BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,718,959	C→A	39.7%	L38F (TTG→TTT)	ysaB ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,718,959	0	C	A	39.7%	304.5 / inf	289	L38F (TTG→TTT)	ysaB	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (80/94); new base A (47/68); total (127/162)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 298 total aligned reads displayed.

TCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTA  >  CP009273/3718810‑3719107

tCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTc                                                                                                                                                      <  1:4691074/150‑1 (MQ=255)

tCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTc                                                                                                                                                      >  2:3646305/1‑150 (MQ=255)

  aaaaGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTCGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTaaa                                                                                                                                                    >  1:1098904/1‑150 (MQ=255)

   aaaGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAaac                                                                                                                                                   >  2:2680643/1‑150 (MQ=255)

        aCAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAaacgaacg                                                                                                                                              <  1:2159393/150‑1 (MQ=255)

        aCAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAaacgaacg                                                                                                                                              <  1:4561878/150‑1 (MQ=255)

         cAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGc                                                                                                                                             <  2:3750929/150‑1 (MQ=255)

          aGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGTAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCa                                                                                                                                            <  1:3512241/150‑1 (MQ=255)

           gACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCag                                                                                                                                           <  2:1416369/150‑1 (MQ=255)

           gACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCag                                                                                                                                           >  2:175578/1‑150 (MQ=255)

           gACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCag                                                                                                                                           <  2:1545769/150‑1 (MQ=255)

              aaaCTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAgg                                                                                                                                        >  1:3066876/1‑150 (MQ=255)

                aCTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCAAAACGCTTCCATATTCAACGAACGCAGAGGAt                                                                                                                                      >  1:2348989/1‑150 (MQ=255)

                  tGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCa                                                                                                                                    >  1:2744784/1‑150 (MQ=255)

                    ctctTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAAc                                                                                                                                  <  1:227002/150‑1 (MQ=255)

                    ctctTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAAc                                                                                                                                  <  1:495078/150‑1 (MQ=255)

                    ctctTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAAc                                                                                                                                  >  2:875594/1‑150 (MQ=255)

                        ttACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCttt                                                                                                                              <  2:3231288/150‑1 (MQ=255)

                           cGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTAcc                                                                                                                           >  1:625606/1‑150 (MQ=255)

                              aaGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGt                                                                                                                        <  1:1656949/150‑1 (MQ=255)

                               aGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGtt                                                                                                                       <  1:3770772/150‑1 (MQ=255)

                                 gTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTtgt                                                                                                                     >  2:4441444/1‑150 (MQ=255)

                                 caataACCGCGCATTTCATAGCTTACCTTGAACTGTTCTAAGGCGGAGAAGTAGATTATTTGCTCGCCGGTGTTATAACGTTTTGAAGCCTGATCCTTGCATAACGCTTCCATAATAAACGAACGCAGACGATCAACCTTAACCCGTagt                                                                                                                     >  2:1062096/3‑150 (MQ=255)

                                    taACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCt                                                                                                                  >  2:4236395/1‑150 (MQ=255)

                                        cgcgCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCt                                                                                                              <  2:793469/150‑1 (MQ=255)

                                        cgcgCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCt                                                                                                              <  1:2527919/150‑1 (MQ=255)

                                         gcgcATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTg                                                                                                             >  2:882491/1‑150 (MQ=255)

                                         gcgcATTTCATAGCGTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTg                                                                                                             <  2:4343209/150‑1 (MQ=255)

                                             aTTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACg                                                                                                         >  2:612745/1‑150 (MQ=255)

                                                tCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGt                                                                                                      >  2:4710195/1‑150 (MQ=255)

                                                tCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGt                                                                                                      <  1:697377/150‑1 (MQ=255)

                                                  aTAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGa                                                                                                    >  2:447960/1‑150 (MQ=255)

                                                  aTAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGa                                                                                                    <  1:3919458/150‑1 (MQ=255)

                                                  aTAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGa                                                                                                    <  1:1506601/150‑1 (MQ=255)

                                                    aGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACg                                                                                                  >  2:1258119/1‑150 (MQ=255)

                                                    aGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACg                                                                                                  >  1:2640396/1‑150 (MQ=255)

                                                      cTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGt                                                                                                >  1:4367375/1‑150 (MQ=255)

                                                       ttCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGta                                                                                               <  2:1908545/150‑1 (MQ=255)

                                                       ttCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGta                                                                                               >  2:3580816/1‑150 (MQ=255)

                                                       ttCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCGTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGta                                                                                               >  1:920367/1‑150 (MQ=255)

                                                       ttCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAAAGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGta                                                                                               >  1:3312298/1‑150 (MQ=255)

                                                       ttCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTGTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGta                                                                                               <  1:1597414/150‑1 (MQ=255)

                                                        tCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGtat                                                                                              <  1:34700/150‑1 (MQ=255)

                                                         cccTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGtata                                                                                             >  2:3681608/1‑150 (MQ=255)

                                                          ccTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAg                                                                                            <  2:4683791/150‑1 (MQ=255)

                                                            tGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGaa                                                                                          >  1:4243838/1‑150 (MQ=255)

                                                            tGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGaa                                                                                          <  1:3030219/150‑1 (MQ=255)

                                                            tGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGaa                                                                                          <  1:339619/150‑1 (MQ=255)

                                                              gAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGaaca                                                                                        <  1:18110/150‑1 (MQ=255)

