Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,339 A→C 14.2% Q12P (CAA→CCA) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3390AC14.2% 434.8 / 16.8 225Q12P (CAA→CCA) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (112/75);  new base C (33/5);  total (145/80)
Fisher's exact test for biased strand distribution p-value = 1.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488
                                                                                                                                                     |                                                                                                                                                     
 atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCac                                                                                                                                                      >  2:4052534/1‑149 (MQ=255)
  tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAc                                                                                                                                                     >  1:2004168/1‑150 (MQ=255)
    taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACACTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                   <  1:3447679/150‑1 (MQ=255)
         aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTgc                                                                                                                                              >  2:4745329/1‑150 (MQ=255)
         aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTgc                                                                                                                                              >  2:1184823/1‑150 (MQ=255)
          aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                             <  2:3928307/150‑1 (MQ=255)
            tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCa                                                                                                                                           <  2:2281078/150‑1 (MQ=255)
             gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAg                                                                                                                                          >  2:2763431/1‑150 (MQ=255)
             gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAg                                                                                                                                          >  2:1137780/1‑150 (MQ=255)
              cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                                                                                                                         <  2:1506635/150‑1 (MQ=255)
                tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCCGAca                                                                                                                                       >  1:3291233/1‑149 (MQ=255)
                  tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCCGACtaa                                                                                                                                     >  1:1437539/1‑148 (MQ=255)
                  tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCAGACTCa                                                                                                                                     >  1:3846087/1‑150 (MQ=255)
                    tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCaaa                                                                                                                                   >  2:3774709/1‑150 (MQ=255)
                    tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaa                                                                                                                                   >  2:1603333/1‑150 (MQ=255)
                     ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGATGCGCACACACaaaa                                                                                                                                  >  1:2985388/1‑150 (MQ=255)
                      tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAAc                                                                                                                                 >  2:4564416/1‑150 (MQ=255)
                      tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCACACTAAAAAc                                                                                                                                 >  2:2842126/1‑150 (MQ=255)
                       cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                >  2:1633201/1‑150 (MQ=255)
                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCACACTCAAAACac                                                                                                                               >  2:3654292/1‑149 (MQ=255)
                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTCAAAACat                                                                                                                               >  1:4686926/1‑149 (MQ=255)
                            aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTCGCCAACGGTTGAGCACACAAAAAAAAGCGaa                                                                                                                           >  1:1345602/1‑150 (MQ=255)
                             aaTATTTAGATTCGGTTGTGGAAAACTTTTGCTTTTCTCTTGCGGTCAGATAAAATGCCCGCTTTGATACCAGGGTATTGTTTTCGATGTCTGTTTCCACCCTCGTGTCATAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:2022140/150‑1 (MQ=255)
                               tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGAGCCACCGGTTGCTCATAATAAAAACAGCGAACTg                                                                                                                        >  2:2259241/1‑150 (MQ=255)
                                atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCCGAATAAAAAAAGAGAAcata                                                                                                                       >  1:523891/1‑147 (MQ=255)
                                  ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTTCGCCCACACAAAACAGCGAAATGAtt                                                                                                                     >  1:49073/1‑150 (MQ=255)
                                   tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCCAACTGAttt                                                                                                                    >  1:2525375/1‑150 (MQ=255)
                                     gATGAGGTTGTGGCAAACGTTTGCTTGTCTCTTTCGGTCAGCTAAAATGCCCGATTTGCTACCACGGGTTTGTTTTCGAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  <  2:1838515/150‑1 (MQ=255)
                                         aGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAATTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              <  2:2304851/150‑1 (MQ=255)
                                         aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              >  2:1181575/1‑150 (MQ=255)
                                             tgttgaaaaCGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGGTTTGCTACCACGGTATTGTTTTCTTTGTCTGTTTCCACCCTCGTTCCATAATATGCGCAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                          <  2:353054/144‑1 (MQ=255)
                                                   aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                    >  2:1359698/1‑150 (MQ=255)
                                                     aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACGCAAAACAGCGAACTGATTTAACGTATTGAAGATCGTc                                                                                                  >  2:80339/1‑150 (MQ=255)
                                                      cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCGAGTCACAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                 <  2:4221643/150‑1 (MQ=255)
