Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,340 A→C 9.4% Q12H (CAA→CAC) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3400AC 9.4% 455.2 / 2.9 225Q12H (CAA→CAC) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (138/58);  new base C (7/22);  total (145/80)
Fisher's exact test for biased strand distribution p-value = 3.50e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488
                                                                                                                                                    |                                                                                                                                                    
tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAc                                                                                                                                                     >  1:2004168/1‑150 (MQ=255)
  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                   >  1:3837225/1‑150 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTga                                                                                                                                              >  1:3408161/1‑149 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCa                                                                                                                                           <  2:2281078/150‑1 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCa                                                                                                                                           >  1:2710653/1‑150 (MQ=255)
           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAg                                                                                                                                          >  2:1137780/1‑150 (MQ=255)
            cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                                                                                                                         <  2:1506635/150‑1 (MQ=255)
             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGAc                                                                                                                                        >  2:3211694/1‑150 (MQ=255)
                tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCCGACtaa                                                                                                                                     >  1:1437539/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaa                                                                                                                                   >  2:1603333/1‑150 (MQ=255)
                     cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                >  2:337853/1‑150 (MQ=255)
                     cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                >  2:1633201/1‑150 (MQ=255)
                     aaTTTAAATTTTTAGTTGAGTTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                <  2:123219/149‑1 (MQ=255)
                         tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGcca                                                                                                                            >  2:3440735/1‑148 (MQ=255)
                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTCGCCAACGGTTGAGCACACAAAAAAAAGCGaa                                                                                                                           >  1:1345602/1‑150 (MQ=255)
                             ttttgaGATGAGGATGTGGCAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCAGGGGTTTGTTTTCGTTGTCTGTTTCAACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                        <  2:625811/145‑1 (MQ=255)
                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGAAAAAAAAAAGCGAACAGAtt                                                                                                                     >  1:49132/1‑150 (MQ=255)
                                  agttgtggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCCGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                   <  2:4701584/144‑1 (MQ=255)
                                   gATGAGGTTGTGGCAAACGTTTGCTTGTCTCTTTCGGTCAGCTAAAATGCCCGATTTGCTACCACGGGTTTGTTTTCGAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  <  2:1838515/150‑1 (MQ=255)
                                       aGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAATTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              <  2:2304851/150‑1 (MQ=255)
                                       aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              >  2:1181575/1‑150 (MQ=255)
                                       aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTc                                                                                                              >  2:4005878/1‑150 (MQ=255)
                                           tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                          >  2:3751995/1‑150 (MQ=255)
                                           tgtgGCAAACGTTTGCTTTTCTCTTGCGGTCAGATAAATTGCCCGCTTTGATACCAGGGGTTTGTTTTCGTTGTCTTTTTCCACCCTCGTGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                          <  2:4507691/150‑1 (MQ=255)
                                               gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCATGAAGAt                                                                                                      >  2:4364259/1‑150 (MQ=255)
                                                 aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCCCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                    <  2:2739579/150‑1 (MQ=255)
                                                 aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                    >  2:1359698/1‑150 (MQ=255)
                                                 aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACAGCGAACTGATTTAACGTCATGAATATCg                                                                                                    >  1:3191275/1‑150 (MQ=255)
                                                   aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACGCAAAACAGCGAACTGATTTAACGTATTGAAGATCGTc                                                                                                  >  2:80339/1‑150 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCGAGTCACAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                 <  2:4221643/150‑1 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTTCGCAGACTCAAAAAAGCCAACTGAGTTAACGTCTTGAAGATAGTccgcc                                                                                              >  1:2697351/1‑150 (MQ=255)
                                                        tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgccg                                                                                             >  2:4462248/1‑150 (MQ=255)
                                                          cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAACACCGAACAGATTTACCCGCATGAAGATCGTCCCCCCCt                                                                                           >  1:3452896/1‑150 (MQ=255)
                                                           ttttCTCTTTGGGTCATCTAAATGGCCCGTTTTGCTACCAGGTGTTTTTTTTCTTTGTCTTTTTCCACCCTCGATTCATAAAATGCGCAACCGGGTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                          <  1:2509366/150‑1 (MQ=255)
                                                           ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGCAGATCGTCcgccgcgt                                                                                          >  2:4460094/1‑148 (MQ=255)
