAGCATTAATGTTTGATAGCGGTg                                                                                                 >  1:2355277/1‑150 (MQ=255)
                                                       tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAAGTGAGATAGCGGTGa                                                                                                >  2:2769346/1‑150 (MQ=255)
                                                         gCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAtt                                                                                              <  2:4548831/150‑1 (MQ=255)
                                                            ttCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTc                                                                                           <  2:1886009/150‑1 (MQ=255)
                                                               ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGGCGGGTTCCAGACCAGCTTTCATGTGAGGTTGCGGGGCTTTTGCCg                                                                                        >  2:2474423/1‑150 (MQ=255)
                                                                  tCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGctc                                                                                     <  1:3536515/150‑1 (MQ=255)
                                                                  tCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCGTTCATGTGAGATAGCGGTGCTTTTACCGctc                                                                                     >  2:576216/1‑150 (MQ=255)
                                                                   ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCCGCTTTCATGTGAGATAGCGGTGATTTTACCGctca                                                                                    >  1:2764917/1‑149 (MQ=255)
                                                                     ggCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCATACCAGCGTTCATGTGAGTTAGCGGTGATTTTACCGCTCTgg                                                                                  >  2:1522089/1‑150 (MQ=255)
                                                                      gCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCATTGATGTGAGATAGCGGTGATTTTACCGCTCAGGt                                                                                 >  2:2353493/1‑150 (MQ=255)
                                                                       cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCGCCGGGCCGGGTTCCCGACCAGCGTTACTGTGAGATAGCGGTGATTTTCCCGCTCTGGTc                                                                                >  2:4095541/1‑150 (MQ=255)
                                                                       cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGGGGCGCCGGGCCGGGTTCCACACCCGCGTTCATGTGAGATAGCGGGGATTTTACCCCGCTGGTc                                                                                >  1:798076/1‑150 (MQ=255)
                                                                         aaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCgg                                                                              <  2:1729525/150‑1 (MQ=255)
                                                                          aTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGc                                                                             <  1:1305055/150‑1 (MQ=255)
                                                                             aaGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCAcc                                                                          <  2:224658/150‑1 (MQ=255)
                                                                                 gTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATa                                                                      <  2:1752385/150‑1 (MQ=255)
                                                                                  tGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCCGCGTGCCGCCCGGCCCGGTTCCAGACCACCGGTCATGTGAGATAGCGGTGATTTTACCCCGCGGGTGGGcccccaaaa                                                                     >  1:4260862/1‑145 (MQ=255)
                                                                                      gAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGGCCCCGGGCCGGGTTACAGACCAGCATTGATGTGTGATAGAGGTGTTTTTCACGCACGGGTCGGCCCCCATACGCaa                                                                 >  1:142212/1‑150 (MQ=255)
                                                                                      gAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCCGGCCGGTTTCCCGACCACCATTCCTGTGAGATAGCCGTGATTTTACCGCTCGGGGCGGCCCCCCAACGCaa                                                                 >  2:4447167/1‑150 (MQ=255)
                                                                                        gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTTCCCCCGGGCAGGGTTCCAGACCACCATTCATGTGAGATAGCCGTGATTTTACCGCTCTGGTCCGCACCCAAAAGCaact                                                               >  2:1423677/1‑148 (MQ=255)
                                                                                         ccGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTg                                                              <  1:3060773/150‑1 (MQ=255)
                                                                                          cGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGc                                                             <  2:2093544/150‑1 (MQ=255)
                                                                                           gCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCt                                                            <  1:2216336/150‑1 (MQ=255)
                                                                                              ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGGTTCCAGACCCGCTTTCAGGGGAGCTAGCGGTGAGTTTCCCGCTCTGGTGGGCCCCCCTACGCACGTGCTGaa                                                         >  2:3396320/1‑150 (MQ=255)
                                                                                                ccGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGTTTCCAGACCAGCTTTCAGGTGAGATAGCGGTGATTTTCACGCTCTGGTCGGCACCGCTACGCCAGTGCTGAAgg                                                       >  2:4214932/1‑150 (MQ=255)
                                                                                                 cGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCACCGTTCATGTGTTATAGCGGTGATTTTACCCCTCAGGTCGGCACCCATAACCAAGTGCTGAAggg                                                      >  2:969442/1‑150 (MQ=255)
                                                                                                 cGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTACAGACCAGCGTTCATGTGTGATAGCGGTGTTTTTACCCCTCTGGTCGGCACCCATACGCAAGTGCTGAAggg                                                      >  2:390987/1‑150 (MQ=255)
