Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,956,662 T→G 8.1% S310R (AGC→CGC)  gpp ← guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,956,6620TG 8.1% 908.5 / 66.3 297S310R (AGC→CGC) gppguanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base T (165/108);  new base G (15/9);  total (180/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 303 total aligned reads displayed.

ACAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTT  >  CP009273/3956513‑3956811
                                                                                                                                                     |                                                                                                                                                     
aCAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCt                                                                                                                                                       >  1:2836861/1‑150 (MQ=255)
       cTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAata                                                                                                                                                >  2:2472416/1‑150 (MQ=255)
       cTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAata                                                                                                                                                <  2:1628014/150‑1 (MQ=255)
          atgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATcc                                                                                                                                             <  2:2615695/150‑1 (MQ=255)
           tgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCt                                                                                                                                            >  2:4764046/1‑150 (MQ=255)
           tgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCt                                                                                                                                            >  2:3004386/1‑150 (MQ=255)
           tgaGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCt                                                                                                                                            >  2:4042204/1‑150 (MQ=255)
              gCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCt                                                                                                                                         <  1:4590313/150‑1 (MQ=255)
               cAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTc                                                                                                                                        <  1:3046911/150‑1 (MQ=255)
                aaaTCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCg                                                                                                                                       >  1:490593/1‑150 (MQ=255)
                  aTCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGAc                                                                                                                                     >  2:4638351/1‑150 (MQ=255)
                     gcgGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGc                                                                                                                                  >  1:1869370/1‑150 (MQ=255)
                     gcgGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGc                                                                                                                                  <  2:3752957/150‑1 (MQ=255)
                     gcgGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGc                                                                                                                                  >  1:4699886/1‑150 (MQ=255)
                       tgCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCa                                                                                                                                >  2:1932932/2‑150 (MQ=255)
                         cTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCTTTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGa                                                                                                                              <  1:1702686/150‑1 (MQ=255)
                            aTTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGc                                                                                                                           >  1:4325879/1‑150 (MQ=255)
                                 ttCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACAt                                                                                                                      <  1:1143498/150‑1 (MQ=255)
                                          ccATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGAc                                                                                                             >  2:1815526/1‑150 (MQ=255)
                                           cATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGAcc                                                                                                            <  2:4328558/150‑1 (MQ=255)
                                            aTTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCa                                                                                                           <  2:1199131/150‑1 (MQ=255)
                                             ttCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAg                                                                                                          >  1:35410/1‑150 (MQ=255)
                                               cGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGc                                                                                                        >  1:2049225/1‑150 (MQ=255)
                                                gTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGcc                                                                                                       >  2:3626351/1‑150 (MQ=255)
                                                gTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCGGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGcc                                                                                                       >  1:716938/1‑150 (MQ=255)
                                                gTTTTCCACCTGATCGAAGAAGTGAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGcc                                                                                                       <  2:2523709/150‑1 (MQ=255)
                                                 ttttCCACCTGATAGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCt                                                                                                      <  2:1482959/150‑1 (MQ=255)
                                                   ttCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTc                                                                                                    >  2:4234051/1‑150 (MQ=255)
                                                     ccACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCAc                                                                                                  >  2:2336571/1‑150 (MQ=255)
                                                     ccACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCAc                                                                                                  >  2:824591/1‑150 (MQ=255)
                                                     ccACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCAc                                                                                                  >  2:276655/1‑150 (MQ=255)
                                                     ccACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCAc                                                                                                  >  2:2180501/1‑150 (MQ=255)
                                                      cACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACg                                                                                                 >  1:3975345/1‑150 (MQ=255)
                                                         cTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAg                                                                                              <  2:659487/150‑1 (MQ=255)
                                                         cTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAg                                                                                              >  2:854648/1‑150 (MQ=255)
                                                         cTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAg                                                                                              >  1:2184245/1‑150 (MQ=255)
                                                           gATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAgcg                                                                                            >  2:2900177/1‑150 (MQ=255)
                                                             tCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCa                                                                                          >  2:749485/1‑150 (MQ=255)
                                                               gaagaaGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCatc                                                                                        >  1:872801/1‑148 (MQ=255)
                                                               gaagaaGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCAcc                                                                                        >  2:2692779/1‑150 (MQ=255)
                                                                   aagTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCAccgccc                                                                                    <  2:4079503/150‑1 (MQ=255)
                                                                        aGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAg                                                                               <  2:3502183/150‑1 (MQ=255)
                                                                        aGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAg                                                                               <  2:1111011/150‑1 (MQ=255)
                                                                           cGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGt                                                                            <  2:2101026/150‑1 (MQ=255)
                                                                            gCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTc                                                                           <  1:4361342/150‑1 (MQ=255)
                                                                             cAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCa                                                                          <  1:1427573/150‑1 (MQ=255)
                                                                                    ggCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCGGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTg                                                                   <  2:631538/150‑1 (MQ=255)
                                                                                     gCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGa                                                                  <  2:3780104/150‑1 (MQ=255)
                                                                                      cTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGaa                                                                 <  2:4514511/150‑1 (MQ=255)
                                                                                      cTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCGGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGaa                                                                 <  2:2026975/150‑1 (MQ=255)