                                                         ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTTCGCAGACTCAAAAAAGCCAACTGAGTTAACGTCTTGAAGATAGTccgcc                                                                                              >  1:2697351/1‑150 (MQ=255)
                                                          tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgccg                                                                                             >  2:4462248/1‑150 (MQ=255)
                                                             ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGCAGATCGTCcgccgcgt                                                                                          >  2:4460094/1‑148 (MQ=255)
                                                             ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAACCGACCAGATTTAACGTCTTGAATATAGTAcgccgcgt                                                                                          >  1:4240050/1‑148 (MQ=255)
                                                              tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACAGTCTTGAAGATCGTCCGCCGCGTc                                                                                         >  2:1794605/1‑150 (MQ=255)
                                                              tttCTCTTACGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCACACACAAAACAGCGAACAAATTTACCGGCATGAAGAACGAccgccccatc                                                                                         >  1:1551628/1‑145 (MQ=255)
                                                               ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCACCCGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCttac                                                                                        >  1:2322446/1‑148 (MQ=255)
                                                               ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGAATCAAAAAAGCGAACAGATTTACCCTCTTTAAGATAGTCCGCCGCTGcc                                                                                        >  1:3839104/1‑150 (MQ=255)
                                                                 ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAGTTACCGTCTTGAAGATCGTCCGCCGCTGCCTc                                                                                      >  2:841007/1‑150 (MQ=255)
                                                                 ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACAGCGAACAGAGTTACCGTCTTGAAGATCGTCCGCCGCGTccgc                                                                                      >  1:2163385/1‑148 (MQ=255)
                                                                  tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAACGTCCGCCGCTTCCTCa                                                                                     >  2:2173/1‑150 (MQ=255)
                                                                  tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGACCTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCa                                                                                     >  2:1941120/1‑150 (MQ=255)
                                                                    tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCCGACTCAAAAAAGCGAACTGTTTTACCGTCTTGAAGTTAGTCCGCCGCTTCCTCaaa                                                                                   >  1:3087448/1‑150 (MQ=255)
                                                                     gtCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACAACGAACGGATTTACCGTCTTGAAGAACGGCCGCCGCGTCCACAAAc                                                                                  >  1:4579664/2‑150 (MQ=255)
                                                                      tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGTGCGCAGACACAAAACAGCCAACTGATTTACCGGCTTGCAGATCGTCCGCCCCTTCCCCAAAcc                                                                                 >  2:1261750/1‑150 (MQ=255)
                                                                        ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCt                                                                               >  1:721298/1‑150 (MQ=255)
                                                                         gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACTCAAACCCTg                                                                              >  2:1560007/1‑150 (MQ=255)
                                                                         gTCAGCTAAAAGGCCCGCTTTGCTACCACGGGTTTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTg                                                                              <  2:3408161/150‑1 (MQ=255)
                                                                          tCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCTGt                                                                             >  2:2279798/1‑150 (MQ=255)
                                                                           cAGCTAAATTGCCCGCTTTGCTACCAGGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  2:694011/150‑1 (MQ=255)
                                                                           cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  2:4682546/150‑1 (MQ=255)
                                                                            cgCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGAGGTCTGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  2:2536076/149‑1 (MQ=255)
                                                                              cTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTgc                                                                         >  2:130538/1‑150 (MQ=255)
                                                                               tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCTTCAACAAAACCTTTTTtcc                                                                        >  2:908819/1‑150 (MQ=255)
                                                                                aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAACGGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCAGTTTgccg                                                                       >  1:2715418/1‑150 (MQ=255)
                                                                                  aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCTTGAAGATAGTACGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                     >  2:3716525/1‑150 (MQ=255)
                                                                                   aTGCCCGGTTTGCTACCTCGGGTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                    <  2:3262626/150‑1 (MQ=255)
                                                                                   aTGCCCGCTTTGCTACCACGGGATGTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                    <  2:484337/150‑1 (MQ=255)
                                                                                    ggCCCGTTTTGCTACCTGGTTTTTTTTTTCTTTTTTTGTTTCCACCCTCGATTAATAAATGGCGCACCCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTg                                                                   <  1:3774709/149‑1 (MQ=255)
                                                                                     gCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCACCAACCCTGTTTTCCGCCAGt                                                                  >  1:4392286/1‑150 (MQ=255)
                                                                                         gCTTTTCTCCCAGGGTTTTGTTTTCGATGTCTGTTACCCCCCGCGCGTCATAAATGGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                              <  1:2613717/150‑1 (MQ=255)
                                                                                          cTTTGCTACCCGGGTTTTGTTTTCGTTGTTTGTTCCCACCCTCTAGTCATAAAAGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                             <  1:1137780/150‑1 (MQ=255)
                                                                                            ttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACTGATTTAAAGTCTTGAATATAGTCCGCCGCTTACACAAACCCTGTTTGGCGCCTGTCAGCaac                                                           >  1:1418019/1‑148 (MQ=255)