                                                                                                    tATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTgg                                                   <  2:2464877/150‑1 (MQ=255)
                                                                                                    tATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCCGCTTTCATGTGAGATAGCGGTGATTTTACCGCGCTGGTCGGCACCGATAAGCAAGTGCTGAAgggggg                                                   >  2:664960/1‑150 (MQ=255)
                                                                                                          gATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcggggc                                             <  1:4012392/150‑1 (MQ=255)
                                                                                                                ccccGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGGCGGGCCGGGTTCCAGACCAGCATTGAGGTGAGATGGCGGGGGTTTTACCGGGCGGGTGGGGCCCGAGACGCACGGGGTGTAGGGTgggggggggggggg                                       >  2:4234733/1‑146 (MQ=255)
                                                                                                                   cGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGTGCCCCCGGGCACGTTTACAGACCAGCTTTAATGTGATATAGCGGTGCTTTTACCGCTCGGGTCGGCACCCAAAAGCAACTTCTGAAGGGTGGcggggcggggcgggg                                    >  2:3789741/1‑150 (MQ=255)
                                                                                                                    ggCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCCGGCAGGTTTCCAGACCACCGTTCATGTGAGATATCGGTGATTTTACCGCTCAGGTCGGCACCCATACGCAAGTTCTGAAGGGTGGCGGGGCGGGGCgtggt                                   >  2:1159599/1‑150 (MQ=255)
                                                                                                                     gCGTAGAGTAATTCGCTTTGCTGTACCTCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCAAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCACGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggtg                                  <  1:460492/150‑1 (MQ=255)
                                                                                                                     gCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggtg                                  <  1:1833082/150‑1 (MQ=255)
                                                                                                                       gTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGtt                                <  2:764166/150‑1 (MQ=255)
                                                                                                                         agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAt                              <  1:1030244/150‑1 (MQ=255)
                                                                                                                         agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAt                              <  1:2475439/150‑1 (MQ=255)
                                                                                                                          gagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                             <  1:15258/150‑1 (MQ=255)
                                                                                                                           agTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTg                            <  2:4052417/150‑1 (MQ=255)
                                                                                                                                ttCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                       <  2:342693/150‑1 (MQ=255)
                                                                                                                                 tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                      <  1:664913/150‑1 (MQ=255)
                                                                                                                                 tCGCTTTGCTGTACCGCCGTGTGCGGCGTGGCGCCGGGGCGGTTTGCAGACCAGCTTTAATGTGAGATATCGGGGGTTTTTCCGCTCGGGTCGGGACCGCTACGCAAGTGCTGAAGGGTGGGGGGGCGGGGGGGGGTGTTTTTGGGGATa                      >  2:827191/1‑150 (MQ=255)
                                                                                                                                     tttGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGC‑GGGCGGTGCGTGGTGTTATTGGGAATAGAggg                 <  1:3374286/150‑1 (MQ=255)
                                                                                                                                          tGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATGGAGGGGCgg             <  1:452109/150‑1 (MQ=255)
                                                                                                                                           gTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggg            <  1:3842023/150‑1 (MQ=255)
                                                                                                                                           gTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggg            <  1:3098377/150‑1 (MQ=255)
                                                                                                                                            tACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCgggg           <  1:2100091/150‑1 (MQ=255)
                                                                                                                                            tACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCACCATTCAAGTGAGATATCGGTGATTTTACCGCTCTTGTCGGCACCGAAACGCAAATGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATATATGGGCgggg           >  2:795075/1‑150 (MQ=255)
                                                                                                                                              ccgccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGATTAGAGGGGCGGGGGa         <  1:3749271/150‑1 (MQ=255)
                                                                                                                                                  cgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt     <  2:4685744/150‑1 (MQ=255)
                                                                                                                                                  cgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt     >  2:2073296/1‑150 (MQ=255)
                                                                                                                                                   gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGACGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc    <  2:2689714/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCAC  >  CP009273/295546‑295844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