                                                                                       tACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAat                                                                <  1:366238/150‑1 (MQ=255)
                                                                                       tACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAat                                                                <  2:4480225/150‑1 (MQ=255)
                                                                                        aCGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAata                                                               <  2:1347430/150‑1 (MQ=255)
                                                                                          gcgcTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATAtt                                                             <  1:4234051/150‑1 (MQ=255)
                                                                                          gcgcTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATAtt                                                             >  1:753355/1‑150 (MQ=255)
                                                                                           cgcTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTc                                                            <  1:34071/150‑1 (MQ=255)
                                                                                             cTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAg                                                          >  2:3989120/1‑150 (MQ=255)
                                                                                               gtgCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGtt                                                        <  2:3296316/150‑1 (MQ=255)
                                                                                                tgCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTc                                                       >  1:3517132/1‑150 (MQ=255)
                                                                                                  ccTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCgg                                                     <  1:2908846/150‑1 (MQ=255)
                                                                                                   cTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGt                                                    <  2:560052/150‑1 (MQ=255)
                                                                                                    tGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCGGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTa                                                   >  2:4559557/1‑150 (MQ=255)
                                                                                                    tGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCGGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTa                                                   <  2:2724111/150‑1 (MQ=255)
                                                                                                      aTCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTaaa                                                 >  1:2359203/1‑150 (MQ=255)
                                                                                                       tCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTaaaa                                                <  1:1352872/150‑1 (MQ=255)
                                                                                                              cGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGa                                         <  2:34534/150‑1 (MQ=255)
                                                                                                              cGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGa                                         >  2:1390300/1‑150 (MQ=255)
                                                                                                               gATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGAt                                        >  2:1979031/1‑150 (MQ=255)
                                                                                                                aTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATc                                       >  1:2433124/1‑150 (MQ=255)
                                                                                                                aTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATc                                       >  2:3252830/1‑150 (MQ=255)
                                                                                                                aTCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATc                                       >  1:2263407/1‑150 (MQ=255)
                                                                                                                 tcatcaAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCa                                      <  1:276655/150‑1 (MQ=255)
                                                                                                                 tCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCa                                      <  1:2612682/150‑1 (MQ=255)
                                                                                                                  cATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAg                                     >  1:1637706/1‑150 (MQ=255)
                                                                                                                     aaaGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGAt                                  >  2:68140/1‑150 (MQ=255)
                                                                                                                      aaGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATc                                 >  2:2273729/1‑150 (MQ=255)
                                                                                                                      aaGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATc                                 >  2:3258106/1‑150 (MQ=255)
                                                                                                                       aGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCg                                <  1:2148616/150‑1 (MQ=255)
                                                                                                                         cggcgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGcc                              >  2:1764338/1‑150 (MQ=255)
                                                                                                                            cgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGa                           >  1:2528247/1‑150 (MQ=255)
                                                                                                                            cgTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGa                           <  1:2652708/150‑1 (MQ=255)
                                                                                                                                gAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCAc                       >  1:3811334/1‑150 (MQ=255)
                                                                                                                                  atatTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTc                     >  1:3914087/1‑150 (MQ=255)
                                                                                                                                   tatTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCg                    >  1:4306678/1‑150 (MQ=255)
                                                                                                                                     ttACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCggg                  >  1:4394319/1‑150 (MQ=255)
                                                                                                                                        cGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCTGTAGACCCGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAAGGGCGATCCGGATCGCCAGACCACTCGGGAac               <  1:1516686/150‑1 (MQ=255)
                                                                                                                                           aGCGTACGGCTGCTAATATCCTGCTCGACGGCCAGATGCACCATGCCGTAGACCCGGCCTTCACGCAGCGCACCGCCCGCCTGGGTCATACACTGAATATTCAGTTCGTTAAAAAGGGCGATCTGGATCGCCAGACCACTCGGGAACACt            <  1:545733/150‑1 (MQ=255)
                                                                                                                                           aGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACt            >  1:3118168/1‑150 (MQ=255)
                                                                                                                                             cGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTaa          >  2:3358657/1‑150 (MQ=255)
                                                                                                                                              gTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCTGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAAc         <  2:1780575/150‑1 (MQ=255)
                                                                                                                                              gTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAAc         >  1:1028401/1‑150 (MQ=255)
                                                                                                                                              gTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAAc         >  1:1598532/1‑150 (MQ=255)
                                                                                                                                               tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCTGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACg        <  1:2716720/150‑1 (MQ=255)
                                                                                                                                               tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACg        >  1:3350623/1‑150 (MQ=255)
                                                                                                                                               tACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACg        >  1:3061363/1‑150 (MQ=255)
                                                                                                                                                aCGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGc       >  2:259192/1‑150 (MQ=255)
                                                                                                                                                  ggCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGACCACTAACGCAc     >  2:2501060/1‑150 (MQ=255)
                                                                                                                                                    cTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGt   >  1:1820792/1‑150 (MQ=255)
                                                                                                                                                     tGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTCAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGtt  >  2:666381/1‑150 (MQ=255)
                                                                                                                                                     tGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGtt  >  1:447241/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACAGGCGCTGATGAGCAAATCGCGGCTTATTGCTTCAAGATGCCATTCGTTTTCCACCTGATCGAAGAAGTTAGCCGCAACTTTGGCTACGCGCTGTGCCTGATCAATATCGATCATAAAGCGGCGTTGAATATTACGCAGCGTACGGCTGCGAATATCCTGCTCGACGGCCAGATGCAACATGCCGTAGACCAGGCCTTCACGCAGCGCACCGCCCGCCAGGGTCATACACTGAATATTCAGTTCGGTAAAAATGGCGATCAGGATCGCCAGACCACTCGGGAACACTAACGCACGTT  >  CP009273/3956513‑3956811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