                                                                                              gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctg                                                         >  2:3881218/1‑150 (MQ=255)
                                                                                                 aCCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                      <  2:4622438/150‑1 (MQ=255)
                                                                                                     cGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAACCCGTTGCGCCGACTCAAAACAGCGGCATGATTTACCGTCTAGAAGATCGTGCGCCGCTTCCTCAAACACGGGTTGGCGCCTGTAAGCATCTGCTGGCGa                                                  >  1:1451788/1‑150 (MQ=255)
                                                                                                       ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                <  2:4146882/150‑1 (MQ=255)
                                                                                                           tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTATTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCAGTCAGCATCTGCTGGCGATGttag                                            >  2:3204947/1‑148 (MQ=255)
                                                                                                            gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATAGTCCCCCGCGTCCTCAAACCCAGTTTTCCGCCAGTCAGCATCATCAGGCGATTTTCGt                                           >  1:4347336/1‑150 (MQ=255)
                                                                                                            gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAATATCGTCCGCCGCTTACACAAACCCTGTTTGCCGCCTGTCAGCAACTGCAGGCGATGTTCGt                                           >  1:1967417/1‑150 (MQ=255)
                                                                                                            gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGGTTGCCGCCAGTCAGCATCTGCTGGCGAGGTTCGt                                           >  1:4328117/1‑150 (MQ=255)
                                                                                                             ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          <  2:424927/150‑1 (MQ=255)
                                                                                                             ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          >  2:2612482/1‑150 (MQ=255)
                                                                                                             ttttCGATGTCTGTTACCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          <  2:1388409/150‑1 (MQ=255)
                                                                                                                  gATGTCTGTTTCCACCCTCGATTCAGAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                     <  2:567214/150‑1 (MQ=255)
                                                                                                                   aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAAAAGCGAACTGATTTAAAGTCTTGAATATCGTCCGCCCCTTCCTCAAACCCAGTTTGCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                    >  1:4501774/1‑150 (MQ=255)
                                                                                                                    tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACAGCGAAAAGAGTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGGTTTACGCCGGTCAGCAACTGCTGGCGATGTTCGTTGCGGTGa                                   >  1:1755904/1‑150 (MQ=255)
                                                                                                                     gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  >  2:64912/1‑150 (MQ=255)
                                                                                                                      tgtGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                 <  2:1343729/148‑1 (MQ=255)
                                                                                                                      tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                 >  2:1005036/1‑150 (MQ=255)
                                                                                                                       cTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCCGACAAAAAAAAGCGCACTGATTTACCGTCTTGAAGATAGTCCGCCCCGTGCTCAAACCCAGTTTGGCGCCGGTCACCATCTGCTGGCGAGGTTCGTTGCGGTGAtaa                                >  1:2430560/1‑148 (MQ=255)
                                                                                                                       cTGTTACCACCCTCGAGACACAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  2:477724/150‑1 (MQ=255)
                                                                                                                            tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCTGTCAGCATATGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           >  2:953771/1‑150 (MQ=255)
                                                                                                                            tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  2:1080693/150‑1 (MQ=255)
                                                                                                                            tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAACTGCTGGCGATGTTCGTTGCGGGGATCACGCCa                           >  2:1655040/1‑150 (MQ=255)
                                                                                                                            tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACCCCa                           >  2:207064/1‑150 (MQ=255)
                                                                                                                             ccACCCTCTAGTCAGAAAAGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAg                          <  1:2156470/150‑1 (MQ=255)
                                                                                                                               aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                        >  2:4366980/1‑150 (MQ=255)
                                                                                                                                      agtaataaatTGCGCACCCGGTGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa                 <  1:2763431/144‑1 (MQ=255)
                                                                                                                                         cAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  2:2710653/150‑1 (MQ=255)
                                                                                                                                            aacaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  1:1344577/147‑1 (MQ=255)
                                                                                                                                            aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAATATCGTCCGCCGCTTCCTCAAAACCGGTTTGACGCCTGTCAGCATCTGCTGGCGCTGTTCGTTGCGGTGATCACGCCCGCGCTATTAATCTGcc           >  1:3659991/1‑150 (MQ=255)
                                                                                                                                                  cgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc     >  1:686750/1‑150 (MQ=255)
                                                                                                                                                    cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg   >  2:3676581/1‑150 (MQ=255)
                                                                                                                                                    cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg   >  2:2670902/1‑150 (MQ=255)
                                                                                                                                                     aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  1:4493912/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