                                                           ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAACCGACCAGATTTAACGTCTTGAATATAGTAcgccgcgt                                                                                          >  1:4240050/1‑148 (MQ=255)
                                                            tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACAGTCTTGAAGATCGTCCGCCGCGTc                                                                                         >  2:1794605/1‑150 (MQ=255)
                                                            tttCTCTTACGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCACACACAAAACAGCGAACAAATTTACCGGCATGAAGAACGAccgccccatc                                                                                         >  1:1551628/1‑145 (MQ=255)
                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCACCCGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCttac                                                                                        >  1:2322446/1‑148 (MQ=255)
                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTTAAGATCGTCCGCCGCTTcc                                                                                        >  2:2240525/1‑150 (MQ=255)
                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCGTcc                                                                                        >  2:3918251/1‑150 (MQ=255)
                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCACACACAAAAAAGCGAACAGATTTAACGTCTTGAATATCGTCCGCCGCTTcc                                                                                        >  1:3839198/1‑150 (MQ=255)
                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCCAACGGATTTAGCGGCATGAAGATCCGCCGCCGCGTCCt                                                                                       >  1:4375829/1‑150 (MQ=255)
                                                               ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTc                                                                                      >  2:2691002/1‑150 (MQ=255)
                                                               ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAGTTACCGTCTTGAAGATCGTCCGCCGCTGCCTc                                                                                      >  2:841007/1‑150 (MQ=255)
                                                                tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAACGTCCGCCGCTTCCTCa                                                                                     >  2:2173/1‑150 (MQ=255)
                                                                tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGACCTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCa                                                                                     >  2:1941120/1‑150 (MQ=255)
                                                                   gtCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACAACGAACGGATTTACCGTCTTGAAGAACGGCCGCCGCGTCCACAAAc                                                                                  >  1:4579664/2‑150 (MQ=255)
                                                                    tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGTGCGCAGACACAAAACAGCCAACTGATTTACCGGCTTGCAGATCGTCCGCCCCTTCCCCAAAcc                                                                                 >  2:1261750/1‑150 (MQ=255)
                                                                      ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCt                                                                               >  1:721298/1‑150 (MQ=255)
                                                                       gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTACTCAAACCCTg                                                                              >  2:1560007/1‑150 (MQ=255)
                                                                         cAGCTAAATTGCCCGCTTTGCTACCAGGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  2:694011/150‑1 (MQ=255)
                                                                         cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  2:4682546/150‑1 (MQ=255)
                                                                          agttAAAATGCCCGCTTTTCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTGGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  2:2535714/147‑1 (MQ=255)
                                                                            cTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCGTGAAGATCGGACGCCCCGTCCTCAAAACCAGTTTgc                                                                         >  1:612275/1‑150 (MQ=255)
                                                                            cTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTgc                                                                         >  2:130538/1‑150 (MQ=255)
                                                                             tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCTTCAACAAAACCTTTTTtcc                                                                        >  2:908819/1‑150 (MQ=255)
                                                                                aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                     >  2:2275878/1‑150 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATGTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                    <  2:484337/150‑1 (MQ=255)
                                                                                    cccGCTTTGCTACCAGGGTTTTGTTTTCTTTGTCTGTTTCCACCCTCGAGTCATAAAAGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                 <  1:3033389/150‑1 (MQ=255)
                                                                                    cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                 >  2:3675372/1‑150 (MQ=255)
                                                                                        cTTTGCTACCCGGGTTTTGTTTTCGTTGTTTGTTCCCACCCTCTAGTCATAAAAGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                             <  1:1137780/150‑1 (MQ=255)
                                                                                          ttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACTGATTTAAAGTCTTGAATATAGTCCGCCGCTTACACAAACCCTGTTTGGCGCCTGTCAGCaac                                                           >  1:1418019/1‑148 (MQ=255)
                                                                                           tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCAGCATct                                                          >  2:3681889/1‑150 (MQ=255)
                                                                                            gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAATGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAAACCTGTTTGGCGCCTGTCAGCATctt                                                         >  2:3550978/1‑149 (MQ=255)
                                                                                                   cGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAACCCGTTGCGCCGACTCAAAACAGCGGCATGATTTACCGTCTAGAAGATCGTGCGCCGCTTCCTCAAACACGGGTTGGCGCCTGTAAGCATCTGCTGGCGa                                                  >  1:1451788/1‑150 (MQ=255)
                                                                                                     gtttgtttttCTATGTCTTTTACCACCCTCGAGTCACAAATTGCCCAACGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                <  1:4646273/144‑1 (MQ=255)