                                                              gAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGaaca                                                                                        <  1:1081875/150‑1 (MQ=255)

                                                                       gAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAg                                                                               <  1:2139959/150‑1 (MQ=255)

                                                                         aGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCa                                                                             >  1:2710530/1‑150 (MQ=255)

                                                                            cGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCa                                                                          <  1:3121028/150‑1 (MQ=255)

                                                                            cGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCa                                                                          <  2:2387990/150‑1 (MQ=255)

                                                                             ggTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCAt                                                                         >  2:3867496/1‑150 (MQ=255)

                                                                              gTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATa                                                                        <  1:2024979/150‑1 (MQ=255)

                                                                               tGACGTCGATTTTTTTCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAAAGAACGCAGAGGATCAACCTTTACCCGTTTTTCTTTATTCACGGGTGACGGTATATAACAACCCAATAGCACCATaa                                                                       >  1:1254260/1‑150 (MQ=255)

                                                                                gACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAg                                                                      <  2:2430899/150‑1 (MQ=255)

                                                                                   gTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAgcgc                                                                   <  2:518977/150‑1 (MQ=255)

                                                                                   gTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAgcgc                                                                   >  1:1188831/1‑150 (MQ=255)

                                                                                   gTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAgcgc                                                                   <  2:1381215/150‑1 (MQ=255)

                                                                                       aTTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCAtt                                                               <  2:2270545/150‑1 (MQ=255)

                                                                                        ttttttGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTg                                                              <  2:755379/150‑1 (MQ=255)

                                                                                         tttttGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGc                                                             >  1:4631127/1‑150 (MQ=255)

                                                                                          ttttGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGcc                                                            <  2:479386/150‑1 (MQ=255)

                                                                                            ttGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCgg                                                          <  1:3031887/150‑1 (MQ=255)

                                                                                               cTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGgaaa                                                       >  2:3542570/1‑150 (MQ=255)

                                                                                                 cGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATGTTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGgaaaga                                                     >  1:2917162/1‑150 (MQ=255)

                                                                                                   ccGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGgaaagaaa                                                   <  2:1601308/150‑1 (MQ=255)

                                                                                                         ttATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGTCGGAAAGAAAGCGTtc                                             <  1:2305887/150‑1 (MQ=255)

                                                                                                           ataACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTtcat                                           >  2:115383/1‑150 (MQ=255)

                                                                                                            taACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTtcatc                                          >  1:2801985/1‑150 (MQ=255)

                                                                                                            taACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTtcatc                                          >  1:53461/1‑150 (MQ=255)

                                                                                                               cGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTtcatcatc                                       <  1:3970812/150‑1 (MQ=255)

                                                                                                                  tttGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGACAGAAAGCGTTCATCATCAtt                                    >  1:3200265/1‑150 (MQ=255)

                                                                                                                  tttGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCAtt                                    >  2:2470399/1‑150 (MQ=255)

                                                                                                                  tttGCCGCCTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCAtt                                    >  2:669967/1‑150 (MQ=255)

                                                                                                                      ccgccTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAAt                                >  2:4207095/1‑150 (MQ=255)

                                                                                                                      ccgccTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAAt                                <  2:4517029/150‑1 (MQ=255)

                                                                                                                       cgccTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAAtt                               >  1:4185015/1‑150 (MQ=255)

                                                                                                                       cgccTGATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCTCCATAAGCGCCATTGCCGGAAAGACAGCGTTCATCATCATTATAtt                               >  1:4216549/1‑150 (MQ=255)

                                                                                                                        gccTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTa                              >  2:1278213/1‑150 (MQ=255)

                                                                                                                            gATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGt                          >  1:1436112/1‑150 (MQ=255)

                                                                                                                            gATCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGt                          <  2:2842136/150‑1 (MQ=255)

                                                                                                                              tCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCt                        <  2:31630/150‑1 (MQ=255)

                                                                                                                              tCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGATTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGATCATCATCATTAAATTACAGTCt                        >  1:137521/1‑150 (MQ=255)

                                                                                                                              tCCTTGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCt                        >  1:2015862/1‑150 (MQ=255)

                                                                                                                                 ttGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGt                     <  2:3484078/150‑1 (MQ=255)

                                                                                                                                 ttGCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGt                     >  1:471207/1‑150 (MQ=255)

                                                                                                                                  tGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGtt                    <  1:3112437/150‑1 (MQ=255)

                                                                                                                                   gCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGttt                   >  2:3344349/1‑150 (MQ=255)

                                                                                                                                   gCATAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGttt                   <  1:3621757/150‑1 (MQ=255)

                                                                                                                                      tAACGCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCa                <  2:234949/150‑1 (MQ=255)

                                                                                                                                       aaCGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAg               >  2:2172999/1‑150 (MQ=255)

                                                                                                                                         agCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGt             <  1:4495512/149‑1 (MQ=255)

                                                                                                                                          gCTTCCATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTg            <  2:3978695/150‑1 (MQ=255)

                                                                                                                                              ccATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTc        >  2:4093934/1‑150 (MQ=255)

                                                                                                                                               cATATTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCt       >  1:4262172/1‑150 (MQ=255)

                                                                                                                                                 tatTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCtta     >  2:3145183/1‑150 (MQ=255)

                                                                                                                                                 tatTAAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCtta     <  1:90332/150‑1 (MQ=255)

TCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTA  >  CP009273/3718810‑3719107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`