                                                                                                     ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                <  2:4146882/150‑1 (MQ=255)
                                                                                                          gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATAGTCCCCCGCGTCCTCAAACCCAGTTTTCCGCCAGTCAGCATCATCAGGCGATTTTCGt                                           >  1:4347336/1‑150 (MQ=255)
                                                                                                          gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAATATCGTCCGCCGCTTACACAAACCCTGTTTGCCGCCTGTCAGCAACTGCAGGCGATGTTCGt                                           >  1:1967417/1‑150 (MQ=255)
                                                                                                          gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGGTTGCCGCCAGTCAGCATCTGCTGGCGAGGTTCGt                                           >  1:4328117/1‑150 (MQ=255)
                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          <  2:424927/150‑1 (MQ=255)
                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          >  2:2611946/1‑150 (MQ=255)
                                                                                                           ttttCGATGTCTGTTACCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          <  2:1388409/150‑1 (MQ=255)
                                                                                                            tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACAGTCTTGAAGATCGTACGCCGCATCCACAAACCCAGTTTGCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTg                                         >  1:1689585/1‑150 (MQ=255)
                                                                                                              tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                       <  2:3157092/150‑1 (MQ=255)
                                                                                                                 aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAAAAGCGAACTGATTTAAAGTCTTGAATATCGTCCGCCCCTTCCTCAAACCCAGTTTGCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                    >  1:4501774/1‑150 (MQ=255)
                                                                                                                   gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  >  2:64912/1‑150 (MQ=255)
                                                                                                                    tgtGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                 <  2:1343729/148‑1 (MQ=255)
                                                                                                                    tatGTATCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCATACACAAAACAGCGAACAGATTTACACACATTAAGATCTTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCAGCAAAAGCAGGCGATGTTCGTTTCGGTGaac                                 >  2:1005385/3‑148 (MQ=255)
                                                                                                                     cTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCCGACAAAAAAAAGCGCACTGATTTACCGTCTTGAAGATAGTCCGCCCCGTGCTCAAACCCAGTTTGGCGCCGGTCACCATCTGCTGGCGAGGTTCGTTGCGGTGAtaa                                >  1:2430560/1‑148 (MQ=255)
                                                                                                                     cTGTTACCACCCTCGAGACACAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  2:477724/150‑1 (MQ=255)
                                                                                                                        tttCCACCCGCGAGACATAAACGGCGCCCCCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCANGc                             <  1:3751995/150‑1 (MQ=255)
                                                                                                                         tcccACACTCGAGTAATAAATTGCGCACCGGGGTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            <  1:2978638/148‑1 (MQ=255)
                                                                                                                          tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCTGTCAGCATATGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           >  2:953771/1‑150 (MQ=255)
                                                                                                                          tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  2:1080693/150‑1 (MQ=255)
                                                                                                                          tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAACTGCTGGCGATGTTCGTTGCGGGGATCACGCCa                           >  2:1655040/1‑150 (MQ=255)
                                                                                                                          tCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACCCCa                           >  2:207064/1‑150 (MQ=255)
                                                                                                                           ccccccTCGAGGCACAAATTGCGCACCCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAg                          <  1:2156455/147‑1 (MQ=255)
                                                                                                                                  cGAGTCATAATCTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  2:3873193/150‑1 (MQ=255)
                                                                                                                                    aGTCAGAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa                 <  2:4604983/150‑1 (MQ=255)
                                                                                                                                      tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc               <  2:2773521/150‑1 (MQ=255)
                                                                                                                                          aacaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  1:1344577/147‑1 (MQ=255)
                                                                                                                                          aaatTGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           <  2:2004168/150‑1 (MQ=255)
                                                                                                                                           aactGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  1:2539801/147‑1 (MQ=255)
                                                                                                                                           aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          >  1:2161917/1‑150 (MQ=255)
                                                                                                                                             aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg        >  1:2730953/1‑150 (MQ=255)
                                                                                                                                                cgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc     >  1:686750/1‑150 (MQ=255)
                                                                                                                                                  cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg   >  2:2670902/1‑150 (MQ=255)
                                                                                                                                                   aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  1:4493912/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